A simple, new, green, and sensitive approach was established and validated for assay of the recen... more A simple, new, green, and sensitive approach was established and validated for assay of the recently approved antineoplastic medication; darolutamide (DAR) in its authentic form, pharmaceutical formulation, and biological fluids fluorimetrically.
Oral, quick response, and on demand, also known as a spontaneous oral treatment for erectile dysf... more Oral, quick response, and on demand, also known as a spontaneous oral treatment for erectile dysfunction, is highly needed by both patients and physicians. Vardenafil is selective (fewer side effects) and more effective in difficult-to-treat conditions than sildenafil. This study aims at fostering the dual objectives of using biomolecules such as artificial sweetening agents to solubilize and mask the bitterness of vardenafil loaded on biodegradable polymeric materials (PVA, MC, SA, and PVP K30) to fabricate oral, fast-dissolving films (vardenafil ODFs) in the mouth without the need for water to ingest the dosage form. Furthermore, coprecipitated-dispersed mixtures of vardenafil and three sweeteners (sorbitol, acesulfame K, and sucralose) were prepared and characterized using FTIR, DSC, and solubility studies. Moreover, eight different vardenafil ODFs were prepared using the solvent-casting method. Modified gustatory sensation test, in vitro disintegration, and release studies were ...
The construction and electrochemical response characteristics of six Ciprofloxacin-selective elec... more The construction and electrochemical response characteristics of six Ciprofloxacin-selective electrodes were investigated using precipitation based technique with sodium tetraphenyl borate (TPB), phosphomolybdate (PMA) and phosphotungstate (PTA); respectively upon using polyvinyl chloride (PVC) matrix and dibutyl phthalate (DBP) as a plasticizer. The resultant sensors have different forms, either as membrane electrodes (sensors 1, 3 and 5) or as coated wire electrodes (sensors 2, 4 and 6). Linear responses of CIP within the concentration ranges of 10 to 10 mol/L for sensors 1, 2 and 5 while for sensors 3 and 4, the linear responses were within the concentration ranges of 10 −5 to 10 −2 mol/L and for sensor 6 it shows linear responses within the concentration ranges of 10 to 10 mol/L. Nernstian slopes of 51.7, 50.7, 58.3, 57.7, 44 and 41.8 mV/decade were observed over the pH range of (5–9) for sensors 1, 2, 5 and 6 and over range of (5-7) for sensor 3 and 4 respectively. The selectiv...
Simple and accurate methods are described for the quantitative determination of flufenamic acid, ... more Simple and accurate methods are described for the quantitative determination of flufenamic acid, mefenamic acid and tranexamic acid utilizing precipitation reactions with cobalt, cadmium and manganese. The acidic drugs were precipitated from their neutral alcoholic solutions with cobalt sulphate, cadmium nitrate or manganese chloride standard solutions followed by direct determination of the ions in the precipitate or indirect determination of the ions in the filtrate by atomic absorption spectroscopy. The optimum conditions for precipitation were carefully studied. The molar ratio of the reactants was ascertained. Statistical analysis of the results compared to the results of the official methods revealed equal precision and accuracy. The suggested procedures were applied for determining flufenamic acid, mefenamic acid and tranexamic acid in pharmaceutical preperations as well and proved validity.
A simple and accurate method is described for the quantitative determination of flufenamic acid, ... more A simple and accurate method is described for the quantitative determination of flufenamic acid, mefenamic acid, tranexamic acid, furosemide, diclofenac sodium and thiaprofenic acid by precipitation reactions with nickel(II) followed by direct determination of the ions in the precipitate or indirect determination of the ions in the filtrate by atomic absorption spectroscopy. Statistical analysis of the results compared to assays used in pharmacopeas and the Amax. methods revealed equal precision and accuracy. Furthermore the assays were also applied for the determination of these drugs in pharmaceutical preparations.
A new simple, precise, accurate and selective high-performance thin-layer chromatographic (HPTLC)... more A new simple, precise, accurate and selective high-performance thin-layer chromatographic (HPTLC) method has been developed for the simultaneous deter-mination of five mixtures: atenolol and chlorthalidone (Mix. l), enalapril maleate and hydrochlorothiazide (Mix. ll), amiloride hydrochloride and hydrochlorothiazide (Mix. lll), atenolol, chlorthalidone and amiloride hydrochloride (Mix. lV) and atenolol, hydrochlorottriazide and amiloride hydrochloride (Mix. V) in bulk powders and in pharmaceutical dosage forms. The methods consist of dissolving the drugs in methanol and spotting these solutions on a thin layer Merck HPTLC plates (0.25 mm thickness) pre-coated wrth 60 GF254 silica gel on aluminum sheet as the stationary phase, using dioxane:acetonrtrile: 1-propanol:hexane (30:18:23:1; v/v/v/v) , ethylacetate:chlo-roform:methanol:acetic acid (11:8:7.5:1.5; v/v/v/v). ethylacetate:chloroform:1-propanol:25% ammonia solution (12:9:1:0.2; v/v/v/v), di0xan:acetonitrile:1-propanol:tetrahydrof...
Objectives To investigate the possible modification of the cardioprotective effect of amlodipine ... more Objectives To investigate the possible modification of the cardioprotective effect of amlodipine when co-administered with quercetin in myocardial ischaemia/reperfusion-induced functional, metabolic and cellular alterations in rats. Methods Oral doses of amlodipine (15 mg/kg) and quercetin (5 mg/kg), alone or in combination, were administered once daily for 1 week. Rats were then subjected to myocardial ischaemia/reperfusion (35min/10min). Heart rates and ventricular arrhythmias were recorded during ischaemia/reperfusion progress. At the end of reperfusion, activities of plasma creatine kinase (CK) and cardiac myeloperoxidase were determined. In addition, cardiac contents of lactate, ATP, thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH) and total nitrate/nitrite (NOx) were estimated. Finally, histological examination was performed to visualize the protective cellular effects of different pretreatments. Key findings Combined therapy provided significant impr...
Four simple and selective spectrophotometric methods were developed for the quantitative determin... more Four simple and selective spectrophotometric methods were developed for the quantitative determination of some phenolic β-lactam antibiotics (amoxicillin trihydrate, cefoperazone sodium, cefadroxil monohydrate, and cefprozil anhydrous) in pure forms as well as in their pharmaceutical formulations through their nitration and subsequent complexation with an nucleophilic reagent (method I), nitrosation and subsequent metal chelation (method II), coupling with diazo reagent (method
An inexpensive, simple, sensitive and validated approach is developed for estimation of fingolimo... more An inexpensive, simple, sensitive and validated approach is developed for estimation of fingolimod through production of colored charge transfer complexes of fingolimod with different electron acceptor reagents, including a reaction of fingolimod as n-donor with 7,7,8,8-tetracyanoquinodimethane, tetrachloro 1,4-benzoquinone and tetracyanoethylene and as n-acceptors, yielding colored and stable anions which were measured spectrophotometrically. The range that obeyed Beer’s law is 50–300 µg mL−1 for fingolimod with all the studied reagents. The various parameters that affect the reaction were studied and optimized. The results were statistically compared with a reported method showing equal precision and accuracy. The researched approaches were utilized to determine the cited drug in its pharmaceutical form and spiked human plasma with accepted accuracy and precision.
A direct colorimetric method was described for the rapid, sensitive and accurate determination of... more A direct colorimetric method was described for the rapid, sensitive and accurate determination of dibucaine, lidocaine, bupivacaine, procaine and tetracaine in pharmaceutical preparations. The method involves the use of haematoxylin reagent in the presence of boric acid to give a reddish-violet chromogen (λmax = 555 nm). Beer's law was obeyed in the range from 2–60 μg/ml. No interference was observed
International Research Journal of Pure and Applied Chemistry, 2021
A dependable, sensitive, basic and cheap spectrofluorimetric approach has been created for test o... more A dependable, sensitive, basic and cheap spectrofluorimetric approach has been created for test of sulfur-containing drug; ixabepilone in bulk powder, vials and human plasma. The approach depends on the quenching effect of ixabepilone on the fluorescence intensity of acetoxymercuric fluorescene (AMF) reagent at λem of 530 nm and λex of 500 nm. Parameters which will control the reaction such as pH, AMF solution concentration, temperature, time and solvents were examined and optimized. According to the optimized conditions, the proposed approach was practiced over the concentration area of 20-100 ng mL-1 with adequate linearity (r = 0.9998). The developed approach was approved confirming to ICH rules in terms of accuracy, precision, linearity, LOD and LOQ. The proposed approach was practiced to analyze ixabepilone in Ixempra® vials with satisfactory recovery % of 99.89 and RSE% of 1.24. The results achieved were compared to those achieved by an already reported HPLC approach.
A spectrofluorimetric approach has been developed and validated for determination of sulfur-conta... more A spectrofluorimetric approach has been developed and validated for determination of sulfur-containing drug; ixabepilone in raw powder, vials and human plasma. This approach studies the quenching effect of IXA on the fluorescence intensity of acetoxymercuric fluorescein (AMF) reagent at λem of 530 nm and λex of 500 nm. All the parameters that can affect the reaction as pH, AMF solution concentration, temperature, time and solvents were studied and optimized. The linearity range of the studied approach was 20-100 ng mL-1 with correlation coefficient of (r = 0.9998). The proposed approach was validated and approved regarding to ICH guidelines in terms of accuracy, precision, linearity, LOD and LOQ, with mean percentage recovery of 99.79 and RSE% of 1.64. The previously obtained resultes were already statistically compared with that of established reported methods indicating no significant differences in accuracy and precision. Finally, the proposed approach is easy, sensitive, and ine...
A simple, new, green, and sensitive approach was established and validated for assay of the recen... more A simple, new, green, and sensitive approach was established and validated for assay of the recently approved antineoplastic medication; darolutamide (DAR) in its authentic form, pharmaceutical formulation, and biological fluids fluorimetrically.
Oral, quick response, and on demand, also known as a spontaneous oral treatment for erectile dysf... more Oral, quick response, and on demand, also known as a spontaneous oral treatment for erectile dysfunction, is highly needed by both patients and physicians. Vardenafil is selective (fewer side effects) and more effective in difficult-to-treat conditions than sildenafil. This study aims at fostering the dual objectives of using biomolecules such as artificial sweetening agents to solubilize and mask the bitterness of vardenafil loaded on biodegradable polymeric materials (PVA, MC, SA, and PVP K30) to fabricate oral, fast-dissolving films (vardenafil ODFs) in the mouth without the need for water to ingest the dosage form. Furthermore, coprecipitated-dispersed mixtures of vardenafil and three sweeteners (sorbitol, acesulfame K, and sucralose) were prepared and characterized using FTIR, DSC, and solubility studies. Moreover, eight different vardenafil ODFs were prepared using the solvent-casting method. Modified gustatory sensation test, in vitro disintegration, and release studies were ...
The construction and electrochemical response characteristics of six Ciprofloxacin-selective elec... more The construction and electrochemical response characteristics of six Ciprofloxacin-selective electrodes were investigated using precipitation based technique with sodium tetraphenyl borate (TPB), phosphomolybdate (PMA) and phosphotungstate (PTA); respectively upon using polyvinyl chloride (PVC) matrix and dibutyl phthalate (DBP) as a plasticizer. The resultant sensors have different forms, either as membrane electrodes (sensors 1, 3 and 5) or as coated wire electrodes (sensors 2, 4 and 6). Linear responses of CIP within the concentration ranges of 10 to 10 mol/L for sensors 1, 2 and 5 while for sensors 3 and 4, the linear responses were within the concentration ranges of 10 −5 to 10 −2 mol/L and for sensor 6 it shows linear responses within the concentration ranges of 10 to 10 mol/L. Nernstian slopes of 51.7, 50.7, 58.3, 57.7, 44 and 41.8 mV/decade were observed over the pH range of (5–9) for sensors 1, 2, 5 and 6 and over range of (5-7) for sensor 3 and 4 respectively. The selectiv...
Simple and accurate methods are described for the quantitative determination of flufenamic acid, ... more Simple and accurate methods are described for the quantitative determination of flufenamic acid, mefenamic acid and tranexamic acid utilizing precipitation reactions with cobalt, cadmium and manganese. The acidic drugs were precipitated from their neutral alcoholic solutions with cobalt sulphate, cadmium nitrate or manganese chloride standard solutions followed by direct determination of the ions in the precipitate or indirect determination of the ions in the filtrate by atomic absorption spectroscopy. The optimum conditions for precipitation were carefully studied. The molar ratio of the reactants was ascertained. Statistical analysis of the results compared to the results of the official methods revealed equal precision and accuracy. The suggested procedures were applied for determining flufenamic acid, mefenamic acid and tranexamic acid in pharmaceutical preperations as well and proved validity.
A simple and accurate method is described for the quantitative determination of flufenamic acid, ... more A simple and accurate method is described for the quantitative determination of flufenamic acid, mefenamic acid, tranexamic acid, furosemide, diclofenac sodium and thiaprofenic acid by precipitation reactions with nickel(II) followed by direct determination of the ions in the precipitate or indirect determination of the ions in the filtrate by atomic absorption spectroscopy. Statistical analysis of the results compared to assays used in pharmacopeas and the Amax. methods revealed equal precision and accuracy. Furthermore the assays were also applied for the determination of these drugs in pharmaceutical preparations.
A new simple, precise, accurate and selective high-performance thin-layer chromatographic (HPTLC)... more A new simple, precise, accurate and selective high-performance thin-layer chromatographic (HPTLC) method has been developed for the simultaneous deter-mination of five mixtures: atenolol and chlorthalidone (Mix. l), enalapril maleate and hydrochlorothiazide (Mix. ll), amiloride hydrochloride and hydrochlorothiazide (Mix. lll), atenolol, chlorthalidone and amiloride hydrochloride (Mix. lV) and atenolol, hydrochlorottriazide and amiloride hydrochloride (Mix. V) in bulk powders and in pharmaceutical dosage forms. The methods consist of dissolving the drugs in methanol and spotting these solutions on a thin layer Merck HPTLC plates (0.25 mm thickness) pre-coated wrth 60 GF254 silica gel on aluminum sheet as the stationary phase, using dioxane:acetonrtrile: 1-propanol:hexane (30:18:23:1; v/v/v/v) , ethylacetate:chlo-roform:methanol:acetic acid (11:8:7.5:1.5; v/v/v/v). ethylacetate:chloroform:1-propanol:25% ammonia solution (12:9:1:0.2; v/v/v/v), di0xan:acetonitrile:1-propanol:tetrahydrof...
Objectives To investigate the possible modification of the cardioprotective effect of amlodipine ... more Objectives To investigate the possible modification of the cardioprotective effect of amlodipine when co-administered with quercetin in myocardial ischaemia/reperfusion-induced functional, metabolic and cellular alterations in rats. Methods Oral doses of amlodipine (15 mg/kg) and quercetin (5 mg/kg), alone or in combination, were administered once daily for 1 week. Rats were then subjected to myocardial ischaemia/reperfusion (35min/10min). Heart rates and ventricular arrhythmias were recorded during ischaemia/reperfusion progress. At the end of reperfusion, activities of plasma creatine kinase (CK) and cardiac myeloperoxidase were determined. In addition, cardiac contents of lactate, ATP, thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH) and total nitrate/nitrite (NOx) were estimated. Finally, histological examination was performed to visualize the protective cellular effects of different pretreatments. Key findings Combined therapy provided significant impr...
Four simple and selective spectrophotometric methods were developed for the quantitative determin... more Four simple and selective spectrophotometric methods were developed for the quantitative determination of some phenolic β-lactam antibiotics (amoxicillin trihydrate, cefoperazone sodium, cefadroxil monohydrate, and cefprozil anhydrous) in pure forms as well as in their pharmaceutical formulations through their nitration and subsequent complexation with an nucleophilic reagent (method I), nitrosation and subsequent metal chelation (method II), coupling with diazo reagent (method
An inexpensive, simple, sensitive and validated approach is developed for estimation of fingolimo... more An inexpensive, simple, sensitive and validated approach is developed for estimation of fingolimod through production of colored charge transfer complexes of fingolimod with different electron acceptor reagents, including a reaction of fingolimod as n-donor with 7,7,8,8-tetracyanoquinodimethane, tetrachloro 1,4-benzoquinone and tetracyanoethylene and as n-acceptors, yielding colored and stable anions which were measured spectrophotometrically. The range that obeyed Beer’s law is 50–300 µg mL−1 for fingolimod with all the studied reagents. The various parameters that affect the reaction were studied and optimized. The results were statistically compared with a reported method showing equal precision and accuracy. The researched approaches were utilized to determine the cited drug in its pharmaceutical form and spiked human plasma with accepted accuracy and precision.
A direct colorimetric method was described for the rapid, sensitive and accurate determination of... more A direct colorimetric method was described for the rapid, sensitive and accurate determination of dibucaine, lidocaine, bupivacaine, procaine and tetracaine in pharmaceutical preparations. The method involves the use of haematoxylin reagent in the presence of boric acid to give a reddish-violet chromogen (λmax = 555 nm). Beer's law was obeyed in the range from 2–60 μg/ml. No interference was observed
International Research Journal of Pure and Applied Chemistry, 2021
A dependable, sensitive, basic and cheap spectrofluorimetric approach has been created for test o... more A dependable, sensitive, basic and cheap spectrofluorimetric approach has been created for test of sulfur-containing drug; ixabepilone in bulk powder, vials and human plasma. The approach depends on the quenching effect of ixabepilone on the fluorescence intensity of acetoxymercuric fluorescene (AMF) reagent at λem of 530 nm and λex of 500 nm. Parameters which will control the reaction such as pH, AMF solution concentration, temperature, time and solvents were examined and optimized. According to the optimized conditions, the proposed approach was practiced over the concentration area of 20-100 ng mL-1 with adequate linearity (r = 0.9998). The developed approach was approved confirming to ICH rules in terms of accuracy, precision, linearity, LOD and LOQ. The proposed approach was practiced to analyze ixabepilone in Ixempra® vials with satisfactory recovery % of 99.89 and RSE% of 1.24. The results achieved were compared to those achieved by an already reported HPLC approach.
A spectrofluorimetric approach has been developed and validated for determination of sulfur-conta... more A spectrofluorimetric approach has been developed and validated for determination of sulfur-containing drug; ixabepilone in raw powder, vials and human plasma. This approach studies the quenching effect of IXA on the fluorescence intensity of acetoxymercuric fluorescein (AMF) reagent at λem of 530 nm and λex of 500 nm. All the parameters that can affect the reaction as pH, AMF solution concentration, temperature, time and solvents were studied and optimized. The linearity range of the studied approach was 20-100 ng mL-1 with correlation coefficient of (r = 0.9998). The proposed approach was validated and approved regarding to ICH guidelines in terms of accuracy, precision, linearity, LOD and LOQ, with mean percentage recovery of 99.79 and RSE% of 1.64. The previously obtained resultes were already statistically compared with that of established reported methods indicating no significant differences in accuracy and precision. Finally, the proposed approach is easy, sensitive, and ine...
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