Una molecula de polinucleotido aislada que comprende un genoma o antigenoma del virus paragripal ... more Una molecula de polinucleotido aislada que comprende un genoma o antigenoma del virus paragripal (PIV) quimerico, que incluye un genoma o antigenoma de fondo de PIV parcial o completo de un PIV humano combinado con uno o mas genes o segmentos genomicos heterologos de uno o mas PIV diferentes, para formar un genoma o antigenoma de PIV quimerico humano-bovino, en el que al menos uno de los genes o segmentos genomicos heterologos codifica una proteina mayor de la nucleocapsida (N) de PIV bovino.
... Do you not see how necessary a World of Pains and troubles is to school an Intelligence and m... more ... Do you not see how necessary a World of Pains and troubles is to school an Intelligence and make it a soul? -John Keats iv Page 10. TABLE OF CONTENTS Abstract ..... ...
The photosynthetic characteristics of the green alga Chlamydomonas reinhardtii as well as other a... more The photosynthetic characteristics of the green alga Chlamydomonas reinhardtii as well as other algae and cyanobacteria are dependent upon the inorganic carbon concentration experienced by the alga during growth (1). Cells grown at air levels of CO2 (0,03%) have a much higher affinity for inorganic carbon (DIC) than cells grown at high levels of CO2 (>1%). Since the CO2 concentration during growth of the alga has no effect on either the Km(CO2) of the principal CO2 fixing enzyme, RuBP carboxylase/ oxygenase (Rubisco) or the mechanism of photosynthetic CO2 fixation, low DIC adapted algae must employ another method for increasing the efficiency of CO2 utilization (2). It is now apparent that the high affinity for DIC exhibited by these algae can be traced to a system that effectively saturates Rubisco with CO2 and eliminates O2 inhibition of photosynthesis. This system is dependent upon two separate components: carbonic anhydrase (CA), an enzyme primarily localized in the periplasmic space and responsible for maintaining HCO3/CO2 equilibrium; and an inorganic carbon concentrating mechanism that actively transports inorganic carbon into the cell (2), Mutants of Chlamydomonas that are deficient in CA activity or DIC transport exhibit low affinity for inorganic carbon (3,4). Growth at high levels of DIC inhibits the expression of CA activity and DIC transport (5).
The Kansas/15626/84 (Ka) and Shipping Fever (SF) strains of bovine parainfluenza virus type 3 (BP... more The Kansas/15626/84 (Ka) and Shipping Fever (SF) strains of bovine parainfluenza virus type 3 (BPIV3) replicate less efficiently than human PIV3 (HPIV3) in the upper and lower respiratory tract of rhesus monkeys, and BPIV3 Ka is also highly attenuated in humans and is in clinical trials as a candidate vaccine against HPIV3. To initiate an investigation of the genetic basis
This study examines the contribution of the fusion (F) and hemagglutinin-neuraminidase (HN) glyco... more This study examines the contribution of the fusion (F) and hemagglutinin-neuraminidase (HN) glycoprotein genes of bovine parainfluenza virus type 3 (BPIV3) to its restricted replication in the respiratory tract of nonhuman primates. A chimeric recombinant human parainfluenza type 3 virus (HPIV3) containing BPIV3 F and HN glycoprotein genes in place of its own and the reciprocal recombinant consisting of BPIV3 bearing the HPIV3 F and HN genes (rBPIV3-F H HN H ) were generated to assess the effect of glycoprotein substitution on replication of HPIV3 and BPIV3 in the upper and lower respiratory tract of rhesus monkeys. The chimeric viruses were readily recovered and replicated in simian LLC-MK2 cells to a level comparable to that of their parental viruses, suggesting that the heterologous glycoproteins were compatible with the PIV3 internal proteins. HPIV3 bearing the BPIV3 F and HN genes was restricted in replication in rhesus monkeys to a level similar to that of its BPIV3 parent vir...
The shipping fever (SF) and Kansas (Ka) strains of bovine parainfluenza virus type 3 (BPIV3) are ... more The shipping fever (SF) and Kansas (Ka) strains of bovine parainfluenza virus type 3 (BPIV3) are restricted in their replication in rhesus monkeys 100- to 1,000-fold compared to human parainfluenza virus type 3 (HPIV3), and the Ka strain also was shown to be attenuated in humans. To initiate an investigation of the genetic basis of the attenuation of BPIV3 in primates, we produced viable chimeric HPIV3 recombinants containing the nucleoprotein (N) open reading frame (ORF) from either BPIV3 Ka or SF in place of the HPIV3 N ORF. These chimeric recombinants were designated cKa-N and cSF-N, respectively. Remarkably, cKa-N and cSF-N grew to titers comparable to those of their HPIV3 and BPIV3 parents in LLC-MK2 monkey kidney and Madin-Darby bovine kidney cells. Thus, the heterologous nature of the N protein did not impede replication in vitro. However, cKa-N and cSF-N were each restricted in replication in rhesus monkeys to a similar extent as Ka and SF, respectively. This identified the ...
Journal of the American Society of Nephrology, 2003
ABSTRACT. Delayed graft function (DGF) is the need for dialysis in the first week after transplan... more ABSTRACT. Delayed graft function (DGF) is the need for dialysis in the first week after transplantation. Studied were risk factors for DGF in adult (age ≥16 yr) cadaveric renal transplant recipients by means of a multivariable modeling procedure. Only donor and recipient factors known before transplantation were chosen so that the probabilities of DGF could be calculated before transplantation and appropriate preventative measures taken. Data on 19,706 recipients of cadaveric allografts were obtained from the United States Renal Data System registry (1995 to 1998). Graft losses within the first 24 h after surgery were excluded from the analysis (n = 89). Patients whose DGF information was missing or unknown (n = 2820) and patients missing one or more candidate predictors (n = 2951) were also excluded. By means of a multivariable logistic regression analysis, factors contributing to DGF in the remaining 13,846 patients were identified. After validating the logistic regression model, ...
Recent studies have shown the gene encoding creatine kinase isoform M (CKMM) to be very closely l... more Recent studies have shown the gene encoding creatine kinase isoform M (CKMM) to be very closely linked to the myotonic dystrophy (DM) locus on the long arm of chromosome 19. Given this close linkage to DM and the postulated role of CKMM in skeletal muscle contraction, the possibility of a defect in CKMM causing DM was investigated. CKMM cDNA was isolated from the skeletal muscle of an individual with DM. Sequencing of the CKMM cDNA from the DM chromosome 19 revealed two novel polymorphisms but no translationally significant mutation. This work rules out a defect in the coding segment of CKMM as a cause of DM in this family and, in light of genetic homogeneity shown to date for DM, probably in all cases of DM.
Una molecula de polinucleotido aislada que comprende un genoma o antigenoma del virus paragripal ... more Una molecula de polinucleotido aislada que comprende un genoma o antigenoma del virus paragripal (PIV) quimerico, que incluye un genoma o antigenoma de fondo de PIV parcial o completo de un PIV humano combinado con uno o mas genes o segmentos genomicos heterologos de uno o mas PIV diferentes, para formar un genoma o antigenoma de PIV quimerico humano-bovino, en el que al menos uno de los genes o segmentos genomicos heterologos codifica una proteina mayor de la nucleocapsida (N) de PIV bovino.
... Do you not see how necessary a World of Pains and troubles is to school an Intelligence and m... more ... Do you not see how necessary a World of Pains and troubles is to school an Intelligence and make it a soul? -John Keats iv Page 10. TABLE OF CONTENTS Abstract ..... ...
The photosynthetic characteristics of the green alga Chlamydomonas reinhardtii as well as other a... more The photosynthetic characteristics of the green alga Chlamydomonas reinhardtii as well as other algae and cyanobacteria are dependent upon the inorganic carbon concentration experienced by the alga during growth (1). Cells grown at air levels of CO2 (0,03%) have a much higher affinity for inorganic carbon (DIC) than cells grown at high levels of CO2 (>1%). Since the CO2 concentration during growth of the alga has no effect on either the Km(CO2) of the principal CO2 fixing enzyme, RuBP carboxylase/ oxygenase (Rubisco) or the mechanism of photosynthetic CO2 fixation, low DIC adapted algae must employ another method for increasing the efficiency of CO2 utilization (2). It is now apparent that the high affinity for DIC exhibited by these algae can be traced to a system that effectively saturates Rubisco with CO2 and eliminates O2 inhibition of photosynthesis. This system is dependent upon two separate components: carbonic anhydrase (CA), an enzyme primarily localized in the periplasmic space and responsible for maintaining HCO3/CO2 equilibrium; and an inorganic carbon concentrating mechanism that actively transports inorganic carbon into the cell (2), Mutants of Chlamydomonas that are deficient in CA activity or DIC transport exhibit low affinity for inorganic carbon (3,4). Growth at high levels of DIC inhibits the expression of CA activity and DIC transport (5).
The Kansas/15626/84 (Ka) and Shipping Fever (SF) strains of bovine parainfluenza virus type 3 (BP... more The Kansas/15626/84 (Ka) and Shipping Fever (SF) strains of bovine parainfluenza virus type 3 (BPIV3) replicate less efficiently than human PIV3 (HPIV3) in the upper and lower respiratory tract of rhesus monkeys, and BPIV3 Ka is also highly attenuated in humans and is in clinical trials as a candidate vaccine against HPIV3. To initiate an investigation of the genetic basis
This study examines the contribution of the fusion (F) and hemagglutinin-neuraminidase (HN) glyco... more This study examines the contribution of the fusion (F) and hemagglutinin-neuraminidase (HN) glycoprotein genes of bovine parainfluenza virus type 3 (BPIV3) to its restricted replication in the respiratory tract of nonhuman primates. A chimeric recombinant human parainfluenza type 3 virus (HPIV3) containing BPIV3 F and HN glycoprotein genes in place of its own and the reciprocal recombinant consisting of BPIV3 bearing the HPIV3 F and HN genes (rBPIV3-F H HN H ) were generated to assess the effect of glycoprotein substitution on replication of HPIV3 and BPIV3 in the upper and lower respiratory tract of rhesus monkeys. The chimeric viruses were readily recovered and replicated in simian LLC-MK2 cells to a level comparable to that of their parental viruses, suggesting that the heterologous glycoproteins were compatible with the PIV3 internal proteins. HPIV3 bearing the BPIV3 F and HN genes was restricted in replication in rhesus monkeys to a level similar to that of its BPIV3 parent vir...
The shipping fever (SF) and Kansas (Ka) strains of bovine parainfluenza virus type 3 (BPIV3) are ... more The shipping fever (SF) and Kansas (Ka) strains of bovine parainfluenza virus type 3 (BPIV3) are restricted in their replication in rhesus monkeys 100- to 1,000-fold compared to human parainfluenza virus type 3 (HPIV3), and the Ka strain also was shown to be attenuated in humans. To initiate an investigation of the genetic basis of the attenuation of BPIV3 in primates, we produced viable chimeric HPIV3 recombinants containing the nucleoprotein (N) open reading frame (ORF) from either BPIV3 Ka or SF in place of the HPIV3 N ORF. These chimeric recombinants were designated cKa-N and cSF-N, respectively. Remarkably, cKa-N and cSF-N grew to titers comparable to those of their HPIV3 and BPIV3 parents in LLC-MK2 monkey kidney and Madin-Darby bovine kidney cells. Thus, the heterologous nature of the N protein did not impede replication in vitro. However, cKa-N and cSF-N were each restricted in replication in rhesus monkeys to a similar extent as Ka and SF, respectively. This identified the ...
Journal of the American Society of Nephrology, 2003
ABSTRACT. Delayed graft function (DGF) is the need for dialysis in the first week after transplan... more ABSTRACT. Delayed graft function (DGF) is the need for dialysis in the first week after transplantation. Studied were risk factors for DGF in adult (age ≥16 yr) cadaveric renal transplant recipients by means of a multivariable modeling procedure. Only donor and recipient factors known before transplantation were chosen so that the probabilities of DGF could be calculated before transplantation and appropriate preventative measures taken. Data on 19,706 recipients of cadaveric allografts were obtained from the United States Renal Data System registry (1995 to 1998). Graft losses within the first 24 h after surgery were excluded from the analysis (n = 89). Patients whose DGF information was missing or unknown (n = 2820) and patients missing one or more candidate predictors (n = 2951) were also excluded. By means of a multivariable logistic regression analysis, factors contributing to DGF in the remaining 13,846 patients were identified. After validating the logistic regression model, ...
Recent studies have shown the gene encoding creatine kinase isoform M (CKMM) to be very closely l... more Recent studies have shown the gene encoding creatine kinase isoform M (CKMM) to be very closely linked to the myotonic dystrophy (DM) locus on the long arm of chromosome 19. Given this close linkage to DM and the postulated role of CKMM in skeletal muscle contraction, the possibility of a defect in CKMM causing DM was investigated. CKMM cDNA was isolated from the skeletal muscle of an individual with DM. Sequencing of the CKMM cDNA from the DM chromosome 19 revealed two novel polymorphisms but no translationally significant mutation. This work rules out a defect in the coding segment of CKMM as a cause of DM in this family and, in light of genetic homogeneity shown to date for DM, probably in all cases of DM.
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Papers by Jane Bailly