Different genetic biomarkers have been used to evaluate the pollution effects of mutagenic agents... more Different genetic biomarkers have been used to evaluate the pollution effects of mutagenic agents such as metals and also a great variety of chemicals delivered on the environment by human activities. This way, the aim of the present report was to evaluate the effects of inorganic lead in fishes through the frequency of piscine micronuclei and nuclear morphological alterations in peripheral cells, chromosomal aberration frequency and comet assays in blood and kidney cells. Specimens of Hoplias malabaricus received different doses of lead by intra-peritoneal injections at time of 96 h. There was not a significant difference between control and treated groups for the piscine micronucleus and chromosomal aberration assays. In the comet assays there was a significant difference between control and contaminated groups. However, a significant difference between the applied doses was not observed. The results obtained with the comet assays also show that blood presented a higher sensibility than the kidney tissue, possibly due to the acute contamination. Although the results showed the genotoxic potential of lead at the 21 and 63 µg Pb2 + /g doses for both tissues, the lowest dose is considered more appropriate for future bioassays.
A fase exploratória do desenvolvimento de novos medicamentos implica a condução de ensaios de com... more A fase exploratória do desenvolvimento de novos medicamentos implica a condução de ensaios de complexidade crescente. Os estudos clínicos aleatorizados conduzidos num único doente (n= 1) poderão constituir uma alternativa para a avaliação do potencial ...
Page 1. Botulinum toxin type A formulations in focal dystonias (Protocol) Costa J, Ferreira J, Fr... more Page 1. Botulinum toxin type A formulations in focal dystonias (Protocol) Costa J, Ferreira J, Freire R, Coelho MM, Sampaio C This is a reprint of a Cochrane protocol, prepared and maintained by The Cochrane Collaboration ...
Different genetic biomarkers have been used to evaluate the pollution effects of mutagenic agents... more Different genetic biomarkers have been used to evaluate the pollution effects of mutagenic agents such as metals and also a great variety of chemicals delivered on the environment by human activities. This way, the aim of the present report was to evaluate the effects of inorganic lead in fishes through the frequency of piscine micronuclei and nuclear morphological alterations in peripheral cells, chromosomal aberration frequency and comet assays in blood and kidney cells. Specimens of Hoplias malabaricus received different doses of lead by intra-peritoneal injections at time of 96 h. There was not a significant difference between control and treated groups for the piscine micronucleus and chromosomal aberration assays. In the comet assays there was a significant difference between control and contaminated groups. However, a significant difference between the applied doses was not observed. The results obtained with the comet assays also show that blood presented a higher sensibility than the kidney tissue, possibly due to the acute contamination. Although the results showed the genotoxic potential of lead at the 21 and 63 µg Pb2 + /g doses for both tissues, the lowest dose is considered more appropriate for future bioassays.
A fase exploratória do desenvolvimento de novos medicamentos implica a condução de ensaios de com... more A fase exploratória do desenvolvimento de novos medicamentos implica a condução de ensaios de complexidade crescente. Os estudos clínicos aleatorizados conduzidos num único doente (n= 1) poderão constituir uma alternativa para a avaliação do potencial ...
Page 1. Botulinum toxin type A formulations in focal dystonias (Protocol) Costa J, Ferreira J, Fr... more Page 1. Botulinum toxin type A formulations in focal dystonias (Protocol) Costa J, Ferreira J, Freire R, Coelho MM, Sampaio C This is a reprint of a Cochrane protocol, prepared and maintained by The Cochrane Collaboration ...
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