<p>Differential leukocyte counts (A), CD4:CD8 ratios (B) and PBMC leukocyte subpopulations ... more <p>Differential leukocyte counts (A), CD4:CD8 ratios (B) and PBMC leukocyte subpopulations (C) in cows from either control (C) or select (S) genetic groups. Data represents values recorded over the whole 8 month study period.</p
Previous work by our group has consistently identified relationships between immuneassociated (IA... more Previous work by our group has consistently identified relationships between immuneassociated (IA) traits and dairy cow health, fertility and productivity. Furthermore, we observed that these associations are partly under genetic control. SNP genotype data relating to 548 Holstein-Friesian dairy cows were used to carry out a genome-wide association study of IA traits previously linked to production, health and fertility. In this study, we discovered 96 SNPs that were significantly associated with defined IA traits, most notably SNP on chromosome 17 and natural antibodies in a region previously linked with ptosis, intellectual disability, retarded growth and mortality (PIRM) syndrome in cattle. Moreover, 22 SNP were found to be common across 2 to 3 IA traits. A functional cluster analysis was carried out on significant SNPs and showed 9 clusters of genes linked to various immune response and inflammation genes as well as significant associations with biological pathways such as syste...
Samples from bovine viral diarrhoea virus (BVDV)-positive cattle were gathered by Scottish diagno... more Samples from bovine viral diarrhoea virus (BVDV)-positive cattle were gathered by Scottish diagnostic laboratories and used to produce a Biobank of samples with associated location and identification data in support of the Scottish BVDV eradication scheme. The samples were subject to direct amplification and sequencing of the 5'-untranslated region (5'-UTR) to define the viral types and subtypes present. From 2693 samples collected prior to 2016, approximately 2300 sequences were obtained, representing 8 BVDV type 1 subtypes. No BVDV type 2 samples were detected. The samples came from all regions of the UK but 66 per cent were from Scotland. Analysis of the sequences showed great diversity in the 5'-UTR, with 1206 different sequences. Many samples carried virus with identical 5'-UTR sequences; often from single locations, but there were also examples of the same sequence being obtained from samples at several different locations. This work provides a resource that ca...
ABSTRACT The BoLA (bovine lymphocyte antigen) Nomenclature Committee met during the 1994 and 1996... more ABSTRACT The BoLA (bovine lymphocyte antigen) Nomenclature Committee met during the 1994 and 1996 conferences of the International Society for Animal Genetics to define a sequence-based nomenclature system for genes of the BoLA system. The rules for acceptance of new sequences are described and names are assigned to the sequenced alleles of the class II genes DRA, DRB1, DRB2, DRB3, DQA, DQB, DYA, DIB, DMA and DMB. The assignment of BoLA class I sequences to loci will be considered at a later workshop when further sequencing/mapping data are available.
A vector is described for the expression of genomic or cDNA copies of bovine major histocompatibi... more A vector is described for the expression of genomic or cDNA copies of bovine major histocompatibility complex (MHC) class I genes in transfected mouse Ltk- cells. Class I gene fragments are amplified by the polymerase chain reaction, using primers in conserved parts of exon 2 and the 3&#39;-untranslated region of the gene. Amplified class I gene fragments can then be subcloned into the expression vector, pBoLA-21, which contains the necessary 5&#39;- and 3&#39;-sequences for correct expression. The vector was tested by subcloning and expressing genomic and cDNA clones.
The aim of this study was to stimulate immunity in the oro-nasal-pharyngeal region of cattle to p... more The aim of this study was to stimulate immunity in the oro-nasal-pharyngeal region of cattle to protect them from alcelaphine herpesvirus-1 (AlHV-1)-induced malignant catarrhal fever. Attenuated C500 strain AlHV-1 was used along with Freund&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s adjuvant intramuscularly (IM) in the upper neck region to immunise cattle. Virulent C500 strain AlHV-1 was used for intranasal challenge. Nine of ten cattle were protected. Protection was associated with high levels of neutralising antibody in nasal secretions. Some protected animals showed transient low levels of viral DNA in blood samples and in one lymph node sample after challenge whereas viral DNA was detected in the blood and in lymph node samples of all animals with MCF. This is the most promising immunisation strategy to date for the control of malignant catarrhal fever.
<p>Differential leukocyte counts (A), CD4:CD8 ratios (B) and PBMC leukocyte subpopulations ... more <p>Differential leukocyte counts (A), CD4:CD8 ratios (B) and PBMC leukocyte subpopulations (C) in cows from either control (C) or select (S) genetic groups. Data represents values recorded over the whole 8 month study period.</p
Previous work by our group has consistently identified relationships between immuneassociated (IA... more Previous work by our group has consistently identified relationships between immuneassociated (IA) traits and dairy cow health, fertility and productivity. Furthermore, we observed that these associations are partly under genetic control. SNP genotype data relating to 548 Holstein-Friesian dairy cows were used to carry out a genome-wide association study of IA traits previously linked to production, health and fertility. In this study, we discovered 96 SNPs that were significantly associated with defined IA traits, most notably SNP on chromosome 17 and natural antibodies in a region previously linked with ptosis, intellectual disability, retarded growth and mortality (PIRM) syndrome in cattle. Moreover, 22 SNP were found to be common across 2 to 3 IA traits. A functional cluster analysis was carried out on significant SNPs and showed 9 clusters of genes linked to various immune response and inflammation genes as well as significant associations with biological pathways such as syste...
Samples from bovine viral diarrhoea virus (BVDV)-positive cattle were gathered by Scottish diagno... more Samples from bovine viral diarrhoea virus (BVDV)-positive cattle were gathered by Scottish diagnostic laboratories and used to produce a Biobank of samples with associated location and identification data in support of the Scottish BVDV eradication scheme. The samples were subject to direct amplification and sequencing of the 5'-untranslated region (5'-UTR) to define the viral types and subtypes present. From 2693 samples collected prior to 2016, approximately 2300 sequences were obtained, representing 8 BVDV type 1 subtypes. No BVDV type 2 samples were detected. The samples came from all regions of the UK but 66 per cent were from Scotland. Analysis of the sequences showed great diversity in the 5'-UTR, with 1206 different sequences. Many samples carried virus with identical 5'-UTR sequences; often from single locations, but there were also examples of the same sequence being obtained from samples at several different locations. This work provides a resource that ca...
ABSTRACT The BoLA (bovine lymphocyte antigen) Nomenclature Committee met during the 1994 and 1996... more ABSTRACT The BoLA (bovine lymphocyte antigen) Nomenclature Committee met during the 1994 and 1996 conferences of the International Society for Animal Genetics to define a sequence-based nomenclature system for genes of the BoLA system. The rules for acceptance of new sequences are described and names are assigned to the sequenced alleles of the class II genes DRA, DRB1, DRB2, DRB3, DQA, DQB, DYA, DIB, DMA and DMB. The assignment of BoLA class I sequences to loci will be considered at a later workshop when further sequencing/mapping data are available.
A vector is described for the expression of genomic or cDNA copies of bovine major histocompatibi... more A vector is described for the expression of genomic or cDNA copies of bovine major histocompatibility complex (MHC) class I genes in transfected mouse Ltk- cells. Class I gene fragments are amplified by the polymerase chain reaction, using primers in conserved parts of exon 2 and the 3&#39;-untranslated region of the gene. Amplified class I gene fragments can then be subcloned into the expression vector, pBoLA-21, which contains the necessary 5&#39;- and 3&#39;-sequences for correct expression. The vector was tested by subcloning and expressing genomic and cDNA clones.
The aim of this study was to stimulate immunity in the oro-nasal-pharyngeal region of cattle to p... more The aim of this study was to stimulate immunity in the oro-nasal-pharyngeal region of cattle to protect them from alcelaphine herpesvirus-1 (AlHV-1)-induced malignant catarrhal fever. Attenuated C500 strain AlHV-1 was used along with Freund&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s adjuvant intramuscularly (IM) in the upper neck region to immunise cattle. Virulent C500 strain AlHV-1 was used for intranasal challenge. Nine of ten cattle were protected. Protection was associated with high levels of neutralising antibody in nasal secretions. Some protected animals showed transient low levels of viral DNA in blood samples and in one lymph node sample after challenge whereas viral DNA was detected in the blood and in lymph node samples of all animals with MCF. This is the most promising immunisation strategy to date for the control of malignant catarrhal fever.
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