To investigate the feasibility of bone turnover markers (BTMs) for the assessment of bone metabol... more To investigate the feasibility of bone turnover markers (BTMs) for the assessment of bone metabolism in patients with systemic lupus erythematosus (SLE), according to the guidelines of the International Osteoporosis Foundation and the International Federation of Clinical Chemistry and Laboratory Medicine. The study included 43 female SLE patients. Serum procollagen type I N propeptide (PINP), C-terminal telopeptide of type I collagen (CTX), osteocalcin, PTH, 25(OH)D, anti-cardiolipin, anti-dsDNA, and anti-nucleosome levels were measured. PINP and CTX levels were elevated in SLE patients aged > 45 in comparison to those aged < 45, although with borderline significance (p = 0.05, respectively). Correlations were found between BTMs: the strongest being between PINP and osteocalcin (τ = 0.69, p < 0.05). PINP and osteocalcin were found to be associated with PTH (τ = 0.3, τ = 0.29, respectively, p < 0.05). Age correlated with PINP (τ = 0.23, p…
1Zakład Biochemii, Radioimmunologii i Medycyny Doświadczalnej, Instytut „Pomnik – Centrum Zdrowia... more 1Zakład Biochemii, Radioimmunologii i Medycyny Doświadczalnej, Instytut „Pomnik – Centrum Zdrowia Dziecka”, Warszawa Kierownik Zakładu: prof. dr hab. med. Roman Janas 2Klinika Endokrynologii, Centrum Medyczne Kształcenia Podyplomowego, Warszawa Kierownik Kliniki: prof. dr hab. med. Wojciech Zgliczyński 3Klinika Pediatrii, Reumatologii, Immunologii i Chorób Metabolicznych Kości, Uniwersytecki Dziecięcy Szpital Kliniczny im. Ludwika Zamenhofa w Białymstoku Kierownik Kliniki: dr hab. med. Jerzy Konstatynowicz 4Zakład Biochemii i Chemii Klinicznej, Warszawski Uniwersytet Medyczny Kierownik Zakładu: prof. dr hab. med. Jacek Łukaszkiewicz 5Klinika Geriatrii, Chorób Wewnętrznych i Chorób Metabolicznych Kości, Centrum Medyczne Kształcenia Podyplomowego, Warszawa Kierownik Kliniki: prof. dr hab. med. Marek Tałałaj
Biochemical and Biophysical Research Communications, 1978
ABSTRACT Anaerobic synthesis of catalase T (a typical oxygen-inducible haem containing enzyme) in... more ABSTRACT Anaerobic synthesis of catalase T (a typical oxygen-inducible haem containing enzyme) in the cz1 mutant of yeast was demonstrated. The synthesis of catalase T in anaerobically grown mutant cells is stimulated by haemin under carbon-derepressed conditions of growth (galactose as carbon source) but not in glucose repressed cultures. Haem is practically undetectable in the anaerobically grown glucose repressed wild type strain and its level in derepressed cells amounts to 3% of the fully derepressed aerobically grown cells. In the cz1 mutant cultures grown in anoxia both on galactose and glucose the haem level usually exceeds 10% of that in the aerobic control.
The aim of the study is to evaluate the advisability of systematic monitoring of clozapine (CLO) ... more The aim of the study is to evaluate the advisability of systematic monitoring of clozapine (CLO) concentration in serum during treatment of schizophrenia in Polish psychiatric patients. The concentration of CLO and its metabolites: norclozapine (NCLO) and clozapine N-oxide (CLO-NO) in serum obtained from 107 patients suffering from schizophrenia was determined by high performance liquid chromatography (HPLC) method. There were two groups of patients. In the first group of patients (n=95) the concentration of drug and its metabolites was determined by one-time testing. Correlations were tested using the test statistics. In the second group of patients (n=12), 51 samples of serum were provided by the same patient in different time spans (from 6days to 14 months after the beginning of the treatment). Concentrations of CLO and its metabolites in blood serum do not always show a linear dependence on the applied dose for individual patients. The high volatility of CLO concentrations in blood serum of patients treated with identical doses of the drug confirmed the validity of the monitored therapy.
Six children (five after partial intestinal resection, one after colectomy) were studied with res... more Six children (five after partial intestinal resection, one after colectomy) were studied with respect to absorption and metabolism of vitamin D. The results showed decreased 25-OH-D3 serum concentrations below 20 ng/ml (in two patients even below 10 ng/ml), possibly due to disturbed enterohepatic circulation of vitamin D metabolites.
Catalase A and T activities were investigated in two standard strains and three catalase regulato... more Catalase A and T activities were investigated in two standard strains and three catalase regulatory cgr mutants of yeast in respiratory competent and incompetent states, which were under various degrees of glucose repression. The formation of catalase A was very sensitive to glucose repression and was characterized by a long delay in derepression. Deprivation of the energy source in respiratory incompetent cells prevented the derepression of catalase A. The lack of catalase A in respiratory imcompetent cells can be overcome by growing the cells in raffinose or by the prolongation of the fermentative phase of derepression. Catalase T is under control of different regulatory systems probably common with some other haemoproteins.
To investigate the feasibility of bone turnover markers (BTMs) for the assessment of bone metabol... more To investigate the feasibility of bone turnover markers (BTMs) for the assessment of bone metabolism in patients with systemic lupus erythematosus (SLE), according to the guidelines of the International Osteoporosis Foundation and the International Federation of Clinical Chemistry and Laboratory Medicine. The study included 43 female SLE patients. Serum procollagen type I N propeptide (PINP), C-terminal telopeptide of type I collagen (CTX), osteocalcin, PTH, 25(OH)D, anti-cardiolipin, anti-dsDNA, and anti-nucleosome levels were measured. PINP and CTX levels were elevated in SLE patients aged > 45 in comparison to those aged < 45, although with borderline significance (p = 0.05, respectively). Correlations were found between BTMs: the strongest being between PINP and osteocalcin (τ = 0.69, p < 0.05). PINP and osteocalcin were found to be associated with PTH (τ = 0.3, τ = 0.29, respectively, p < 0.05). Age correlated with PINP (τ = 0.23, p…
1Zakład Biochemii, Radioimmunologii i Medycyny Doświadczalnej, Instytut „Pomnik – Centrum Zdrowia... more 1Zakład Biochemii, Radioimmunologii i Medycyny Doświadczalnej, Instytut „Pomnik – Centrum Zdrowia Dziecka”, Warszawa Kierownik Zakładu: prof. dr hab. med. Roman Janas 2Klinika Endokrynologii, Centrum Medyczne Kształcenia Podyplomowego, Warszawa Kierownik Kliniki: prof. dr hab. med. Wojciech Zgliczyński 3Klinika Pediatrii, Reumatologii, Immunologii i Chorób Metabolicznych Kości, Uniwersytecki Dziecięcy Szpital Kliniczny im. Ludwika Zamenhofa w Białymstoku Kierownik Kliniki: dr hab. med. Jerzy Konstatynowicz 4Zakład Biochemii i Chemii Klinicznej, Warszawski Uniwersytet Medyczny Kierownik Zakładu: prof. dr hab. med. Jacek Łukaszkiewicz 5Klinika Geriatrii, Chorób Wewnętrznych i Chorób Metabolicznych Kości, Centrum Medyczne Kształcenia Podyplomowego, Warszawa Kierownik Kliniki: prof. dr hab. med. Marek Tałałaj
Biochemical and Biophysical Research Communications, 1978
ABSTRACT Anaerobic synthesis of catalase T (a typical oxygen-inducible haem containing enzyme) in... more ABSTRACT Anaerobic synthesis of catalase T (a typical oxygen-inducible haem containing enzyme) in the cz1 mutant of yeast was demonstrated. The synthesis of catalase T in anaerobically grown mutant cells is stimulated by haemin under carbon-derepressed conditions of growth (galactose as carbon source) but not in glucose repressed cultures. Haem is practically undetectable in the anaerobically grown glucose repressed wild type strain and its level in derepressed cells amounts to 3% of the fully derepressed aerobically grown cells. In the cz1 mutant cultures grown in anoxia both on galactose and glucose the haem level usually exceeds 10% of that in the aerobic control.
The aim of the study is to evaluate the advisability of systematic monitoring of clozapine (CLO) ... more The aim of the study is to evaluate the advisability of systematic monitoring of clozapine (CLO) concentration in serum during treatment of schizophrenia in Polish psychiatric patients. The concentration of CLO and its metabolites: norclozapine (NCLO) and clozapine N-oxide (CLO-NO) in serum obtained from 107 patients suffering from schizophrenia was determined by high performance liquid chromatography (HPLC) method. There were two groups of patients. In the first group of patients (n=95) the concentration of drug and its metabolites was determined by one-time testing. Correlations were tested using the test statistics. In the second group of patients (n=12), 51 samples of serum were provided by the same patient in different time spans (from 6days to 14 months after the beginning of the treatment). Concentrations of CLO and its metabolites in blood serum do not always show a linear dependence on the applied dose for individual patients. The high volatility of CLO concentrations in blood serum of patients treated with identical doses of the drug confirmed the validity of the monitored therapy.
Six children (five after partial intestinal resection, one after colectomy) were studied with res... more Six children (five after partial intestinal resection, one after colectomy) were studied with respect to absorption and metabolism of vitamin D. The results showed decreased 25-OH-D3 serum concentrations below 20 ng/ml (in two patients even below 10 ng/ml), possibly due to disturbed enterohepatic circulation of vitamin D metabolites.
Catalase A and T activities were investigated in two standard strains and three catalase regulato... more Catalase A and T activities were investigated in two standard strains and three catalase regulatory cgr mutants of yeast in respiratory competent and incompetent states, which were under various degrees of glucose repression. The formation of catalase A was very sensitive to glucose repression and was characterized by a long delay in derepression. Deprivation of the energy source in respiratory incompetent cells prevented the derepression of catalase A. The lack of catalase A in respiratory imcompetent cells can be overcome by growing the cells in raffinose or by the prolongation of the fermentative phase of derepression. Catalase T is under control of different regulatory systems probably common with some other haemoproteins.
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