On 14th October 2021, a single specimen of Sargocentron caudimaculatum was captured in the locali... more On 14th October 2021, a single specimen of Sargocentron caudimaculatum was captured in the locality of Cape Bon, Eastern Tunisia, by a local fisher using gillnets at 60 m depth, on rocky bottom. This observation represents the first record of this species in the Mediterranean Sea. General information on the squirrelfishes and the importance of molecular tools for the proper identification of morphologically challenging non-indigenous organisms were raised and discussed.
Plasmid-mediated beta-lactamases (extended spectrum beta-lactamases (ESBLs) and AmpC β-lactamases... more Plasmid-mediated beta-lactamases (extended spectrum beta-lactamases (ESBLs) and AmpC β-lactamases (pAmpC)) have been of great concern worldwide. The rapid increase in the proportion of ESBL-producing E. coli in alimentary tract of food producing and pet animals have been occurred globally since 2000s [1]. Therefore, prevalence of ESBLproducing E. coli has been also appeared to be in increase in various foodstuffs including raw milk, dairy products and meat, particularly chicken meat [2]. Retail chicken meat contaminated by E. coli producing ESBLs or pAmpC has been increasingly reported worldwide [3,4]. Many reports have described the close genetic relatedness of E. coli isolates of animal and human origins. Such transmission (animal to human) is likely occurred by physic contacts or by consummation of food products containing resistant E. coli isolates. This phenomenon likely contributes to the increased incidence of infections with these bacteria in humans [2,5]. The aims of this s...
Little is known about the role of forestland and non-fertilized agriculture soils as reservoirs o... more Little is known about the role of forestland and non-fertilized agriculture soils as reservoirs of extended-spectrum beta-lactamase (ESBL) and plasmid-borne AmpC (pAmpC)-producing Escherichia coli isolates. Thus, in the present study, 210 soil samples from various origins (forest of Oued Zen (Ain Drahem), non-agriculture soils from different park gardens in Tunis City, cereal culture soils and home gardens) were investigated to characterize cefotaxime-resistant E. coli isolates. A total of 22 ESBL/pAmpC-producing E. coli were collected, and all harbored variants of the blaCTX-M gene (15 blaCTX-M-1, 5 blaCTX-M-55 and 2 blaCTX-M-15). A total of seven and two isolates harbored also blaEBC and blaDHA-like genes, respectively. Resistances to tetracycline, sulfonamides and fluoroquinolones were encoded by tetA (n = 4)/tetB (n = 12), sul1 (n = 17)/sul2 (n = 19) and aac(6’)-Ib-cr (n = 2)/qnrA (n = 1)/qnrS (n = 1) genes, respectively. A total of seven isolates were able to transfer by conjug...
The aim of this work was the genetic characterization of cefotaxime-resistant enterobacteria from... more The aim of this work was the genetic characterization of cefotaxime-resistant enterobacteria from animals (53 samples), the surface water of rivers (17 samples), and wastewater treatment plants (43 samples) in Tunisia. A total of 48 (42.4%) cefotaxime-resistant isolates were recovered. An extended spectrum beta-lactamase (ESBL) phenotype with a positive double-disk synergy test (DDST) was exhibited by 34 (70.8%) and 14 (29.1%) isolates from water and animal origins, respectively. Isolates from water were identified as: Escherichia coli (n = 17), Hafnia spp. (n = 13), Citrobacter spp. (n = 1), Enterobacter cloacae (n = 1), Klebsiella pneumoniae (n = 1), and K. oxytoca (n = 1). Animal isolates were identified as: E. coli (n = 11), E. cloacae (n = 1), Hafnia spp. (n = 1), and K. pneumoniae (n = 1). PCR investigation of blaCTX-M, blaTEM, and blaSHV genes showed that amongst the 48 isolates with a positive DDST, 41 (87.5%) carried the blaCTX-M gene, 1 isolate harbored the blaSHV gene, an...
The valorization of different organic residues like municipal solid wastes, sewage sludge and oli... more The valorization of different organic residues like municipal solid wastes, sewage sludge and olive mill wastewater is becoming more and more worrying in the different modern communities and is becoming relevant and crucial in terms of environmental preservation. The choice of the treatment technique should not be only from the point of view of economic profitability but, above all, must consider the efficiency of the treatment method. Thus, an attempt to remove polyphenols from olive mill wastewater would have a double interest: on the one hand, to solve a major environmental problem and to recover and valorize the olive mill wastewater for advanced applications in food processing and soil amendments. It is also interesting to think of associating two harmful wastes by co-composting such as sewage sludge-vegetable gardens, sewage sludge-municipal solid waste, and green wastes-olive mill wastewater…, to get a mixed compost of good physical–chemical and biological qualities useful fo...
On 14th October 2021, a single specimen of Sargocentron caudimaculatum was captured in the locali... more On 14th October 2021, a single specimen of Sargocentron caudimaculatum was captured in the locality of Cape Bon, Eastern Tunisia, by a local fisher using gillnets at 60 m depth, on rocky bottom. This observation represents the first record of this species in the Mediterranean Sea. General information on the squirrelfishes and the importance of molecular tools for the proper identification of morphologically challenging non-indigenous organisms were raised and discussed.
Plasmid-mediated beta-lactamases (extended spectrum beta-lactamases (ESBLs) and AmpC β-lactamases... more Plasmid-mediated beta-lactamases (extended spectrum beta-lactamases (ESBLs) and AmpC β-lactamases (pAmpC)) have been of great concern worldwide. The rapid increase in the proportion of ESBL-producing E. coli in alimentary tract of food producing and pet animals have been occurred globally since 2000s [1]. Therefore, prevalence of ESBLproducing E. coli has been also appeared to be in increase in various foodstuffs including raw milk, dairy products and meat, particularly chicken meat [2]. Retail chicken meat contaminated by E. coli producing ESBLs or pAmpC has been increasingly reported worldwide [3,4]. Many reports have described the close genetic relatedness of E. coli isolates of animal and human origins. Such transmission (animal to human) is likely occurred by physic contacts or by consummation of food products containing resistant E. coli isolates. This phenomenon likely contributes to the increased incidence of infections with these bacteria in humans [2,5]. The aims of this s...
Little is known about the role of forestland and non-fertilized agriculture soils as reservoirs o... more Little is known about the role of forestland and non-fertilized agriculture soils as reservoirs of extended-spectrum beta-lactamase (ESBL) and plasmid-borne AmpC (pAmpC)-producing Escherichia coli isolates. Thus, in the present study, 210 soil samples from various origins (forest of Oued Zen (Ain Drahem), non-agriculture soils from different park gardens in Tunis City, cereal culture soils and home gardens) were investigated to characterize cefotaxime-resistant E. coli isolates. A total of 22 ESBL/pAmpC-producing E. coli were collected, and all harbored variants of the blaCTX-M gene (15 blaCTX-M-1, 5 blaCTX-M-55 and 2 blaCTX-M-15). A total of seven and two isolates harbored also blaEBC and blaDHA-like genes, respectively. Resistances to tetracycline, sulfonamides and fluoroquinolones were encoded by tetA (n = 4)/tetB (n = 12), sul1 (n = 17)/sul2 (n = 19) and aac(6’)-Ib-cr (n = 2)/qnrA (n = 1)/qnrS (n = 1) genes, respectively. A total of seven isolates were able to transfer by conjug...
The aim of this work was the genetic characterization of cefotaxime-resistant enterobacteria from... more The aim of this work was the genetic characterization of cefotaxime-resistant enterobacteria from animals (53 samples), the surface water of rivers (17 samples), and wastewater treatment plants (43 samples) in Tunisia. A total of 48 (42.4%) cefotaxime-resistant isolates were recovered. An extended spectrum beta-lactamase (ESBL) phenotype with a positive double-disk synergy test (DDST) was exhibited by 34 (70.8%) and 14 (29.1%) isolates from water and animal origins, respectively. Isolates from water were identified as: Escherichia coli (n = 17), Hafnia spp. (n = 13), Citrobacter spp. (n = 1), Enterobacter cloacae (n = 1), Klebsiella pneumoniae (n = 1), and K. oxytoca (n = 1). Animal isolates were identified as: E. coli (n = 11), E. cloacae (n = 1), Hafnia spp. (n = 1), and K. pneumoniae (n = 1). PCR investigation of blaCTX-M, blaTEM, and blaSHV genes showed that amongst the 48 isolates with a positive DDST, 41 (87.5%) carried the blaCTX-M gene, 1 isolate harbored the blaSHV gene, an...
The valorization of different organic residues like municipal solid wastes, sewage sludge and oli... more The valorization of different organic residues like municipal solid wastes, sewage sludge and olive mill wastewater is becoming more and more worrying in the different modern communities and is becoming relevant and crucial in terms of environmental preservation. The choice of the treatment technique should not be only from the point of view of economic profitability but, above all, must consider the efficiency of the treatment method. Thus, an attempt to remove polyphenols from olive mill wastewater would have a double interest: on the one hand, to solve a major environmental problem and to recover and valorize the olive mill wastewater for advanced applications in food processing and soil amendments. It is also interesting to think of associating two harmful wastes by co-composting such as sewage sludge-vegetable gardens, sewage sludge-municipal solid waste, and green wastes-olive mill wastewater…, to get a mixed compost of good physical–chemical and biological qualities useful fo...
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