Background: Most materials used clinically for filling severe bone defects either cannot induce b... more Background: Most materials used clinically for filling severe bone defects either cannot induce bone re-generation or exhibit low bone conversion, therefore, their therapeutic effects are limited. Human umbilical cord mesenchymal stem cells (hUC-MSCs) exhibit good osteoinduction. However, the mechanism by which combining a heterogeneous bone collagen matrix with hUC-MSCs to repair the bone defects of alveolar process clefts remains unclear.Methods: A rabbit alveolar process cleft model was established by removing the bone tissue from the left maxillary bone. 48 young Japanese white rabbits (JWRs) were divided into normal, control, material and MSCs groups. An equal volume of a bone collagen matrix alone or combined with hUC-MSCs was implanted in the defect. X-ray, micro-focus computed tomography (micro-CT), blood analysis, histochemical staining and TUNEL were used to detect the newly formed bone in the defect area at 3 and 6 months after the surgery. Results: The bone formation rat...
Background: Most materials used clinically for filling severe bone defects lack the ability to in... more Background: Most materials used clinically for filling severe bone defects lack the ability to induce bone regeneration or low bone conversion rate, therefore, their therapeutic effects are limited. Human umbilical cord mesenchymal stem cells (HUC-MSCs) have good osteoinduction. The mechanism of heterogeneous bone collagen matrix combined with HUC-MSCs in repairing bone defects of alveolar process cleft is still unclear.Methods: Here, a rabbit alveolar process cleft model was established to evaluate the repair effect and molecular mechanism of bone collagen matrix and its combination with HUC-MSCs on critical-sized bone defects in the alveolar process. We randomly divided 48 young Japanese white rabbits (JWRs) into normal, control, material and MSCs groups. The alveolar process cleft model was established by removing the bone of equal volume at the left maxillary. Bone collagen matrix combined with HUC-MSCs that were then implanted in the defect area. X-ray analysis and blood analys...
Background: Most materials used clinically for filling severe bone defects either cannot induce b... more Background: Most materials used clinically for filling severe bone defects either cannot induce bone re-generation or exhibit low bone conversion, therefore, their therapeutic effects are limited. Human umbilical cord mesenchymal stem cells (hUC-MSCs) exhibit good osteoinduction. However, the mechanism by which combining a heterogeneous bone collagen matrix with hUC-MSCs to repair the bone defects of alveolar process clefts remains unclear.Methods: A rabbit alveolar process cleft model was established by removing the bone tissue from the left maxillary bone. 48 young Japanese white rabbits (JWRs) were divided into normal, control, material and MSCs groups. An equal volume of a bone collagen matrix alone or combined with hUC-MSCs was implanted in the defect. X-ray, micro-focus computed tomography (micro-CT), blood analysis, histochemical staining and TUNEL were used to detect the newly formed bone in the defect area at 3 and 6 months after the surgery. Results: The bone formation rat...
Background: Most materials used clinically for filling severe bone defects lack the ability to in... more Background: Most materials used clinically for filling severe bone defects lack the ability to induce bone regeneration or low bone conversion rate, therefore, their therapeutic effects are limited. Human umbilical cord mesenchymal stem cells (HUC-MSCs) have good osteoinduction. The mechanism of heterogeneous bone collagen matrix combined with HUC-MSCs in repairing bone defects of alveolar process cleft is still unclear.Methods: Here, a rabbit alveolar process cleft model was established to evaluate the repair effect and molecular mechanism of bone collagen matrix and its combination with HUC-MSCs on critical-sized bone defects in the alveolar process. We randomly divided 48 young Japanese white rabbits (JWRs) into normal, control, material and MSCs groups. The alveolar process cleft model was established by removing the bone of equal volume at the left maxillary. Bone collagen matrix combined with HUC-MSCs that were then implanted in the defect area. X-ray analysis and blood analys...
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Papers by Ismaela Silva