... 3. Hampl KF, Heinzmann-Wiedmer S, Luginbuehl I, et al ... JAMES E. ALLISON, MD San Francisco ... more ... 3. Hampl KF, Heinzmann-Wiedmer S, Luginbuehl I, et al ... JAMES E. ALLISON, MD San Francisco General Hospital San Francisco, CA 94110 Editor's note: Dr. Allison has conducted research that has been supported in part by SmithKline Diagnostics (now Beckman Coulter) and ...
The guanine nucleotides GDP, GTP, and guanosine-5'-(beta, gamma-imido)triphosphate inhibi... more The guanine nucleotides GDP, GTP, and guanosine-5'-(beta, gamma-imido)triphosphate inhibit binding of opiates and opioid peptides to receptors solubilized from membranes of neuroblastoma X glioma NG108-15 hybrid cells. The inhibition reflects decreased affinity of receptors for opioid ligands. Whereas in membranes, only opioid agonist binding is sensitive to guanine nucleotide inhibition, both agonist and antagonist binding is reduced in the case of soluble receptors. Furthermore, soluble receptors are more sensitive to the effects of guanine nucleotides than are membrane-bound receptors. These observations are consistent with the suggestion that solubilized receptors may be complexes of an opiate binding protein and a guanine nucleotide-sensitive regulatory component.
... 3. Hampl KF, Heinzmann-Wiedmer S, Luginbuehl I, et al ... JAMES E. ALLISON, MD San Francisco ... more ... 3. Hampl KF, Heinzmann-Wiedmer S, Luginbuehl I, et al ... JAMES E. ALLISON, MD San Francisco General Hospital San Francisco, CA 94110 Editor's note: Dr. Allison has conducted research that has been supported in part by SmithKline Diagnostics (now Beckman Coulter) and ...
The guanine nucleotides GDP, GTP, and guanosine-5'-(beta, gamma-imido)triphosphate inhibi... more The guanine nucleotides GDP, GTP, and guanosine-5'-(beta, gamma-imido)triphosphate inhibit binding of opiates and opioid peptides to receptors solubilized from membranes of neuroblastoma X glioma NG108-15 hybrid cells. The inhibition reflects decreased affinity of receptors for opioid ligands. Whereas in membranes, only opioid agonist binding is sensitive to guanine nucleotide inhibition, both agonist and antagonist binding is reduced in the case of soluble receptors. Furthermore, soluble receptors are more sensitive to the effects of guanine nucleotides than are membrane-bound receptors. These observations are consistent with the suggestion that solubilized receptors may be complexes of an opiate binding protein and a guanine nucleotide-sensitive regulatory component.
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