The purpose of this experimental study is to identify the effectiveness of platelet-rich plasma (... more The purpose of this experimental study is to identify the effectiveness of platelet-rich plasma (PRP) and efficacy of intralesional injection as an application method on acute cutaneous wounds in dogs. Healthy adult beagle dogs (n=3) were used in this study. Autologous PRP was separated from anticoagulant treated whole blood in 3 dogs. Cutaneous wounds were created and treated with intralesional injection of PRP in experimental group while treated with saline in control group, on day 0, 2 and 4. Gross examination during whole period and histologic examination on day 7, 14 and 21 were performed to evaluate healing process. In PRP treated wounds, mean diameter of wounds was smaller and wound closure rate was higher than control. In histological study, PRP treated wounds showed more granulation formation and angiogenesis on day 7, and faster epithelialization, more granulation formation and collagen deposition were observed on day14 than control wounds. On day 21, collagen deposition a...
An experimental study was performed to measure a ice concentration of ice slurry flowing in a pip... more An experimental study was performed to measure a ice concentration of ice slurry flowing in a pipe in a real time. In the present paper, we suggested a measuring method by a refractive index and compared it to other methods by a freezing point and a density. To measure the refractive index of the solution, ice particles in the ice slurry should be completely removed and a hydro-cyclone was introduced instead of a mesh. The measuring method through the refractive index coincided with the density method using the real-time solution density within error range, having the error range less than the other two methods. In the other hand, the measuring method through the density has a good resolution, but the result using the initial density of the solution was different more than 10% error from that using the real-time density. And it has an error range 1.5 times greater than the method through the refractive index.
Wounds : a compendium of clinical research and practice, 2013
Silver plays an important part in severe wound management, mainly by reducing microbial growth wi... more Silver plays an important part in severe wound management, mainly by reducing microbial growth within dressed wounds and wound beds. However, it is unknown how silver-coated dressing materials affect diabetic wounds. The purpose of this study is to evaluate the efficacy of silver-containing dressing materials for the treatment of methicillin-resistant Staphylococcus aureus (MRSA)-infected wounds in streptozotocininduced diabetic rats. Full-thickness skin defects were created on the backs of rats with streptozotocin (STZ)-induced diabetes (n = 108) and were infected with MRSA. The rats were assigned to 6 groups according to the wound dressing used: nanocrystalline silver (Acticoat, Smith & Nephew, Inc, London, UK), silver carboxymethylcellulose (Aquacel-Ag, ConvaTec, Skillman, NJ), silver sulfadiazine (Medifoam silver, Genewel Science Co Ltd, Seongnam, South Korea), nanocrystalline silver (Poly- Mem silver, Ferris Mfg Corp, Burr Ridge, IL), silver sulfadiazine (Ilvadon, Ildong Pharma...
In this study, the use of canine experimental autoimmune encephalomyelitis (EAE) as a disease mod... more In this study, the use of canine experimental autoimmune encephalomyelitis (EAE) as a disease model for necrotizing encephalitis (NE) was assessed. Twelve healthy dogs (1-4 years old; 4.5-13.1 kg; 3 females/9 males; 9 Beagles/1 Schnauzer/2 Mongrels) were used in this study as experimental models. Canine forebrain tissue (8 g), including white and grey matter, were homogenized with 4 mL of phosphate-buffered saline for 5 min in an ice bath. The suspension was emulsified with the same volume of Freund's Complete Adjuvant containing 1 mg/mL of killed Mycobacterium tuberculosis H37Ra. Under sedation, each dog was injected subcutaneously with canine brain homogenate over 4 sites, with 2 in the inguinal and 2 in the axillary regions. A second injection (booster shot) was performed with the same procedure 7 days after the first injection in all dogs. Clinical assessments, magnetic resonance imaging, cerebrospinal fluid analyses, necropsies, and histopathological and immunohistochemical...
Cancer research and treatment : official journal of Korean Cancer Association, Jan 17, 2015
A 50-year-old woman was admitted to our hospital due to multiple lung nodules detected incidental... more A 50-year-old woman was admitted to our hospital due to multiple lung nodules detected incidentally on a chest X-ray. A video-assisted thoracoscopic lung biopsy revealed low-grade endometrial stromal sarcoma (LG-ESS). She had undergone a simple hysterectomy one year earlier owing to a diagnosis of adenomyosis. A review of her previous hysterectomy specimen showed not endometriosis but LG-ESS. According to the patient's levels of serum FSH and estradiol, she was in the pre-menopausal state with retained and normally functioning ovaries. She then underwent ovarian ablation by radiotherapy, after which she was administered 2.5 mg of letrozole once per day. Three months later, the size of the metastatic nodules in both lungs had decreased. The patient was followed up for 24 months while continuing on letrozole, and maintained a partial remission. We report herein on a case of metastatic LG-ESS treated with letrozole after ovarian ablation by radiotherapy.
An 8-year-old intact male Belgian Malinois, weighing 37.2 kg, was referred for evaluation due to ... more An 8-year-old intact male Belgian Malinois, weighing 37.2 kg, was referred for evaluation due to right side facial paresis, ataxia and a 2-month history of decreased cognitive ability. Physical and neurological examinations revealed mild depression, left-sided head tilt, right-sided facial paresis and ataxia. A well-demarcated, broad-based cerebellar mass and hyperostosis were found on CT imaging of the brain. Based on these CT findings, a cerebellar meningioma was strongly suspected. Hydroxyurea and prednisolone were administered; after 4 weeks, there was reduction in mass size as compared to initial CT results. However, the mass size was found to have grown 6 weeks after hydroxyurea treatment. We then prescribed a combination of imatinib mesylate and hydroxyurea. Two weeks following combination treatment, the mass size had reduced significantly. The mass continuously decreased in size until the patient died during anesthesia. Cerebellar transitional meningioma was confirmed by histopathologic examination. To the author's knowledge, this is the first reported case of imatinib mesylate plus hydroxyurea therapy for the treatment of meningioma in veterinary medicine.
ABSTRACT The purpose of this study is to merge clinical information from various neurological pat... more ABSTRACT The purpose of this study is to merge clinical information from various neurological patients of the Gyeongsang National University Animal Medical Center during the study period (from Jan.2008 to Jan.2012). Total 172 cases of neurological disorders were diagnosed and treated in the study period. Evaluated criteria of this study were breed, age, sex, clinical signs, and diagnosis. Physical examination, neurological examination, complete blood count, serum chemistry profile, radiography, ultrasonography, cerebrospinal fluid (CSF) analysis, computed tomography (CT), magnetic resonance imaging (MRI), and electroencephalography (EEG) were performed on the basis of individual cases. Among all of 172 dogs, 78 dogs (45.3%) were diagnosed with meningoencephalitis of unknown etiology (MUE); 38 dogs (22.0%) were intervertebral disk disease (IVDD); 15 dogs (8.7%) were idiopathic epilepsy; 13 dogs (7.5%) were hydrocephalus; 5 dogs (2.9%) were brain tumor; and the remainder was diagnosed with miscellaneous diseases. The canine neurological disease model is very useful to understand human neurological disease because the dog is structurally and functionally similar to the human and have same natural neurological diseases as human based on this study. Thus, we hope that this study could help to develop the canine neurological disease model.
A 4-year-old female Maltese (case 1), a 9-year-old castrated male shih tzu (case 2) and 2-year-ol... more A 4-year-old female Maltese (case 1), a 9-year-old castrated male shih tzu (case 2) and 2-year-old female Pomeranian (case 3) presented with neurological signs, such as head tilt, ataxia, circling and paresis. The three cases were tentatively diagnosed as having meningoencephalitis of unknown etiology based on computed tomography scan and cerebrospinal fluid analysis. All patients were managed with cyclosporine plus prednisolone therapy. The survival times of the three patients were 170, 70 and 21 days, respectively. After the cases died, we performed necropsy and histopathological examination for definitive diagnosis. Based on the necropsy, histopathological and immunohistochemical examinations, cases 1, 2 and 3 were definitely diagnosed as having necrotizing meningoencephalitis, necrotizing leukoencephalitis and granulomatous meningoencephalitis, respectively. This case report demonstrated the clinical findings, brain CT characteristics and histopathological and immunohistochemical features of NME, NLE and GME in dogs and discussed the reason for the relatively short survival times under cyclosporine plus prednisolone therapy.
The purpose of this experimental study is to identify the effectiveness of platelet-rich plasma (... more The purpose of this experimental study is to identify the effectiveness of platelet-rich plasma (PRP) and efficacy of intralesional injection as an application method on acute cutaneous wounds in dogs. Healthy adult beagle dogs (n=3) were used in this study. Autologous PRP was separated from anticoagulant treated whole blood in 3 dogs. Cutaneous wounds were created and treated with intralesional injection of PRP in experimental group while treated with saline in control group, on day 0, 2 and 4. Gross examination during whole period and histologic examination on day 7, 14 and 21 were performed to evaluate healing process. In PRP treated wounds, mean diameter of wounds was smaller and wound closure rate was higher than control. In histological study, PRP treated wounds showed more granulation formation and angiogenesis on day 7, and faster epithelialization, more granulation formation and collagen deposition were observed on day14 than control wounds. On day 21, collagen deposition a...
An experimental study was performed to measure a ice concentration of ice slurry flowing in a pip... more An experimental study was performed to measure a ice concentration of ice slurry flowing in a pipe in a real time. In the present paper, we suggested a measuring method by a refractive index and compared it to other methods by a freezing point and a density. To measure the refractive index of the solution, ice particles in the ice slurry should be completely removed and a hydro-cyclone was introduced instead of a mesh. The measuring method through the refractive index coincided with the density method using the real-time solution density within error range, having the error range less than the other two methods. In the other hand, the measuring method through the density has a good resolution, but the result using the initial density of the solution was different more than 10% error from that using the real-time density. And it has an error range 1.5 times greater than the method through the refractive index.
Wounds : a compendium of clinical research and practice, 2013
Silver plays an important part in severe wound management, mainly by reducing microbial growth wi... more Silver plays an important part in severe wound management, mainly by reducing microbial growth within dressed wounds and wound beds. However, it is unknown how silver-coated dressing materials affect diabetic wounds. The purpose of this study is to evaluate the efficacy of silver-containing dressing materials for the treatment of methicillin-resistant Staphylococcus aureus (MRSA)-infected wounds in streptozotocininduced diabetic rats. Full-thickness skin defects were created on the backs of rats with streptozotocin (STZ)-induced diabetes (n = 108) and were infected with MRSA. The rats were assigned to 6 groups according to the wound dressing used: nanocrystalline silver (Acticoat, Smith & Nephew, Inc, London, UK), silver carboxymethylcellulose (Aquacel-Ag, ConvaTec, Skillman, NJ), silver sulfadiazine (Medifoam silver, Genewel Science Co Ltd, Seongnam, South Korea), nanocrystalline silver (Poly- Mem silver, Ferris Mfg Corp, Burr Ridge, IL), silver sulfadiazine (Ilvadon, Ildong Pharma...
In this study, the use of canine experimental autoimmune encephalomyelitis (EAE) as a disease mod... more In this study, the use of canine experimental autoimmune encephalomyelitis (EAE) as a disease model for necrotizing encephalitis (NE) was assessed. Twelve healthy dogs (1-4 years old; 4.5-13.1 kg; 3 females/9 males; 9 Beagles/1 Schnauzer/2 Mongrels) were used in this study as experimental models. Canine forebrain tissue (8 g), including white and grey matter, were homogenized with 4 mL of phosphate-buffered saline for 5 min in an ice bath. The suspension was emulsified with the same volume of Freund's Complete Adjuvant containing 1 mg/mL of killed Mycobacterium tuberculosis H37Ra. Under sedation, each dog was injected subcutaneously with canine brain homogenate over 4 sites, with 2 in the inguinal and 2 in the axillary regions. A second injection (booster shot) was performed with the same procedure 7 days after the first injection in all dogs. Clinical assessments, magnetic resonance imaging, cerebrospinal fluid analyses, necropsies, and histopathological and immunohistochemical...
Cancer research and treatment : official journal of Korean Cancer Association, Jan 17, 2015
A 50-year-old woman was admitted to our hospital due to multiple lung nodules detected incidental... more A 50-year-old woman was admitted to our hospital due to multiple lung nodules detected incidentally on a chest X-ray. A video-assisted thoracoscopic lung biopsy revealed low-grade endometrial stromal sarcoma (LG-ESS). She had undergone a simple hysterectomy one year earlier owing to a diagnosis of adenomyosis. A review of her previous hysterectomy specimen showed not endometriosis but LG-ESS. According to the patient's levels of serum FSH and estradiol, she was in the pre-menopausal state with retained and normally functioning ovaries. She then underwent ovarian ablation by radiotherapy, after which she was administered 2.5 mg of letrozole once per day. Three months later, the size of the metastatic nodules in both lungs had decreased. The patient was followed up for 24 months while continuing on letrozole, and maintained a partial remission. We report herein on a case of metastatic LG-ESS treated with letrozole after ovarian ablation by radiotherapy.
An 8-year-old intact male Belgian Malinois, weighing 37.2 kg, was referred for evaluation due to ... more An 8-year-old intact male Belgian Malinois, weighing 37.2 kg, was referred for evaluation due to right side facial paresis, ataxia and a 2-month history of decreased cognitive ability. Physical and neurological examinations revealed mild depression, left-sided head tilt, right-sided facial paresis and ataxia. A well-demarcated, broad-based cerebellar mass and hyperostosis were found on CT imaging of the brain. Based on these CT findings, a cerebellar meningioma was strongly suspected. Hydroxyurea and prednisolone were administered; after 4 weeks, there was reduction in mass size as compared to initial CT results. However, the mass size was found to have grown 6 weeks after hydroxyurea treatment. We then prescribed a combination of imatinib mesylate and hydroxyurea. Two weeks following combination treatment, the mass size had reduced significantly. The mass continuously decreased in size until the patient died during anesthesia. Cerebellar transitional meningioma was confirmed by histopathologic examination. To the author's knowledge, this is the first reported case of imatinib mesylate plus hydroxyurea therapy for the treatment of meningioma in veterinary medicine.
ABSTRACT The purpose of this study is to merge clinical information from various neurological pat... more ABSTRACT The purpose of this study is to merge clinical information from various neurological patients of the Gyeongsang National University Animal Medical Center during the study period (from Jan.2008 to Jan.2012). Total 172 cases of neurological disorders were diagnosed and treated in the study period. Evaluated criteria of this study were breed, age, sex, clinical signs, and diagnosis. Physical examination, neurological examination, complete blood count, serum chemistry profile, radiography, ultrasonography, cerebrospinal fluid (CSF) analysis, computed tomography (CT), magnetic resonance imaging (MRI), and electroencephalography (EEG) were performed on the basis of individual cases. Among all of 172 dogs, 78 dogs (45.3%) were diagnosed with meningoencephalitis of unknown etiology (MUE); 38 dogs (22.0%) were intervertebral disk disease (IVDD); 15 dogs (8.7%) were idiopathic epilepsy; 13 dogs (7.5%) were hydrocephalus; 5 dogs (2.9%) were brain tumor; and the remainder was diagnosed with miscellaneous diseases. The canine neurological disease model is very useful to understand human neurological disease because the dog is structurally and functionally similar to the human and have same natural neurological diseases as human based on this study. Thus, we hope that this study could help to develop the canine neurological disease model.
A 4-year-old female Maltese (case 1), a 9-year-old castrated male shih tzu (case 2) and 2-year-ol... more A 4-year-old female Maltese (case 1), a 9-year-old castrated male shih tzu (case 2) and 2-year-old female Pomeranian (case 3) presented with neurological signs, such as head tilt, ataxia, circling and paresis. The three cases were tentatively diagnosed as having meningoencephalitis of unknown etiology based on computed tomography scan and cerebrospinal fluid analysis. All patients were managed with cyclosporine plus prednisolone therapy. The survival times of the three patients were 170, 70 and 21 days, respectively. After the cases died, we performed necropsy and histopathological examination for definitive diagnosis. Based on the necropsy, histopathological and immunohistochemical examinations, cases 1, 2 and 3 were definitely diagnosed as having necrotizing meningoencephalitis, necrotizing leukoencephalitis and granulomatous meningoencephalitis, respectively. This case report demonstrated the clinical findings, brain CT characteristics and histopathological and immunohistochemical features of NME, NLE and GME in dogs and discussed the reason for the relatively short survival times under cyclosporine plus prednisolone therapy.
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