Ratiometric fluorescent nanothermometers with near‐infrared emission play an important role in in... more Ratiometric fluorescent nanothermometers with near‐infrared emission play an important role in in vivo sensing since they can be used as intracellular thermal sensing probes with high spatial resolution and high sensitivity, to investigate cellular functions of interest in diagnosis and therapy, where current approaches are not effective. Herein, the temperature‐dependent fluorescence of organic nanoparticles is designed, synthesized, and studied based on the dual emission, generated by monomer and excimer species, of the tris(2,4,6‐trichlorophenyl)methyl radical (TTM) doping organic nanoparticles (TTMd‐ONPs), made of optically neutral tris(2,4,6‐trichlorophenyl)methane (TTM‐αH), acting as a matrix. The excimer emission intensity of TTMd‐ONPs decreases with increasing temperatures whereas the monomer emission is almost independent and can be used as an internal reference. TTMd‐ONPs show a great temperature sensitivity (3.4% K−1 at 328 K) and a wide temperature response at ambient co...
Biofunctionalized surfaces with an antimicrobial multidomain protein both in soluble form or nano... more Biofunctionalized surfaces with an antimicrobial multidomain protein both in soluble form or nanostructured as nanoparticles to avoid biofilm formation.
Antibiotic resistance has exponentially increased during the last years. It is necessary to devel... more Antibiotic resistance has exponentially increased during the last years. It is necessary to develop new antimicrobial drugs to prevent and treat infectious diseases caused by multidrug- or extensively-drug resistant (MDR/XDR)-bacteria. Host Defense Peptides (HDPs) have a versatile role, acting as antimicrobial peptides and regulators of several innate immunity functions. The results shown by previous studies using synthetic HDPs are only the tip of the iceberg, since the synergistic potential of HDPs and their production as recombinant proteins are fields practically unexplored. The present study aims to move a step forward through the development of a new generation of tailored antimicrobials, using a rational design of recombinant multidomain proteins based on HDPs. This strategy is based on a two-phase process, starting with the construction of the first generation molecules using single HDPs and further selecting those HDPs with higher bactericidal efficiencies to be combined in...
Lymph node-inspired 3D hydrogels with precisely defined porosity were produced, which improve the... more Lymph node-inspired 3D hydrogels with precisely defined porosity were produced, which improve the state-of-the-art T cell proliferation, a procedure that is especially important for novel cellular immunotherapies.
Mesoporous materials feature ordered tailored structures with uniform pore sizes and highly acces... more Mesoporous materials feature ordered tailored structures with uniform pore sizes and highly accessible surface areas, making them an ideal host for functional organic molecules or nanoparticles for analytical and sensing applications. Moreover, as their porosity could be employed to deliver fluids, they could be suitable materials for nanofluidic devices. As a first step in this direction, we present a study of the hydration of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) model lipid membranes on solid mesoporous support. POPC was selected as it changes the structure upon hydration at room temperature. Mesoporous films were prepared using two different templating agents, Pluronic P123 (PEO–PPO–PEO triblock copolymer where PEO is polyethylene oxide and PPO is polypropylene oxide) and Brij 58 (C16H33(EO)20OH where EO is ethylene oxide), both following the conventional route and by X-ray irradiation via deep X-ray lithography technique and subsequent development. The same sa...
A particular fluorene-derivative-basedo-carborane π-conjugated system exhibits a strong solid-sta... more A particular fluorene-derivative-basedo-carborane π-conjugated system exhibits a strong solid-state luminescence efficiency, while preserving the luminescent properties as nanoparticles homogeneously dispersed in water.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
The electron donor-acceptor tetrathiafulvalene-polychlorotriphenylmethyl (PTM) radical dyad, whic... more The electron donor-acceptor tetrathiafulvalene-polychlorotriphenylmethyl (PTM) radical dyad, which shows a strong interplay between intra- and intermolecular charge transfer processes in solution, has been deposited by drop-casting on highly oriented pyrolytic graphite substrates, and its self-assembled structure has been investigated. Conducting atomic force microscopy revealed that the presence of a PTM radical in the molecules enhances the electrical conduction by almost two orders of magnitude and that this enhancement occurs in spite of the poor molecular orientation control achieved with drop-casting. Moreover, the study also reveals that the presence of a tetrathiafulvalene subunit in the deposited molecules can result in slightly asymmetric I-V curves.
Diketopyrrolopyrroles (DPPs) have recently attracted much interest as very bright and photostable... more Diketopyrrolopyrroles (DPPs) have recently attracted much interest as very bright and photostable red‐emitting molecules. However, their tendency to form nonfluorescent aggregates in water through the aggregation‐caused quenching (ACQ) effect is a major issue that limits their application under the microscope. Herein, two DPP molecules have been incorporated into the membrane of highly stable and water‐soluble quatsomes (QS; nanovesicles composed of surfactants and sterols), which allow their nanostructuration in water and, at the same time, limits the ACQ effect. The obtained fluorescent organic nanoparticles showed superior structural homogeneity, along with long‐term colloidal and optical stability. A thorough one‐ (1P) and two‐photon (2P) fluorescence characterization revealed the promising photophysical features of these fluorescent nanovesicles, which showed a high 1P and 2P brightness. Finally, the fluorescent QSs were used for the in vitro bioimaging of Saos‐2 osteosarcoma c...
Engineering new materials which are capable of trapping biomolecules in nanoscale quantities, is ... more Engineering new materials which are capable of trapping biomolecules in nanoscale quantities, is crucial in order to achieve earlier diagnostics in different diseases.
We describe the modification of the Work Function (WF) of Au(111) upon deposition of Self-Assembl... more We describe the modification of the Work Function (WF) of Au(111) upon deposition of Self-Assembled Monolayers (SAMs) with two donor-acceptor (D-A) systems, one based on a ferrocene-polychlorotriphenylmethyl radical (Fc-PTM) dyad...
Ratiometric fluorescent nanothermometers with near‐infrared emission play an important role in in... more Ratiometric fluorescent nanothermometers with near‐infrared emission play an important role in in vivo sensing since they can be used as intracellular thermal sensing probes with high spatial resolution and high sensitivity, to investigate cellular functions of interest in diagnosis and therapy, where current approaches are not effective. Herein, the temperature‐dependent fluorescence of organic nanoparticles is designed, synthesized, and studied based on the dual emission, generated by monomer and excimer species, of the tris(2,4,6‐trichlorophenyl)methyl radical (TTM) doping organic nanoparticles (TTMd‐ONPs), made of optically neutral tris(2,4,6‐trichlorophenyl)methane (TTM‐αH), acting as a matrix. The excimer emission intensity of TTMd‐ONPs decreases with increasing temperatures whereas the monomer emission is almost independent and can be used as an internal reference. TTMd‐ONPs show a great temperature sensitivity (3.4% K−1 at 328 K) and a wide temperature response at ambient co...
Biofunctionalized surfaces with an antimicrobial multidomain protein both in soluble form or nano... more Biofunctionalized surfaces with an antimicrobial multidomain protein both in soluble form or nanostructured as nanoparticles to avoid biofilm formation.
Antibiotic resistance has exponentially increased during the last years. It is necessary to devel... more Antibiotic resistance has exponentially increased during the last years. It is necessary to develop new antimicrobial drugs to prevent and treat infectious diseases caused by multidrug- or extensively-drug resistant (MDR/XDR)-bacteria. Host Defense Peptides (HDPs) have a versatile role, acting as antimicrobial peptides and regulators of several innate immunity functions. The results shown by previous studies using synthetic HDPs are only the tip of the iceberg, since the synergistic potential of HDPs and their production as recombinant proteins are fields practically unexplored. The present study aims to move a step forward through the development of a new generation of tailored antimicrobials, using a rational design of recombinant multidomain proteins based on HDPs. This strategy is based on a two-phase process, starting with the construction of the first generation molecules using single HDPs and further selecting those HDPs with higher bactericidal efficiencies to be combined in...
Lymph node-inspired 3D hydrogels with precisely defined porosity were produced, which improve the... more Lymph node-inspired 3D hydrogels with precisely defined porosity were produced, which improve the state-of-the-art T cell proliferation, a procedure that is especially important for novel cellular immunotherapies.
Mesoporous materials feature ordered tailored structures with uniform pore sizes and highly acces... more Mesoporous materials feature ordered tailored structures with uniform pore sizes and highly accessible surface areas, making them an ideal host for functional organic molecules or nanoparticles for analytical and sensing applications. Moreover, as their porosity could be employed to deliver fluids, they could be suitable materials for nanofluidic devices. As a first step in this direction, we present a study of the hydration of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) model lipid membranes on solid mesoporous support. POPC was selected as it changes the structure upon hydration at room temperature. Mesoporous films were prepared using two different templating agents, Pluronic P123 (PEO–PPO–PEO triblock copolymer where PEO is polyethylene oxide and PPO is polypropylene oxide) and Brij 58 (C16H33(EO)20OH where EO is ethylene oxide), both following the conventional route and by X-ray irradiation via deep X-ray lithography technique and subsequent development. The same sa...
A particular fluorene-derivative-basedo-carborane π-conjugated system exhibits a strong solid-sta... more A particular fluorene-derivative-basedo-carborane π-conjugated system exhibits a strong solid-state luminescence efficiency, while preserving the luminescent properties as nanoparticles homogeneously dispersed in water.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
The electron donor-acceptor tetrathiafulvalene-polychlorotriphenylmethyl (PTM) radical dyad, whic... more The electron donor-acceptor tetrathiafulvalene-polychlorotriphenylmethyl (PTM) radical dyad, which shows a strong interplay between intra- and intermolecular charge transfer processes in solution, has been deposited by drop-casting on highly oriented pyrolytic graphite substrates, and its self-assembled structure has been investigated. Conducting atomic force microscopy revealed that the presence of a PTM radical in the molecules enhances the electrical conduction by almost two orders of magnitude and that this enhancement occurs in spite of the poor molecular orientation control achieved with drop-casting. Moreover, the study also reveals that the presence of a tetrathiafulvalene subunit in the deposited molecules can result in slightly asymmetric I-V curves.
Diketopyrrolopyrroles (DPPs) have recently attracted much interest as very bright and photostable... more Diketopyrrolopyrroles (DPPs) have recently attracted much interest as very bright and photostable red‐emitting molecules. However, their tendency to form nonfluorescent aggregates in water through the aggregation‐caused quenching (ACQ) effect is a major issue that limits their application under the microscope. Herein, two DPP molecules have been incorporated into the membrane of highly stable and water‐soluble quatsomes (QS; nanovesicles composed of surfactants and sterols), which allow their nanostructuration in water and, at the same time, limits the ACQ effect. The obtained fluorescent organic nanoparticles showed superior structural homogeneity, along with long‐term colloidal and optical stability. A thorough one‐ (1P) and two‐photon (2P) fluorescence characterization revealed the promising photophysical features of these fluorescent nanovesicles, which showed a high 1P and 2P brightness. Finally, the fluorescent QSs were used for the in vitro bioimaging of Saos‐2 osteosarcoma c...
Engineering new materials which are capable of trapping biomolecules in nanoscale quantities, is ... more Engineering new materials which are capable of trapping biomolecules in nanoscale quantities, is crucial in order to achieve earlier diagnostics in different diseases.
We describe the modification of the Work Function (WF) of Au(111) upon deposition of Self-Assembl... more We describe the modification of the Work Function (WF) of Au(111) upon deposition of Self-Assembled Monolayers (SAMs) with two donor-acceptor (D-A) systems, one based on a ferrocene-polychlorotriphenylmethyl radical (Fc-PTM) dyad...
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