Laser fragmentation in liquids (LFL) allows the synthesis of fully inorganic, ultrasmall gold nan... more Laser fragmentation in liquids (LFL) allows the synthesis of fully inorganic, ultrasmall gold nanoparticles, usAu NPs (<3 nm). Although the general method is well established, there is a lack of un...
In solution, nanoparticles may be conceptually compartmentalized into cores and engineered surfac... more In solution, nanoparticles may be conceptually compartmentalized into cores and engineered surface coatings. Recent advances allow for simple and accurate characterization of nanoparticle cores and surface shells. After introduction into complex biological environment, adsorption of biological molecules to the nanoparticle surface as well as a loss of original surface components occur. Thus, colloidal nanoparticles in the context of biological environment are hybrid materials with complex structure, which may result in different chemical, physical, and biological outcomes as compared to the original engineered nanoparticles. In this perspective, we will discuss building up an engineered inorganic nanoparticle from its inside core to its outside surface, and following its degradation in biological environment from its outside to its inside. This will involve the way to synthesize selected inorganic nanoparticles. Then, we will discuss the environmental changes upon exposure of these nanoparticles to biological media and their uptake by cells. Next, the intracellular fate of nanoparticles and their degradation will be discussed. Based on the examples the need to see nanoparticles in the context of biological environment as dynamic hybrid materials will be highlighted.
Omega-3 essential fatty acids (ω-3 FATs); found in the highest concentrations in fish oil, claim ... more Omega-3 essential fatty acids (ω-3 FATs); found in the highest concentrations in fish oil, claim a plethora of health benefits. The present study aims to evaluate the biological effects of ω-3 FATs supplementation against Ehrlich carcinoma (EC) induced inflammation, oxidative stress, biochemical and histopathological alterations in the liver tissue of albino mouse. ω-3 FATs were orally administered via gavage to mice for a period of 30 consecutive days at a dose of 300 mg/kg body weight. On the 7th day of ω-3 FATs administration, female mice were subcutaneously injected with 0.2 ml of Ehrlich ascite carcinoma for solid tumor induction. The present study revealed that, subcutaneous injection of Ehrlich solid tumor led to hepatic oxidative stress (as significant increase in lipid peroxidation (thiobarbituric acid reactive species, TBARS), concomitant with a significant decrease in glutathione and antioxidant enzymes), systemic inflammation (significant increases in C-reactive protein,...
Laser fragmentation in liquids (LFL) allows the synthesis of fully inorganic, ultrasmall gold nan... more Laser fragmentation in liquids (LFL) allows the synthesis of fully inorganic, ultrasmall gold nanoparticles, usAu NPs (<3 nm). Although the general method is well established, there is a lack of un...
In solution, nanoparticles may be conceptually compartmentalized into cores and engineered surfac... more In solution, nanoparticles may be conceptually compartmentalized into cores and engineered surface coatings. Recent advances allow for simple and accurate characterization of nanoparticle cores and surface shells. After introduction into complex biological environment, adsorption of biological molecules to the nanoparticle surface as well as a loss of original surface components occur. Thus, colloidal nanoparticles in the context of biological environment are hybrid materials with complex structure, which may result in different chemical, physical, and biological outcomes as compared to the original engineered nanoparticles. In this perspective, we will discuss building up an engineered inorganic nanoparticle from its inside core to its outside surface, and following its degradation in biological environment from its outside to its inside. This will involve the way to synthesize selected inorganic nanoparticles. Then, we will discuss the environmental changes upon exposure of these nanoparticles to biological media and their uptake by cells. Next, the intracellular fate of nanoparticles and their degradation will be discussed. Based on the examples the need to see nanoparticles in the context of biological environment as dynamic hybrid materials will be highlighted.
Omega-3 essential fatty acids (ω-3 FATs); found in the highest concentrations in fish oil, claim ... more Omega-3 essential fatty acids (ω-3 FATs); found in the highest concentrations in fish oil, claim a plethora of health benefits. The present study aims to evaluate the biological effects of ω-3 FATs supplementation against Ehrlich carcinoma (EC) induced inflammation, oxidative stress, biochemical and histopathological alterations in the liver tissue of albino mouse. ω-3 FATs were orally administered via gavage to mice for a period of 30 consecutive days at a dose of 300 mg/kg body weight. On the 7th day of ω-3 FATs administration, female mice were subcutaneously injected with 0.2 ml of Ehrlich ascite carcinoma for solid tumor induction. The present study revealed that, subcutaneous injection of Ehrlich solid tumor led to hepatic oxidative stress (as significant increase in lipid peroxidation (thiobarbituric acid reactive species, TBARS), concomitant with a significant decrease in glutathione and antioxidant enzymes), systemic inflammation (significant increases in C-reactive protein,...
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Papers by Mustafa Gharib