Journal of Chemical Process and Material Technology
5-Fluorouracil is a commonly used chemotherapeutic agent in patients with colon cancer. The side ... more 5-Fluorouracil is a commonly used chemotherapeutic agent in patients with colon cancer. The side effect using of 5-fluorouracil such as cardiotoxicity and immunosupressioncan lead to death. One of the approach to overcome overloaded use of 5-fluorouracil is the combination with a chemopreventive agent, including the grape seeds extract (Vitis vinifera L.). This research aims to reviewing the effect of grape seeds extract on the cytotoxic activity of 5-fluorouracil in modulating cell cycle and apoptosis induction of colon cancer cellsWiDr. Determination of the cytotoxic activity of grape seeds extract and 5-fluorouracil as well as a combination of both conducted by MTT assay. Modulation surveillance of cell cycle and apoptosis induction is done by using flowcytometry and analyzed by FACS Calibur program. Cytotoxicity assay single treatment of grape seeds extract (IC50) is 403,957μg/ml, whereas IC50 values 5-fluorouracil is 848 µM. Observations modulation of cell cycle and apoptosis...
ABSTRAK Jantung pisang mas mengandung senyawa yang dapat menangkap radikal bebas antara lain sapo... more ABSTRAK Jantung pisang mas mengandung senyawa yang dapat menangkap radikal bebas antara lain saponin, fenol, flavonoid. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antioksidan ekstrak etanol, fraksi etil asetat, dan fraksi air jantung pisang mas (Musa acuminata Colla) dengan metode DPPH (2,2-difenil-1-pikrilhidrazil). Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etanol 70%, dilanjutkan fraksinasi cair-cair menggunakan pelarut etil asetat dan air. Penentuan aktivitas antioksidan ekstrak etanol dan fraksi air dilakukan pada seri konsentrasi 5, 10, 15, 20, dan 25 µg/ml. Sedangkan untuk fraksi etil asetat digunakan konsentrasi sebesar 4, 8, 12, 16, dan 20 µg/ml. Persentase aktivitas antioksidan diperoleh dari data absorbansi. Nilai IC50 didapatkan dari regresi linier antara konsentrasi larutan seri uji dengan persentase aktivitas antioksidan. Identifikasi senyawa flavonoid dilakukan dengan metode KLT. Hasil penelitian menunjukkan bahwa ekstrak etanol dan...
Doxorubicin is chemotherapeutic agents used widely for breast cancer therapy. The use of doxorubi... more Doxorubicin is chemotherapeutic agents used widely for breast cancer therapy. The use of doxorubicin may cause cardio toxicity and multi-drug resistance. In order to overcome this side effect, a combined therapy using the methanol extracts of kenikir leaves is developed. The purposes of this study are to measure the cytotoxic effect of methanol extracts of kenikir leaves and doxorubicin combination treatment against T47D breast cancer cell growth in-vitro and in-silico, as well as determining the optimal dose combination that produces the most potent cytotoxic effects. The extraction of kenikir leaves is performed with soxhletation method. The cytotoxicity assay on the T47D breast cancer cells is carried out using MTT assay. The result of this research shows that the value of IC50 methanol extracts of kenikir leaves is 778.05μg/mL. The treatment of the methanol extracts of kenikir leaves and doxorubicin combination treatment results in a strong synergy at the concentration 130 μg/mL...
Ethyl p-methoxycinnamate and ethyl cinnamate as a phytoestrogen in galangal root (Kaempferia gala... more Ethyl p-methoxycinnamate and ethyl cinnamate as a phytoestrogen in galangal root (Kaempferia galanga L.) expected to be used for replacing the Hormone Replacement Therapy on prevention osteoporosis and as anti-cholesterol in menopausal women. The purpose of this study is to explore the potential estrogenic effect of galangal root extract on prevention of osteoporosis and as an anti-cholesterol agent. In-silico test is performed to measure the molecular interactions between ethyl p-methoxycinnamate and ethyl cinnamate with estrogen receptor protein (1QKM) and in-vivo tests conducted by the ovariectomy method on female Wistar rats. Thirty female Wistar rats were divided into six treatment groups. Group I is the normal mice and the group II-VI is the ovariectomies rats. Ovariectomy surgery performed at 70 days of age rats. Group II is the baseline of ovariectomized rats. Group III rats treated with CMC-Na 0.5% (control). Group IV rats are treated with estradiol 2 μg/kg BW/day while the...
Breast cancer is one of the most deadly cancers. The main priority of breast cancer treatment is ... more Breast cancer is one of the most deadly cancers. The main priority of breast cancer treatment is the use of chemotheraupetic agents, especially doxorubicin. However, breast cancer treatment using doxorubicin has potency to generate cardiotoxicity and cancer cell resistance. An approach recently developed to overcome side effects of doxorubicin is to used of combined chemotheraupetic agent (cochemotherapeutic agent), i.e. ursolic acid. The aim of this research is to develop the potency of ursolic acid as cochemotherapeutic agent by observing the cytotoxic effect and cell cycle modulation on MCF-7 breast cancer cell lines. The determination of the cytotoxic activity of ursolic acid and doxorubicin and the combination of them was done using MTT assay to obtain the value of IC50 and CI (Combination Index). The profile of cell cycle is observed using flowcytometry and analyzed using Modfit LT 3.0 program. Ursolic acid had cytotoxic effect on MCF-7 breast cancer cell with IC50 of 37 μM. The combination ursolic acid ½ IC50 (19 μM) and doxorubicin ¼ IC50 (87,5 nM), 1/3 IC50 (116,7 nM), and ½ IC50 (175 nM
Common Cold is a resporatory disturbance symptom which well-marked by cough, sneez, clogged up no... more Common Cold is a resporatory disturbance symptom which well-marked by cough, sneez, clogged up nose, painful red lane, fever, and headache. Nowadays, people tend to have self medication to cope with Common Cold by consuming free medicines and limited free medicines which sold freely in market. The aim in this research was to evaluate Common Cold medicines utilizat ion by people in Karanggondang Mlonggo Jepara. This research is a non-experimental study. Data was obtained from field survey by using quetionnaire that have been filled by people in Karanggondang Mlonggo Jepara. The result in this research have being analized by using descriptive non analytical method. Sample was taken proportionally with convenient sampling method. This research showed that description of self medicat ion on Karanggondang Mlonggo Jepara peple was sufferer frequency 40,25% then action that have been taken by Common Cold sufferer who tried to do self medication 77,75%, their reason to do self medication ba...
Cervical uterine cancer is a deadly tumor attacking the cervix uteri. In Indonesia, cervix uteri ... more Cervical uterine cancer is a deadly tumor attacking the cervix uteri. In Indonesia, cervix uteri cancer constitutes the most aggresive and deadly cancer. Frequently, combinations of several different drugs are used for treating this disease. The purpose of this study was to find out the stastisticts and kind of drug interactions hospitalized in Dr. Kariadi Hospital Semarang for cervix uteri cancer in 2007. This study was a non-experimental research conducted retrospectively. The research was implemented to the medical records for cervix uteri cancer patients hospitalized in Dr. Kariadi Hospital Semarang. Data obtained were analysed with descriptive non-analytic method. The result of this study showed that 96 in-patients of cervix uteri cancer, 55 patients (57,29 %) had drug interactions, while 41 patients (42,7 %) had not drug interactions. From a total number of 149 cases, 134 cases (89,93 %) had drug interaction, while 15 cases (10,07 %) had not drug interaction. Based on the type...
5-Fluorouracil is a commonly used chemotherapeutic agent in patients with colon cancer. The risk ... more 5-Fluorouracil is a commonly used chemotherapeutic agent in patients with colon cancer. The risk of side effect using of 5-fluorouracil such as cardiotoxicity and immunosupression which can lead to death. One of the approach to overcome overloaded use of 5-fluorouracil is the combined use with a chemopreventive agent (co-chemotherapy), including the seeds extract of grape (Vitis vinifera L.). This research aims to reviewing the effect of the methanol extract of seeds of grape on the cytotoxic activity of 5-fluorouracil in modulating cell cycle and apoptosis of colon cancer cells WiDr. Determination of the cytotoxic activity of methanol extract of seeds of grape and 5-fluorouracil as well as a combination of both conducted by MTT assay. Modulation surveillance of cell cycle and apoptosis induction is done by using flowcytometry and analyzed by FACS Calibur program. Cytotoxicity assay single treatment of the methanol extract of seeds of grape produce use values of IC50 403,957 μg/m...
Jantung pisang mas mengandung senyawa yang dapat menangkap radikal bebas antara lain saponin, fen... more Jantung pisang mas mengandung senyawa yang dapat menangkap radikal bebas antara lain saponin, fenol, flavonoid. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antioksidan ekstrak etanol, fraksi etil asetat, dan fraksi air jantung pisang mas (Musa acuminata Colla) dengan metode DPPH (2,2-difenil-1-pikrilhidrazil). Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etanol 70%, dilanjutkan fraksinasi cair-cair menggunakan pelarut etil asetat dan air. Penentuan aktivitas antioksidan ekstrak etanol dan fraksi air dilakukan pada seri konsentrasi 5, 10, 15, 20, dan 25 μg/ml. Sedangkan untuk fraksi etil asetat digunakan konsentrasi sebesar 4, 8, 12, 16, dan 20 μg/ml. Persentase aktivitas antioksidan diperoleh dari data absorbansi. Nilai IC50 didapatkan dari regresi linier antara konsentrasi larutan seri uji dengan persentase aktivitas antioksidan. Identifikasi senyawa flavonoid dilakukan dengan metode KLT. Hasil penelitian menunjukkan bahwa ekstrak etanol dan fraksi ...
Breast and cervical cancers were the high prevalency, both in the world and Indonesia. This study... more Breast and cervical cancers were the high prevalency, both in the world and Indonesia. This study aimed to determine the cytotoxic activities and its potentcy of n-hexane fraction of ethanol extract alfalfa herb (Medicago sativa L.) in T47D cells and HeLa cells, also identificated its chemical compounds. Extraction was done using ethanol by soxhletation method and then followed fractionation with n-hexane. Cytotoxic assay to get the value of IC50 was carried out by using MTT assay with a series of concentration of fraction 62,5; 125; 250; 500; 1000 μg/ ml. The cytotoxic activities was determined with viability cells from absorbance ELISA reader. Identification of chemical compounds of n-hexane fraction of ethanol extract alfalfa herb was done using chemical reactan and paper chromatography and then was evaluated qualitatively. The results showed that the n-hexane fraction of ethanol extract of alfalfa herb had cytotoxic activities in T47D cells and HeLa cells. The IC50 values were 5...
ABSTRAK Seiring dengan peningkatan penggunaan madu, berkembanglah pemalsuan madu untuk mendapatka... more ABSTRAK Seiring dengan peningkatan penggunaan madu, berkembanglah pemalsuan madu untuk mendapatkan keuntungan. Penambahan larutan gula pasir sering dilakukan karena harganya yang relatif murah. Pemalsuan madu dengan cara seperti ini sangat merugikan masyarakat karena dapat memicu timbulnya penyakit diabetes. Kandungan sukrosa dalam madu menurut persyaratan mutu madu SNI 01-3545-2004 adalah maksimal 5%. Apabila kandungannya jauh lebih tinggi maka dapat dipastikan bahwa madu tersebut tidak asli. Metode didasarkan pada sifat glukosa yang polar sehingga dapat dianalisis menggunakan KCKT dan struktur glukosa yang mengandung kromofor sehingga dapat dideteksi menggunakan detektor UV. Analisis dilakukan dengan kolom C 18 Lichrospher 100 RP-18 (100 mm x 4.6 mm ID, 5 μm). Fase gerak terdiri atas asetonitril dan aquabidest (65: 35, v/v). Fase gerak dihantarkan secara isokratik dengan laju alir 0,8 mL/menit. Detektor UV pada panjang gelombang 191 nm. Metode yang dikembangkan divalidasi berdasar...
Kanker payudara dan kanker leher rahim merupakan jenis penyakit ganas di Indonesia maupun dunia.... more Kanker payudara dan kanker leher rahim merupakan jenis penyakit ganas di Indonesia maupun dunia. Penelitian ini bertujuan untuk mengetahui potensi sitotoksik fraksi etil asetat dari ekstrak etanol herba alfalfa terhadap sel kanker payudara (sel T47D) dan sel kanker leher rahim (sel HeLa) serta mengetahui kandungan kimianya. Proses ekstraksi herba alfalfa dilakukan dengan metode sokletasi menggunakan pelarut etanol kemudian dilanjutkan fraksinasi secara bertingkat menggunakan n -heksan, kloroform, dietil eter dan etil asetat. Uji sitotoksisitas menggunakan metode MTT dengan seri konsentrasi fraksi uji 1000; 500; 250; 125; 62,5 µg/ml terhadap kultur sel T47D dan sel HeLa. Data berupa absorbansi sel hidup digunakan untuk menghitung persentase kehidupan sel T47D dan sel HeLa kemudian ditetapkan IC 50 dengan analisis probit menggunakan SPSS 16 for Windows . Uji kandungan kimia dilakukan dengan pereaksi kimia kemudian dilanjutkan dengan kromatografi lapis tipis (KLT). Hasil penelitian...
Ocimum basilicum L has proven to have in vitro cytotoxic activity against breast cancer cells. Pa... more Ocimum basilicum L has proven to have in vitro cytotoxic activity against breast cancer cells. Pathways that cause cell death can involve one of the proteins, which is BCL-2. This study aims to determine the decrease of BCL-2 protein expressions in breast cancer cells (T47D and MCF-7) tat are treated with the ethanol extract of Ocimum basilicum L. Ocimum basilicum L. was extracted using the maceration method with 70% ethanol solvent. The concentration of ethanol extract of Ocimum basilicum L. used to see the expression of BCL-2 protein in T47D and MCF-7 cells was 199 µg/ml and 388 µg / mL. The observation of BCL-2 protein expression is using immunocytochemical methods of T47D and MCF-7 cancer cells. The results showed that the ethanol extract of Ocimum basilicum L could reduce BCL-2 protein expression in breast cancer cells (T47D and MCF-7) at concentrations of 199 µg/ml and 388 µg/ml, respectively.
Doxorubicin is a commonly used chemotherapeutic agent in patients with breast cancer. The risk of... more Doxorubicin is a commonly used chemotherapeutic agent in patients with breast cancer. The risk of side effect using of doxorubicin such as cardiomyopathy and congestive heart failure which can lead to death. One of the approach to overcome overloaded use of doxorubicin is the combined use with a chemopreventive agent (cochemotherapy), including the leaf extract of marigolds (Cosmos caudatus Kunth.). This research aims to reviewing the effect of the methanol extract of leaves of marigolds on the cytotoxic activity of doxorubicin in modulating cell cycle and apoptosis of breast cancer cells T47D. Determination of the cytotoxic activity of methanol extract of leaves of marigolds and doxorubicin as well as a combination of both conducted by MTT assay. modulation surveillance of cell cycle and apoptosis induction is done by using flowcytometry and analyzed by FACS Calibur program. Cytotoxicity assay single treatment of the methanol extract of leaves of marigolds produce use values of I...
ABSTRAK Doksorubisin adalah agen kemoterapi yang mempunyai efek samping hepatotoksik dengan cara ... more ABSTRAK Doksorubisin adalah agen kemoterapi yang mempunyai efek samping hepatotoksik dengan cara pembentukan radikal bebas. Daun kenikir (Cosmos caudatus Kunth.) memiliki potensi untuk mencegah efek hepatotoksik akibat penggunaan doksorubisin. Penelitian bertujian untuk membuktikan potensi Ekstrak Etanol Daun Kenikir (EEDK) sebagai hepatoprotektor pada tikus yang diinduksi doksorubisin. Penelitian ini merupakan penelitian eksperimental menggunakan randomized matched post only control group design. Sebanyak 25 ekor tikus galur wistar dibagi menjadi 5 kelompok secara acak. Kelompok I, control normal diberi asupan aquadest, kelompok II kontrol hepatotoksik diberikan doksorubisin dosis 5 mg/KgBB. Kelompok III, IV dan V merupakan kelompok perlakuan EEDK dengan dosis berturut-turut 250,500, dan 1000 mg/kgBB dengan doksorubisin dosis 5 mg/kgBB. Pemberian doksorubisin dilakukan pada hari ke-1, ke-5,ke-9 dan hari ke-13 secara intraperitonial (ip) dan pemberian EEDK dilakukan 1 kali sehari se...
ABSTRAK Pengobatan kanker menggunakan bahan alam terus dikembangkan. Salah satu tanaman yang memi... more ABSTRAK Pengobatan kanker menggunakan bahan alam terus dikembangkan. Salah satu tanaman yang memiliki efek sitotoksik Ocimum basilicum, L. tujuan penelitian ini adalah mengetahui aktivitas sitotoksik dari ekstrak etanol Ocimum basilicum (EEOB) terhadap sel kanker payudara T47D dan MCF7. Ocimum basilicum diekstraksi menggunakan metode maserasi dengan pelarut etanol 70%. Pengujian aktivitas sitotoksik menggunakan metode MTT assay dengan seri konsentrasi EEOB 1000; 500; 250; 125; 62.5; 31,25 µg/mL. Hasil uji aktivitas sitotoksik EEOB memperlihatkan nilai IC50 pada sel T47D dan MCF-7 sebesar 399.86 µg/ml dan 387.76 µg/ml. Kata Kunci—Sitotoksik, Ocinum basilicum L., T47D, MCF-7 ABSTRACT Cancer treatment using natural ingredients continues to be developed. One of the plants that is proven to have cytotoxic activity is basil leaves (Ocimum basilicum, L.). This study aims to determine the cytotoxic activity of ethanol extract of basil leaves (EEBL) on T47D and MCF-7 breast can...
Journal of Chemical Process and Material Technology
5-Fluorouracil is a commonly used chemotherapeutic agent in patients with colon cancer. The side ... more 5-Fluorouracil is a commonly used chemotherapeutic agent in patients with colon cancer. The side effect using of 5-fluorouracil such as cardiotoxicity and immunosupressioncan lead to death. One of the approach to overcome overloaded use of 5-fluorouracil is the combination with a chemopreventive agent, including the grape seeds extract (Vitis vinifera L.). This research aims to reviewing the effect of grape seeds extract on the cytotoxic activity of 5-fluorouracil in modulating cell cycle and apoptosis induction of colon cancer cellsWiDr. Determination of the cytotoxic activity of grape seeds extract and 5-fluorouracil as well as a combination of both conducted by MTT assay. Modulation surveillance of cell cycle and apoptosis induction is done by using flowcytometry and analyzed by FACS Calibur program. Cytotoxicity assay single treatment of grape seeds extract (IC50) is 403,957μg/ml, whereas IC50 values 5-fluorouracil is 848 µM. Observations modulation of cell cycle and apoptosis...
ABSTRAK Jantung pisang mas mengandung senyawa yang dapat menangkap radikal bebas antara lain sapo... more ABSTRAK Jantung pisang mas mengandung senyawa yang dapat menangkap radikal bebas antara lain saponin, fenol, flavonoid. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antioksidan ekstrak etanol, fraksi etil asetat, dan fraksi air jantung pisang mas (Musa acuminata Colla) dengan metode DPPH (2,2-difenil-1-pikrilhidrazil). Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etanol 70%, dilanjutkan fraksinasi cair-cair menggunakan pelarut etil asetat dan air. Penentuan aktivitas antioksidan ekstrak etanol dan fraksi air dilakukan pada seri konsentrasi 5, 10, 15, 20, dan 25 µg/ml. Sedangkan untuk fraksi etil asetat digunakan konsentrasi sebesar 4, 8, 12, 16, dan 20 µg/ml. Persentase aktivitas antioksidan diperoleh dari data absorbansi. Nilai IC50 didapatkan dari regresi linier antara konsentrasi larutan seri uji dengan persentase aktivitas antioksidan. Identifikasi senyawa flavonoid dilakukan dengan metode KLT. Hasil penelitian menunjukkan bahwa ekstrak etanol dan...
Doxorubicin is chemotherapeutic agents used widely for breast cancer therapy. The use of doxorubi... more Doxorubicin is chemotherapeutic agents used widely for breast cancer therapy. The use of doxorubicin may cause cardio toxicity and multi-drug resistance. In order to overcome this side effect, a combined therapy using the methanol extracts of kenikir leaves is developed. The purposes of this study are to measure the cytotoxic effect of methanol extracts of kenikir leaves and doxorubicin combination treatment against T47D breast cancer cell growth in-vitro and in-silico, as well as determining the optimal dose combination that produces the most potent cytotoxic effects. The extraction of kenikir leaves is performed with soxhletation method. The cytotoxicity assay on the T47D breast cancer cells is carried out using MTT assay. The result of this research shows that the value of IC50 methanol extracts of kenikir leaves is 778.05μg/mL. The treatment of the methanol extracts of kenikir leaves and doxorubicin combination treatment results in a strong synergy at the concentration 130 μg/mL...
Ethyl p-methoxycinnamate and ethyl cinnamate as a phytoestrogen in galangal root (Kaempferia gala... more Ethyl p-methoxycinnamate and ethyl cinnamate as a phytoestrogen in galangal root (Kaempferia galanga L.) expected to be used for replacing the Hormone Replacement Therapy on prevention osteoporosis and as anti-cholesterol in menopausal women. The purpose of this study is to explore the potential estrogenic effect of galangal root extract on prevention of osteoporosis and as an anti-cholesterol agent. In-silico test is performed to measure the molecular interactions between ethyl p-methoxycinnamate and ethyl cinnamate with estrogen receptor protein (1QKM) and in-vivo tests conducted by the ovariectomy method on female Wistar rats. Thirty female Wistar rats were divided into six treatment groups. Group I is the normal mice and the group II-VI is the ovariectomies rats. Ovariectomy surgery performed at 70 days of age rats. Group II is the baseline of ovariectomized rats. Group III rats treated with CMC-Na 0.5% (control). Group IV rats are treated with estradiol 2 μg/kg BW/day while the...
Breast cancer is one of the most deadly cancers. The main priority of breast cancer treatment is ... more Breast cancer is one of the most deadly cancers. The main priority of breast cancer treatment is the use of chemotheraupetic agents, especially doxorubicin. However, breast cancer treatment using doxorubicin has potency to generate cardiotoxicity and cancer cell resistance. An approach recently developed to overcome side effects of doxorubicin is to used of combined chemotheraupetic agent (cochemotherapeutic agent), i.e. ursolic acid. The aim of this research is to develop the potency of ursolic acid as cochemotherapeutic agent by observing the cytotoxic effect and cell cycle modulation on MCF-7 breast cancer cell lines. The determination of the cytotoxic activity of ursolic acid and doxorubicin and the combination of them was done using MTT assay to obtain the value of IC50 and CI (Combination Index). The profile of cell cycle is observed using flowcytometry and analyzed using Modfit LT 3.0 program. Ursolic acid had cytotoxic effect on MCF-7 breast cancer cell with IC50 of 37 μM. The combination ursolic acid ½ IC50 (19 μM) and doxorubicin ¼ IC50 (87,5 nM), 1/3 IC50 (116,7 nM), and ½ IC50 (175 nM
Common Cold is a resporatory disturbance symptom which well-marked by cough, sneez, clogged up no... more Common Cold is a resporatory disturbance symptom which well-marked by cough, sneez, clogged up nose, painful red lane, fever, and headache. Nowadays, people tend to have self medication to cope with Common Cold by consuming free medicines and limited free medicines which sold freely in market. The aim in this research was to evaluate Common Cold medicines utilizat ion by people in Karanggondang Mlonggo Jepara. This research is a non-experimental study. Data was obtained from field survey by using quetionnaire that have been filled by people in Karanggondang Mlonggo Jepara. The result in this research have being analized by using descriptive non analytical method. Sample was taken proportionally with convenient sampling method. This research showed that description of self medicat ion on Karanggondang Mlonggo Jepara peple was sufferer frequency 40,25% then action that have been taken by Common Cold sufferer who tried to do self medication 77,75%, their reason to do self medication ba...
Cervical uterine cancer is a deadly tumor attacking the cervix uteri. In Indonesia, cervix uteri ... more Cervical uterine cancer is a deadly tumor attacking the cervix uteri. In Indonesia, cervix uteri cancer constitutes the most aggresive and deadly cancer. Frequently, combinations of several different drugs are used for treating this disease. The purpose of this study was to find out the stastisticts and kind of drug interactions hospitalized in Dr. Kariadi Hospital Semarang for cervix uteri cancer in 2007. This study was a non-experimental research conducted retrospectively. The research was implemented to the medical records for cervix uteri cancer patients hospitalized in Dr. Kariadi Hospital Semarang. Data obtained were analysed with descriptive non-analytic method. The result of this study showed that 96 in-patients of cervix uteri cancer, 55 patients (57,29 %) had drug interactions, while 41 patients (42,7 %) had not drug interactions. From a total number of 149 cases, 134 cases (89,93 %) had drug interaction, while 15 cases (10,07 %) had not drug interaction. Based on the type...
5-Fluorouracil is a commonly used chemotherapeutic agent in patients with colon cancer. The risk ... more 5-Fluorouracil is a commonly used chemotherapeutic agent in patients with colon cancer. The risk of side effect using of 5-fluorouracil such as cardiotoxicity and immunosupression which can lead to death. One of the approach to overcome overloaded use of 5-fluorouracil is the combined use with a chemopreventive agent (co-chemotherapy), including the seeds extract of grape (Vitis vinifera L.). This research aims to reviewing the effect of the methanol extract of seeds of grape on the cytotoxic activity of 5-fluorouracil in modulating cell cycle and apoptosis of colon cancer cells WiDr. Determination of the cytotoxic activity of methanol extract of seeds of grape and 5-fluorouracil as well as a combination of both conducted by MTT assay. Modulation surveillance of cell cycle and apoptosis induction is done by using flowcytometry and analyzed by FACS Calibur program. Cytotoxicity assay single treatment of the methanol extract of seeds of grape produce use values of IC50 403,957 μg/m...
Jantung pisang mas mengandung senyawa yang dapat menangkap radikal bebas antara lain saponin, fen... more Jantung pisang mas mengandung senyawa yang dapat menangkap radikal bebas antara lain saponin, fenol, flavonoid. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antioksidan ekstrak etanol, fraksi etil asetat, dan fraksi air jantung pisang mas (Musa acuminata Colla) dengan metode DPPH (2,2-difenil-1-pikrilhidrazil). Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etanol 70%, dilanjutkan fraksinasi cair-cair menggunakan pelarut etil asetat dan air. Penentuan aktivitas antioksidan ekstrak etanol dan fraksi air dilakukan pada seri konsentrasi 5, 10, 15, 20, dan 25 μg/ml. Sedangkan untuk fraksi etil asetat digunakan konsentrasi sebesar 4, 8, 12, 16, dan 20 μg/ml. Persentase aktivitas antioksidan diperoleh dari data absorbansi. Nilai IC50 didapatkan dari regresi linier antara konsentrasi larutan seri uji dengan persentase aktivitas antioksidan. Identifikasi senyawa flavonoid dilakukan dengan metode KLT. Hasil penelitian menunjukkan bahwa ekstrak etanol dan fraksi ...
Breast and cervical cancers were the high prevalency, both in the world and Indonesia. This study... more Breast and cervical cancers were the high prevalency, both in the world and Indonesia. This study aimed to determine the cytotoxic activities and its potentcy of n-hexane fraction of ethanol extract alfalfa herb (Medicago sativa L.) in T47D cells and HeLa cells, also identificated its chemical compounds. Extraction was done using ethanol by soxhletation method and then followed fractionation with n-hexane. Cytotoxic assay to get the value of IC50 was carried out by using MTT assay with a series of concentration of fraction 62,5; 125; 250; 500; 1000 μg/ ml. The cytotoxic activities was determined with viability cells from absorbance ELISA reader. Identification of chemical compounds of n-hexane fraction of ethanol extract alfalfa herb was done using chemical reactan and paper chromatography and then was evaluated qualitatively. The results showed that the n-hexane fraction of ethanol extract of alfalfa herb had cytotoxic activities in T47D cells and HeLa cells. The IC50 values were 5...
ABSTRAK Seiring dengan peningkatan penggunaan madu, berkembanglah pemalsuan madu untuk mendapatka... more ABSTRAK Seiring dengan peningkatan penggunaan madu, berkembanglah pemalsuan madu untuk mendapatkan keuntungan. Penambahan larutan gula pasir sering dilakukan karena harganya yang relatif murah. Pemalsuan madu dengan cara seperti ini sangat merugikan masyarakat karena dapat memicu timbulnya penyakit diabetes. Kandungan sukrosa dalam madu menurut persyaratan mutu madu SNI 01-3545-2004 adalah maksimal 5%. Apabila kandungannya jauh lebih tinggi maka dapat dipastikan bahwa madu tersebut tidak asli. Metode didasarkan pada sifat glukosa yang polar sehingga dapat dianalisis menggunakan KCKT dan struktur glukosa yang mengandung kromofor sehingga dapat dideteksi menggunakan detektor UV. Analisis dilakukan dengan kolom C 18 Lichrospher 100 RP-18 (100 mm x 4.6 mm ID, 5 μm). Fase gerak terdiri atas asetonitril dan aquabidest (65: 35, v/v). Fase gerak dihantarkan secara isokratik dengan laju alir 0,8 mL/menit. Detektor UV pada panjang gelombang 191 nm. Metode yang dikembangkan divalidasi berdasar...
Kanker payudara dan kanker leher rahim merupakan jenis penyakit ganas di Indonesia maupun dunia.... more Kanker payudara dan kanker leher rahim merupakan jenis penyakit ganas di Indonesia maupun dunia. Penelitian ini bertujuan untuk mengetahui potensi sitotoksik fraksi etil asetat dari ekstrak etanol herba alfalfa terhadap sel kanker payudara (sel T47D) dan sel kanker leher rahim (sel HeLa) serta mengetahui kandungan kimianya. Proses ekstraksi herba alfalfa dilakukan dengan metode sokletasi menggunakan pelarut etanol kemudian dilanjutkan fraksinasi secara bertingkat menggunakan n -heksan, kloroform, dietil eter dan etil asetat. Uji sitotoksisitas menggunakan metode MTT dengan seri konsentrasi fraksi uji 1000; 500; 250; 125; 62,5 µg/ml terhadap kultur sel T47D dan sel HeLa. Data berupa absorbansi sel hidup digunakan untuk menghitung persentase kehidupan sel T47D dan sel HeLa kemudian ditetapkan IC 50 dengan analisis probit menggunakan SPSS 16 for Windows . Uji kandungan kimia dilakukan dengan pereaksi kimia kemudian dilanjutkan dengan kromatografi lapis tipis (KLT). Hasil penelitian...
Ocimum basilicum L has proven to have in vitro cytotoxic activity against breast cancer cells. Pa... more Ocimum basilicum L has proven to have in vitro cytotoxic activity against breast cancer cells. Pathways that cause cell death can involve one of the proteins, which is BCL-2. This study aims to determine the decrease of BCL-2 protein expressions in breast cancer cells (T47D and MCF-7) tat are treated with the ethanol extract of Ocimum basilicum L. Ocimum basilicum L. was extracted using the maceration method with 70% ethanol solvent. The concentration of ethanol extract of Ocimum basilicum L. used to see the expression of BCL-2 protein in T47D and MCF-7 cells was 199 µg/ml and 388 µg / mL. The observation of BCL-2 protein expression is using immunocytochemical methods of T47D and MCF-7 cancer cells. The results showed that the ethanol extract of Ocimum basilicum L could reduce BCL-2 protein expression in breast cancer cells (T47D and MCF-7) at concentrations of 199 µg/ml and 388 µg/ml, respectively.
Doxorubicin is a commonly used chemotherapeutic agent in patients with breast cancer. The risk of... more Doxorubicin is a commonly used chemotherapeutic agent in patients with breast cancer. The risk of side effect using of doxorubicin such as cardiomyopathy and congestive heart failure which can lead to death. One of the approach to overcome overloaded use of doxorubicin is the combined use with a chemopreventive agent (cochemotherapy), including the leaf extract of marigolds (Cosmos caudatus Kunth.). This research aims to reviewing the effect of the methanol extract of leaves of marigolds on the cytotoxic activity of doxorubicin in modulating cell cycle and apoptosis of breast cancer cells T47D. Determination of the cytotoxic activity of methanol extract of leaves of marigolds and doxorubicin as well as a combination of both conducted by MTT assay. modulation surveillance of cell cycle and apoptosis induction is done by using flowcytometry and analyzed by FACS Calibur program. Cytotoxicity assay single treatment of the methanol extract of leaves of marigolds produce use values of I...
ABSTRAK Doksorubisin adalah agen kemoterapi yang mempunyai efek samping hepatotoksik dengan cara ... more ABSTRAK Doksorubisin adalah agen kemoterapi yang mempunyai efek samping hepatotoksik dengan cara pembentukan radikal bebas. Daun kenikir (Cosmos caudatus Kunth.) memiliki potensi untuk mencegah efek hepatotoksik akibat penggunaan doksorubisin. Penelitian bertujian untuk membuktikan potensi Ekstrak Etanol Daun Kenikir (EEDK) sebagai hepatoprotektor pada tikus yang diinduksi doksorubisin. Penelitian ini merupakan penelitian eksperimental menggunakan randomized matched post only control group design. Sebanyak 25 ekor tikus galur wistar dibagi menjadi 5 kelompok secara acak. Kelompok I, control normal diberi asupan aquadest, kelompok II kontrol hepatotoksik diberikan doksorubisin dosis 5 mg/KgBB. Kelompok III, IV dan V merupakan kelompok perlakuan EEDK dengan dosis berturut-turut 250,500, dan 1000 mg/kgBB dengan doksorubisin dosis 5 mg/kgBB. Pemberian doksorubisin dilakukan pada hari ke-1, ke-5,ke-9 dan hari ke-13 secara intraperitonial (ip) dan pemberian EEDK dilakukan 1 kali sehari se...
ABSTRAK Pengobatan kanker menggunakan bahan alam terus dikembangkan. Salah satu tanaman yang memi... more ABSTRAK Pengobatan kanker menggunakan bahan alam terus dikembangkan. Salah satu tanaman yang memiliki efek sitotoksik Ocimum basilicum, L. tujuan penelitian ini adalah mengetahui aktivitas sitotoksik dari ekstrak etanol Ocimum basilicum (EEOB) terhadap sel kanker payudara T47D dan MCF7. Ocimum basilicum diekstraksi menggunakan metode maserasi dengan pelarut etanol 70%. Pengujian aktivitas sitotoksik menggunakan metode MTT assay dengan seri konsentrasi EEOB 1000; 500; 250; 125; 62.5; 31,25 µg/mL. Hasil uji aktivitas sitotoksik EEOB memperlihatkan nilai IC50 pada sel T47D dan MCF-7 sebesar 399.86 µg/ml dan 387.76 µg/ml. Kata Kunci—Sitotoksik, Ocinum basilicum L., T47D, MCF-7 ABSTRACT Cancer treatment using natural ingredients continues to be developed. One of the plants that is proven to have cytotoxic activity is basil leaves (Ocimum basilicum, L.). This study aims to determine the cytotoxic activity of ethanol extract of basil leaves (EEBL) on T47D and MCF-7 breast can...
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