Avian coccidiosis causes inflammation and decreases growth performance in poultry. Arginine allev... more Avian coccidiosis causes inflammation and decreases growth performance in poultry. Arginine alleviates inflammation and improves growth performance. This study aimed to evaluate the effects of different levels of arginine on growth performance, lipid profile, and inflammatory responses in broiler chicks challenged with Eimeria spp. A total of 384 one-d-old broiler chicks were assigned into eight groups and six replications per group. At 21 days of age, a mixture of Eimeria species was used for induction of challenge in the broiler chicks. Broiler chicks were divided into infected and uninfected groups and received arginine at 85, 100, 125, and 150 % of recommended level. Growth performance, serum nitric oxide (NO), pro-inflammatory cytokines, lipid profile, and faecal oocyst were further assessed. Broiler chicks challenged with coccidiosis showed lower growth performance compared to non-challenged broiler chicks in grower and finisher periods; however, those fed diets containing 125 and 150 % arginine showed better growth performance compared to broiler chicks fed with 85 and100 % levels. The challenged broiler chicks showed higher concentrations for serum NO, lipid profile, and pro-inflammatory cytokines in comparison to uninfected ones. Nevertheless, adding arginine into diet at 125 and 150 % levels increased the levels of serum NO and decreased the concentration of pro-inflammatory cytokines. In conclusion, higher levels of arginine caused anti-inflammatory effects in broiler chicks challenged with coccidiosis.
Exercise training and medicinal herb supplementation may improve microRNAs (miRNAs) expression as... more Exercise training and medicinal herb supplementation may improve microRNAs (miRNAs) expression associated with obesity. This study aimed to assess the effects of 10 weeks of aerobic training (AT) and dill extract (DE) on miR-33 and miR-223 expression of liver in high-fat diet (HFD)-induced obese rats. Forty male Wistar rats were fed a defined high-fat (n = 32) and standard (n = 8, nonobese control [NC]) diet. After obesity induction, obese rats were randomly allocated to four groups: AT, DE, AT + DE, and obese control (OC). Rats were euthanized and plasma and liver tissue samples were collected after the intervention. The liver expression of miR-33 was lower in the AT, DE, AT + DE, and NC groups compared with the OC group. Also, the liver miR-223 expression was higher in the AT, DE, AT + DE, and NC groups compared with the OC group. Moreover, the liver expression of miR-223 in the AT + DE group was higher compared with the AT and DE groups. The AT, DE, AT + DE, and NC groups had lower liver TC compared with the OC group. Also, the plasma level of apolipoprotein B (Apo B) was significantly lower, and liver HDL-C was significantly higher in the AT + DE and NC groups compared with the OC group. These findings show that long-term AT combined with the intake of DE may improve the plasma levels of Apo B, and TC and HDL-C levels in the liver, which is probably due to AT and DE positive effects on miR-33 and miR-223 in the liver of obese rats. PRACTICAL APPLICATIONS: Aerobic training reduces overweight and obesity health problems, however, the duration and intensity of the exercise training distinguish between individuals. We used an integrated approach combining pharmacological and non-pharmacological as a medical strategy to prevent HFD-induced metabolic injury in obese rats. The present results discovered that a combination of AT + DE intervention improves the miR-33 and miR-223 in the liver of obese rats.
Middle East Journal of Rehabilitation and Health Studies
Background: Exercise training through various mechanisms leads to correct telomere homeostasis. T... more Background: Exercise training through various mechanisms leads to correct telomere homeostasis. The potential role of high-intensity interval training (HIIT) to keep up the telomere length has not been recognized in skeletal muscle. Objectives: This study investigated the influence of eight weeks of HIIT with short-term interval (HIITSh) and long-term interval (HIITL), and four weeks of detraining on the regulation of some pathways maintaining telomere length in skeletal muscle. Methods: Fifty-four male Wistar rats were randomly assigned into HIITSh, HIITL, and control groups. Rats were sacrificed after the end of the intervention (eight weeks of training and four weeks of detraining). Gastrocnemius muscle was collected, and telomerase activity, p53 protein, total oxidative status (TOS), and total antioxidant capacity (TAC) levels were measured. Results: We found no change in telomerase activity, p53, TOS, and TAC levels in both HIIT groups compared to the control group (P > 0.05...
Camel milk (CM) has been found to have several health benefits, including antiviral, antibacteria... more Camel milk (CM) has been found to have several health benefits, including antiviral, antibacterial, anti-tumor, anti-fungal, antioxidant, hypoglycaemic and anti-cancer activities. In addition, CM can counter signs of aging and may be a useful naturopathic treatment for autoimmune diseases. The composition of CM varies with geographic origin, feeding conditions, seasonal and physiological changes, genetics and camel health status. In the present review, we collate the diverse scientific literature studying antioxidant, anti-inflammatory and immunomodulatory effects of CM and its bioactive compounds. The databases Scopus, PubMed, and Web of Science were searched until the end of September 2021 using the keywords: camel milk, antioxidant, anti-inflammatory, immunomodulatory. The anti-inflammatory mechanism of CM in various inflammatory disorders was consistently reported to be through modulating inflammatory cells and mediators. The common anti-inflammatory bioactive components of CM s...
Journal of Birjand University of Medical Sciences, 2021
Background and Aims: Researchers have recently considered the impact of herbal supplements combin... more Background and Aims: Researchers have recently considered the impact of herbal supplements combined with exercise on weight control. This study aimed to investigate the effect of Spirulina platensis green-blue algae consumption, and circuit resistance training (CRT) on lipid profile in overweight and obese middle-aged men. Materials and Methods: This was a single-blind, quasi-experimental, and applied study. The study population included 60 overweight and obese men in the age range of 30-55 years with a body mass index of more than 25 (kg/m2). The participants were purposefully selected and randomly divided into four groups of training plus spirulina (N=15); training plus placebo; spirulina; and Placebo. The intervention and placebo groups took two 500 mg spirulina capsules, and placebo capsules daily for eight weeks, respectively. The CRT was performed for eight weeks and three sessions per week based on a flexible timing pattern, with the observation of the principle of overload w...
Background Toll-like receptors (TLRs) are present on monocytes and alveolar macrophages that form... more Background Toll-like receptors (TLRs) are present on monocytes and alveolar macrophages that form the first line of defense against inhaled particles. The importance of those cells in the pathophysiology of chronic obstructive pulmonary disease (COPD) has well been documented. Cigarette smoke contains high concentration of oxidants which can stimulate immune cells to produce reactive oxygen species, cytokines and chemokines. Methods In this study, we evaluated the effects of cigarette smoke medium (CSM) on TLR4 expression and interleukin (IL)-8 production by human macrophages investigating the involvement of ROS. Results and Discussion TLR4 surface expression was downregulated on short term exposure (1 h) of CSM. The downregulation could be explained by internalization of the TLR4 and the upregulation by an increase in TLR4 mRNA. IL-8 mRNA and protein were also increased by CSM. CSM stimulation increased intracellular ROS-production and decreased glutathione (GSH) levels. The modula...
The major risk factor for the development of COPD is cigarette smoking. Smoking causes activation... more The major risk factor for the development of COPD is cigarette smoking. Smoking causes activation of resident cells and the recruitment of inflammatory cells into the lungs, which leads to release of pro-inflammatory cytokines, chemotactic factors, oxygen radicals and proteases. In the present study evidence is found for a new cellular mechanism that refers to a link between smoking and inflammation in lungs. Employing human monocyte-derived macrophages, different techniques including FACS analysis, Cytometric Bead Array Assay and ELISA were achieved to evaluate the effects of CS on pro-inflammatory cytokine secretion including IL-8. Then, Toll-like receptor neutralization was performed to study the involvement of Toll-like receptor-4 in IL-8 production. Finally, signaling pathways in macrophages after exposure to CS medium were investigated performing ELISA and Western analysis. We demonstrate that especially human monocytes are sensitive to produce IL-8 upon cigarette smoke stimul...
Avian coccidiosis causes inflammation and decreases growth performance in poultry. Arginine allev... more Avian coccidiosis causes inflammation and decreases growth performance in poultry. Arginine alleviates inflammation and improves growth performance. This study aimed to evaluate the effects of different levels of arginine on growth performance, lipid profile, and inflammatory responses in broiler chicks challenged with Eimeria spp. A total of 384 one-d-old broiler chicks were assigned into eight groups and six replications per group. At 21 days of age, a mixture of Eimeria species was used for induction of challenge in the broiler chicks. Broiler chicks were divided into infected and uninfected groups and received arginine at 85, 100, 125, and 150 % of recommended level. Growth performance, serum nitric oxide (NO), pro-inflammatory cytokines, lipid profile, and faecal oocyst were further assessed. Broiler chicks challenged with coccidiosis showed lower growth performance compared to non-challenged broiler chicks in grower and finisher periods; however, those fed diets containing 125 and 150 % arginine showed better growth performance compared to broiler chicks fed with 85 and100 % levels. The challenged broiler chicks showed higher concentrations for serum NO, lipid profile, and pro-inflammatory cytokines in comparison to uninfected ones. Nevertheless, adding arginine into diet at 125 and 150 % levels increased the levels of serum NO and decreased the concentration of pro-inflammatory cytokines. In conclusion, higher levels of arginine caused anti-inflammatory effects in broiler chicks challenged with coccidiosis.
Exercise training and medicinal herb supplementation may improve microRNAs (miRNAs) expression as... more Exercise training and medicinal herb supplementation may improve microRNAs (miRNAs) expression associated with obesity. This study aimed to assess the effects of 10 weeks of aerobic training (AT) and dill extract (DE) on miR-33 and miR-223 expression of liver in high-fat diet (HFD)-induced obese rats. Forty male Wistar rats were fed a defined high-fat (n = 32) and standard (n = 8, nonobese control [NC]) diet. After obesity induction, obese rats were randomly allocated to four groups: AT, DE, AT + DE, and obese control (OC). Rats were euthanized and plasma and liver tissue samples were collected after the intervention. The liver expression of miR-33 was lower in the AT, DE, AT + DE, and NC groups compared with the OC group. Also, the liver miR-223 expression was higher in the AT, DE, AT + DE, and NC groups compared with the OC group. Moreover, the liver expression of miR-223 in the AT + DE group was higher compared with the AT and DE groups. The AT, DE, AT + DE, and NC groups had lower liver TC compared with the OC group. Also, the plasma level of apolipoprotein B (Apo B) was significantly lower, and liver HDL-C was significantly higher in the AT + DE and NC groups compared with the OC group. These findings show that long-term AT combined with the intake of DE may improve the plasma levels of Apo B, and TC and HDL-C levels in the liver, which is probably due to AT and DE positive effects on miR-33 and miR-223 in the liver of obese rats. PRACTICAL APPLICATIONS: Aerobic training reduces overweight and obesity health problems, however, the duration and intensity of the exercise training distinguish between individuals. We used an integrated approach combining pharmacological and non-pharmacological as a medical strategy to prevent HFD-induced metabolic injury in obese rats. The present results discovered that a combination of AT + DE intervention improves the miR-33 and miR-223 in the liver of obese rats.
Middle East Journal of Rehabilitation and Health Studies
Background: Exercise training through various mechanisms leads to correct telomere homeostasis. T... more Background: Exercise training through various mechanisms leads to correct telomere homeostasis. The potential role of high-intensity interval training (HIIT) to keep up the telomere length has not been recognized in skeletal muscle. Objectives: This study investigated the influence of eight weeks of HIIT with short-term interval (HIITSh) and long-term interval (HIITL), and four weeks of detraining on the regulation of some pathways maintaining telomere length in skeletal muscle. Methods: Fifty-four male Wistar rats were randomly assigned into HIITSh, HIITL, and control groups. Rats were sacrificed after the end of the intervention (eight weeks of training and four weeks of detraining). Gastrocnemius muscle was collected, and telomerase activity, p53 protein, total oxidative status (TOS), and total antioxidant capacity (TAC) levels were measured. Results: We found no change in telomerase activity, p53, TOS, and TAC levels in both HIIT groups compared to the control group (P > 0.05...
Camel milk (CM) has been found to have several health benefits, including antiviral, antibacteria... more Camel milk (CM) has been found to have several health benefits, including antiviral, antibacterial, anti-tumor, anti-fungal, antioxidant, hypoglycaemic and anti-cancer activities. In addition, CM can counter signs of aging and may be a useful naturopathic treatment for autoimmune diseases. The composition of CM varies with geographic origin, feeding conditions, seasonal and physiological changes, genetics and camel health status. In the present review, we collate the diverse scientific literature studying antioxidant, anti-inflammatory and immunomodulatory effects of CM and its bioactive compounds. The databases Scopus, PubMed, and Web of Science were searched until the end of September 2021 using the keywords: camel milk, antioxidant, anti-inflammatory, immunomodulatory. The anti-inflammatory mechanism of CM in various inflammatory disorders was consistently reported to be through modulating inflammatory cells and mediators. The common anti-inflammatory bioactive components of CM s...
Journal of Birjand University of Medical Sciences, 2021
Background and Aims: Researchers have recently considered the impact of herbal supplements combin... more Background and Aims: Researchers have recently considered the impact of herbal supplements combined with exercise on weight control. This study aimed to investigate the effect of Spirulina platensis green-blue algae consumption, and circuit resistance training (CRT) on lipid profile in overweight and obese middle-aged men. Materials and Methods: This was a single-blind, quasi-experimental, and applied study. The study population included 60 overweight and obese men in the age range of 30-55 years with a body mass index of more than 25 (kg/m2). The participants were purposefully selected and randomly divided into four groups of training plus spirulina (N=15); training plus placebo; spirulina; and Placebo. The intervention and placebo groups took two 500 mg spirulina capsules, and placebo capsules daily for eight weeks, respectively. The CRT was performed for eight weeks and three sessions per week based on a flexible timing pattern, with the observation of the principle of overload w...
Background Toll-like receptors (TLRs) are present on monocytes and alveolar macrophages that form... more Background Toll-like receptors (TLRs) are present on monocytes and alveolar macrophages that form the first line of defense against inhaled particles. The importance of those cells in the pathophysiology of chronic obstructive pulmonary disease (COPD) has well been documented. Cigarette smoke contains high concentration of oxidants which can stimulate immune cells to produce reactive oxygen species, cytokines and chemokines. Methods In this study, we evaluated the effects of cigarette smoke medium (CSM) on TLR4 expression and interleukin (IL)-8 production by human macrophages investigating the involvement of ROS. Results and Discussion TLR4 surface expression was downregulated on short term exposure (1 h) of CSM. The downregulation could be explained by internalization of the TLR4 and the upregulation by an increase in TLR4 mRNA. IL-8 mRNA and protein were also increased by CSM. CSM stimulation increased intracellular ROS-production and decreased glutathione (GSH) levels. The modula...
The major risk factor for the development of COPD is cigarette smoking. Smoking causes activation... more The major risk factor for the development of COPD is cigarette smoking. Smoking causes activation of resident cells and the recruitment of inflammatory cells into the lungs, which leads to release of pro-inflammatory cytokines, chemotactic factors, oxygen radicals and proteases. In the present study evidence is found for a new cellular mechanism that refers to a link between smoking and inflammation in lungs. Employing human monocyte-derived macrophages, different techniques including FACS analysis, Cytometric Bead Array Assay and ELISA were achieved to evaluate the effects of CS on pro-inflammatory cytokine secretion including IL-8. Then, Toll-like receptor neutralization was performed to study the involvement of Toll-like receptor-4 in IL-8 production. Finally, signaling pathways in macrophages after exposure to CS medium were investigated performing ELISA and Western analysis. We demonstrate that especially human monocytes are sensitive to produce IL-8 upon cigarette smoke stimul...
Uploads
Papers by Hadi Sarir