Hydroxylated polychlorinated biphenyls (OH-PCBs) are well known as metabolites of PCBs in the hum... more Hydroxylated polychlorinated biphenyls (OH-PCBs) are well known as metabolites of PCBs in the human body. We improved a measurement method for OH-PCBs in blood samples using LC/MS/MS. A new 2 microm particle column was used, and the analytical conditions for the LC/MS/MS measurements were optimized. The improved method is more sensitive than the conventional method for 5g blood samples. And, this method is effective at determining the concentrations of OH-PCBs, PCBs and dioxins from the same sample without special treatment of the sample such as derivatization.
Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan, 2021
A microwave digestion method for the analysis of tin, arsenic and lead in soft drinks was studied... more A microwave digestion method for the analysis of tin, arsenic and lead in soft drinks was studied and its performance was evaluated. The trueness, repeatability (RSD) and Intralaboratory reproducibility (RSD) of the method were estimated to be in the ranges of 93-100, 0.7-6.1 and 0.9-8.6%, respectively. In the preparation of the samples for tin analysis, the addition of sulfuric acid after microwave digestion improved the recovery rate and reproducibility. To verify the applicability of this method, recovery tests were conducted on five types of soft drinks, and the results were satisfactory. It was confirmed that this method is applicable to the digestion of a wide range of soft drinks and to the analysis of tin, arsenic, and lead, and that it reduces working time and makes the work easier compared to the conventional method.
Several studies have demonstrated that background-level exposure to environmental chemicals such ... more Several studies have demonstrated that background-level exposure to environmental chemicals such as polychlorinated biphenyls (PCBs) and methyl mercury negatively influences births. However, the effects of background-level exposure to dioxins (PCDDs, PCDFs and coplanar-PCBs) in regard to birth outcomes have not been clarified in a human study. This article presents the effects of prenatal exposure to dioxins on birth weight, while also considering methyl mercury exposure effects. We recruited 514 pregnant women who donated serum specimens and completed a self-administered questionnaire survey. We measured individual isomer levels of dioxins in 270 serum specimens and methyl mercury levels in 430 maternal hair samples. Birth weights correlated negatively with total PCDF levels in the overall sample of infants (r= -0.141; p= 0.024) and particularly in the male subjects (r= -0.241; p= 0.009). Using multiple regression analysis of the association between the levels of dioxins and birth ...
Financing Agreement (Second Education Sector Development Project) between Azerbaijan Republic and... more Financing Agreement (Second Education Sector Development Project) between Azerbaijan Republic and the International Development Association (with schedules, appendix and International Development Association General Conditions for Credits and Grants, dated 1 July 2005, as amended through 15 October 2006). Baku, 24 September 2008
Bioassay-guided investigation of the bark of Elaeocarpus mastersii using KB (human oral epidermoi... more Bioassay-guided investigation of the bark of Elaeocarpus mastersii using KB (human oral epidermoid carcinoma) cells as a monitor led to the isolation of two cucurbitacins, cucurbitacin D and cucurbitacin F as cytotoxic principles, together with two ellagic acid derivatives, 4'-O-methylellagic acid 3-(2",3"-di-O-acetyl)-alpha-L-rhamnoside (1) and 4,4'-O-dimethylellagic acid 3-(2",3"-di-O-acetyl)-alpha-L-rhamnoside (2). These compounds were evaluated against a panel of human tumor cell lines.
We developed an analytical method for accurately determining the concentrations of dioxin and pol... more We developed an analytical method for accurately determining the concentrations of dioxin and polychlorinated biphenyls(PCBs) in a blood volume of 5 g. Inter-laboratory crosscheck is available for maintaining the quality of dioxin and PCB analysis. We carried out the crosschecks of dioxin and PCB analysis in the blood with domestic analysis organizations. Two crosschecks each of dioxin and PCB analysis were carried out from 2007 through 2011. As a result, the findings of our analysis accorded well with the analyses of the other organizations. We believe that our analytical method is sufficiently reliable.
We performed PCB congener specific analysis using HRGC/HRMS and determined their concentrations a... more We performed PCB congener specific analysis using HRGC/HRMS and determined their concentrations and blood chromatogram classifications in 275 individual blood samples collected during annual Yusho examinations between 2007 and 2010. When both blood total PCB concentrations and blood chromatogram classifications were compared among eleven Yusho patients undergoing examinations between 2007 and 2010, the longitudinal concentrations and classifications in the respective individuals hardly changed over these years. In a subset of persons suspected of Yusho, it was found that the mean total blood concentrations of three index congeners, that is, 2, 3', 4, 4', 5-pentaCB (PCB118), 2, 2', 4, 4', 5, 5'-hexaCB (PCB153), 2, 3, 3', 4, 4', 5-hexaCB (PCB156) and mean blood total PCB concentrations gradually decreased between 2007 and 2010. Mean concentration of PCB118 in blood from persons suspected of Yusho was calculated as 0.035 ng/g in the 2010 examination, which w...
A new pregnane glycoside, marsdekoiside A was isolated from the stems of Marsdenia koi (Asclepiad... more A new pregnane glycoside, marsdekoiside A was isolated from the stems of Marsdenia koi (Asclepiadaceae) and its structure was elucidated from chemical and spectral data as 12-cinnamoyl-dihydrosarcostin-3-O-methyl-6-deoxy-beta-D-allopyr ano syl-(1----4)-O-beta-D-oleandropyranosyl-(1----4)-O-beta-D-++ +cymar opyranoside.
Lipid rafts are dynamic assemblies of cholesterol and glycolipid that form detergent-insoluble mi... more Lipid rafts are dynamic assemblies of cholesterol and glycolipid that form detergent-insoluble microdomains within membrane lipid bilayers. Because rafts can be separated by flotation on sucrose gradients, interrogation by mass spectrometry (MS) provides a valuable new insight into lipid raft function. Here we combine liquid chromatography (LC) electrospray ionization (ESI) and matrix-assisted laser desorption ionization (MALDI) MS/MS to corroborate and extend our previous description of lipid raft proteomes derived from the monocytic cell line THP-1. Interestingly, LC-ESI and MALDI MS/MS identify largely non-overlapping, and therefore, potentially complementary protein populations. Using the combined approach, we detected 277 proteins compared to 52 proteins obtained with the original gel-based MALDI MS. We confirmed the presence of 47 of the original 52 proteins demonstrating the consistency of the lipid raft preparations. We demonstrated by immunoblotting that Rac 1 and Rac 2, two of the 52 proteins we failed to confirm, were indeed absent from the lipid raft fractions. The majority of new proteins were cytoskeletal proteins and their regulators, proteins implicated in membrane fusion and vesicular trafficking or signaling molecules. Our results therefore, confirm and extend previous evidence indicating lipid rafts of monocytic cells are specialized for cytoskeletal assembly and vesicle trafficking. Of particular interest, we detected SNAP-23, basigin, Glut-4 and pantophysin in lipid rafts. Since these proteins are implicated in both vesicular trafficking and gamete fusion, lipid rafts may play a common role in these processes. It is evident that the combination of LC-ESI and LC-MALDI MS/MS increases the proteome coverage which allows better understanding of the lipid raft function.
Hydroxylated polychlorinated biphenyls (OH-PCBs) are formed as major metabolites of PCBs by cytoc... more Hydroxylated polychlorinated biphenyls (OH-PCBs) are formed as major metabolites of PCBs by cytochrome P450 enzyme-mediated oxidation. It has been reported that their total concentration in serum samples of Yusho patients ranged from 390 to 1300 pg/g. We measured the concentration of OH-PCBs in blood collected from 183 Yusho patients living in Japan in 2010. The major OH-PCB metabolites were 4-OH-CB187 (ND-1300 pg/g-wet), 4-OH-CB146 + 3-OH-CB153 (8.4-1200 pg/g-wet), 4-OH-CB109 (ND-530 pg/g-wet) and 4'-OH-CB172 (ND-380 pg/g-wet). The total OH-PCBs ranged from 36 to 3800 pg/g-wet. A positive relationship between the concentrations of OH-PCBs and PCBs was observed, but no significant relationship between the concentrations of OH-PCBs and PCDD/DFs was observed.
We measured the concentrations of polychlorinated dizenzo-p-dioxins (PCDDs), polychlorinated dibe... more We measured the concentrations of polychlorinated dizenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and non-ortho coplanar polychlorinated biphenyls (non-ortho PCBs) in blood collected from 242 Yusho patients and 74 Yusho-suspected persons in 2004 and 237 Yusho patients and 114 Yusho-suspected persons in 2005. The sums of toxic equivalents (TEQ) concentrations of PCDDs, PCDFs, and non-ortho PCBs in the blood of Yusho patients in 2004 and 2005 were 126.1 and 124.2 pg TEQ/g lipid, respectively, and the concentrations were 3.4 and 3.3 times higher than those of normal controls that had been previously reported, respectively. Those of the Yusho-suspected persons were about 0.8 and 1.0 times higher than those of normal controls, respectively. Although the TEQ concentrations of PCDDs and non-ortho PCBs among Yusho patients, Yusho-suspected persons, and normal controls were nearly the same, the PCDFs levels of Yusho patients were about 9.8 and 9.5 times higher than those of...
Plants are one of the great resources of the world, and as humankind has evolved, socially, spiri... more Plants are one of the great resources of the world, and as humankind has evolved, socially, spiritually, and economically, we have found, collectively, a myriad uses for the plants around us. One of those uses is for the prevention, treatment, and cure of various disease states. Documented knowledge about such use dates back at least 4000 years, and several of the plant mentioned in the ancient texts remain important drug agents today. Globally, there is now a tremendous interest in examining, scientifically, the basis for the use of various plants in health care systems, and in rationalizing their application through standardized preparations. As a consequence of this heightened interest, the First International Congress on Traditional Medicine and Materia Medica was held in Nov. 2000 in Tehran. It brought together a wide range of participants and offered numerous interesting presentations on the importance of traditional medicine and its evaluation. Following this success, the Sec...
Hydroxylated polychlorinated biphenyls (OH-PCBs) are well known as metabolites of PCBs in the hum... more Hydroxylated polychlorinated biphenyls (OH-PCBs) are well known as metabolites of PCBs in the human body. We improved a measurement method for OH-PCBs in blood samples using LC/MS/MS. A new 2 microm particle column was used, and the analytical conditions for the LC/MS/MS measurements were optimized. The improved method is more sensitive than the conventional method for 5g blood samples. And, this method is effective at determining the concentrations of OH-PCBs, PCBs and dioxins from the same sample without special treatment of the sample such as derivatization.
Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan, 2021
A microwave digestion method for the analysis of tin, arsenic and lead in soft drinks was studied... more A microwave digestion method for the analysis of tin, arsenic and lead in soft drinks was studied and its performance was evaluated. The trueness, repeatability (RSD) and Intralaboratory reproducibility (RSD) of the method were estimated to be in the ranges of 93-100, 0.7-6.1 and 0.9-8.6%, respectively. In the preparation of the samples for tin analysis, the addition of sulfuric acid after microwave digestion improved the recovery rate and reproducibility. To verify the applicability of this method, recovery tests were conducted on five types of soft drinks, and the results were satisfactory. It was confirmed that this method is applicable to the digestion of a wide range of soft drinks and to the analysis of tin, arsenic, and lead, and that it reduces working time and makes the work easier compared to the conventional method.
Several studies have demonstrated that background-level exposure to environmental chemicals such ... more Several studies have demonstrated that background-level exposure to environmental chemicals such as polychlorinated biphenyls (PCBs) and methyl mercury negatively influences births. However, the effects of background-level exposure to dioxins (PCDDs, PCDFs and coplanar-PCBs) in regard to birth outcomes have not been clarified in a human study. This article presents the effects of prenatal exposure to dioxins on birth weight, while also considering methyl mercury exposure effects. We recruited 514 pregnant women who donated serum specimens and completed a self-administered questionnaire survey. We measured individual isomer levels of dioxins in 270 serum specimens and methyl mercury levels in 430 maternal hair samples. Birth weights correlated negatively with total PCDF levels in the overall sample of infants (r= -0.141; p= 0.024) and particularly in the male subjects (r= -0.241; p= 0.009). Using multiple regression analysis of the association between the levels of dioxins and birth ...
Financing Agreement (Second Education Sector Development Project) between Azerbaijan Republic and... more Financing Agreement (Second Education Sector Development Project) between Azerbaijan Republic and the International Development Association (with schedules, appendix and International Development Association General Conditions for Credits and Grants, dated 1 July 2005, as amended through 15 October 2006). Baku, 24 September 2008
Bioassay-guided investigation of the bark of Elaeocarpus mastersii using KB (human oral epidermoi... more Bioassay-guided investigation of the bark of Elaeocarpus mastersii using KB (human oral epidermoid carcinoma) cells as a monitor led to the isolation of two cucurbitacins, cucurbitacin D and cucurbitacin F as cytotoxic principles, together with two ellagic acid derivatives, 4'-O-methylellagic acid 3-(2",3"-di-O-acetyl)-alpha-L-rhamnoside (1) and 4,4'-O-dimethylellagic acid 3-(2",3"-di-O-acetyl)-alpha-L-rhamnoside (2). These compounds were evaluated against a panel of human tumor cell lines.
We developed an analytical method for accurately determining the concentrations of dioxin and pol... more We developed an analytical method for accurately determining the concentrations of dioxin and polychlorinated biphenyls(PCBs) in a blood volume of 5 g. Inter-laboratory crosscheck is available for maintaining the quality of dioxin and PCB analysis. We carried out the crosschecks of dioxin and PCB analysis in the blood with domestic analysis organizations. Two crosschecks each of dioxin and PCB analysis were carried out from 2007 through 2011. As a result, the findings of our analysis accorded well with the analyses of the other organizations. We believe that our analytical method is sufficiently reliable.
We performed PCB congener specific analysis using HRGC/HRMS and determined their concentrations a... more We performed PCB congener specific analysis using HRGC/HRMS and determined their concentrations and blood chromatogram classifications in 275 individual blood samples collected during annual Yusho examinations between 2007 and 2010. When both blood total PCB concentrations and blood chromatogram classifications were compared among eleven Yusho patients undergoing examinations between 2007 and 2010, the longitudinal concentrations and classifications in the respective individuals hardly changed over these years. In a subset of persons suspected of Yusho, it was found that the mean total blood concentrations of three index congeners, that is, 2, 3', 4, 4', 5-pentaCB (PCB118), 2, 2', 4, 4', 5, 5'-hexaCB (PCB153), 2, 3, 3', 4, 4', 5-hexaCB (PCB156) and mean blood total PCB concentrations gradually decreased between 2007 and 2010. Mean concentration of PCB118 in blood from persons suspected of Yusho was calculated as 0.035 ng/g in the 2010 examination, which w...
A new pregnane glycoside, marsdekoiside A was isolated from the stems of Marsdenia koi (Asclepiad... more A new pregnane glycoside, marsdekoiside A was isolated from the stems of Marsdenia koi (Asclepiadaceae) and its structure was elucidated from chemical and spectral data as 12-cinnamoyl-dihydrosarcostin-3-O-methyl-6-deoxy-beta-D-allopyr ano syl-(1----4)-O-beta-D-oleandropyranosyl-(1----4)-O-beta-D-++ +cymar opyranoside.
Lipid rafts are dynamic assemblies of cholesterol and glycolipid that form detergent-insoluble mi... more Lipid rafts are dynamic assemblies of cholesterol and glycolipid that form detergent-insoluble microdomains within membrane lipid bilayers. Because rafts can be separated by flotation on sucrose gradients, interrogation by mass spectrometry (MS) provides a valuable new insight into lipid raft function. Here we combine liquid chromatography (LC) electrospray ionization (ESI) and matrix-assisted laser desorption ionization (MALDI) MS/MS to corroborate and extend our previous description of lipid raft proteomes derived from the monocytic cell line THP-1. Interestingly, LC-ESI and MALDI MS/MS identify largely non-overlapping, and therefore, potentially complementary protein populations. Using the combined approach, we detected 277 proteins compared to 52 proteins obtained with the original gel-based MALDI MS. We confirmed the presence of 47 of the original 52 proteins demonstrating the consistency of the lipid raft preparations. We demonstrated by immunoblotting that Rac 1 and Rac 2, two of the 52 proteins we failed to confirm, were indeed absent from the lipid raft fractions. The majority of new proteins were cytoskeletal proteins and their regulators, proteins implicated in membrane fusion and vesicular trafficking or signaling molecules. Our results therefore, confirm and extend previous evidence indicating lipid rafts of monocytic cells are specialized for cytoskeletal assembly and vesicle trafficking. Of particular interest, we detected SNAP-23, basigin, Glut-4 and pantophysin in lipid rafts. Since these proteins are implicated in both vesicular trafficking and gamete fusion, lipid rafts may play a common role in these processes. It is evident that the combination of LC-ESI and LC-MALDI MS/MS increases the proteome coverage which allows better understanding of the lipid raft function.
Hydroxylated polychlorinated biphenyls (OH-PCBs) are formed as major metabolites of PCBs by cytoc... more Hydroxylated polychlorinated biphenyls (OH-PCBs) are formed as major metabolites of PCBs by cytochrome P450 enzyme-mediated oxidation. It has been reported that their total concentration in serum samples of Yusho patients ranged from 390 to 1300 pg/g. We measured the concentration of OH-PCBs in blood collected from 183 Yusho patients living in Japan in 2010. The major OH-PCB metabolites were 4-OH-CB187 (ND-1300 pg/g-wet), 4-OH-CB146 + 3-OH-CB153 (8.4-1200 pg/g-wet), 4-OH-CB109 (ND-530 pg/g-wet) and 4'-OH-CB172 (ND-380 pg/g-wet). The total OH-PCBs ranged from 36 to 3800 pg/g-wet. A positive relationship between the concentrations of OH-PCBs and PCBs was observed, but no significant relationship between the concentrations of OH-PCBs and PCDD/DFs was observed.
We measured the concentrations of polychlorinated dizenzo-p-dioxins (PCDDs), polychlorinated dibe... more We measured the concentrations of polychlorinated dizenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and non-ortho coplanar polychlorinated biphenyls (non-ortho PCBs) in blood collected from 242 Yusho patients and 74 Yusho-suspected persons in 2004 and 237 Yusho patients and 114 Yusho-suspected persons in 2005. The sums of toxic equivalents (TEQ) concentrations of PCDDs, PCDFs, and non-ortho PCBs in the blood of Yusho patients in 2004 and 2005 were 126.1 and 124.2 pg TEQ/g lipid, respectively, and the concentrations were 3.4 and 3.3 times higher than those of normal controls that had been previously reported, respectively. Those of the Yusho-suspected persons were about 0.8 and 1.0 times higher than those of normal controls, respectively. Although the TEQ concentrations of PCDDs and non-ortho PCBs among Yusho patients, Yusho-suspected persons, and normal controls were nearly the same, the PCDFs levels of Yusho patients were about 9.8 and 9.5 times higher than those of...
Plants are one of the great resources of the world, and as humankind has evolved, socially, spiri... more Plants are one of the great resources of the world, and as humankind has evolved, socially, spiritually, and economically, we have found, collectively, a myriad uses for the plants around us. One of those uses is for the prevention, treatment, and cure of various disease states. Documented knowledge about such use dates back at least 4000 years, and several of the plant mentioned in the ancient texts remain important drug agents today. Globally, there is now a tremendous interest in examining, scientifically, the basis for the use of various plants in health care systems, and in rationalizing their application through standardized preparations. As a consequence of this heightened interest, the First International Congress on Traditional Medicine and Materia Medica was held in Nov. 2000 in Tehran. It brought together a wide range of participants and offered numerous interesting presentations on the importance of traditional medicine and its evaluation. Following this success, the Sec...
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Papers by Geoffrey Cordell