The hexavalent chromium Cr(VI) poses a threat as a hazardous metal and its removal from aquatic e... more The hexavalent chromium Cr(VI) poses a threat as a hazardous metal and its removal from aquatic environments through biosorption has gained attention as a viable technology of bioremediation. We evaluated the potential use of three green algae (Cladophora glomerata, Enteromorpha intestinalis and Microspora amoena) dry biomass as a biosorbent to remove Cr(VI) from aqueous solutions. The adsorption capacity of the biomass was determined using batch experiments. The adsorption capacity appeared to depend on the pH. The optimum pH with the acid-treated biomass for Cr(VI) biosorption was found to be 2.0 at a constant temperature, 45°C. Among the three genera studied, C. glomerata recorded a maximum of 66.6% removal from the batch process using 1.0 g dried algal cells/100 ml aqueous solution containing an initial concentration of 20 mg/L chromium at 45°C and pH 2.0 for 60 min of contact time. Langmuir and Freundlich isotherm equations fitted to the equilibrium data, Freundlich was the better model. Our study showed that C. glomerata dry biomass is a suitable candidate to remove Cr(VI) from aqueous solutions.
Journal of Pharmaceutical Research International, 2021
Background: Hepatotoxicity was one of the major side effects associated with doxorubicin treatmen... more Background: Hepatotoxicity was one of the major side effects associated with doxorubicin treatment in cancer chemotherapy. The synthesized silver nanoparticles (AgNPs) from natural products such as algae especially green algae is one of the favorable means to minimize the deleterious effects of the chemotherapy. Thus, this study aimed to evaluate the preventive role of AgNPs synthesized by Ulva fasciata (U. fasciata) against doxorubicin-induced hepatotoxicity and oxidative stress in the liver of male Wistar rats. Materials and Methods: In the present study, the green macroalga U. fasciata ethanolic extract was used as reducing agents to reduce Ag ions to Ag0. Doxorubicin-injected male Wistar rats were concomitantly treated with U. fasciata ethanolic extract and AgNPs synthesized by U. fasciata extract (AgNPs/U. fasciata) 3 times/week by oral gavage for 6 weeks. Results: The results showed that male Wistar rats injected with doxorubicin showed a significant increase in ALT, ALP and ...
The aim of this study is to provide the seasonal variations in the coastal macroalgal communities... more The aim of this study is to provide the seasonal variations in the coastal macroalgal communities which inhabiting the upper intertidal zone of Hurghada, Safaga and Qusier coasts at the Red Sea, Egypt. The distribution of algal communities, degree of relative dominance and diversity of the species in different habitats were investigated from autumn 2005 up to summer 2006. Also, the changes in the environmental factors and the abundance of the algal species were correlated. A total of 35 algal species were recorded in the studied area, 7, 7 and 21 species of them belong to Chlorophyta, Rhodophyta and Phaeophyta, respectively. Qusier coast had the highest account of individuals / m (12.71) and total covering (60 %) compared with Hurghada and Safaga coasts. Both Chlorophyta and Rhodophyta were more abundant (66.67 and 48.15 %, respectively) and widely distributed during the summer season along Hurghada and Safaga coasts. Meanwhile, Phaeophyta highest percentage (74.05%) was during autu...
Microalgae sorbents are microalgae that have the potential to passively bind heavy metals/contami... more Microalgae sorbents are microalgae that have the potential to passively bind heavy metals/contaminants to their cellular structures in a process called biosorption. This study investigates the use of two species of microalgae to remove the toxic heavy metal cobalt from aqueous solution. Two microalgae isolates, Phormidium tenue and Chlorella vulgaris, were collected from the Wadi Hanifah Stream in Riyadh, the Kingdom of Saudi Arabia. We determined the capacity of both isolates to bioremove Co+2 ions and the optimum conditions under which this occurs. The two isolates were additionally characterized by microscopic and Fourier transform infrared spectroscopy (FTIR). In the current investigation, Phormidium tenue removed 94% of Co+2 under ideal conditions of pH 6, contact duration (30 min), starting concentration (50 mgL-1) and biosorbent dose (1gL-1); while Chlorella vulgaris removed 87% of Co+2 under the same parameters except pH 5.5 and contact duration (60 min). Fourier transform infrared spectroscopy (FTIR) confirms the binding of Co+2 to the biomass, which comprises many of the functional groups. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed some alterations to the shape of algal cells and cellular components for both microalgae studied. In addition, equilibrium study by both Langmuir and Freundlich models was performed to detect the effect of certain equilibrium factors on the capacity of the biosorption mechanism. Finally, Phormidium tenue and Chlorella vulgaris were discovered to be promising microalgae for effective cobalt biosorption in aquatic conditions.
The exploration of novel therapeutic agents and other bioactive secondary metabolite from Strepto... more The exploration of novel therapeutic agents and other bioactive secondary metabolite from Streptomyces species, for possible agricultural farming, pharmaceutical and industrialized applications, has been, and still is, essential. The existing studies were aimed with biologically potential Streptomyces species and its antagonistic activity against dreadful microorganisms. Totally, morphological three different actinomycetes were selected from the fertile agricultural lands. Among the three, the isolate SA4 exhibited significant antimicrobial and anti-nematicidal activity towards selected microbial pathogens such as E Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, Salmonella typhi, Bacillus subtilis, Proteus vulgaris, Shigella flexineri, Candida albicans, and Fusarium sp. The prospective strain SA4 was identified as Streptomyces cuspidosporus. The isolate SA4 optimized for secondary metabolites production with International Streptomyces project 4 (ISP 4) medium, pH 7.0 at 37°C for 14 days. Gas Chromatography-Mass spectrometry (GCMS) analysis of strain SA4 bioactive extract publicized the existence of 1,2-Benzenedicarboxylic acid, bis(2-Methylpropyl) ester compound and occupied by high peak area and its possessed significant biological properties.
Diethyl ether, acetone and ethanol extracts of ten marine macroalgae; two belonging to Chlorophyc... more Diethyl ether, acetone and ethanol extracts of ten marine macroalgae; two belonging to Chlorophyceae (Ulva lactuca and Caulerpa racemosa), two belonging to Rhodophyceae (Acanthophora spicifera and Galaxaura elongata) and six taxa belonging to Phaeophyceae (Liagora farinosa, Cystoseira compressa, Cystoseira myrica, Hydroclathrus clathratus, Turbinaria ornata and Padina pavonia) isolated from the inter tidal zone along Qusier Marsa-Alam seashore (Red Sea), Egypt, were evaluated for their antibacterial, antifungal and antiviral activities against 3 Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus and Sarcina maxima), 3 Gram-negative bacteria (Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumonia), one unicellular (Candida albicans) and two filamentous fungi (Aspergillus flavus and Fusarium oxysporum) and against the Newcastle sense Virus (NDV)-(Paramyxoviridae) which is responsible for acute respiratory distress in chicken. Data showed that some extracts recorded strong inhibitory activities than the reference antibiotics, while others were with moderate and/or week inhibitory activities. However, many were without any inhibitory effects. The cytotoxicity effect of the tested algal extracts on chicken embryo showed that both diethyl ether and acetone extracts had toxic effects, but the ethanol extracts had no toxic effect, so that the ethanol extract was considered to be the most suitable for further studies. The antiviral activities of the ethanol extracts against NDV (Newcastle disease virus) showed that seven of the ten tested algal extracts have strong activities against NDV.
In this study, synthetic and biological soil conditioners were used to screen their abilities to ... more In this study, synthetic and biological soil conditioners were used to screen their abilities to improvise the barren soil characters. These soil conditioners based on treatment of barren soil samples with urea and / or compost as synthetic soil conditioner or by inoculating the soil samples with Spirulina meneghiniana Zanrd. ex Gomon and / or Anabaena oryzae Fritsch as a biological soil conditioner. The data revealed that, the biological conditioner in a mixture (22.5 kg ha -1 Anabaena and 22.5 kg ha -1 Spirulina supplied with 7.5 kg ha -1 urea and 7.5 kg ha -1 compost) was the most effective one. Also, the soil samples inoculated with this mixture exhibited positive activity of improving soil characters. Moreover, highly significant positive responses of the development features were appeared on lettuce plants transplanted in such soil samples.
291 Efficiency of Dunaliella sp. and Aphanocapsa elachista in removing of copper and nickel from ... more 291 Efficiency of Dunaliella sp. and Aphanocapsa elachista in removing of copper and nickel from culture media. Neveen Abdel-Raouf & I.B.M. Ibraheem Botany Department , Faculty of Science, Cairo University (Beni-Suef Branch), BeniSuef, Egypt. Abstract The present investigation evaluated the effectiveness of Dunaliella sp. and Aphanocapsa elachista cells in concentrating copper and nickel in their cells and thereby removing the two metals from solution. Dunaliella sp. (unicellular green alga) was isolated from the 1 st kilometer of Wadi-Sannur (moisted soil with underground water) at the 10 th Kilometer, Eastern-South desert of Beni-Suef Governorate. On the other hand Aphanocapsa elachista (blue-green alga) was isolated from polluted agricultural stream receiving domestic wastewater from Wastewater Treatment Station (WWTS) at Beni-Suef City. The two different taxa were subjected to different concentrations of copper and nickel respectively. It was revealed that Dunaliella sp. was the...
In this study, synthetic and biological soil conditioners were used to screen their abilities to ... more In this study, synthetic and biological soil conditioners were used to screen their abilities to improvise the barren soil characters. These soil conditioners based on treatment of barren soil samples with urea and / or compost as synthetic soil conditioner or by inoculating the soil samples with Spirulina meneghiniana Zanrd. ex Gomon and / or Anabaena oryzae Fritsch as a biological soil conditioner. The data revealed that, the biological conditioner in a mixture (22.5 kg ha-1 Anabaena and 22.5 kg ha-1 Spirulina supplied with 7.5 kg ha-1 urea and 7.5 kg ha-1 compost) was the most effective one. Also, the soil samples inoculated with this mixture exhibited positive activity of improving soil characters. Moreover, highly significant positive responses of the development features were appeared on lettuce plants transplanted in such soil samples.
Ten cyanobacterial species (Nostoc calcicola , N. commune, N. entophytum, N. minutum, N. palndosu... more Ten cyanobacterial species (Nostoc calcicola , N. commune, N. entophytum, N. minutum, N. palndosum, N. passerianum, N. punctiforme, Anabaena ambigua, A. amomala, and A. doliolum) were isolated from the mangrove region of Ras Mohammed (Sinai, Egypt) and have been tested for their allelopathic activity that of inhibitory and / or promoting effects against two Gram positive bacteria (Bacillus subtilis and Staphylococcus aureus) and two Gram negative bacteria (Escherichia coli and Pseudomonas aeruginosa). Data suggested two types of allelopathic effects: one type which always appeared in cyanobacterial medium as in the case with Nostoc minutum (medium that inhibits the growth of all tested bacterial species). The other type is induced only when Cyanobacteria are in contact with bacteria; this is the case when the growth of both Bacillus subtilis and Staphylococcus aureus were inhibited in co-culture with Nostoc commune. On the other hand, promotion effects of bacterial growth were observed when grown in cyanobacterial metabolites in most of studied cyanobacterial species. The biological assays for aqueous and methanolic extracts of the two Nostoc species revealed that both extracts for each species were not toxic at concentrations of 0.52 and 0.59 g L-1 water extract for Nostoc commune and N. minutum, respectively and 0.31 and 0.425 g L-1 for methanolic extract for Nostoc commune and N. minutum, respectively. No mortality was observed in tested mice within 72 hours
I N THIS CONTEXT the brown alga Dictyota dichotoma isolated from Hurghada SeaShore , Red-Sea coas... more I N THIS CONTEXT the brown alga Dictyota dichotoma isolated from Hurghada SeaShore , Red-Sea coast, Egypt (June 2015) was screened for its antimicrobial bioactivity. A laboratory experiment was conducted to test activity of ethanol extract of the brown alga Dictyota dichotoma against three types of Gram-positive and six of Gram-negative bacteria, and one type of fungi. Ethanol extract was selected for this study by using different concentrations (100, 200, 300, 400 mg) of alga extracts. Result revealed that the alga ethanolic extract exhibited high suppression activity against all tested pathogens. HPLC analysis of the chemical composition of algal extract was detect the presence of high amount of certain ingredients such as Flavonoids
M ARINE algae contain a variety of bioactive secondary metabolites and several compounds have bee... more M ARINE algae contain a variety of bioactive secondary metabolites and several compounds have been derived from them for prospective development of novel drugs by the pharmaceutical industries. In this laboratory experiment Cystoseira barbata was isolated from Red Sea coastal water (Safaga, Egypt). It was evaluated due to its bioactivities potential. Where the algal extract proved a potent activity against bacterial and fungal strains ranged between medium and high suppression action. It showed that Gram positive bacteria Bacillus subtilis and Staphylococcus aureus were more sensitive than Gram negative bacteria where Serratia marcescens, Pseudomonas aeuroginosa and fungus Candida albicans. Phyto-chemical analyses showed that C. barbata recorded the highest percentages of the flavonoids, phenols and saccharides compounds. Among the bioflavonoids determined Acacetin, Kaemp.3-(2-pcomaroyl) glucose, Rosmarinic and phenols were E-Vanillic, Benzoic and Ferulic were present in high percentages in the alga analyzed. The results indicated scope for utilizing this alga as a source of antibacterial and antifungal substances.
Beni-Suef University Journal of Basic and Applied Sciences
Abstract In the present investigation, an antihyperlipidemic activity of Cladophora koeiei ethano... more Abstract In the present investigation, an antihyperlipidemic activity of Cladophora koeiei ethanol extract against six pathogenic bacteria was conducted. Also, we evaluate the activity of the alga extract against hyperlipedemia in the administrated albino rates through measuring the blood lipid profiles [triglycerides (TG), total cholesterol (TC), low-density lipoprotein (LDL-C), high-density lipoprotein cholesterol (HDL-C), cholesterol] and induced hepatic damage by measuring the contents of creatinine, total proteins, blood urea nitrogen (BUN), albumin and globulin and diagnostic marker enzymes such as aspartate aminotransaminase (AST) and alanine aminotransaminase (ALT). Alga extract proved efficient activity against the tested bacteria ranged between medium and high suppression action. Results revealed also, the efficiency of C. koeiei extract in the decreasing the triglycerides (TG), total cholesterol (TC), low-density lipoproteins (LDL-C), blood urea nitrogen (BUN) and creatinine caused by alcohol. However, the treatment by alga extract exhibits high-density of lipoproteins (HDL-C) (beneficial), total protein, albumin, and globulins. Also, the algal treatments masking the lethal effects caused by harmful alcohol from raising the rate of enzymes ALT, AST, which returned to the normal state in the groups treated with alga extract. Our findings provide the evidence that new natural antioxidant substances can be present in the C. koeiei extract and hence this alga proves to be effective as a source for therapeutic agents.
Beni-Suef University Journal of Basic and Applied Sciences
Spherical shaped silver nanoparticles were synthesi zed using Fenugreek leaves extract and the pr... more Spherical shaped silver nanoparticles were synthesi zed using Fenugreek leaves extract and the prepared nanoparticles were characterized for their structural, optical and morphological properties. The Fourier transformer infrared spectroscopy reveals the prese nce of polyphenolic compounds in fenugreek leaves e xtract which acts as a reducing agent for the transformati on of silver sulphate into silver nanoparticles. Th e x-ray diffraction pattern substantiate the hexagonal syst em of silver nanoparticles, absence of impurities a scertains the higher order purity of the biosynthesized silver na noparticles. The observed absorption peak at 418nm corresponds to surface plasmon resonance property o f silver nanoparticles. The green synthesized silve r nanoparticles with average size ranging below 50 nm imaged using High Resolution Transmission Electron Microscopy.
Beni-Suef University Journal of Basic and Applied Sciences
Abstract Marine macroalgae are plant-like organisms with simple internal structures that generall... more Abstract Marine macroalgae are plant-like organisms with simple internal structures that generally live in coastal areas. They mainly include different communities of red, brown and green macroalgae. Marine macroalgae commonly occupy intertidal and sublittoral-to-littoral zones on rocks and other hard substrata. They are considered to be an excellent natural biosource in different aspects of agricultural fields. They have great proficiency in improving soil physical and chemical properties. Marine macroalgae are also characterized by producing a large array of biologically active biocidal substances against plant-infecting pathogens. Unfortunately, most available literatures on marine macroalgae and their derivatives mainly focused on their pharmaceutical applications but their potential utilization in sustainable agriculture development is still often regarded as a secondary goal. However, a relatively considerable dataset on marine macroalgae showed that they could play a major role in plant protection and improvement. This review summarizes different aspects of potential macroalgal applications in agriculture. Commercial production and exploitation of specific compounds with interesting biotechnological importance from marine macroalgae including microbicides, nematicides, insecticides, biofertilizers, biostimulators and soil conditioners are highlighted and discussed in detail. Bioactive compounds like fatty acids (in particular polyunsaturated fatty acids (PUFAs), proteins (amino acids), bioflavonoids, sulfated polysaccharides, carotenoids, polyphenols and carbohydrates are considered to have bactericidal, antiviral and fungicidal effects against some plant-infecting pathogens. These biocontrol agents provide multiple benefits and act as useful pointers for improving cultivation practices in diverse habitats. Marine macroalgae can be generally considered as promising multifunctional bioinoculants and ecofriendly environmental tools in recent trends of organic farming.
The hexavalent chromium Cr(VI) poses a threat as a hazardous metal and its removal from aquatic e... more The hexavalent chromium Cr(VI) poses a threat as a hazardous metal and its removal from aquatic environments through biosorption has gained attention as a viable technology of bioremediation. We evaluated the potential use of three green algae (Cladophora glomerata, Enteromorpha intestinalis and Microspora amoena) dry biomass as a biosorbent to remove Cr(VI) from aqueous solutions. The adsorption capacity of the biomass was determined using batch experiments. The adsorption capacity appeared to depend on the pH. The optimum pH with the acid-treated biomass for Cr(VI) biosorption was found to be 2.0 at a constant temperature, 45°C. Among the three genera studied, C. glomerata recorded a maximum of 66.6% removal from the batch process using 1.0 g dried algal cells/100 ml aqueous solution containing an initial concentration of 20 mg/L chromium at 45°C and pH 2.0 for 60 min of contact time. Langmuir and Freundlich isotherm equations fitted to the equilibrium data, Freundlich was the better model. Our study showed that C. glomerata dry biomass is a suitable candidate to remove Cr(VI) from aqueous solutions.
Journal of Pharmaceutical Research International, 2021
Background: Hepatotoxicity was one of the major side effects associated with doxorubicin treatmen... more Background: Hepatotoxicity was one of the major side effects associated with doxorubicin treatment in cancer chemotherapy. The synthesized silver nanoparticles (AgNPs) from natural products such as algae especially green algae is one of the favorable means to minimize the deleterious effects of the chemotherapy. Thus, this study aimed to evaluate the preventive role of AgNPs synthesized by Ulva fasciata (U. fasciata) against doxorubicin-induced hepatotoxicity and oxidative stress in the liver of male Wistar rats. Materials and Methods: In the present study, the green macroalga U. fasciata ethanolic extract was used as reducing agents to reduce Ag ions to Ag0. Doxorubicin-injected male Wistar rats were concomitantly treated with U. fasciata ethanolic extract and AgNPs synthesized by U. fasciata extract (AgNPs/U. fasciata) 3 times/week by oral gavage for 6 weeks. Results: The results showed that male Wistar rats injected with doxorubicin showed a significant increase in ALT, ALP and ...
The aim of this study is to provide the seasonal variations in the coastal macroalgal communities... more The aim of this study is to provide the seasonal variations in the coastal macroalgal communities which inhabiting the upper intertidal zone of Hurghada, Safaga and Qusier coasts at the Red Sea, Egypt. The distribution of algal communities, degree of relative dominance and diversity of the species in different habitats were investigated from autumn 2005 up to summer 2006. Also, the changes in the environmental factors and the abundance of the algal species were correlated. A total of 35 algal species were recorded in the studied area, 7, 7 and 21 species of them belong to Chlorophyta, Rhodophyta and Phaeophyta, respectively. Qusier coast had the highest account of individuals / m (12.71) and total covering (60 %) compared with Hurghada and Safaga coasts. Both Chlorophyta and Rhodophyta were more abundant (66.67 and 48.15 %, respectively) and widely distributed during the summer season along Hurghada and Safaga coasts. Meanwhile, Phaeophyta highest percentage (74.05%) was during autu...
Microalgae sorbents are microalgae that have the potential to passively bind heavy metals/contami... more Microalgae sorbents are microalgae that have the potential to passively bind heavy metals/contaminants to their cellular structures in a process called biosorption. This study investigates the use of two species of microalgae to remove the toxic heavy metal cobalt from aqueous solution. Two microalgae isolates, Phormidium tenue and Chlorella vulgaris, were collected from the Wadi Hanifah Stream in Riyadh, the Kingdom of Saudi Arabia. We determined the capacity of both isolates to bioremove Co+2 ions and the optimum conditions under which this occurs. The two isolates were additionally characterized by microscopic and Fourier transform infrared spectroscopy (FTIR). In the current investigation, Phormidium tenue removed 94% of Co+2 under ideal conditions of pH 6, contact duration (30 min), starting concentration (50 mgL-1) and biosorbent dose (1gL-1); while Chlorella vulgaris removed 87% of Co+2 under the same parameters except pH 5.5 and contact duration (60 min). Fourier transform infrared spectroscopy (FTIR) confirms the binding of Co+2 to the biomass, which comprises many of the functional groups. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed some alterations to the shape of algal cells and cellular components for both microalgae studied. In addition, equilibrium study by both Langmuir and Freundlich models was performed to detect the effect of certain equilibrium factors on the capacity of the biosorption mechanism. Finally, Phormidium tenue and Chlorella vulgaris were discovered to be promising microalgae for effective cobalt biosorption in aquatic conditions.
The exploration of novel therapeutic agents and other bioactive secondary metabolite from Strepto... more The exploration of novel therapeutic agents and other bioactive secondary metabolite from Streptomyces species, for possible agricultural farming, pharmaceutical and industrialized applications, has been, and still is, essential. The existing studies were aimed with biologically potential Streptomyces species and its antagonistic activity against dreadful microorganisms. Totally, morphological three different actinomycetes were selected from the fertile agricultural lands. Among the three, the isolate SA4 exhibited significant antimicrobial and anti-nematicidal activity towards selected microbial pathogens such as E Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, Salmonella typhi, Bacillus subtilis, Proteus vulgaris, Shigella flexineri, Candida albicans, and Fusarium sp. The prospective strain SA4 was identified as Streptomyces cuspidosporus. The isolate SA4 optimized for secondary metabolites production with International Streptomyces project 4 (ISP 4) medium, pH 7.0 at 37°C for 14 days. Gas Chromatography-Mass spectrometry (GCMS) analysis of strain SA4 bioactive extract publicized the existence of 1,2-Benzenedicarboxylic acid, bis(2-Methylpropyl) ester compound and occupied by high peak area and its possessed significant biological properties.
Diethyl ether, acetone and ethanol extracts of ten marine macroalgae; two belonging to Chlorophyc... more Diethyl ether, acetone and ethanol extracts of ten marine macroalgae; two belonging to Chlorophyceae (Ulva lactuca and Caulerpa racemosa), two belonging to Rhodophyceae (Acanthophora spicifera and Galaxaura elongata) and six taxa belonging to Phaeophyceae (Liagora farinosa, Cystoseira compressa, Cystoseira myrica, Hydroclathrus clathratus, Turbinaria ornata and Padina pavonia) isolated from the inter tidal zone along Qusier Marsa-Alam seashore (Red Sea), Egypt, were evaluated for their antibacterial, antifungal and antiviral activities against 3 Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus and Sarcina maxima), 3 Gram-negative bacteria (Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumonia), one unicellular (Candida albicans) and two filamentous fungi (Aspergillus flavus and Fusarium oxysporum) and against the Newcastle sense Virus (NDV)-(Paramyxoviridae) which is responsible for acute respiratory distress in chicken. Data showed that some extracts recorded strong inhibitory activities than the reference antibiotics, while others were with moderate and/or week inhibitory activities. However, many were without any inhibitory effects. The cytotoxicity effect of the tested algal extracts on chicken embryo showed that both diethyl ether and acetone extracts had toxic effects, but the ethanol extracts had no toxic effect, so that the ethanol extract was considered to be the most suitable for further studies. The antiviral activities of the ethanol extracts against NDV (Newcastle disease virus) showed that seven of the ten tested algal extracts have strong activities against NDV.
In this study, synthetic and biological soil conditioners were used to screen their abilities to ... more In this study, synthetic and biological soil conditioners were used to screen their abilities to improvise the barren soil characters. These soil conditioners based on treatment of barren soil samples with urea and / or compost as synthetic soil conditioner or by inoculating the soil samples with Spirulina meneghiniana Zanrd. ex Gomon and / or Anabaena oryzae Fritsch as a biological soil conditioner. The data revealed that, the biological conditioner in a mixture (22.5 kg ha -1 Anabaena and 22.5 kg ha -1 Spirulina supplied with 7.5 kg ha -1 urea and 7.5 kg ha -1 compost) was the most effective one. Also, the soil samples inoculated with this mixture exhibited positive activity of improving soil characters. Moreover, highly significant positive responses of the development features were appeared on lettuce plants transplanted in such soil samples.
291 Efficiency of Dunaliella sp. and Aphanocapsa elachista in removing of copper and nickel from ... more 291 Efficiency of Dunaliella sp. and Aphanocapsa elachista in removing of copper and nickel from culture media. Neveen Abdel-Raouf & I.B.M. Ibraheem Botany Department , Faculty of Science, Cairo University (Beni-Suef Branch), BeniSuef, Egypt. Abstract The present investigation evaluated the effectiveness of Dunaliella sp. and Aphanocapsa elachista cells in concentrating copper and nickel in their cells and thereby removing the two metals from solution. Dunaliella sp. (unicellular green alga) was isolated from the 1 st kilometer of Wadi-Sannur (moisted soil with underground water) at the 10 th Kilometer, Eastern-South desert of Beni-Suef Governorate. On the other hand Aphanocapsa elachista (blue-green alga) was isolated from polluted agricultural stream receiving domestic wastewater from Wastewater Treatment Station (WWTS) at Beni-Suef City. The two different taxa were subjected to different concentrations of copper and nickel respectively. It was revealed that Dunaliella sp. was the...
In this study, synthetic and biological soil conditioners were used to screen their abilities to ... more In this study, synthetic and biological soil conditioners were used to screen their abilities to improvise the barren soil characters. These soil conditioners based on treatment of barren soil samples with urea and / or compost as synthetic soil conditioner or by inoculating the soil samples with Spirulina meneghiniana Zanrd. ex Gomon and / or Anabaena oryzae Fritsch as a biological soil conditioner. The data revealed that, the biological conditioner in a mixture (22.5 kg ha-1 Anabaena and 22.5 kg ha-1 Spirulina supplied with 7.5 kg ha-1 urea and 7.5 kg ha-1 compost) was the most effective one. Also, the soil samples inoculated with this mixture exhibited positive activity of improving soil characters. Moreover, highly significant positive responses of the development features were appeared on lettuce plants transplanted in such soil samples.
Ten cyanobacterial species (Nostoc calcicola , N. commune, N. entophytum, N. minutum, N. palndosu... more Ten cyanobacterial species (Nostoc calcicola , N. commune, N. entophytum, N. minutum, N. palndosum, N. passerianum, N. punctiforme, Anabaena ambigua, A. amomala, and A. doliolum) were isolated from the mangrove region of Ras Mohammed (Sinai, Egypt) and have been tested for their allelopathic activity that of inhibitory and / or promoting effects against two Gram positive bacteria (Bacillus subtilis and Staphylococcus aureus) and two Gram negative bacteria (Escherichia coli and Pseudomonas aeruginosa). Data suggested two types of allelopathic effects: one type which always appeared in cyanobacterial medium as in the case with Nostoc minutum (medium that inhibits the growth of all tested bacterial species). The other type is induced only when Cyanobacteria are in contact with bacteria; this is the case when the growth of both Bacillus subtilis and Staphylococcus aureus were inhibited in co-culture with Nostoc commune. On the other hand, promotion effects of bacterial growth were observed when grown in cyanobacterial metabolites in most of studied cyanobacterial species. The biological assays for aqueous and methanolic extracts of the two Nostoc species revealed that both extracts for each species were not toxic at concentrations of 0.52 and 0.59 g L-1 water extract for Nostoc commune and N. minutum, respectively and 0.31 and 0.425 g L-1 for methanolic extract for Nostoc commune and N. minutum, respectively. No mortality was observed in tested mice within 72 hours
I N THIS CONTEXT the brown alga Dictyota dichotoma isolated from Hurghada SeaShore , Red-Sea coas... more I N THIS CONTEXT the brown alga Dictyota dichotoma isolated from Hurghada SeaShore , Red-Sea coast, Egypt (June 2015) was screened for its antimicrobial bioactivity. A laboratory experiment was conducted to test activity of ethanol extract of the brown alga Dictyota dichotoma against three types of Gram-positive and six of Gram-negative bacteria, and one type of fungi. Ethanol extract was selected for this study by using different concentrations (100, 200, 300, 400 mg) of alga extracts. Result revealed that the alga ethanolic extract exhibited high suppression activity against all tested pathogens. HPLC analysis of the chemical composition of algal extract was detect the presence of high amount of certain ingredients such as Flavonoids
M ARINE algae contain a variety of bioactive secondary metabolites and several compounds have bee... more M ARINE algae contain a variety of bioactive secondary metabolites and several compounds have been derived from them for prospective development of novel drugs by the pharmaceutical industries. In this laboratory experiment Cystoseira barbata was isolated from Red Sea coastal water (Safaga, Egypt). It was evaluated due to its bioactivities potential. Where the algal extract proved a potent activity against bacterial and fungal strains ranged between medium and high suppression action. It showed that Gram positive bacteria Bacillus subtilis and Staphylococcus aureus were more sensitive than Gram negative bacteria where Serratia marcescens, Pseudomonas aeuroginosa and fungus Candida albicans. Phyto-chemical analyses showed that C. barbata recorded the highest percentages of the flavonoids, phenols and saccharides compounds. Among the bioflavonoids determined Acacetin, Kaemp.3-(2-pcomaroyl) glucose, Rosmarinic and phenols were E-Vanillic, Benzoic and Ferulic were present in high percentages in the alga analyzed. The results indicated scope for utilizing this alga as a source of antibacterial and antifungal substances.
Beni-Suef University Journal of Basic and Applied Sciences
Abstract In the present investigation, an antihyperlipidemic activity of Cladophora koeiei ethano... more Abstract In the present investigation, an antihyperlipidemic activity of Cladophora koeiei ethanol extract against six pathogenic bacteria was conducted. Also, we evaluate the activity of the alga extract against hyperlipedemia in the administrated albino rates through measuring the blood lipid profiles [triglycerides (TG), total cholesterol (TC), low-density lipoprotein (LDL-C), high-density lipoprotein cholesterol (HDL-C), cholesterol] and induced hepatic damage by measuring the contents of creatinine, total proteins, blood urea nitrogen (BUN), albumin and globulin and diagnostic marker enzymes such as aspartate aminotransaminase (AST) and alanine aminotransaminase (ALT). Alga extract proved efficient activity against the tested bacteria ranged between medium and high suppression action. Results revealed also, the efficiency of C. koeiei extract in the decreasing the triglycerides (TG), total cholesterol (TC), low-density lipoproteins (LDL-C), blood urea nitrogen (BUN) and creatinine caused by alcohol. However, the treatment by alga extract exhibits high-density of lipoproteins (HDL-C) (beneficial), total protein, albumin, and globulins. Also, the algal treatments masking the lethal effects caused by harmful alcohol from raising the rate of enzymes ALT, AST, which returned to the normal state in the groups treated with alga extract. Our findings provide the evidence that new natural antioxidant substances can be present in the C. koeiei extract and hence this alga proves to be effective as a source for therapeutic agents.
Beni-Suef University Journal of Basic and Applied Sciences
Spherical shaped silver nanoparticles were synthesi zed using Fenugreek leaves extract and the pr... more Spherical shaped silver nanoparticles were synthesi zed using Fenugreek leaves extract and the prepared nanoparticles were characterized for their structural, optical and morphological properties. The Fourier transformer infrared spectroscopy reveals the prese nce of polyphenolic compounds in fenugreek leaves e xtract which acts as a reducing agent for the transformati on of silver sulphate into silver nanoparticles. Th e x-ray diffraction pattern substantiate the hexagonal syst em of silver nanoparticles, absence of impurities a scertains the higher order purity of the biosynthesized silver na noparticles. The observed absorption peak at 418nm corresponds to surface plasmon resonance property o f silver nanoparticles. The green synthesized silve r nanoparticles with average size ranging below 50 nm imaged using High Resolution Transmission Electron Microscopy.
Beni-Suef University Journal of Basic and Applied Sciences
Abstract Marine macroalgae are plant-like organisms with simple internal structures that generall... more Abstract Marine macroalgae are plant-like organisms with simple internal structures that generally live in coastal areas. They mainly include different communities of red, brown and green macroalgae. Marine macroalgae commonly occupy intertidal and sublittoral-to-littoral zones on rocks and other hard substrata. They are considered to be an excellent natural biosource in different aspects of agricultural fields. They have great proficiency in improving soil physical and chemical properties. Marine macroalgae are also characterized by producing a large array of biologically active biocidal substances against plant-infecting pathogens. Unfortunately, most available literatures on marine macroalgae and their derivatives mainly focused on their pharmaceutical applications but their potential utilization in sustainable agriculture development is still often regarded as a secondary goal. However, a relatively considerable dataset on marine macroalgae showed that they could play a major role in plant protection and improvement. This review summarizes different aspects of potential macroalgal applications in agriculture. Commercial production and exploitation of specific compounds with interesting biotechnological importance from marine macroalgae including microbicides, nematicides, insecticides, biofertilizers, biostimulators and soil conditioners are highlighted and discussed in detail. Bioactive compounds like fatty acids (in particular polyunsaturated fatty acids (PUFAs), proteins (amino acids), bioflavonoids, sulfated polysaccharides, carotenoids, polyphenols and carbohydrates are considered to have bactericidal, antiviral and fungicidal effects against some plant-infecting pathogens. These biocontrol agents provide multiple benefits and act as useful pointers for improving cultivation practices in diverse habitats. Marine macroalgae can be generally considered as promising multifunctional bioinoculants and ecofriendly environmental tools in recent trends of organic farming.
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