As Nutrition and Toxicology Section Chief for the MSU Veterinary Diagnostic Laboratory I am responsible for the analytical tests performed, the results, and their interpretations. Analysis of unknown contaminants and intentional poisoning incidents in companion animal and wildlife populations are routine tests performed within my section. Additionally, my lab helps scientific professionals quantitate various organic drugs and pesticides and provide analytical support to pharmacokinetic/toxicokinetic studies.
Journal of the American Veterinary Medical Association, 2020
OBJECTIVE To compare the cause of death (COD; whether by natural death or euthanasia for poor qua... more OBJECTIVE To compare the cause of death (COD; whether by natural death or euthanasia for poor quality of life caused by a primary pathological condition) between search-and-rescue (SAR) dogs deployed to the World Trade Center, Pentagon, or Fresh Kills Landfill on Staten Island following the 9/11 terrorist attacks and SAR dogs that were not deployed to these sites. ANIMALS 95 deployed SAR dogs (exposed dogs) and 55 nondeployed SAR dogs (unexposed dogs). PROCEDURES Following natural death or euthanasia, 63 dogs (44 exposed and 19 unexposed) underwent a necropsy examination. For the remaining 87 dogs, the COD was categorized on the basis of information obtained from medical records or personal communications. RESULTS The median age of death was 12.8 years for exposed dogs and 12.7 years for unexposed dogs. The COD was not impacted by deployment status. In the 150 exposed and unexposed dogs, degenerative conditions were the most common COD followed by neoplasia. Respiratory disease was ...
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 2018
A 7-y-old Weimaraner bitch was presented to emergency service after 3 h of active labor with no p... more A 7-y-old Weimaraner bitch was presented to emergency service after 3 h of active labor with no puppies produced. Hemoabdomen and hemothorax were present at the time of surgery; prothrombin time (PT) and activated partial thromboplastin time (aPTT) were both found to be within normal ranges. Surgical cesarean section was performed; 4 dead puppies and 5 live puppies were delivered. Because hemostasis was difficult to achieve, a hysterectomy was performed; however, the dog died as the operation was being completed. At autopsy, the pleural cavity contained 1.5 L of unclotted blood; the peritoneal cavity was relatively normal, and no obvious hemorrhage was associated with the surgical sites. All 4 dead fetuses were opened, and their pleural cavities were filled with unclotted blood. An anticoagulant screen was performed, and brodifacoum was identified in the liver of the bitch. This case is unusual in that the PT and aPTT were within reference intervals, but brodifacoum was present in s...
We have previously reported that Δ-9-tetrahydrocannabinol (Δ9-THC)-treated mice challenged with i... more We have previously reported that Δ-9-tetrahydrocannabinol (Δ9-THC)-treated mice challenged with influenza virus A/PR/8/34 (PR8) developed increased viral hemagglutinin 1 (H1) mRNA levels and decreased monocyte and lymphocyte recruitment to the pulmonary airways when compared with mice challenged with PR8 alone. The objective of the present study was to examine the role of cannabinoid (CB1/CB2) receptors in mediating the effects of Δ9-THC on immune and epithelial cell responses to PR8. In the current study, Δ9-THC-treated CB1/CB2 receptor null (CB1−/−/CB2−/−) and wild-type mice infected with PR8 had marked increases in viral H1 mRNA when compared with CB1−/−/CB2−/− and wild-type mice challenged with PR8 alone. However, the magnitude of the H1 mRNA levels was greatly reduced in CB1−/−/CB2−/− mice as compared with wild-type mice. In addition, Δ9-THC-treated CB1−/−/CB2−/− mice infected with PR8 had increased CD4+ T cells and IFN-γ in bronchoalveolar lavage fluid with greater pulmonary i...
Journal of the American Veterinary Medical Association, 2020
OBJECTIVE To compare the cause of death (COD; whether by natural death or euthanasia for poor qua... more OBJECTIVE To compare the cause of death (COD; whether by natural death or euthanasia for poor quality of life caused by a primary pathological condition) between search-and-rescue (SAR) dogs deployed to the World Trade Center, Pentagon, or Fresh Kills Landfill on Staten Island following the 9/11 terrorist attacks and SAR dogs that were not deployed to these sites. ANIMALS 95 deployed SAR dogs (exposed dogs) and 55 nondeployed SAR dogs (unexposed dogs). PROCEDURES Following natural death or euthanasia, 63 dogs (44 exposed and 19 unexposed) underwent a necropsy examination. For the remaining 87 dogs, the COD was categorized on the basis of information obtained from medical records or personal communications. RESULTS The median age of death was 12.8 years for exposed dogs and 12.7 years for unexposed dogs. The COD was not impacted by deployment status. In the 150 exposed and unexposed dogs, degenerative conditions were the most common COD followed by neoplasia. Respiratory disease was ...
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 2018
A 7-y-old Weimaraner bitch was presented to emergency service after 3 h of active labor with no p... more A 7-y-old Weimaraner bitch was presented to emergency service after 3 h of active labor with no puppies produced. Hemoabdomen and hemothorax were present at the time of surgery; prothrombin time (PT) and activated partial thromboplastin time (aPTT) were both found to be within normal ranges. Surgical cesarean section was performed; 4 dead puppies and 5 live puppies were delivered. Because hemostasis was difficult to achieve, a hysterectomy was performed; however, the dog died as the operation was being completed. At autopsy, the pleural cavity contained 1.5 L of unclotted blood; the peritoneal cavity was relatively normal, and no obvious hemorrhage was associated with the surgical sites. All 4 dead fetuses were opened, and their pleural cavities were filled with unclotted blood. An anticoagulant screen was performed, and brodifacoum was identified in the liver of the bitch. This case is unusual in that the PT and aPTT were within reference intervals, but brodifacoum was present in s...
We have previously reported that Δ-9-tetrahydrocannabinol (Δ9-THC)-treated mice challenged with i... more We have previously reported that Δ-9-tetrahydrocannabinol (Δ9-THC)-treated mice challenged with influenza virus A/PR/8/34 (PR8) developed increased viral hemagglutinin 1 (H1) mRNA levels and decreased monocyte and lymphocyte recruitment to the pulmonary airways when compared with mice challenged with PR8 alone. The objective of the present study was to examine the role of cannabinoid (CB1/CB2) receptors in mediating the effects of Δ9-THC on immune and epithelial cell responses to PR8. In the current study, Δ9-THC-treated CB1/CB2 receptor null (CB1−/−/CB2−/−) and wild-type mice infected with PR8 had marked increases in viral H1 mRNA when compared with CB1−/−/CB2−/− and wild-type mice challenged with PR8 alone. However, the magnitude of the H1 mRNA levels was greatly reduced in CB1−/−/CB2−/− mice as compared with wild-type mice. In addition, Δ9-THC-treated CB1−/−/CB2−/− mice infected with PR8 had increased CD4+ T cells and IFN-γ in bronchoalveolar lavage fluid with greater pulmonary i...
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Papers by John Buchweitz