RESUMO Apesar do reconhecido amadurecimento da Engenharia de Software, a forma como as aplicações... more RESUMO Apesar do reconhecido amadurecimento da Engenharia de Software, a forma como as aplicações no geral vêm sendo desenvolvidas, sem contemplar fatores como manutenabilidade e legibilidade de código, compromete significativamente a sua ...
SPIRIT 2013 checklist: recommended items to address in a clinical trial protocol and related docu... more SPIRIT 2013 checklist: recommended items to address in a clinical trial protocol and related documents. (DOC 122 kb)
Summary COVID-19 can result in severe lung injury. It remained to be determined why diabetic indi... more Summary COVID-19 can result in severe lung injury. It remained to be determined why diabetic individuals with uncontrolled glucose levels are more prone to develop the severe form of COVID-19. The molecular mechanism underlying SARS-CoV-2 infection and what determines the onset of the cytokine storm found in severe COVID-19 patients are unknown. Monocytes/macrophages are the most enriched immune cell types in the lungs of COVID-19 patients and appear to have a central role in the pathogenicity of the disease. These cells adapt their metabolism upon infection and become highly glycolytic, which facilitates SARS-CoV-2 replication. The infection triggers mitochondrial ROS production, which induces stabilization of hypoxia-inducible factor-1α (HIF-1α) and consequently promotes glycolysis. HIF-1α-induced changes in monocyte metabolism by SARS-CoV-2 infection directly inhibit T cell response and reduce epithelial cell survival. Targeting HIF-1ɑ may have great therapeutic potential for the development of novel drugs to treat COVID-19.
Purpose The purpose of this study is to extract psyllium mucilage and evaluate its antioxidant co... more Purpose The purpose of this study is to extract psyllium mucilage and evaluate its antioxidant compounds (in the best extraction condition) and its application in a chocolate drink. Design/methodology/approach First, the extraction by ultrasonic bath was evaluated, followed by water bath extraction, and as there was no difference between the methods, a water bath experimental design was carried out to evaluate the best extraction conditions for psyllium mucilage, having response variables, yield and emulsion stability. A chocolate drink with psyllium mucilage was produced and evaluated in the best extraction condition to compare with xanthan gum. Findings The best extraction conditions for psyllium mucilage were 60°C for 2 h and 1:80 ratio (seed:water). It can be verified that psyllium is rich in antioxidant compounds (0.71 mg GAE/g and 6.67 Mmol ET/g by 2,2′-azinobis (3-ethylbenzthiazoline sulfonic acid-6), and 9.65 Mmol ET/g for ferric reducing antioxidant power), which can greatl...
Skinner defining verbal behavior in 1957 excludes the behavior of listener from this category, ho... more Skinner defining verbal behavior in 1957 excludes the behavior of listener from this category, however, some authors such as Horne and Lowe and also Stemmer suggest that the behavior of listener can be a prerequisite for the acquisition of verbal behavior. Stemmer suggests that the behavior of listener is given by ostensive learning, a pavlovian learning that occurs by pairing of stimuli, and once acquired can be transferred to the behavior of speaker and differentially reinforced. This paper will suggest some similarities between the Stemmer’s approach to what Pavlov called Second Signal System, discussing empirical evidence on the function transference of stimuli in nonhumans and humans in the experimental literature. It will point out to the viability of the Pavlov and Stemmer’s theoretical approach to clarify the behavioral processes underlying the stimulus equivalence studies.
Hypothalamic inflammation is a common feature of experimental obesity. Dietary fats are important... more Hypothalamic inflammation is a common feature of experimental obesity. Dietary fats are important triggers of this process, inducing the activation of toll-like receptor-4 (TLR4) signaling and endoplasmic reticulum stress. Microglia cells, which are the cellular components of the innate immune system in the brain, are expected to play a role in the early activation of diet-induced hypothalamic inflammation. Here, we use bone marrow transplants to generate mice chimeras that express a functional TLR4 in the entire body except in bone marrow-derived cells or only in bone marrow-derived cells. We show that a functional TLR4 in bone marrow-derived cells is required for the complete expression of the diet-induced obese phenotype and for the perpetuation of inflammation in the hypothalamus. In an obesity-prone mouse strain, the chemokine CX3CL1 (fractalkine) is rapidly induced in the neurons of the hypothalamus after the introduction of a high-fat diet. The inhibition of hypothalamic frac...
RESUMO Apesar do reconhecido amadurecimento da Engenharia de Software, a forma como as aplicações... more RESUMO Apesar do reconhecido amadurecimento da Engenharia de Software, a forma como as aplicações no geral vêm sendo desenvolvidas, sem contemplar fatores como manutenabilidade e legibilidade de código, compromete significativamente a sua ...
SPIRIT 2013 checklist: recommended items to address in a clinical trial protocol and related docu... more SPIRIT 2013 checklist: recommended items to address in a clinical trial protocol and related documents. (DOC 122 kb)
Summary COVID-19 can result in severe lung injury. It remained to be determined why diabetic indi... more Summary COVID-19 can result in severe lung injury. It remained to be determined why diabetic individuals with uncontrolled glucose levels are more prone to develop the severe form of COVID-19. The molecular mechanism underlying SARS-CoV-2 infection and what determines the onset of the cytokine storm found in severe COVID-19 patients are unknown. Monocytes/macrophages are the most enriched immune cell types in the lungs of COVID-19 patients and appear to have a central role in the pathogenicity of the disease. These cells adapt their metabolism upon infection and become highly glycolytic, which facilitates SARS-CoV-2 replication. The infection triggers mitochondrial ROS production, which induces stabilization of hypoxia-inducible factor-1α (HIF-1α) and consequently promotes glycolysis. HIF-1α-induced changes in monocyte metabolism by SARS-CoV-2 infection directly inhibit T cell response and reduce epithelial cell survival. Targeting HIF-1ɑ may have great therapeutic potential for the development of novel drugs to treat COVID-19.
Purpose The purpose of this study is to extract psyllium mucilage and evaluate its antioxidant co... more Purpose The purpose of this study is to extract psyllium mucilage and evaluate its antioxidant compounds (in the best extraction condition) and its application in a chocolate drink. Design/methodology/approach First, the extraction by ultrasonic bath was evaluated, followed by water bath extraction, and as there was no difference between the methods, a water bath experimental design was carried out to evaluate the best extraction conditions for psyllium mucilage, having response variables, yield and emulsion stability. A chocolate drink with psyllium mucilage was produced and evaluated in the best extraction condition to compare with xanthan gum. Findings The best extraction conditions for psyllium mucilage were 60°C for 2 h and 1:80 ratio (seed:water). It can be verified that psyllium is rich in antioxidant compounds (0.71 mg GAE/g and 6.67 Mmol ET/g by 2,2′-azinobis (3-ethylbenzthiazoline sulfonic acid-6), and 9.65 Mmol ET/g for ferric reducing antioxidant power), which can greatl...
Skinner defining verbal behavior in 1957 excludes the behavior of listener from this category, ho... more Skinner defining verbal behavior in 1957 excludes the behavior of listener from this category, however, some authors such as Horne and Lowe and also Stemmer suggest that the behavior of listener can be a prerequisite for the acquisition of verbal behavior. Stemmer suggests that the behavior of listener is given by ostensive learning, a pavlovian learning that occurs by pairing of stimuli, and once acquired can be transferred to the behavior of speaker and differentially reinforced. This paper will suggest some similarities between the Stemmer’s approach to what Pavlov called Second Signal System, discussing empirical evidence on the function transference of stimuli in nonhumans and humans in the experimental literature. It will point out to the viability of the Pavlov and Stemmer’s theoretical approach to clarify the behavioral processes underlying the stimulus equivalence studies.
Hypothalamic inflammation is a common feature of experimental obesity. Dietary fats are important... more Hypothalamic inflammation is a common feature of experimental obesity. Dietary fats are important triggers of this process, inducing the activation of toll-like receptor-4 (TLR4) signaling and endoplasmic reticulum stress. Microglia cells, which are the cellular components of the innate immune system in the brain, are expected to play a role in the early activation of diet-induced hypothalamic inflammation. Here, we use bone marrow transplants to generate mice chimeras that express a functional TLR4 in the entire body except in bone marrow-derived cells or only in bone marrow-derived cells. We show that a functional TLR4 in bone marrow-derived cells is required for the complete expression of the diet-induced obese phenotype and for the perpetuation of inflammation in the hypothalamus. In an obesity-prone mouse strain, the chemokine CX3CL1 (fractalkine) is rapidly induced in the neurons of the hypothalamus after the introduction of a high-fat diet. The inhibition of hypothalamic frac...
Uploads