The aim of this study was to evaluate, for the first time, the antidiabetic effect of the alkaloi... more The aim of this study was to evaluate, for the first time, the antidiabetic effect of the alkaloids extract of Salvia chudaei Batt. & Trab. (Lamiaceae) on alloxan-induced diabetic rats. The alkaloids extract was prepared, and the in vitro inhibitory effect of key digesting enzymes related to postprandial hyperglycemia were determined. After acute toxicity test, the Swiss albino rats were induced with alloxan to get experimental diabetes animals. The fasting mean blood glucose, lipid profile, different liver and kidney function biomarkers and antioxidant biomarkers levels, after treatment for 30 days, diabetic untreated and diabetic rats treated with alkaloids extract were estimated. The alkaloids displayed remarkable in inhibiting ?-glucosidase (IC50 = 248.25?2.61 ?g/ml) than ?-amylase (IC50 = 262.96?9.64 ?g/ml) activities. In vivo, the results proved that alkaloids extract at dose of 500 mg/kg bw decreased significantly the blood glucose, lipid profile levels and improved the liver...
Leuconostoc (Ln) sp. belongs to a group of lactic acid bacteria, which has the capacity to produc... more Leuconostoc (Ln) sp. belongs to a group of lactic acid bacteria, which has the capacity to produce dextran (an exopolysaccharides) in the presence of su-crose. dextran is industrially important, it was the first microbial exopolysac-charide affirmed for commercial use. This study aimed to optimize the pro-duction of the synthesized dextran by Ln strains species isolated from differ-ent dairy products. Morphological, cultural, physiological and biochemical characteristics were employed to identify 23 isolated strains. We have identi-fied the species: Ln. gelidum, Ln. carnosum, Ln. citreum, Ln. fallax, Ln. mesen-teroides subsp mesenteroides, Ln. mesenteroides subsp dextranicum, Ln. mesenteroides subsp cremoris. 20 strains had the capacity to produce dex-tran from sucrose. The precipitation and quantification of EPS on MRSs (Mark rogosa et sharpe sucrose) medium revealed a difference between the strains, by the total sugars assay method, the amount of EPS varied between 0.63 ± 0.19 and...
The antioxidants are the subject of many studies, because in addition to their use as preservativ... more The antioxidants are the subject of many studies, because in addition to their use as preservatives in food products by replacing the synthetic antioxidants, they intervene in the treatment of many diseases. In this study, we evaluated the antioxidant properties of leaves of atriplex halimus L as well as flowers and stems of haloxylon scoparium pomel. Butanolic Fractions of flowers of the two plants have a higher total phenols content; the fraction of ethyl acetate and butanol, for these two plants, presents a better total antioxidant capacity, and higher activity to trap the DPPH radical, radical OH and the ABTS radical cation.
Une enquete aupres de la population autochtone de la region de Ouargla a permis d’inventorier 37... more Une enquete aupres de la population autochtone de la region de Ouargla a permis d’inventorier 37 especes; 27 ont pu etre echantillonnees sur terrain. Les familles les plus importantes sont les Asteracees (13,51%), les Poacees (10,81%), les Chenopodiacees (8,10 %) et les Labiees (8,10%). Les maladies dominantes sont la pathologie digestive (26,38%), les algies diverses (15,27), les dermatoses (13,88 %), la pathologie broncho-pulmonaire avec 12,50% chacune, les pathologies feminines et les piqures de scorpion, avec 9,72 % chacune. L’administration orale, qui regroupe la majorite des modes de preparation: infusion, maceration, decoction, poudre interne, est la plus preconisee. Les parties utilisees sont respectivement les feuilles, les tiges, les fruits, les racines et les inflorescences.
This work presents a contribution to the detection of biological activity of some secondary metab... more This work presents a contribution to the detection of biological activity of some secondary metabolites of Teucrium polium geyrii, which is a Lamiaceae plant family of Tamanrasset (southern Algeria). The plant was subjected to two types of phenolic compounds extraction, maceration and Soxhlet apparatus. The semi-quantitative analysis of total phenols and flavonoids (phenolic extracts), was performed by colorimetry. It showed that the ethyl acetate extract obtained by maceration contains the largest amount of total phenols, 89.05±0.50 mg/g equivalent of gallic acid, as well as the rate of flavonoids with 0.45±0.001 mg/g equivalent of vitexin. The antibacterial activity carried on bacterial strains pathogenic and alterations, Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus cremoris, Clostridium perfringens, Klebsilla pneumoniae, Escherichia coli and Proteus mirabilis, by Aromatogram method, showed that the extracts tested are endowed interesting antibacterial activity wit...
One of the major environmental problems today is hydrocarbon contamination resulting from the act... more One of the major environmental problems today is hydrocarbon contamination resulting from the activities related to the petrochemical industry. Accidental releases of petroleum products in the environment are of particular concern. Hydrocarbon components have been known to belong to the family of carcinogens and neurotoxic organic pollutants. The present work aims to implement a system to reduce or even eliminate hydrocarbons; it consists in columns composed of a fixed bed of sand dunes of N’Goussa from the Region of Ouargla (Algerian South). Columns gave excellent results regarding the removal efficiency of hydrocarbons, since a yield of up to 99.65 % was obtained.
UNIVERSITE KASDI MERBAH - OUARGLA Faculte des Sciences de la Nature et de la Vie Departement des ... more UNIVERSITE KASDI MERBAH - OUARGLA Faculte des Sciences de la Nature et de la Vie Departement des Sciences Biologiques
The present research focuses on the biological activities study of the essential oils and extract... more The present research focuses on the biological activities study of the essential oils and extracts preparations using organic solvents of different polarity from Salvia chudaei Batt. & Trab. (Lamiaceae) plant, wild growing in Algeria. The essential oils and solvent extracts were tested in vitro against two reference clones of Plasmodium falciparum: the K1 chIoroquine-resistant and the 3d7 chloroquine-sensitive strains. A significant inhibitory activity was observed with IC50 values between (IC50=2.39 μg.mL-1 and 7.99 ± 1.06 μg.mL-1). The antimicrobial activity of the essential oil and the others organic extracts was evaluated against Staphylococcus aureus ATCC 27923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 25853 and Candida albicans using disc diffusion method. The essential oil showed an efficient antibacterial activity against Staphylococcus aureus with the strongest inhibition zone (26 mm). The minimal inhibitory activity (MIC) ranged from 0.019 to 37.92 μg.mL-1....
The chemical compositions of the volatile fractions, produced by the flowers and leaves of two Ol... more The chemical compositions of the volatile fractions, produced by the flowers and leaves of two Olea europaea L. cultivars (Chemlal and Sigoise) were investigated. The plant materials were harvested from two different zones in Algeria (Mediterranean and arid regions). The samples were extracted by hydrodistillation using a Clevenger apparatus. GC-MS analysis allowed the identification of 51 compounds. According to the geographic origin and the cultivar, qualitative and semi-quantitative variations were observed, especially with the abundance of the main compounds. Overall, the principal component analysis showed that the environmental condition was mainly affecting the production of the major compounds. Throughout the two regions, the main substances in the samples from the Mediterranean region were (Z)-jasmone (51.8%), nonanal (32.4%), theaspirane II (23.7%) and phenylethyl propionate (17.9%). While those of the arid region were bornyl acetate (26.8%), 3-ethenyl pyridine (17.2%), (E...
During this work, the polyphenols in both Asteraceae Brocchia cinerea (Vis.) and Matricaria pubes... more During this work, the polyphenols in both Asteraceae Brocchia cinerea (Vis.) and Matricaria pubescens (Desf.) were targeted by two extraction methods, using different solvents. The extracts obtained by ethyl acetate were selected for column fractionation. Indeed, four fractions are obtained for M. pubescens and five for B. cinerea. The evaluation of the antioxidant capacity of these samples was carried out by two tests, commonly used in this field. The first one uses the free radical 1, 1-diphenyl-2 - picrylhydrazyl (DPPH) while the other (FRAP) uses 2,4,6-tris-2- pyridyl-s-triazine (TPTZ). These two tests revealed the radical scavenging power and reducing potential of both species B. cinerea and M. pubescens. Results have shown that the fraction obtained with the mixture EtOH/petroleum ether at (60/40) in the case of B. cinerea and that trained by 75% methanol in dichloromethane from M. pubescens, contained the majority of their active compositions. AcOEt extracts of both species a...
The extraction of essential oils from Marrubium deserti leaves has been carried out using the wat... more The extraction of essential oils from Marrubium deserti leaves has been carried out using the water steam practice. The analysis of the essential oils by GC-MS, showed that 27 components representing 62,91% of the oil and mainly belonging to the class of sesquiterpenes. The major constituents were δ-cadinene (17,63%), germacrene D (7,01%) trimethylpentadeca-2-one (4,61%) and 9-methyl-undecene (4,17%). The results were showed the existence of diverse particular structures that have been detected for the first time in Marrubium species. In another hand several abundant alkanes belonging to the odd-numbered carbon atom series have been identified.
During this work, the polyphenols in both Asteraceae Brocchia cinerea (Vis.) and Matricaria pubes... more During this work, the polyphenols in both Asteraceae Brocchia cinerea (Vis.) and Matricaria pubescens (Desf.) were targeted by two extraction methods, using different solvents. The extracts obtained by ethyl acetate were selected for column fractionation. Indeed, four fractions are obtained for M. pubescens and five for B. cinerea. The evaluation of the antioxidant capacity of these samples was carried out by two tests, commonly used in this field. The first one uses the free radical 1, 1-diphenyl-2 - picrylhydrazyl (DPPH) while the other (FRAP) uses 2,4,6-tris-2- pyridyl-s-triazine (TPTZ). These two tests revealed the radical scavenging power and reducing potential of both species B. cinerea and M. pubescens. Results have shown that the fraction obtained with the mixture EtOH/petroleum ether at (60/40) in the case of B. cinerea and that trained by 75% methanol in dichloromethane from M. pubescens, contained the majority of their active compositions. AcOEt extracts of both species a...
The aim of this study was to evaluate, for the first time, the antidiabetic effect of the alkaloi... more The aim of this study was to evaluate, for the first time, the antidiabetic effect of the alkaloids extract of Salvia chudaei Batt. & Trab. (Lamiaceae) on alloxan-induced diabetic rats. The alkaloids extract was prepared, and the in vitro inhibitory effect of key digesting enzymes related to postprandial hyperglycemia were determined. After acute toxicity test, the Swiss albino rats were induced with alloxan to get experimental diabetes animals. The fasting mean blood glucose, lipid profile, different liver and kidney function biomarkers and antioxidant biomarkers levels, after treatment for 30 days, diabetic untreated and diabetic rats treated with alkaloids extract were estimated. The alkaloids displayed remarkable in inhibiting ?-glucosidase (IC50 = 248.25?2.61 ?g/ml) than ?-amylase (IC50 = 262.96?9.64 ?g/ml) activities. In vivo, the results proved that alkaloids extract at dose of 500 mg/kg bw decreased significantly the blood glucose, lipid profile levels and improved the liver...
Leuconostoc (Ln) sp. belongs to a group of lactic acid bacteria, which has the capacity to produc... more Leuconostoc (Ln) sp. belongs to a group of lactic acid bacteria, which has the capacity to produce dextran (an exopolysaccharides) in the presence of su-crose. dextran is industrially important, it was the first microbial exopolysac-charide affirmed for commercial use. This study aimed to optimize the pro-duction of the synthesized dextran by Ln strains species isolated from differ-ent dairy products. Morphological, cultural, physiological and biochemical characteristics were employed to identify 23 isolated strains. We have identi-fied the species: Ln. gelidum, Ln. carnosum, Ln. citreum, Ln. fallax, Ln. mesen-teroides subsp mesenteroides, Ln. mesenteroides subsp dextranicum, Ln. mesenteroides subsp cremoris. 20 strains had the capacity to produce dex-tran from sucrose. The precipitation and quantification of EPS on MRSs (Mark rogosa et sharpe sucrose) medium revealed a difference between the strains, by the total sugars assay method, the amount of EPS varied between 0.63 ± 0.19 and...
The antioxidants are the subject of many studies, because in addition to their use as preservativ... more The antioxidants are the subject of many studies, because in addition to their use as preservatives in food products by replacing the synthetic antioxidants, they intervene in the treatment of many diseases. In this study, we evaluated the antioxidant properties of leaves of atriplex halimus L as well as flowers and stems of haloxylon scoparium pomel. Butanolic Fractions of flowers of the two plants have a higher total phenols content; the fraction of ethyl acetate and butanol, for these two plants, presents a better total antioxidant capacity, and higher activity to trap the DPPH radical, radical OH and the ABTS radical cation.
Une enquete aupres de la population autochtone de la region de Ouargla a permis d’inventorier 37... more Une enquete aupres de la population autochtone de la region de Ouargla a permis d’inventorier 37 especes; 27 ont pu etre echantillonnees sur terrain. Les familles les plus importantes sont les Asteracees (13,51%), les Poacees (10,81%), les Chenopodiacees (8,10 %) et les Labiees (8,10%). Les maladies dominantes sont la pathologie digestive (26,38%), les algies diverses (15,27), les dermatoses (13,88 %), la pathologie broncho-pulmonaire avec 12,50% chacune, les pathologies feminines et les piqures de scorpion, avec 9,72 % chacune. L’administration orale, qui regroupe la majorite des modes de preparation: infusion, maceration, decoction, poudre interne, est la plus preconisee. Les parties utilisees sont respectivement les feuilles, les tiges, les fruits, les racines et les inflorescences.
This work presents a contribution to the detection of biological activity of some secondary metab... more This work presents a contribution to the detection of biological activity of some secondary metabolites of Teucrium polium geyrii, which is a Lamiaceae plant family of Tamanrasset (southern Algeria). The plant was subjected to two types of phenolic compounds extraction, maceration and Soxhlet apparatus. The semi-quantitative analysis of total phenols and flavonoids (phenolic extracts), was performed by colorimetry. It showed that the ethyl acetate extract obtained by maceration contains the largest amount of total phenols, 89.05±0.50 mg/g equivalent of gallic acid, as well as the rate of flavonoids with 0.45±0.001 mg/g equivalent of vitexin. The antibacterial activity carried on bacterial strains pathogenic and alterations, Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus cremoris, Clostridium perfringens, Klebsilla pneumoniae, Escherichia coli and Proteus mirabilis, by Aromatogram method, showed that the extracts tested are endowed interesting antibacterial activity wit...
One of the major environmental problems today is hydrocarbon contamination resulting from the act... more One of the major environmental problems today is hydrocarbon contamination resulting from the activities related to the petrochemical industry. Accidental releases of petroleum products in the environment are of particular concern. Hydrocarbon components have been known to belong to the family of carcinogens and neurotoxic organic pollutants. The present work aims to implement a system to reduce or even eliminate hydrocarbons; it consists in columns composed of a fixed bed of sand dunes of N’Goussa from the Region of Ouargla (Algerian South). Columns gave excellent results regarding the removal efficiency of hydrocarbons, since a yield of up to 99.65 % was obtained.
UNIVERSITE KASDI MERBAH - OUARGLA Faculte des Sciences de la Nature et de la Vie Departement des ... more UNIVERSITE KASDI MERBAH - OUARGLA Faculte des Sciences de la Nature et de la Vie Departement des Sciences Biologiques
The present research focuses on the biological activities study of the essential oils and extract... more The present research focuses on the biological activities study of the essential oils and extracts preparations using organic solvents of different polarity from Salvia chudaei Batt. & Trab. (Lamiaceae) plant, wild growing in Algeria. The essential oils and solvent extracts were tested in vitro against two reference clones of Plasmodium falciparum: the K1 chIoroquine-resistant and the 3d7 chloroquine-sensitive strains. A significant inhibitory activity was observed with IC50 values between (IC50=2.39 μg.mL-1 and 7.99 ± 1.06 μg.mL-1). The antimicrobial activity of the essential oil and the others organic extracts was evaluated against Staphylococcus aureus ATCC 27923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 25853 and Candida albicans using disc diffusion method. The essential oil showed an efficient antibacterial activity against Staphylococcus aureus with the strongest inhibition zone (26 mm). The minimal inhibitory activity (MIC) ranged from 0.019 to 37.92 μg.mL-1....
The chemical compositions of the volatile fractions, produced by the flowers and leaves of two Ol... more The chemical compositions of the volatile fractions, produced by the flowers and leaves of two Olea europaea L. cultivars (Chemlal and Sigoise) were investigated. The plant materials were harvested from two different zones in Algeria (Mediterranean and arid regions). The samples were extracted by hydrodistillation using a Clevenger apparatus. GC-MS analysis allowed the identification of 51 compounds. According to the geographic origin and the cultivar, qualitative and semi-quantitative variations were observed, especially with the abundance of the main compounds. Overall, the principal component analysis showed that the environmental condition was mainly affecting the production of the major compounds. Throughout the two regions, the main substances in the samples from the Mediterranean region were (Z)-jasmone (51.8%), nonanal (32.4%), theaspirane II (23.7%) and phenylethyl propionate (17.9%). While those of the arid region were bornyl acetate (26.8%), 3-ethenyl pyridine (17.2%), (E...
During this work, the polyphenols in both Asteraceae Brocchia cinerea (Vis.) and Matricaria pubes... more During this work, the polyphenols in both Asteraceae Brocchia cinerea (Vis.) and Matricaria pubescens (Desf.) were targeted by two extraction methods, using different solvents. The extracts obtained by ethyl acetate were selected for column fractionation. Indeed, four fractions are obtained for M. pubescens and five for B. cinerea. The evaluation of the antioxidant capacity of these samples was carried out by two tests, commonly used in this field. The first one uses the free radical 1, 1-diphenyl-2 - picrylhydrazyl (DPPH) while the other (FRAP) uses 2,4,6-tris-2- pyridyl-s-triazine (TPTZ). These two tests revealed the radical scavenging power and reducing potential of both species B. cinerea and M. pubescens. Results have shown that the fraction obtained with the mixture EtOH/petroleum ether at (60/40) in the case of B. cinerea and that trained by 75% methanol in dichloromethane from M. pubescens, contained the majority of their active compositions. AcOEt extracts of both species a...
The extraction of essential oils from Marrubium deserti leaves has been carried out using the wat... more The extraction of essential oils from Marrubium deserti leaves has been carried out using the water steam practice. The analysis of the essential oils by GC-MS, showed that 27 components representing 62,91% of the oil and mainly belonging to the class of sesquiterpenes. The major constituents were δ-cadinene (17,63%), germacrene D (7,01%) trimethylpentadeca-2-one (4,61%) and 9-methyl-undecene (4,17%). The results were showed the existence of diverse particular structures that have been detected for the first time in Marrubium species. In another hand several abundant alkanes belonging to the odd-numbered carbon atom series have been identified.
During this work, the polyphenols in both Asteraceae Brocchia cinerea (Vis.) and Matricaria pubes... more During this work, the polyphenols in both Asteraceae Brocchia cinerea (Vis.) and Matricaria pubescens (Desf.) were targeted by two extraction methods, using different solvents. The extracts obtained by ethyl acetate were selected for column fractionation. Indeed, four fractions are obtained for M. pubescens and five for B. cinerea. The evaluation of the antioxidant capacity of these samples was carried out by two tests, commonly used in this field. The first one uses the free radical 1, 1-diphenyl-2 - picrylhydrazyl (DPPH) while the other (FRAP) uses 2,4,6-tris-2- pyridyl-s-triazine (TPTZ). These two tests revealed the radical scavenging power and reducing potential of both species B. cinerea and M. pubescens. Results have shown that the fraction obtained with the mixture EtOH/petroleum ether at (60/40) in the case of B. cinerea and that trained by 75% methanol in dichloromethane from M. pubescens, contained the majority of their active compositions. AcOEt extracts of both species a...
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