Echinocystis lobata (Michx.) Torr. et A.Gray is a spontaneous species in the Romanian flora, less... more Echinocystis lobata (Michx.) Torr. et A.Gray is a spontaneous species in the Romanian flora, lesser studied by scientific literature, but which has proved significant activities in traditional medicine. The present study is aimed to provide data on the polyphenolic compounds in the composition of the flowers of this species and to test their biological potential. Polyphenols were identified and quantified using an HPLC-MS method. Tested biological activities were the cytotoxic, anti-plasmodial and antioxidant ones. Methods used for testing the antioxidant activity were the DPPH, CUPRAC, FRAP, TEAC, EPR and SNPAC assays. Cytotoxic activity was tested on cancerous and healthy cell lines and anti-plasmodial activity was assesed on two strains of Plasmodium falciparum. Ethanolic extracts of the flowers of E. lobata proved to contain isoquercitrin, rutin, quercitrin, kaempferol, p-coumaric and ferulic acid. No cytotoxic and anti-plasmodial activity was found, but antioxidant assays showed an important antioxidant capacity. The obtained results show that flowers of E. lobata are important sources of antioxidant compounds. It is the first approach of the kind on the flowers of this species and it offers a new perspective on possible sources of antioxidant compounds.
Bisphenol A (BPA), a food contaminant yielded by plastic packaging, is known as xenoestrogen and ... more Bisphenol A (BPA), a food contaminant yielded by plastic packaging, is known as xenoestrogen and oxidative stress inductor. Trifolium pratense L. (TP) is a natural source of phytoestrogens as well as of polyphenols. The aim of the present study was to evaluate the antioxidant potential and the protective effect of a TP hydroalcoholic extract in female rats subchronically exposed to BPA. Plasma samples were analysed after 30, 60 and 90 days of exposure to BPA (50 mg/kg bw) and/or a TP diluted tincture (2 mg total isoflavones / kg bw). Malondialdehyde (MDA), a lipid peroxidation end-product, and 2,3dihydroxybenzoic acid (2,3-DHBA), a marker of in vivo hydroxyl radical production, were used to evaluate the oxidative stress generation. BPA causes the increasing of total MDA plasma levels. The simultaneous administration of TP extract countered the effect of BPA on lipid peroxidation. BPA is not a significant source of free hydroxyl radicals in vivo. However, TP extract diminished in vivo hydroxyl radical generation compared with control group (p < 0.05 after 90 days). In conclusion, TP showed a protective antioxidant effect that increased with prolonged period of repeated administration. Rezumat Bisfenolul A (BPA), un contaminant alimentar cedat de ambalajele din plastic, are proprietăţi xenoestrogene si induce stres oxidativ. Trifolium pratense L. (TP) este o sursă naturală de fitoestrogeni si polifenoli. S-a evaluat potenţialul antioxidant si efectul protector al unui extract hidroalcoolic de TP la femele de sobolan expuse subcronic la BPA. S-au analizat probe de plasmă după 30, 60 si 90 de zile de expunere la BPA (50 mg/kg corp) si/sau tinctură diluată de TP (2 mg izoflavone totale / kg corp). Pentru evaluarea inducerii stresului oxidativ s-au determinat malondialdehida (MDA), un produs final al peroxidării lipidice, si acidul 2,3-dihidroxibenzoic (2,3-DHBA), un marker al producerii
Summary Introduction: Natural products represent a gold mine for scientists looking for compounds... more Summary Introduction: Natural products represent a gold mine for scientists looking for compounds for the treatment of health problems and diseases with their different biological and pharmacological activities. However, recent research is focused on finding natural sources of antioxidants. Objective: The objective of current research was to determine the phytochemical profile of Algerian fenu-greek (Trigonella foenum-graecum L.), and Syrian cumin (Cuminum cyminum L.) seeds in order to characterize their phenolic compounds and to determine their antioxidant activities. Methods: Total phenolic, flavonoids, condensed and hydrolysable tannins contents were quantified using Folin-Ciocalteu, aluminium chloride, vanillin and ferric chloride methods, respectively. Phenolic compounds were identified by HPLC method and antioxidant activity was measured using DPPH assay. Results: The higher amounts of total phenolic compounds, flavonoids, condensed and hydrolysable tannins were given by fenug...
Bisphenol A (BPA), a food contaminant yielded by plastic packaging, is known as xenoestrogen and ... more Bisphenol A (BPA), a food contaminant yielded by plastic packaging, is known as xenoestrogen and oxidative stress inductor. Trifolium pratense L. (TP) is a natural source of phytoestrogens as well as of polyphenols. The aim of the present study was to evaluate the antioxidant potential and the protective effect of a TP hydroalcoholic extract in female rats subchronically exposed to BPA. Plasma samples were analysed after 30, 60 and 90 days of exposure to BPA (50 mg/kg bw) and/or a TP diluted tincture (2 mg total isoflavones / kg bw). Malondialdehyde (MDA), a lipid peroxidation end-product, and 2,3dihydroxybenzoic acid (2,3-DHBA), a marker of in vivo hydroxyl radical production, were used to evaluate the oxidative stress generation. BPA causes the increasing of total MDA plasma levels. The simultaneous administration of TP extract countered the effect of BPA on lipid peroxidation. BPA is not a significant source of free hydroxyl radicals in vivo. However, TP extract diminished in viv...
Echinocystis lobata (Michx.) Torr. et A.Gray is a spontaneous species in the Romanian flora, less... more Echinocystis lobata (Michx.) Torr. et A.Gray is a spontaneous species in the Romanian flora, lesser studied by scientific literature, but which has proved significant activities in traditional medicine. The present study is aimed to provide data on the polyphenolic compounds in the composition of the flowers of this species and to test their biological potential. Polyphenols were identified and quantified using an HPLC-MS method. Tested biological activities were the cytotoxic, anti-plasmodial and antioxidant ones. Methods used for testing the antioxidant activity were the DPPH, CUPRAC, FRAP, TEAC, EPR and SNPAC assays. Cytotoxic activity was tested on cancerous and healthy cell lines and anti-plasmodial activity was assesed on two strains of Plasmodium falciparum. Ethanolic extracts of the flowers of E. lobata proved to contain isoquercitrin, rutin, quercitrin, kaempferol, p-coumaric and ferulic acid. No cytotoxic and anti-plasmodial activity was found, but antioxidant assays showed an important antioxidant capacity. The obtained results show that flowers of E. lobata are important sources of antioxidant compounds. It is the first approach of the kind on the flowers of this species and it offers a new perspective on possible sources of antioxidant compounds.
Bisphenol A (BPA), a food contaminant yielded by plastic packaging, is known as xenoestrogen and ... more Bisphenol A (BPA), a food contaminant yielded by plastic packaging, is known as xenoestrogen and oxidative stress inductor. Trifolium pratense L. (TP) is a natural source of phytoestrogens as well as of polyphenols. The aim of the present study was to evaluate the antioxidant potential and the protective effect of a TP hydroalcoholic extract in female rats subchronically exposed to BPA. Plasma samples were analysed after 30, 60 and 90 days of exposure to BPA (50 mg/kg bw) and/or a TP diluted tincture (2 mg total isoflavones / kg bw). Malondialdehyde (MDA), a lipid peroxidation end-product, and 2,3dihydroxybenzoic acid (2,3-DHBA), a marker of in vivo hydroxyl radical production, were used to evaluate the oxidative stress generation. BPA causes the increasing of total MDA plasma levels. The simultaneous administration of TP extract countered the effect of BPA on lipid peroxidation. BPA is not a significant source of free hydroxyl radicals in vivo. However, TP extract diminished in vivo hydroxyl radical generation compared with control group (p < 0.05 after 90 days). In conclusion, TP showed a protective antioxidant effect that increased with prolonged period of repeated administration. Rezumat Bisfenolul A (BPA), un contaminant alimentar cedat de ambalajele din plastic, are proprietăţi xenoestrogene si induce stres oxidativ. Trifolium pratense L. (TP) este o sursă naturală de fitoestrogeni si polifenoli. S-a evaluat potenţialul antioxidant si efectul protector al unui extract hidroalcoolic de TP la femele de sobolan expuse subcronic la BPA. S-au analizat probe de plasmă după 30, 60 si 90 de zile de expunere la BPA (50 mg/kg corp) si/sau tinctură diluată de TP (2 mg izoflavone totale / kg corp). Pentru evaluarea inducerii stresului oxidativ s-au determinat malondialdehida (MDA), un produs final al peroxidării lipidice, si acidul 2,3-dihidroxibenzoic (2,3-DHBA), un marker al producerii
Summary Introduction: Natural products represent a gold mine for scientists looking for compounds... more Summary Introduction: Natural products represent a gold mine for scientists looking for compounds for the treatment of health problems and diseases with their different biological and pharmacological activities. However, recent research is focused on finding natural sources of antioxidants. Objective: The objective of current research was to determine the phytochemical profile of Algerian fenu-greek (Trigonella foenum-graecum L.), and Syrian cumin (Cuminum cyminum L.) seeds in order to characterize their phenolic compounds and to determine their antioxidant activities. Methods: Total phenolic, flavonoids, condensed and hydrolysable tannins contents were quantified using Folin-Ciocalteu, aluminium chloride, vanillin and ferric chloride methods, respectively. Phenolic compounds were identified by HPLC method and antioxidant activity was measured using DPPH assay. Results: The higher amounts of total phenolic compounds, flavonoids, condensed and hydrolysable tannins were given by fenug...
Bisphenol A (BPA), a food contaminant yielded by plastic packaging, is known as xenoestrogen and ... more Bisphenol A (BPA), a food contaminant yielded by plastic packaging, is known as xenoestrogen and oxidative stress inductor. Trifolium pratense L. (TP) is a natural source of phytoestrogens as well as of polyphenols. The aim of the present study was to evaluate the antioxidant potential and the protective effect of a TP hydroalcoholic extract in female rats subchronically exposed to BPA. Plasma samples were analysed after 30, 60 and 90 days of exposure to BPA (50 mg/kg bw) and/or a TP diluted tincture (2 mg total isoflavones / kg bw). Malondialdehyde (MDA), a lipid peroxidation end-product, and 2,3dihydroxybenzoic acid (2,3-DHBA), a marker of in vivo hydroxyl radical production, were used to evaluate the oxidative stress generation. BPA causes the increasing of total MDA plasma levels. The simultaneous administration of TP extract countered the effect of BPA on lipid peroxidation. BPA is not a significant source of free hydroxyl radicals in vivo. However, TP extract diminished in viv...
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Papers by Daniela Hanganu