Les carcinomes épidermoïdes de la cavité orale (CECO) représentent environ 25% des cancers de la ... more Les carcinomes épidermoïdes de la cavité orale (CECO) représentent environ 25% des cancers de la tête et du cou (HNSCC). Ils sont associés à un mauvais pronostic avec une survie à 10 ans n'excédant pas ~ 30%. Alors que le virus du papillome humain (HPV) est un facteur de risque reconnu dans les HNSCC, il n'est pas impliqué dans les CECO dont les principaux facteurs de risque sont liés à la consommation d'alcool et de tabac. Cependant, plusieurs auteurs notent une part croissante de CECO chez des patients non exposés à l’alcool et au tabac (facteurs de risque négatifs: RF-) et dont le statut HPV est négatif. Il est aujourd’hui reconnu que le microenvironnement immunitaire (TME) peut jouer un rôle majeur dans la progression et l'échappement tumoral. Dans notre travail, nous avons cherché à savoir si les CECO RF- sont épidémiologiquement différents des CECO RF+ (facteurs de risques positifs: RF+) et si la composition immunitaire du TME et des cellules circulantes diffèr...
Introduction: Lymphomatoid Papulosis (LyP) is a lymphoproliferative disorder with typical histopa... more Introduction: Lymphomatoid Papulosis (LyP) is a lymphoproliferative disorder with typical histopathological criteria and a spontaneous regression. Only few cases of oral involvement have been reported. We wondered about their clinical characteristics and their association with lymphoma. Method: This systematic review is conducted according to the PRISMA statement. The Oxford Centre for Evidence Based Medicine table (OCEBM) and the scoring from the National Agency for Accreditation and Assessment in Health (ANAES) was assigned to each study. Results: We have included 16 references. LyP can occur at any age with a female predilection. Oral lesions were unique ulcer and tongue was mostly affected. Histological types A, and C were dominant and LyP resolved spontaneously. 1 case was associated with a lymphoma. Discussion: Cutaneous LyP more affects men and are mostly classified as type A while those orals are type A or C. Oral LyP is probably a progressive lesion whose evolution is not i...
Background: Human conventional dendritic cells (cDC) are essential for the anti-tumor immune resp... more Background: Human conventional dendritic cells (cDC) are essential for the anti-tumor immune response. Their immunogenic and tolerogenic states in cancer remain controversial. Our objective was to define the range of DC activation states in vitro and to determine their relevance ex vivo in cancer. Methods: First, in vitro, we activated healthy donor cDC with 16 different stimuli and measured cDC and T cells cytokine and chemokine secretion upon co-culture (n = 130 individual experiments) to define new maturation archetypes. Second, we established the relevance of these archetypes in cancer, ex vivo in human. We performed in depth analysis of cDC infiltrating head and neck cancer (HNSCC) by flow cytometry (n = 22 patients), transcriptomic analysis (n = 6 patients), and cDC-enriched single-cell transcriptomics (ScRNAseq) (n = 10503 cells from 2 patients). Then we performed a merged analysis of cDC from in house and public ScRNAseq datasets (n = 36 tumor samples from HNSCC, Lung, and B...
Dendritic cells (DC) are traditionally classified according to their ontogeny and their ability t... more Dendritic cells (DC) are traditionally classified according to their ontogeny and their ability to induce T cell response to antigens, however, the phenotypic and functional state of these cells in cancer does not necessarily align to the conventional categories. Here we show, by using 16 different stimuli in vitro that activated DCs in human blood are phenotypically and functionally dichotomous, and pure cultures of type 2 conventional dendritic cells acquire these states (termed Secretory and Helper) upon appropriate stimuli. PD-L1highICOSLlow Secretory DCs produce large amounts of inflammatory cytokines and chemokines but induce very low levels of T helper (Th) cytokines following co-culturing with T cells. Conversely, PD-L1lowICOSLhigh Helper DCs produce low levels of secreted factors but induce high levels and a broad range of Th cytokines. Secretory DCs bear a single-cell transcriptomic signature indicative of mature migratory LAMP3+ DCs associated with cancer and inflammation...
Revue de Stomatologie, de Chirurgie Maxillo-faciale et de Chirurgie Orale, 2016
INTRODUCTION Human hairs are generally localized on the cutaneous part of the head, neck, torso, ... more INTRODUCTION Human hairs are generally localized on the cutaneous part of the head, neck, torso, armpits, pubis and limbs. Sometimes it can be found in an unusual localization and is then called heterotopic. OBSERVATION A 30-year-old man presented with a hair in the middle of the dorsum of the tongue. It was decided to perform an excision under local anesthesia. DISCUSSION Few reports exist that describe hair growing on mucosa. Only one other case has been published concerning the tongue.
IntroductionOral Squamous Cell Carcinomas (OSCC) are mostly related to tobacco consumption eventu... more IntroductionOral Squamous Cell Carcinomas (OSCC) are mostly related to tobacco consumption eventually associated to alcohol (Smoker/Drinker patients: SD), but 25-30% of the patients have no identified risk factors (Non-Smoker/Non-Drinker patients: NSND). We hypothesized that these patients have distinguishable immune profiles that could be useful for prognosis.Materials and MethodsCells present in immune tumor microenvironment (TME) and blood from 87 OSCC HPV-negative patients were analyzed using a multiparameter flow cytometry assay, in a prospective case-control study. Cytokine levels in tumor supernatants and blood were determined by a cytometric bead array (CBA) assay.ResultsNormal gingiva and blood from healthy donors (HD) were used as controls. A significant increase of granulocytes (p<0.05 for blood), of monocytes-macrophages (p<0.01 for blood) and of CD4+ T cells expressing CD45RO and CCR6 (p<0.001 for blood; p<0.0001 for TME) as well as higher levels of IL-6 (p&...
Les carcinomes epidermoides de la cavite orale (CECO) representent environ 25% des cancers de la ... more Les carcinomes epidermoides de la cavite orale (CECO) representent environ 25% des cancers de la tete et du cou (HNSCC). Ils sont associes a un mauvais pronostic avec une survie a 10 ans n'excedant pas ~ 30%. Alors que le virus du papillome humain (HPV) est un facteur de risque reconnu dans les HNSCC, il n'est pas implique dans les CECO dont les principaux facteurs de risque sont lies a la consommation d'alcool et de tabac. Cependant, plusieurs auteurs notent une part croissante de CECO chez des patients non exposes a l’alcool et au tabac (facteurs de risque negatifs: RF-) et dont le statut HPV est negatif. Il est aujourd’hui reconnu que le microenvironnement immunitaire (TME) peut jouer un role majeur dans la progression et l'echappement tumoral. Dans notre travail, nous avons cherche a savoir si les CECO RF- sont epidemiologiquement differents des CECO RF+ (facteurs de risques positifs: RF+) et si la composition immunitaire du TME et des cellules circulantes differ...
Introduction: Clinical photography is an important tool for clinical practice, training and resea... more Introduction: Clinical photography is an important tool for clinical practice, training and research frequently used across medical specialties. It may provide snapshot images of evolving clinical situations. The aim of this work was to record its contribution in the management of oral mucosal diseases and, to identify the methods of using this tool by carrying out a survey with practitioners specialized in oral pathologies. This allowed us to make recommendations for appropriate use of this tool. Material and methods: An online anonymous questionnaire was sent via email, to practitioners who were member of two associations of French specialists. Results: 51 oral medicine specialists completed the survey. Among them, 48 (94%) used photography in their daily practice. Lack of time or interest was the reasons given by the 3 practitioners who did not use photography. Pictures were mostly taken with smartphones, which provide precise and accurate images, and stored on personal or profes...
Les carcinomes épidermoïdes de la cavité orale (CECO) représentent environ 25% des cancers de la ... more Les carcinomes épidermoïdes de la cavité orale (CECO) représentent environ 25% des cancers de la tête et du cou (HNSCC). Ils sont associés à un mauvais pronostic avec une survie à 10 ans n'excédant pas ~ 30%. Alors que le virus du papillome humain (HPV) est un facteur de risque reconnu dans les HNSCC, il n'est pas impliqué dans les CECO dont les principaux facteurs de risque sont liés à la consommation d'alcool et de tabac. Cependant, plusieurs auteurs notent une part croissante de CECO chez des patients non exposés à l’alcool et au tabac (facteurs de risque négatifs: RF-) et dont le statut HPV est négatif. Il est aujourd’hui reconnu que le microenvironnement immunitaire (TME) peut jouer un rôle majeur dans la progression et l'échappement tumoral. Dans notre travail, nous avons cherché à savoir si les CECO RF- sont épidémiologiquement différents des CECO RF+ (facteurs de risques positifs: RF+) et si la composition immunitaire du TME et des cellules circulantes diffèr...
Introduction: Lymphomatoid Papulosis (LyP) is a lymphoproliferative disorder with typical histopa... more Introduction: Lymphomatoid Papulosis (LyP) is a lymphoproliferative disorder with typical histopathological criteria and a spontaneous regression. Only few cases of oral involvement have been reported. We wondered about their clinical characteristics and their association with lymphoma. Method: This systematic review is conducted according to the PRISMA statement. The Oxford Centre for Evidence Based Medicine table (OCEBM) and the scoring from the National Agency for Accreditation and Assessment in Health (ANAES) was assigned to each study. Results: We have included 16 references. LyP can occur at any age with a female predilection. Oral lesions were unique ulcer and tongue was mostly affected. Histological types A, and C were dominant and LyP resolved spontaneously. 1 case was associated with a lymphoma. Discussion: Cutaneous LyP more affects men and are mostly classified as type A while those orals are type A or C. Oral LyP is probably a progressive lesion whose evolution is not i...
Background: Human conventional dendritic cells (cDC) are essential for the anti-tumor immune resp... more Background: Human conventional dendritic cells (cDC) are essential for the anti-tumor immune response. Their immunogenic and tolerogenic states in cancer remain controversial. Our objective was to define the range of DC activation states in vitro and to determine their relevance ex vivo in cancer. Methods: First, in vitro, we activated healthy donor cDC with 16 different stimuli and measured cDC and T cells cytokine and chemokine secretion upon co-culture (n = 130 individual experiments) to define new maturation archetypes. Second, we established the relevance of these archetypes in cancer, ex vivo in human. We performed in depth analysis of cDC infiltrating head and neck cancer (HNSCC) by flow cytometry (n = 22 patients), transcriptomic analysis (n = 6 patients), and cDC-enriched single-cell transcriptomics (ScRNAseq) (n = 10503 cells from 2 patients). Then we performed a merged analysis of cDC from in house and public ScRNAseq datasets (n = 36 tumor samples from HNSCC, Lung, and B...
Dendritic cells (DC) are traditionally classified according to their ontogeny and their ability t... more Dendritic cells (DC) are traditionally classified according to their ontogeny and their ability to induce T cell response to antigens, however, the phenotypic and functional state of these cells in cancer does not necessarily align to the conventional categories. Here we show, by using 16 different stimuli in vitro that activated DCs in human blood are phenotypically and functionally dichotomous, and pure cultures of type 2 conventional dendritic cells acquire these states (termed Secretory and Helper) upon appropriate stimuli. PD-L1highICOSLlow Secretory DCs produce large amounts of inflammatory cytokines and chemokines but induce very low levels of T helper (Th) cytokines following co-culturing with T cells. Conversely, PD-L1lowICOSLhigh Helper DCs produce low levels of secreted factors but induce high levels and a broad range of Th cytokines. Secretory DCs bear a single-cell transcriptomic signature indicative of mature migratory LAMP3+ DCs associated with cancer and inflammation...
Revue de Stomatologie, de Chirurgie Maxillo-faciale et de Chirurgie Orale, 2016
INTRODUCTION Human hairs are generally localized on the cutaneous part of the head, neck, torso, ... more INTRODUCTION Human hairs are generally localized on the cutaneous part of the head, neck, torso, armpits, pubis and limbs. Sometimes it can be found in an unusual localization and is then called heterotopic. OBSERVATION A 30-year-old man presented with a hair in the middle of the dorsum of the tongue. It was decided to perform an excision under local anesthesia. DISCUSSION Few reports exist that describe hair growing on mucosa. Only one other case has been published concerning the tongue.
IntroductionOral Squamous Cell Carcinomas (OSCC) are mostly related to tobacco consumption eventu... more IntroductionOral Squamous Cell Carcinomas (OSCC) are mostly related to tobacco consumption eventually associated to alcohol (Smoker/Drinker patients: SD), but 25-30% of the patients have no identified risk factors (Non-Smoker/Non-Drinker patients: NSND). We hypothesized that these patients have distinguishable immune profiles that could be useful for prognosis.Materials and MethodsCells present in immune tumor microenvironment (TME) and blood from 87 OSCC HPV-negative patients were analyzed using a multiparameter flow cytometry assay, in a prospective case-control study. Cytokine levels in tumor supernatants and blood were determined by a cytometric bead array (CBA) assay.ResultsNormal gingiva and blood from healthy donors (HD) were used as controls. A significant increase of granulocytes (p<0.05 for blood), of monocytes-macrophages (p<0.01 for blood) and of CD4+ T cells expressing CD45RO and CCR6 (p<0.001 for blood; p<0.0001 for TME) as well as higher levels of IL-6 (p&...
Les carcinomes epidermoides de la cavite orale (CECO) representent environ 25% des cancers de la ... more Les carcinomes epidermoides de la cavite orale (CECO) representent environ 25% des cancers de la tete et du cou (HNSCC). Ils sont associes a un mauvais pronostic avec une survie a 10 ans n'excedant pas ~ 30%. Alors que le virus du papillome humain (HPV) est un facteur de risque reconnu dans les HNSCC, il n'est pas implique dans les CECO dont les principaux facteurs de risque sont lies a la consommation d'alcool et de tabac. Cependant, plusieurs auteurs notent une part croissante de CECO chez des patients non exposes a l’alcool et au tabac (facteurs de risque negatifs: RF-) et dont le statut HPV est negatif. Il est aujourd’hui reconnu que le microenvironnement immunitaire (TME) peut jouer un role majeur dans la progression et l'echappement tumoral. Dans notre travail, nous avons cherche a savoir si les CECO RF- sont epidemiologiquement differents des CECO RF+ (facteurs de risques positifs: RF+) et si la composition immunitaire du TME et des cellules circulantes differ...
Introduction: Clinical photography is an important tool for clinical practice, training and resea... more Introduction: Clinical photography is an important tool for clinical practice, training and research frequently used across medical specialties. It may provide snapshot images of evolving clinical situations. The aim of this work was to record its contribution in the management of oral mucosal diseases and, to identify the methods of using this tool by carrying out a survey with practitioners specialized in oral pathologies. This allowed us to make recommendations for appropriate use of this tool. Material and methods: An online anonymous questionnaire was sent via email, to practitioners who were member of two associations of French specialists. Results: 51 oral medicine specialists completed the survey. Among them, 48 (94%) used photography in their daily practice. Lack of time or interest was the reasons given by the 3 practitioners who did not use photography. Pictures were mostly taken with smartphones, which provide precise and accurate images, and stored on personal or profes...
Uploads
Papers by Juliette Rochefort