Marine Biologist. University of ChileResearch Biologist - Department of Fisheries and Oceans - Canada (1983 - 1998).Professor, Fisheries and Aquaculture. Faculty of Fisheries and Oceanography, University Austral de Chile.Salmon Aquaculture Consultant - Fish Reproductive physiology, genetics and selective breeding.
ABSTRACT Groups of monosex female eyed eggs and newly hatched alevins received single immersions ... more ABSTRACT Groups of monosex female eyed eggs and newly hatched alevins received single immersions in 17α-methyltestosterone at 20 or 200 μg/l for 30, 60 or 120 min at 10°C. Three groups received a second immersion treatment 8 days later at 200 μg/l for 30, 60 or 120 min. The fish were sampled at 122 days post-hatch and the results indicated that all the treatments affected gonadal morphology. The most significant effect was at 200 μg/l where 82–100% males were produced as compared to 0% males in the monosex female control group. The percentage of males was increased by double immersion treatments. An increase in the treatment duration at 20 μg/l also increased the percentage of males. Survival was high in all groups and the mean weights of the treated groups were larger than the control group at 122 days post-hatch. This study demonstrates that the sex differentiation of chinook salmon is very labile and easily influenced by androgen treatment around the time of hatching. A simplified technique for producing phenotypic males consists of two 2-h immersions at 200 μg/l 17α-methyltestosterone in incubation water of 10°C, 1 week apart, commencing during final hatching.
Abstract Heat shocks were applied to wild rainbow trout eggs for 10 min, starting at 1 and 40 min... more Abstract Heat shocks were applied to wild rainbow trout eggs for 10 min, starting at 1 and 40 min after fertilization and at temperatures ranging from 24 to 30 C. Analysis of blood samples by flow cytometry revealed triploid induction ranging from 10 to 100% depending on the ...
The Reproductive Containment of Genetically Altered Salmonids EDWARD M. DONALDSON, ROBERT H. DEVL... more The Reproductive Containment of Genetically Altered Salmonids EDWARD M. DONALDSON, ROBERT H. DEVLIN, IGOR I. SOLAR, AND FRANCESC PIFERRER ... Total physical containment is not feasible in lake or ocean based net pens; however, recent concern regarding the ...
... 7 00 5T Fig. 1. Effect of oral administration of 17a-methyltestosterone on sex differentiatio... more ... 7 00 5T Fig. 1. Effect of oral administration of 17a-methyltestosterone on sex differentiation (gonadal morphology) of rainbow trout treated for periods of 60, 90 and 120 days. with 50 mg/kg diet for 120 days. Two-way ANOVA ...
... John's, p. 134. RE Withler et al. Aquaculture 136 (1995) 91107 107 Lincoln, RF and Scott,... more ... John's, p. 134. RE Withler et al. Aquaculture 136 (1995) 91107 107 Lincoln, RF and Scott, AP, 1984. Sexual maturation in triploid rainbow trout, Saimo gairdneri Richardson. J. Fish Biol., 25: 385392. McLean, E., Sadar, MD, Devlin, RH, Souza, LM and Donaldson, EM, 1991. ...
This chapter describes and reviews existing and potential methods of confinement of transgenic fi... more This chapter describes and reviews existing and potential methods of confinement of transgenic fish. The technical aspects of physical confinement measures that are necessary and appropriate to minimize the possibility of the entry of transgenic fish into a receiving environment are presented. Geographic confinement is discussed, with reference to cases where it has been applied at a research level. A range of technologies that provide variable levels of biological confinement are presented.
... all-female strains of chinook salmon Robert H. Devlin*, B. Kelly McNeil, Igor 1. Solar, Edwar... more ... all-female strains of chinook salmon Robert H. Devlin*, B. Kelly McNeil, Igor 1. Solar, Edward M. Donaldson Fisheries and Oceans Canada, West Vancouver Laboratory ... Such differences may be observed in a high proportion of younger fish as well (Beacham and Murray, 1986). ...
The delivery of maturational hormones to fish, via injection, or by implantation of slow-release ... more The delivery of maturational hormones to fish, via injection, or by implantation of slow-release devices has been successfully employed by the aquaculture industry to induce ovulation in various species of teleost (reviewed by Donaldson and Hunter 1983). However, handling, and a variety of other factors, may inhibit the ovarian response and cause follicular atresia in fish (reviewed by Billard et al. 1981). Thus, an effective method of inducing ovulation without the need of physical manipulation would be extremely valuable to the ...
Hydrostatic pressure or thermal shocks were administered to coho salmon eggs 20, 40, 60 and 80 mi... more Hydrostatic pressure or thermal shocks were administered to coho salmon eggs 20, 40, 60 and 80 min post-insemination. Pressure shocks ranged from 6.2 to 8.4×104 kPa, and lasted 4 min. Thermal shocks ranged from 24 to 28°C, and were applied for durations of 10 and 20 min. Triploid induction rate for each treatment group was evaluated by measurement of erythrocyte DNA content using flow cytometric analysis. Triploid rates of 100% were produced by pressure shocks of 6.9–8.3×104 kPa for eggs 20 min post-fertilization and by thermal trauma of 28°C for 20 min, 20 min after fertilization. Survival was inversely correlated with the intensity of treatments. Thus, triploid yields calculated as the product of the triploid rate and the numbers surviving to hatch, expressed as a percentage of the number of eggs originally present in the groups, were highest with a hydrostatic pressure treatment of 6.2×104 kPa for 4 min treatment, 20 and 40 min after fertilization or a heat shock of 26°C for 20 min treatment, 20 min after fertilization.
Transactions of the American Fisheries Society, Nov 1, 1986
Transactions of the American Fisheries Society 115:838-840, 1986 ... Flow-Cytometric Confirmation... more Transactions of the American Fisheries Society 115:838-840, 1986 ... Flow-Cytometric Confirmation of Aneuploidy in Sperm from Triploid Rainbow Trout ... TILLMANN J. BENFEY AND IGOR I. SOLAR Department of Fisheries and Oceans, Fisheries Research Branch West Vancouver ...
Http Dx Doi Org 10 1577 1548 8659115 2 0 Co 2, Jan 9, 2011
Transactions of the American Fisheries Society 115:838-840, 1986 ... Flow-Cytometric Confirmation... more Transactions of the American Fisheries Society 115:838-840, 1986 ... Flow-Cytometric Confirmation of Aneuploidy in Sperm from Triploid Rainbow Trout ... TILLMANN J. BENFEY AND IGOR I. SOLAR Department of Fisheries and Oceans, Fisheries Research Branch West Vancouver ...
... Lyne Morissette, Simon-Pierre Despatie, Claude Savenkoff Mike O. Hammill, Hugo Bourdages, and... more ... Lyne Morissette, Simon-Pierre Despatie, Claude Savenkoff Mike O. Hammill, Hugo Bourdages, and Denis Chabot Department of Fisheries and Oceans ... by Lyne Morissette1, Simon-Pierre Despatie, Claude Savenkoff, Mike O. Hammill, Hugo Bourdages, and Denis Chabot ...
Hydrostatic pressure or thermal shocks were administered to coho salmon eggs 20, 40, 60 and 80 mi... more Hydrostatic pressure or thermal shocks were administered to coho salmon eggs 20, 40, 60 and 80 min post-insemination. Pressure shocks ranged from 6.2 to 8.4× 10 4 kPa, and lasted 4 min. Thermal shocks ranged from 24 to 28 C, and were applied for durations of 10 and 20 min. Triploid induction rate for each treatment group was evaluated by measurement of erythrocyte DNA content using flow cytometric analysis. Triploid rates of 100% were produced by pressure shocks of 6.9–8.3× 10 4 kPa for eggs 20 min post- ...
ABSTRACT Groups of monosex female eyed eggs and newly hatched alevins received single immersions ... more ABSTRACT Groups of monosex female eyed eggs and newly hatched alevins received single immersions in 17α-methyltestosterone at 20 or 200 μg/l for 30, 60 or 120 min at 10°C. Three groups received a second immersion treatment 8 days later at 200 μg/l for 30, 60 or 120 min. The fish were sampled at 122 days post-hatch and the results indicated that all the treatments affected gonadal morphology. The most significant effect was at 200 μg/l where 82–100% males were produced as compared to 0% males in the monosex female control group. The percentage of males was increased by double immersion treatments. An increase in the treatment duration at 20 μg/l also increased the percentage of males. Survival was high in all groups and the mean weights of the treated groups were larger than the control group at 122 days post-hatch. This study demonstrates that the sex differentiation of chinook salmon is very labile and easily influenced by androgen treatment around the time of hatching. A simplified technique for producing phenotypic males consists of two 2-h immersions at 200 μg/l 17α-methyltestosterone in incubation water of 10°C, 1 week apart, commencing during final hatching.
Abstract Heat shocks were applied to wild rainbow trout eggs for 10 min, starting at 1 and 40 min... more Abstract Heat shocks were applied to wild rainbow trout eggs for 10 min, starting at 1 and 40 min after fertilization and at temperatures ranging from 24 to 30 C. Analysis of blood samples by flow cytometry revealed triploid induction ranging from 10 to 100% depending on the ...
The Reproductive Containment of Genetically Altered Salmonids EDWARD M. DONALDSON, ROBERT H. DEVL... more The Reproductive Containment of Genetically Altered Salmonids EDWARD M. DONALDSON, ROBERT H. DEVLIN, IGOR I. SOLAR, AND FRANCESC PIFERRER ... Total physical containment is not feasible in lake or ocean based net pens; however, recent concern regarding the ...
... 7 00 5T Fig. 1. Effect of oral administration of 17a-methyltestosterone on sex differentiatio... more ... 7 00 5T Fig. 1. Effect of oral administration of 17a-methyltestosterone on sex differentiation (gonadal morphology) of rainbow trout treated for periods of 60, 90 and 120 days. with 50 mg/kg diet for 120 days. Two-way ANOVA ...
... John's, p. 134. RE Withler et al. Aquaculture 136 (1995) 91107 107 Lincoln, RF and Scott,... more ... John's, p. 134. RE Withler et al. Aquaculture 136 (1995) 91107 107 Lincoln, RF and Scott, AP, 1984. Sexual maturation in triploid rainbow trout, Saimo gairdneri Richardson. J. Fish Biol., 25: 385392. McLean, E., Sadar, MD, Devlin, RH, Souza, LM and Donaldson, EM, 1991. ...
This chapter describes and reviews existing and potential methods of confinement of transgenic fi... more This chapter describes and reviews existing and potential methods of confinement of transgenic fish. The technical aspects of physical confinement measures that are necessary and appropriate to minimize the possibility of the entry of transgenic fish into a receiving environment are presented. Geographic confinement is discussed, with reference to cases where it has been applied at a research level. A range of technologies that provide variable levels of biological confinement are presented.
... all-female strains of chinook salmon Robert H. Devlin*, B. Kelly McNeil, Igor 1. Solar, Edwar... more ... all-female strains of chinook salmon Robert H. Devlin*, B. Kelly McNeil, Igor 1. Solar, Edward M. Donaldson Fisheries and Oceans Canada, West Vancouver Laboratory ... Such differences may be observed in a high proportion of younger fish as well (Beacham and Murray, 1986). ...
The delivery of maturational hormones to fish, via injection, or by implantation of slow-release ... more The delivery of maturational hormones to fish, via injection, or by implantation of slow-release devices has been successfully employed by the aquaculture industry to induce ovulation in various species of teleost (reviewed by Donaldson and Hunter 1983). However, handling, and a variety of other factors, may inhibit the ovarian response and cause follicular atresia in fish (reviewed by Billard et al. 1981). Thus, an effective method of inducing ovulation without the need of physical manipulation would be extremely valuable to the ...
Hydrostatic pressure or thermal shocks were administered to coho salmon eggs 20, 40, 60 and 80 mi... more Hydrostatic pressure or thermal shocks were administered to coho salmon eggs 20, 40, 60 and 80 min post-insemination. Pressure shocks ranged from 6.2 to 8.4×104 kPa, and lasted 4 min. Thermal shocks ranged from 24 to 28°C, and were applied for durations of 10 and 20 min. Triploid induction rate for each treatment group was evaluated by measurement of erythrocyte DNA content using flow cytometric analysis. Triploid rates of 100% were produced by pressure shocks of 6.9–8.3×104 kPa for eggs 20 min post-fertilization and by thermal trauma of 28°C for 20 min, 20 min after fertilization. Survival was inversely correlated with the intensity of treatments. Thus, triploid yields calculated as the product of the triploid rate and the numbers surviving to hatch, expressed as a percentage of the number of eggs originally present in the groups, were highest with a hydrostatic pressure treatment of 6.2×104 kPa for 4 min treatment, 20 and 40 min after fertilization or a heat shock of 26°C for 20 min treatment, 20 min after fertilization.
Transactions of the American Fisheries Society, Nov 1, 1986
Transactions of the American Fisheries Society 115:838-840, 1986 ... Flow-Cytometric Confirmation... more Transactions of the American Fisheries Society 115:838-840, 1986 ... Flow-Cytometric Confirmation of Aneuploidy in Sperm from Triploid Rainbow Trout ... TILLMANN J. BENFEY AND IGOR I. SOLAR Department of Fisheries and Oceans, Fisheries Research Branch West Vancouver ...
Http Dx Doi Org 10 1577 1548 8659115 2 0 Co 2, Jan 9, 2011
Transactions of the American Fisheries Society 115:838-840, 1986 ... Flow-Cytometric Confirmation... more Transactions of the American Fisheries Society 115:838-840, 1986 ... Flow-Cytometric Confirmation of Aneuploidy in Sperm from Triploid Rainbow Trout ... TILLMANN J. BENFEY AND IGOR I. SOLAR Department of Fisheries and Oceans, Fisheries Research Branch West Vancouver ...
... Lyne Morissette, Simon-Pierre Despatie, Claude Savenkoff Mike O. Hammill, Hugo Bourdages, and... more ... Lyne Morissette, Simon-Pierre Despatie, Claude Savenkoff Mike O. Hammill, Hugo Bourdages, and Denis Chabot Department of Fisheries and Oceans ... by Lyne Morissette1, Simon-Pierre Despatie, Claude Savenkoff, Mike O. Hammill, Hugo Bourdages, and Denis Chabot ...
Hydrostatic pressure or thermal shocks were administered to coho salmon eggs 20, 40, 60 and 80 mi... more Hydrostatic pressure or thermal shocks were administered to coho salmon eggs 20, 40, 60 and 80 min post-insemination. Pressure shocks ranged from 6.2 to 8.4× 10 4 kPa, and lasted 4 min. Thermal shocks ranged from 24 to 28 C, and were applied for durations of 10 and 20 min. Triploid induction rate for each treatment group was evaluated by measurement of erythrocyte DNA content using flow cytometric analysis. Triploid rates of 100% were produced by pressure shocks of 6.9–8.3× 10 4 kPa for eggs 20 min post- ...
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