1. Doctoral of philosophy (Ph.D.)/Microbiology/ Molecular Virology, Department of Microbiology, School of Medicine, Aristotle University of Thessaloniki, Greece, 1999.Thesis title: Study of genetic variations of HIV-1 virus in HIV-1/AIDS patients.2. Master of Science (M.Sc.)/Biology with a specialization in Parasitology and Immunology, Yarmouk University, Jordan, 1990.Thesis title: Resistance to secondary Hydatidosis: Effect of dose and type of antigen.3. Bachelor of Science (B.Sc.)/Medical Technology, Yarmouk University, Jordan, 1987.
Curcumin is classified as a chemotherapeutic medication because of its potential against numerous... more Curcumin is classified as a chemotherapeutic medication because of its potential against numerous cancer cell lines and ability to inhibit cancer cell proliferation. Despite these findings, curcumin has yet to be commercialized as a drug due to its low water solubility, low absorption, and restricted bioavailability. As a result, there is a demand for water-soluble curcumin with improved solubility, bioavailability, and thus bioactivity. In this study we report the synthesis and the anticancer activities of water-soluble curcumins derivatives with alkyl sulfonate moiety. The target water-soluble curcumin with alkyl sulfonate moieties was created utilizing a straightforward technique that involved reacting curcumin with various sultones. The cytotoxic (24 h) and cytostatic (72 h) anticancer effect on breast carcinoma (MCF-7), liver carcinoma (HepG2), skin melanoma (B16-F110), colon human cancer and HeLa cervical carcinoma cell lines viability % via MTT assay were determined for the prepared derivatives. Results showed that curcuminderived compounds have a pronounced cytostatic anticancer effect rather than cytotoxic one in relation to the compound type, cancer cell line type, and examined concentration compared to curcumin. The curcumin sulfonates outperformed curcumin activity against the tested cancer cells and showed to be powerful anticancer candidate drugs as supported by the theoretical calculations. This is evident by their high capacity to form H-bonding during docking with the amino acid side chains and the Vina docking score.
Water soluble curcumin with alkyl sulfonate moiety: Synthesis, and anticancer efficacy, 2024
Curcumin is classified as a chemotherapeutic medication because of its potential against numerous... more Curcumin is classified as a chemotherapeutic medication because of its potential against numerous cancer cell lines and ability to inhibit cancer cell proliferation. Despite these findings, curcumin has yet to be commercialized as a drug due to its low water solubility, low absorption, and restricted bioavailability. As a result, there is a demand for water-soluble curcumin with improved solubility, bioavailability, and thus bioactivity. In this study we report the synthesis and the anticancer activities of water-soluble curcumins derivatives with alkyl sulfonate moiety. The target water-soluble curcumin with alkyl sulfonate moieties was created utilizing a straightforward technique that involved reacting curcumin with various sultones. The cytotoxic (24 h) and cytostatic (72 h) anticancer effect on breast carcinoma (MCF-7), liver carcinoma (HepG2), skin melanoma (B16-F110), colon human cancer and HeLa cervical carcinoma cell lines viability % via MTT assay were determined for the prepared derivatives. Results showed that curcuminderived compounds have a pronounced cytostatic anticancer effect rather than cytotoxic one in relation to the compound type, cancer cell line type, and examined concentration compared to curcumin. The curcumin sulfonates outperformed curcumin activity against the tested cancer cells and showed to be powerful anticancer candidate drugs as supported by the theoretical calculations. This is evident by their high capacity to form H-bonding during docking with the amino acid side chains and the Vina docking score.
To evaluate the effectiveness of hepatitis B virus (HBV) vaccination of household contacts of HBV... more To evaluate the effectiveness of hepatitis B virus (HBV) vaccination of household contacts of HBV carriers in Tulkarm district, Palestine, quantitative hepatitis B surface (anti-HBs) antibody response in 161 household contacts was measured after vaccination. A seroprotective anti-HBs response (titre > or = 10 IU/L) was elicited in all vaccinated subjects. Of these 2.5% had titres of 10-99 IU/L, 61.5% 100-999 IU/L and 36.0% > or = 1000 IU/L. The number of vaccination doses had no effect on the achievement of seroprotection. HBV infection was demonstrated in 13 cases and their anti-HBV titres were in the range 25-350 IU/L.
This study aimed to evaluate the relation between the phenotypic antibiotic susceptibility patter... more This study aimed to evaluate the relation between the phenotypic antibiotic susceptibility patterns and the antibiotic resistance genes and to investigate the prevalence of macrolide, lincosamides, streptogramin, aminoglycoside and tetracycline resistance genes among MRSA isolates. A total of 55 clinical MRSA isolates were included in this study, antibiotic resistance was conducted by Kirby-Bauer disk diffusion method, broth microdilution assay and multiplex PCR technique. Our results showed that there was no discordance between conventional susceptibility testing and gene detection by multiplex PCR assay. The prevalence of erm(A), erm(C), tetK, tetM, aacA-aphD, vat(A), vat(B) and vat(C) gene among MRSA isolates was 30.9%, 74.5%, 76.4%, 16.4%, 74.5%, 1.8%, 0% and 5.5%, respectively. These MRSA strains belonged to SCCmec types II, III, IVa and V. Rapid and reliable method for antibiotic susceptibility is important to determine the appropriate therapy decision. Multiplex PCR can be used for confirmation of the results obtained by disk diffusion method or could be used as an alternative diagnostic method in the routine diagnosis for rapid, sensitive, and specific detection of MRSA associated antibiotic resistance genes.
Asian Pacific Journal of Tropical Medicine, Oct 1, 2009
ABSTRACT Objective: This study was conducted to update the prevalence of methicillin-resistant S.... more ABSTRACT Objective: This study was conducted to update the prevalence of methicillin-resistant S. aureus (MRSA) isolates among human clinical S. aureus isolates recovered from Northern Palestine, to evaluate the possible presence of vancomycin-Resistant S. aureus (VRSA) and vancomycin- intermediate resistant S. aureus strains (VISA) and to determine the antimicrobial susceptibilities of these clinical isolates. Methods: The in-vitro activities of 11 antibiotics against 204 non-duplicate S. aureus isolates from clinical samples in North of Palestine were determined by the disk-diffusion method. These samples were isolated between June 2006 and December 2007. The minimum inhibitory concentration (MIC) of vancomycin for 115 methicillin resistant Staphylococcus aureus (MRSA) strains was carried out using the agar dilution method. Results: One hundred and fifteen (56.4%) of these isolates were MRSA and according to their antibiotic profile these are multidrug resistant (resistant to three or more non-β-lactam antibiotics). Ninety nine (43.6%) isolates were methicillin sensitive S. aureus (MSSA), forty four of MSSA isolates (44.4%) were multidrug resistant, while forty five (45.6%) were non multidrug resistant. Our results showed that the most common resistance (95.6%) was to penicillin. Two strains of MRSA have shown to be vancomycin- intermediate resistant, had MIC 4 and 8 μg/ml and these vancomycin- intermediate resistant S. aureus strains (VISA) are resistant to all antibiotics tested. Conclusion: According to our information this is the first study report about VISA in Palestine.
Curcumin is classified as a chemotherapeutic medication because of its potential against numerous... more Curcumin is classified as a chemotherapeutic medication because of its potential against numerous cancer cell lines and ability to inhibit cancer cell proliferation. Despite these findings, curcumin has yet to be commercialized as a drug due to its low water solubility, low absorption, and restricted bioavailability. As a result, there is a demand for water-soluble curcumin with improved solubility, bioavailability, and thus bioactivity. In this study we report the synthesis and the anticancer activities of water-soluble curcumins derivatives with alkyl sulfonate moiety. The target water-soluble curcumin with alkyl sulfonate moieties was created utilizing a straightforward technique that involved reacting curcumin with various sultones. The cytotoxic (24 h) and cytostatic (72 h) anticancer effect on breast carcinoma (MCF-7), liver carcinoma (HepG2), skin melanoma (B16-F110), colon human cancer and HeLa cervical carcinoma cell lines viability % via MTT assay were determined for the prepared derivatives. Results showed that curcuminderived compounds have a pronounced cytostatic anticancer effect rather than cytotoxic one in relation to the compound type, cancer cell line type, and examined concentration compared to curcumin. The curcumin sulfonates outperformed curcumin activity against the tested cancer cells and showed to be powerful anticancer candidate drugs as supported by the theoretical calculations. This is evident by their high capacity to form H-bonding during docking with the amino acid side chains and the Vina docking score.
Water soluble curcumin with alkyl sulfonate moiety: Synthesis, and anticancer efficacy, 2024
Curcumin is classified as a chemotherapeutic medication because of its potential against numerous... more Curcumin is classified as a chemotherapeutic medication because of its potential against numerous cancer cell lines and ability to inhibit cancer cell proliferation. Despite these findings, curcumin has yet to be commercialized as a drug due to its low water solubility, low absorption, and restricted bioavailability. As a result, there is a demand for water-soluble curcumin with improved solubility, bioavailability, and thus bioactivity. In this study we report the synthesis and the anticancer activities of water-soluble curcumins derivatives with alkyl sulfonate moiety. The target water-soluble curcumin with alkyl sulfonate moieties was created utilizing a straightforward technique that involved reacting curcumin with various sultones. The cytotoxic (24 h) and cytostatic (72 h) anticancer effect on breast carcinoma (MCF-7), liver carcinoma (HepG2), skin melanoma (B16-F110), colon human cancer and HeLa cervical carcinoma cell lines viability % via MTT assay were determined for the prepared derivatives. Results showed that curcuminderived compounds have a pronounced cytostatic anticancer effect rather than cytotoxic one in relation to the compound type, cancer cell line type, and examined concentration compared to curcumin. The curcumin sulfonates outperformed curcumin activity against the tested cancer cells and showed to be powerful anticancer candidate drugs as supported by the theoretical calculations. This is evident by their high capacity to form H-bonding during docking with the amino acid side chains and the Vina docking score.
To evaluate the effectiveness of hepatitis B virus (HBV) vaccination of household contacts of HBV... more To evaluate the effectiveness of hepatitis B virus (HBV) vaccination of household contacts of HBV carriers in Tulkarm district, Palestine, quantitative hepatitis B surface (anti-HBs) antibody response in 161 household contacts was measured after vaccination. A seroprotective anti-HBs response (titre > or = 10 IU/L) was elicited in all vaccinated subjects. Of these 2.5% had titres of 10-99 IU/L, 61.5% 100-999 IU/L and 36.0% > or = 1000 IU/L. The number of vaccination doses had no effect on the achievement of seroprotection. HBV infection was demonstrated in 13 cases and their anti-HBV titres were in the range 25-350 IU/L.
This study aimed to evaluate the relation between the phenotypic antibiotic susceptibility patter... more This study aimed to evaluate the relation between the phenotypic antibiotic susceptibility patterns and the antibiotic resistance genes and to investigate the prevalence of macrolide, lincosamides, streptogramin, aminoglycoside and tetracycline resistance genes among MRSA isolates. A total of 55 clinical MRSA isolates were included in this study, antibiotic resistance was conducted by Kirby-Bauer disk diffusion method, broth microdilution assay and multiplex PCR technique. Our results showed that there was no discordance between conventional susceptibility testing and gene detection by multiplex PCR assay. The prevalence of erm(A), erm(C), tetK, tetM, aacA-aphD, vat(A), vat(B) and vat(C) gene among MRSA isolates was 30.9%, 74.5%, 76.4%, 16.4%, 74.5%, 1.8%, 0% and 5.5%, respectively. These MRSA strains belonged to SCCmec types II, III, IVa and V. Rapid and reliable method for antibiotic susceptibility is important to determine the appropriate therapy decision. Multiplex PCR can be used for confirmation of the results obtained by disk diffusion method or could be used as an alternative diagnostic method in the routine diagnosis for rapid, sensitive, and specific detection of MRSA associated antibiotic resistance genes.
Asian Pacific Journal of Tropical Medicine, Oct 1, 2009
ABSTRACT Objective: This study was conducted to update the prevalence of methicillin-resistant S.... more ABSTRACT Objective: This study was conducted to update the prevalence of methicillin-resistant S. aureus (MRSA) isolates among human clinical S. aureus isolates recovered from Northern Palestine, to evaluate the possible presence of vancomycin-Resistant S. aureus (VRSA) and vancomycin- intermediate resistant S. aureus strains (VISA) and to determine the antimicrobial susceptibilities of these clinical isolates. Methods: The in-vitro activities of 11 antibiotics against 204 non-duplicate S. aureus isolates from clinical samples in North of Palestine were determined by the disk-diffusion method. These samples were isolated between June 2006 and December 2007. The minimum inhibitory concentration (MIC) of vancomycin for 115 methicillin resistant Staphylococcus aureus (MRSA) strains was carried out using the agar dilution method. Results: One hundred and fifteen (56.4%) of these isolates were MRSA and according to their antibiotic profile these are multidrug resistant (resistant to three or more non-β-lactam antibiotics). Ninety nine (43.6%) isolates were methicillin sensitive S. aureus (MSSA), forty four of MSSA isolates (44.4%) were multidrug resistant, while forty five (45.6%) were non multidrug resistant. Our results showed that the most common resistance (95.6%) was to penicillin. Two strains of MRSA have shown to be vancomycin- intermediate resistant, had MIC 4 and 8 μg/ml and these vancomycin- intermediate resistant S. aureus strains (VISA) are resistant to all antibiotics tested. Conclusion: According to our information this is the first study report about VISA in Palestine.
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