Herpes simplex virus 1 (HSV-1) ICP22 is a multifunctional protein and important for HSV-1 replica... more Herpes simplex virus 1 (HSV-1) ICP22 is a multifunctional protein and important for HSV-1 replication. Pseudorabies virus (PRV) ICP22 (P-ICP22) is a homologue of HSV-1 ICP22 and is reported to be able to selectively modify the transcription of different kinetic classes of PRV genes, however, the subcellular localization, localization signal and molecular determinants for its transport to execute this function is less well understood. In this study, by utilizing live cells fluorescent microscopy, P-ICP22 fused to enhanced yellow fluorescent protein (EYFP) gene was transient expressed in live cells and shown to exhibit a predominantly nucleus localization in the absence of other viral proteins. By transfection of a series of P-ICP22 deletion mutants fused to EYFP, a bona fide nuclear localization signal (NLS) and its key amino acids (aa) of P-ICP22 was, for the first time, determined and mapped to aa 41-60 (PASTPTPPKRGRYVVEHPEY) and aa 49-50 (KR), respectively. Besides, the P-ICP22 wa...
2008 International Workshop on Education Technology and Training & 2008 International Workshop on Geoscience and Remote Sensing, 2008
... Wang Jinlin1,2, Chen Xi1, Zhou Kefa1, Wang Wei1,Zhang Dan3 1 Xinjiang Institute of Geography ... more ... Wang Jinlin1,2, Chen Xi1, Zhou Kefa1, Wang Wei1,Zhang Dan3 1 Xinjiang Institute of Geography and Ecology Chinese Academy of ... Users can control each step of data mining and knowledge discovery and carry out man-machine interaction repeatedly to obtain satisfactory ...
2009 Third International Symposium on Intelligent Information Technology Application, 2009
... We performed the experiments with two machines whose configurations were as follows. Testing ... more ... We performed the experiments with two machines whose configurations were as follows. Testing machine 1: Intel(R) Pentium(R) 4 CPU 2.40GHz, 1GB memory, Microsoft Windows XP, Installed cygwin emulating Linux environment. ...
Archives of biochemistry and biophysics, Jan 5, 2015
Herpes simplex virus 1 (HSV-1) UL31 is a multifunctional protein and important for HSV-1 infectio... more Herpes simplex virus 1 (HSV-1) UL31 is a multifunctional protein and important for HSV-1 infection. Pseudorabies virus (PRV) UL31 is a late protein homologous to HSV-1 UL31. Previous studies showed that PRV UL31 is predominantly localized to nucleus, however, the molecular determinants for its nuclear import were unclear to date. Here, by utilizing live cells fluorescent microscopy, UL31 fused with enhanced yellow fluorescent protein was transiently expressed in live cells and confirmed to exlusively target to the nucleus in the absence of other viral proteins. Furthermore, the nuclear import of UL31 was found to be dependent on the Ran-, importin α1-, α3-, α5-, α7-, β1-and transportin-1-mediated pathway. Therefore, these results would open up new avenues for depicting the biological functions of UL31 during PRV infection.
The pseudorabies virus (PRV) UL31 protein (pUL31) is a homologue of the herpes simplex virus 1 pU... more The pseudorabies virus (PRV) UL31 protein (pUL31) is a homologue of the herpes simplex virus 1 pUL31, which is a multifunctional protein that is important for HSV-1 infection. However, little is known concerning the subcellular localization signal of PRV UL31. Here, by transfection with a series of PRV UL31 deletion mutants fused to an enhanced yellow fluorescent protein (EYFP) gene, a bipartite nuclear localization signal (NLS) and a PY motif NLS of UL31 were identified and mapped to amino acids (aa) 4 to 20 (RRRLLRRKSSAARRKTL) and aa 21 to 34 (TRAARDRYAPYFAY), respectively. Additionally, the predicted nuclear export signal (NES) was shown to be nonfunctional. Taken together, this information opens up new avenues for investigating the biological functions of UL31 during PRV infection.
International journal of clinical and experimental pathology, 2015
IgG4-related lung disease (IgG4-RLD) is recently emerging entity. Several reports concerned with ... more IgG4-related lung disease (IgG4-RLD) is recently emerging entity. Several reports concerned with the clinicopathologic feature have been described, but this disease in puerperium has not been reported previously. Here, we report a 24-year-old woman diagnosed as IgG4-RLD in puerperium, who developed dry cough, low fever and exertional dyspnea following the delivery. The inflammatory markers and pulmonary lesions of the patient suggested pneumonia. However, there was no improvement after antibiotic treatment. The infiltration of IgG4-positive lymphoplasmacytes was found in lung biopsy by video-assisted thoracic surgery (VATS). And the serum IgG4 level was high. The patient was effectively treated with corticosteroids. This unique case highlights the occurrence of IgG4-RLD in puerperium and underscores it should be taken into consideration as a possible differential diagnosis when dense lymphoplasmacytic infiltration was found in pulmonary consolidation in complex puerperal respiratory...
2009 WASE International Conference on Information Engineering, 2009
Most current reputation models fail to calculate peerspsila reputation accurately when there is l... more Most current reputation models fail to calculate peerspsila reputation accurately when there is little transaction history between the peers. To this end, this paper presents a trust model combing reputation and credential which accepts inputs of both feedback and credential. The model is based on reputation-based trust model, and according to the correlation of credential and reputation, the model can
2008 International Conference on Computer Science and Software Engineering, 2008
The replication and deployment of media file is one of the key issues in video-on-demand system, ... more The replication and deployment of media file is one of the key issues in video-on-demand system, the replication algorithm and deployment strategy will directly affect the performance of storage balance and load balance in the system. In order to enhance the capacity of streaming media servers and handle the problem that migrating files will affect acceptance rate of user requests,
2006 8th international Conference on Signal Processing, 2006
It is of great importance to improve the performance of ring laser gyroscopes by ameliorating the... more It is of great importance to improve the performance of ring laser gyroscopes by ameliorating the lock characteristic. After careful research on lock control principle, lock minimization and lock stabilization of back-scattering are presented. Frequency stabilization and lock stabilization control system is designed, and is demonstrated by a ring laser gyroscope frequency stability and lock stabilization parameter test system which is developed on the basis of the technique. The result shows that the technique fully satisfies design requirements of the ring laser gyroscope
To retrospectively investigate the role of (18) F-fluorodeoxyglucose positron emission tomography... more To retrospectively investigate the role of (18) F-fluorodeoxyglucose positron emission tomography/computed tomography ((18)F-FDG PET/CT) for the diagnosis and therapeutic response in relapsing polychondritis (RP) patients. (18)F-FDG PET/CT findings were reviewed in six RP patients. The initial scans were performed for all patients, follow-up scans were performed during steroid therapy for five patients. Changes in the abnormal lesions and the maximal standard uptake value (SUVmax) were analyzed. The initial PET/CT scans revealed intense FDG uptake in the cartilages for all six patients. The lesions of abnormal FDG uptake were tracheal/bronchial cartilage (n = 4), costicartilage (n = 4), nasal cartilage (n = 3), cricoid cartilage (n = 3), auricular cartilage (n = 3), arytenoid cartilage (n = 3), thyroid cartilage (n = 2), hyoid cartilage (n = 1) and mediastinum lymph node (n = 1). The mean visual score and the mean SUVmax were 2.96 ± 0.20 and 4.10 ± 0.6. The intense uptake reduced or...
53rd Electronic Components and Technology Conference, 2003. Proceedings., 2003
Abstract A brittle solder joint at the interface between a solder ball and a substrate metal pad ... more Abstract A brittle solder joint at the interface between a solder ball and a substrate metal pad could result in an open joint failure after the solder ball is attached to a mothehoard. Esisting metrologies_ such as ball shear. do not always detect the weak solder joint strength. A ...
A brittle solder joint at the interface between a solder ball and a substrate metal pad could res... more A brittle solder joint at the interface between a solder ball and a substrate metal pad could result in an open joint failure after the solder ball is attached to a motherboard. Existing metrologies, such as ball shear, do not always detect the weak solder joint strength. A ...
ABSTRACT The concept of approximate inertial manifold (AIM) is extended to develop a kind of nonl... more ABSTRACT The concept of approximate inertial manifold (AIM) is extended to develop a kind of nonlinear order reduction technique for non-autonomous nonlinear systems in second-order form in this paper. Using the modal transformation, a large nonlinear dynamical system is split into a ‘master’ subsystem, a ‘slave’ subsystem, and a ‘negligible’ subsystem. Accordingly, a novel order reduction method (Method I) is developed to construct a low order subsystem by neglecting the ‘negligible’ subsystem and slaving the ‘slave’ subsystem into the ‘master’ subsystem using the extended AIM. As a comparison, Method II accounting for the effects of both ‘slave’ subsystem and the ‘negligible’ subsystem is also applied to obtain the reduced order subsystem. Then, a typical 5-degree-of-freedom nonlinear dynamical system is given to compare the accuracy and efficiency of the traditional Galerkin truncation (ignoring the contributions of the slave and negligible subsystems), Method I and Method II. It is shown that Method I gives a considerable increase in accuracy for little computational cost in comparison with the standard Galerkin method, and produces almost the same accuracy as Method II. Finally, a 3-degree-of-freedom nonlinear dynamical system is analyzed by using the analytic method for showing predominance and convenience of Method I to obtain the analytically reduced order system. KeywordsGalerkin method-nonlinear systems-dimension reduction-post-processed method-model truncation
Herpes simplex virus 1 (HSV-1) ICP22 is a multifunctional protein and important for HSV-1 replica... more Herpes simplex virus 1 (HSV-1) ICP22 is a multifunctional protein and important for HSV-1 replication. Pseudorabies virus (PRV) ICP22 (P-ICP22) is a homologue of HSV-1 ICP22 and is reported to be able to selectively modify the transcription of different kinetic classes of PRV genes, however, the subcellular localization, localization signal and molecular determinants for its transport to execute this function is less well understood. In this study, by utilizing live cells fluorescent microscopy, P-ICP22 fused to enhanced yellow fluorescent protein (EYFP) gene was transient expressed in live cells and shown to exhibit a predominantly nucleus localization in the absence of other viral proteins. By transfection of a series of P-ICP22 deletion mutants fused to EYFP, a bona fide nuclear localization signal (NLS) and its key amino acids (aa) of P-ICP22 was, for the first time, determined and mapped to aa 41-60 (PASTPTPPKRGRYVVEHPEY) and aa 49-50 (KR), respectively. Besides, the P-ICP22 wa...
2008 International Workshop on Education Technology and Training & 2008 International Workshop on Geoscience and Remote Sensing, 2008
... Wang Jinlin1,2, Chen Xi1, Zhou Kefa1, Wang Wei1,Zhang Dan3 1 Xinjiang Institute of Geography ... more ... Wang Jinlin1,2, Chen Xi1, Zhou Kefa1, Wang Wei1,Zhang Dan3 1 Xinjiang Institute of Geography and Ecology Chinese Academy of ... Users can control each step of data mining and knowledge discovery and carry out man-machine interaction repeatedly to obtain satisfactory ...
2009 Third International Symposium on Intelligent Information Technology Application, 2009
... We performed the experiments with two machines whose configurations were as follows. Testing ... more ... We performed the experiments with two machines whose configurations were as follows. Testing machine 1: Intel(R) Pentium(R) 4 CPU 2.40GHz, 1GB memory, Microsoft Windows XP, Installed cygwin emulating Linux environment. ...
Archives of biochemistry and biophysics, Jan 5, 2015
Herpes simplex virus 1 (HSV-1) UL31 is a multifunctional protein and important for HSV-1 infectio... more Herpes simplex virus 1 (HSV-1) UL31 is a multifunctional protein and important for HSV-1 infection. Pseudorabies virus (PRV) UL31 is a late protein homologous to HSV-1 UL31. Previous studies showed that PRV UL31 is predominantly localized to nucleus, however, the molecular determinants for its nuclear import were unclear to date. Here, by utilizing live cells fluorescent microscopy, UL31 fused with enhanced yellow fluorescent protein was transiently expressed in live cells and confirmed to exlusively target to the nucleus in the absence of other viral proteins. Furthermore, the nuclear import of UL31 was found to be dependent on the Ran-, importin α1-, α3-, α5-, α7-, β1-and transportin-1-mediated pathway. Therefore, these results would open up new avenues for depicting the biological functions of UL31 during PRV infection.
The pseudorabies virus (PRV) UL31 protein (pUL31) is a homologue of the herpes simplex virus 1 pU... more The pseudorabies virus (PRV) UL31 protein (pUL31) is a homologue of the herpes simplex virus 1 pUL31, which is a multifunctional protein that is important for HSV-1 infection. However, little is known concerning the subcellular localization signal of PRV UL31. Here, by transfection with a series of PRV UL31 deletion mutants fused to an enhanced yellow fluorescent protein (EYFP) gene, a bipartite nuclear localization signal (NLS) and a PY motif NLS of UL31 were identified and mapped to amino acids (aa) 4 to 20 (RRRLLRRKSSAARRKTL) and aa 21 to 34 (TRAARDRYAPYFAY), respectively. Additionally, the predicted nuclear export signal (NES) was shown to be nonfunctional. Taken together, this information opens up new avenues for investigating the biological functions of UL31 during PRV infection.
International journal of clinical and experimental pathology, 2015
IgG4-related lung disease (IgG4-RLD) is recently emerging entity. Several reports concerned with ... more IgG4-related lung disease (IgG4-RLD) is recently emerging entity. Several reports concerned with the clinicopathologic feature have been described, but this disease in puerperium has not been reported previously. Here, we report a 24-year-old woman diagnosed as IgG4-RLD in puerperium, who developed dry cough, low fever and exertional dyspnea following the delivery. The inflammatory markers and pulmonary lesions of the patient suggested pneumonia. However, there was no improvement after antibiotic treatment. The infiltration of IgG4-positive lymphoplasmacytes was found in lung biopsy by video-assisted thoracic surgery (VATS). And the serum IgG4 level was high. The patient was effectively treated with corticosteroids. This unique case highlights the occurrence of IgG4-RLD in puerperium and underscores it should be taken into consideration as a possible differential diagnosis when dense lymphoplasmacytic infiltration was found in pulmonary consolidation in complex puerperal respiratory...
2009 WASE International Conference on Information Engineering, 2009
Most current reputation models fail to calculate peerspsila reputation accurately when there is l... more Most current reputation models fail to calculate peerspsila reputation accurately when there is little transaction history between the peers. To this end, this paper presents a trust model combing reputation and credential which accepts inputs of both feedback and credential. The model is based on reputation-based trust model, and according to the correlation of credential and reputation, the model can
2008 International Conference on Computer Science and Software Engineering, 2008
The replication and deployment of media file is one of the key issues in video-on-demand system, ... more The replication and deployment of media file is one of the key issues in video-on-demand system, the replication algorithm and deployment strategy will directly affect the performance of storage balance and load balance in the system. In order to enhance the capacity of streaming media servers and handle the problem that migrating files will affect acceptance rate of user requests,
2006 8th international Conference on Signal Processing, 2006
It is of great importance to improve the performance of ring laser gyroscopes by ameliorating the... more It is of great importance to improve the performance of ring laser gyroscopes by ameliorating the lock characteristic. After careful research on lock control principle, lock minimization and lock stabilization of back-scattering are presented. Frequency stabilization and lock stabilization control system is designed, and is demonstrated by a ring laser gyroscope frequency stability and lock stabilization parameter test system which is developed on the basis of the technique. The result shows that the technique fully satisfies design requirements of the ring laser gyroscope
To retrospectively investigate the role of (18) F-fluorodeoxyglucose positron emission tomography... more To retrospectively investigate the role of (18) F-fluorodeoxyglucose positron emission tomography/computed tomography ((18)F-FDG PET/CT) for the diagnosis and therapeutic response in relapsing polychondritis (RP) patients. (18)F-FDG PET/CT findings were reviewed in six RP patients. The initial scans were performed for all patients, follow-up scans were performed during steroid therapy for five patients. Changes in the abnormal lesions and the maximal standard uptake value (SUVmax) were analyzed. The initial PET/CT scans revealed intense FDG uptake in the cartilages for all six patients. The lesions of abnormal FDG uptake were tracheal/bronchial cartilage (n = 4), costicartilage (n = 4), nasal cartilage (n = 3), cricoid cartilage (n = 3), auricular cartilage (n = 3), arytenoid cartilage (n = 3), thyroid cartilage (n = 2), hyoid cartilage (n = 1) and mediastinum lymph node (n = 1). The mean visual score and the mean SUVmax were 2.96 ± 0.20 and 4.10 ± 0.6. The intense uptake reduced or...
53rd Electronic Components and Technology Conference, 2003. Proceedings., 2003
Abstract A brittle solder joint at the interface between a solder ball and a substrate metal pad ... more Abstract A brittle solder joint at the interface between a solder ball and a substrate metal pad could result in an open joint failure after the solder ball is attached to a mothehoard. Esisting metrologies_ such as ball shear. do not always detect the weak solder joint strength. A ...
A brittle solder joint at the interface between a solder ball and a substrate metal pad could res... more A brittle solder joint at the interface between a solder ball and a substrate metal pad could result in an open joint failure after the solder ball is attached to a motherboard. Existing metrologies, such as ball shear, do not always detect the weak solder joint strength. A ...
ABSTRACT The concept of approximate inertial manifold (AIM) is extended to develop a kind of nonl... more ABSTRACT The concept of approximate inertial manifold (AIM) is extended to develop a kind of nonlinear order reduction technique for non-autonomous nonlinear systems in second-order form in this paper. Using the modal transformation, a large nonlinear dynamical system is split into a ‘master’ subsystem, a ‘slave’ subsystem, and a ‘negligible’ subsystem. Accordingly, a novel order reduction method (Method I) is developed to construct a low order subsystem by neglecting the ‘negligible’ subsystem and slaving the ‘slave’ subsystem into the ‘master’ subsystem using the extended AIM. As a comparison, Method II accounting for the effects of both ‘slave’ subsystem and the ‘negligible’ subsystem is also applied to obtain the reduced order subsystem. Then, a typical 5-degree-of-freedom nonlinear dynamical system is given to compare the accuracy and efficiency of the traditional Galerkin truncation (ignoring the contributions of the slave and negligible subsystems), Method I and Method II. It is shown that Method I gives a considerable increase in accuracy for little computational cost in comparison with the standard Galerkin method, and produces almost the same accuracy as Method II. Finally, a 3-degree-of-freedom nonlinear dynamical system is analyzed by using the analytic method for showing predominance and convenience of Method I to obtain the analytically reduced order system. KeywordsGalerkin method-nonlinear systems-dimension reduction-post-processed method-model truncation
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