International Journal of Applied Biology and P, Jul 1, 2012
ABSTRACT: Menopause is the permanent cessation of mensturation due to loss of ovarian follicular ... more ABSTRACT: Menopause is the permanent cessation of mensturation due to loss of ovarian follicular function, which results in decreased production of estradiol and other hormones. Decreased levels of estrogen leads to variations in lipid profile leading to atherosclerosis thus by increasing risk of coronary artery disease in post menopausal women. The present study was conducted to evaluate the relationship between serum estradiol and lipid profile status in postmenopausal women to assess risk for coronary artery disease. Study includes 70 subjects, 30 healthy premenopausal women (25-40yrs) and the healthy postmenopausal women in the age group of (55 – 65yrs).Serum estradiol and lipid profile status were estimated in both cases and controls. Reduced estradiol (P0.0001) in post menopausal women and p value is highly significant for total cholesterol, triglycerides, low density lipoproteins. Conclusion: Thus the study outlines that the low levels of estrogen exerts a significant effect on the plasma lipids and lipoprotein metabolism. The risk of coronary artery disease increases in women after menopause. Key words: TC-total cholesterol, TG-Triglycerides, HDL-High density lipoproteins, LDL-Low density lipoproteins, CHD-coronary heart disease.
Introduction: Polycystic ovary syndrome (PCOS) is the most common endocrinological disorder in wo... more Introduction: Polycystic ovary syndrome (PCOS) is the most common endocrinological disorder in women of reproductive age group. It is the leading cause of infertility in females presenting a wide range of clinical manifestations. Aim: To investigate the glycaemic status, calcium, phosphorus, insulin and 25-OH vitamin D levels in women with polycystic ovary syndrome and to compare these parameters with age and BMI matched healthy control women. To find out the correlation between insulin resistance and vitamin D among PCOS women. Materials and Methods: The study comprised of 80 newly diagnosed polycystic ovary syndrome women in the age range of 23 to 33 years. The biochemical parameters measured in the study includes fasting blood sugar, fasting insulin, calcium, phosphorus and vitamin D. The values obtained were compared with equal number of age and body mass indexed (BMI) matched healthy controls. Results and Discussion: In the present study the levels of fasting glucose, fasting i...
International Journal of Fertility and Sterility, Apr 5, 2016
Polycystic ovary syndrome (PCOS) is one of the most common endocrinological disorders among women... more Polycystic ovary syndrome (PCOS) is one of the most common endocrinological disorders among women of reproductive age and the leading cause of female infertility. This study intends to evaluate the lipid profile, hormonal levels [free T3 (fT3), free T4 (fT4), thyroid stimulating hormone (TSH), insulin, luteinizing hormone (LH), follicle stimulating hormone (FSH), and prolactin] in PCOS women from Nellore and its surrounding districts of Andhra Pradesh, India. This cross-sectional study included 80 newly diagnosed PCOS women and an equal number of age and body mass index (BMI) matched healthy controls. We used the photometry methods to determine serum glucose levels and the lipid profile. An immunoturbidometry method was employed to measure high sensitive C-reactive protein (hsCRP). All hormonal parameters were measured using chemiluminescence immunoassays. Insulin resistance was evaluated using the homeostatic model assessment-insulin resistance (HOMA-IR) method. Statistical analysi...
ABSTRACT: Background: The interplay between inflammation and lipids has recently been the focus o... more ABSTRACT: Background: The interplay between inflammation and lipids has recently been the focus of research aimed at understanding the development of Type 2 diabetes mellitus and the process of atherogenesis. Inflammatory markers particularly Tumor necrosis factor-α and high sensitive C-reactive protein interfere with lipid homeostasis and activates proinflammatory mechanisms in type-2 Diabetes mellitus. Our aim of the study was to analyze and correlate the inflammatory markers with lipids in Type2 Diabetes mellitus. 50 diabetic subjects and 50 normal healthy controls were recruited from the outpatient department of Narayana Medical college and hospital, Nellore, A.P). Serum TNF-alpha concentration was measured using Sandwich ELISA kit method. commercial enzymatic methods were used in the determination of serum total.cholesterol, HDL cholesterol and Triacylglycerides. LDL cholesterol was estimated using friedewald equation. Turbidimetric immunoassay (QUANTIA CRP- US) is used for ult...
Uric acid, the end product of purine catabolism in humans and is known for its crystal deposition... more Uric acid, the end product of purine catabolism in humans and is known for its crystal deposition at higher concentrations (>7 mg/dl) in gout. Less is known about its antioxidant property and the beneficial effects in various diseases. It is thought that high concentration of uric acid in humans is an evolutionary advantage and it is also hypothesized that high concentration of uric acid is to compensate the antioxidant capacity of ascorbic acid which is lost in humans during the course of evolution. In the extracelluar environment, uric acid can scavenge free radicals like hydroxyl radical, singlet oxygen and peroxynitrite radical therefore, it is considered as a powerful antioxidant. On the other hand uric acid depending upon the chemical milieu, changing its property and at times it acts as pro oxidant and is associated with the pathobiochemistry in developing various diseases like hypertension, cardio vascular diseases, ischemia reperfusion injury, diabetes mellitus, non alco...
ABSTRACT: Osteoporosis is the term used for diseases that cause a reduction in the mass of bone p... more ABSTRACT: Osteoporosis is the term used for diseases that cause a reduction in the mass of bone perunit volume and is one of the dreaded afflictions of ageing. Osteoporosis increases bone fragility and susceptibility to fractures. This silently progressing metabolic bone disease is widely prevealent in India. This study was conducted to evaluate the role of biochemical bone turnover markers in postmenopausal women to assess the risk of osteoporosis. Study includes 70 women (40 postmenopausal, 30 premenopausal women) serum estradiol, serum alkaline phosphatase, urinary hydroxyproline were estimated in both cases and controls. There is decrease in serum estrodiaol levels in postmenopausal women when compared to premenopausal women estrogen deficiency induces bone loss similarly ALP is highly significant P<0.001 in prostmenopausal women suggesting that there is high alkaline phosphate activity in postmenopausal women as a result of the inhibitory effects of estrogen on bone turnover...
ABSTRACT: Menopause is the permanent cessation of mensturation due to loss of ovarian follicular ... more ABSTRACT: Menopause is the permanent cessation of mensturation due to loss of ovarian follicular function, which results in decreased production of estradiol and other hormones. Decreased levels of estrogen leads to variations in lipid profile leading to atherosclerosis thus by increasing risk of coronary artery disease in post menopausal women. The present study was conducted to evaluate the relationship between serum estradiol and lipid profile status in postmenopausal women to assess risk for coronary artery disease. Study includes 70 subjects, 30 healthy premenopausal women (25-40yrs) and the healthy postmenopausal women in the age group of (55 – 65yrs).Serum estradiol and lipid profile status were estimated in both cases and controls. Reduced estradiol (P0.0001) in post menopausal women and p value is highly significant for total cholesterol, triglycerides, low density lipoproteins. Conclusion: Thus the study outlines that the low levels of estrogen exerts a significant effect ...
International journal of pharma and bio sciences, 2015
The Incidence of Prostate Cancer is increasing day by day all over the world. At present the Inci... more The Incidence of Prostate Cancer is increasing day by day all over the world. At present the Incidence is about 5 per 1, 00,000 in India. There is strong evidence that genetic and environmental factors play in important role in pathophyology, of prostatic cancer. In this study HOMA-IR, serum Calcium, Phosphate, Lipid profile, PSA and antioxidant vitamins were estimated. Age matched 50 Prostate cancer cases and 50 controls included in the study. The insulin resistance in all cases and control groups evaluated by estimating fasting blood glucose and insulin levels, by using HOMA IR Formula. Antioxidant vitamins estimated by HPLC, Serum Calcium, Phosphate, lipid profile and Prostate specific antigen by enzymatic kits using Autoanlyzers. In the present study, the values of HOMA-IR, serum Calcium and PSA are (p<0.05) significantly increased than in controls. The Serum alfa tocopherol and ascorbic acid values in cases significantly reduced (p<0.05) than in controls. The growing of p...
Hypertensive disorders during pregnancy increase maternal and fetal risk. Preeclampsia is charact... more Hypertensive disorders during pregnancy increase maternal and fetal risk. Preeclampsia is characterised by hypertension and proteinuria. Increased uric acid is a key clinical feature of preeclampsia; higher levels correlate with significant maternal and fetal morbidity and mortality. The present study was undertaken to estimate serum uric acid , the end product of purine catabolism, in clinically diagnosed preeclamptic women (n=30) and the values were compared with that of normotensive pregnant women (n=30) ,aged 18-30yrs. All of them were in their third trimester and primigravida. Serum uric acid levels were measured by spectrophotometry. We observed that serum uric acid levels were increased significantly when compared with that of normotensive pregnant women (p value < 0.000).
Uric acid, the end product of purine catabolism in humans and is known for its crystal deposition... more Uric acid, the end product of purine catabolism in humans and is known for its crystal deposition at higher concentrations (>7 mg/dl) in gout. Less is known about its antioxidant property and the beneficial effects in various diseases. It is thought that high concentration of uric acid in humans is an evolutionary advantage and it is also hypothesized that high concentration of uric acid is to compensate the antioxidant capacity of ascorbic acid which is lost in humans during the course of evolution. In the extracelluar environment, uric acid can scavenge free radicals like hydroxyl radical, singlet oxygen and peroxynitrite radical therefore, it is considered as a powerful antioxidant. On the other hand uric acid depending upon the chemical milieu, changing its property and at times it acts as pro oxidant and is associated with the pathobiochemistry in developing various diseases like hypertension, cardio vascular diseases, ischemia reperfusion injury, diabetes mellitus, non alco...
International Journal of Clinical Biochemistry and Research, 2021
Essential hypertensionis a typical example of complex, multifactorial trait and a well studied ri... more Essential hypertensionis a typical example of complex, multifactorial trait and a well studied risk factor for cardiovascular disease. hs-CRP is well known marker of inflammation resulting in atherosclerosis and cardiovascular disease. Recent studies showed that 25-OH-vitamin-D has ananti-inflammatory role and decreased levels may increase the cardiovascular risk in subjects with essential hypertension. The study included 30 adults both male and female with essential hypertension (Systolic Blood Pressure ? 140 mm Hg and Diastolic Blood Pressure ? 90 mm Hg) as cases. 30 age and sex matched healthy individuals both male & female between the age group of 24-60 years attending General OPD, General Medicine Department, NMCH, were taken as controls. The inverse relationship of Vitamin D with hs-CRP levels leads to cardiovascular risk in subjects with essential hypertension. Keywords: Essential hypertension, hs-CRP, Vitamin D and Inflammation
Background: The interplay between inflammation and lipids has recently been the focus of research... more Background: The interplay between inflammation and lipids has recently been the focus of research aimed at understanding the development of Type 2 diabetes mellitus and the process of atherogenesis. Inflammatory markers particularly Tumor necrosis factor-α and high sensitive C-reactive protein interfere with lipid homeostasis and activates proinflammatory mechanisms in type-2 Diabetes mellitus. Our aim of the study was to analyze and correlate the inflammatory markers with lipids in Type2 Diabetes mellitus. 50 diabetic subjects and 50 normal healthy controls were recruited from the outpatient department of Narayana Medical college and hospital, Nellore, A.P). Serum TNF-alpha concentration was measured using Sandwich ELISA kit method. commercial enzymatic methods were used in the determination of serum total.cholesterol, HDL cholesterol and Triacylglycerides. LDL cholesterol was estimated using friedewald equation. Turbidimetric immunoassay (QUANTIA CRPUS) is used for ultrasensitive ...
Osteoporosis is the term used for diseases that cause a reduction in the mass of bone perunit vol... more Osteoporosis is the term used for diseases that cause a reduction in the mass of bone perunit volume and is one of the dreaded afflictions of ageing. Osteoporosis increases bone fragility and susceptibility to fractures. This silently progressing metabolic bone disease is widely prevealent in India. This study was conducted to evaluate the role of biochemical bone turnover markers in postmenopausal women to assess the risk of osteoporosis. Study includes 70 women (40 postmenopausal, 30 premenopausal women) serum estradiol, serum alkaline phosphatase, urinary hydroxyproline were estimated in both cases and controls. There is decrease in serum estrodiaol levels in postmenopausal women when compared to premenopausal women estrogen deficiency induces bone loss similarly ALP is highly significant P<0.001 in prostmenopausal women suggesting that there is high alkaline phosphate activity in postmenopausal women as a result of the inhibitory effects of estrogen on bone turnover rate whic...
International Journal of Applied Biology and P, 2011
Obesity and Insulin resistance are very frequent disorders and are described as the dominant risk... more Obesity and Insulin resistance are very frequent disorders and are described as the dominant risk factors for cardiovascular diseases. Recent research suggests that TNF-alpha, an adipocytokine plays a key role in the development of insulin resistance. Aim of the study was to estimate TNF-alpha levels and to investigate the association of TNF-alpha with the various factors associated with insulin resistance like Body mass index, Waist Hip Ratio, fasting insulin etc. A total of 100 subjects attending out patient department of General Medicine , Narayana Medical College , Nellore were selected for the study.50 patients were diagnosed as type-2 diabetics and 50 subjects were taken as normal healthy controls who attended for a general check-up. Anthropometric and biochemical measurements were analyzed. Serum Insulin and TNF-α were measured using chemiluminescence method and ELISA method respectively. Homeostasis model assessment score was used to gauge the level of insulin resistance. Ou...
The incidence of prostate cancer is 5 per 100000 in southern and eastern Asia. Both genetic and e... more The incidence of prostate cancer is 5 per 100000 in southern and eastern Asia. Both genetic and environmental factors have been implicated in its etiology. The mitogenic and growth stimulatory effects of Insulin growth factor may be involved in prostate carcinogenesis. To evaluate serum insulin and insulin resistance was passed by HOMA- IR. Prostatic specific antigen passed by immune-enzymatic assay. Vitamins were estimated by high performance liquid chromatography. In our study 30 prostate cancer patients aged 60-80years were taken as cases. 30 normal age matched disease free person were taken as controls in both groups, Insulin resistance and antioxidant vitamin status was studied. In the present study, the value of HOMA-IR was (P <0.05) is significantly higher compare to controls. Serum vitamin E and vitamin C values for cases was reduced (P <0.05) significantly lower than controls. The development of prostate cancer is a multistep process. Hyperinsulinemia associated with ...
Cigarette smoking is one of the major health hazards of the world population as it is contributin... more Cigarette smoking is one of the major health hazards of the world population as it is contributing to the cardiovascular diseases and to the increasing proportion of the sudden deaths. hsCRP and Fibrinogen are independent & signifigant markers of cardiovascular mortality. The aim of the study is to identify the earlier markers of atherogenesis in healthy adult male smokers for better risk prediction and screening purpose. 60 adult healthy men of age group 19-49 years were enrolled. 30 smokers & 30 nonsmoker. Smokers were compared with the non-smokers by doing the blood tests which included blood glucose, lipidprofile, hsCRP & Fibrinogen. Smokers had significantly higher levels of total serum cholesterol [245.03±72.82 vs160.07±21.39 p<0.000], LDL levels[149.17±48.87vs.98.67±14.93p<0.000] and triglyceride levels [152.43± 52.40vs. 94.83±17.75p<0.000]. HDL levels were low in smokers. hsCRP & Fibrinogen levels were higher in smokers [6.8±3.04vs1.38±0.67p<0.00&644.20±231.38vs3...
International Journal of Applied Biology and P, Jul 1, 2012
ABSTRACT: Menopause is the permanent cessation of mensturation due to loss of ovarian follicular ... more ABSTRACT: Menopause is the permanent cessation of mensturation due to loss of ovarian follicular function, which results in decreased production of estradiol and other hormones. Decreased levels of estrogen leads to variations in lipid profile leading to atherosclerosis thus by increasing risk of coronary artery disease in post menopausal women. The present study was conducted to evaluate the relationship between serum estradiol and lipid profile status in postmenopausal women to assess risk for coronary artery disease. Study includes 70 subjects, 30 healthy premenopausal women (25-40yrs) and the healthy postmenopausal women in the age group of (55 – 65yrs).Serum estradiol and lipid profile status were estimated in both cases and controls. Reduced estradiol (P0.0001) in post menopausal women and p value is highly significant for total cholesterol, triglycerides, low density lipoproteins. Conclusion: Thus the study outlines that the low levels of estrogen exerts a significant effect on the plasma lipids and lipoprotein metabolism. The risk of coronary artery disease increases in women after menopause. Key words: TC-total cholesterol, TG-Triglycerides, HDL-High density lipoproteins, LDL-Low density lipoproteins, CHD-coronary heart disease.
Introduction: Polycystic ovary syndrome (PCOS) is the most common endocrinological disorder in wo... more Introduction: Polycystic ovary syndrome (PCOS) is the most common endocrinological disorder in women of reproductive age group. It is the leading cause of infertility in females presenting a wide range of clinical manifestations. Aim: To investigate the glycaemic status, calcium, phosphorus, insulin and 25-OH vitamin D levels in women with polycystic ovary syndrome and to compare these parameters with age and BMI matched healthy control women. To find out the correlation between insulin resistance and vitamin D among PCOS women. Materials and Methods: The study comprised of 80 newly diagnosed polycystic ovary syndrome women in the age range of 23 to 33 years. The biochemical parameters measured in the study includes fasting blood sugar, fasting insulin, calcium, phosphorus and vitamin D. The values obtained were compared with equal number of age and body mass indexed (BMI) matched healthy controls. Results and Discussion: In the present study the levels of fasting glucose, fasting i...
International Journal of Fertility and Sterility, Apr 5, 2016
Polycystic ovary syndrome (PCOS) is one of the most common endocrinological disorders among women... more Polycystic ovary syndrome (PCOS) is one of the most common endocrinological disorders among women of reproductive age and the leading cause of female infertility. This study intends to evaluate the lipid profile, hormonal levels [free T3 (fT3), free T4 (fT4), thyroid stimulating hormone (TSH), insulin, luteinizing hormone (LH), follicle stimulating hormone (FSH), and prolactin] in PCOS women from Nellore and its surrounding districts of Andhra Pradesh, India. This cross-sectional study included 80 newly diagnosed PCOS women and an equal number of age and body mass index (BMI) matched healthy controls. We used the photometry methods to determine serum glucose levels and the lipid profile. An immunoturbidometry method was employed to measure high sensitive C-reactive protein (hsCRP). All hormonal parameters were measured using chemiluminescence immunoassays. Insulin resistance was evaluated using the homeostatic model assessment-insulin resistance (HOMA-IR) method. Statistical analysi...
ABSTRACT: Background: The interplay between inflammation and lipids has recently been the focus o... more ABSTRACT: Background: The interplay between inflammation and lipids has recently been the focus of research aimed at understanding the development of Type 2 diabetes mellitus and the process of atherogenesis. Inflammatory markers particularly Tumor necrosis factor-α and high sensitive C-reactive protein interfere with lipid homeostasis and activates proinflammatory mechanisms in type-2 Diabetes mellitus. Our aim of the study was to analyze and correlate the inflammatory markers with lipids in Type2 Diabetes mellitus. 50 diabetic subjects and 50 normal healthy controls were recruited from the outpatient department of Narayana Medical college and hospital, Nellore, A.P). Serum TNF-alpha concentration was measured using Sandwich ELISA kit method. commercial enzymatic methods were used in the determination of serum total.cholesterol, HDL cholesterol and Triacylglycerides. LDL cholesterol was estimated using friedewald equation. Turbidimetric immunoassay (QUANTIA CRP- US) is used for ult...
Uric acid, the end product of purine catabolism in humans and is known for its crystal deposition... more Uric acid, the end product of purine catabolism in humans and is known for its crystal deposition at higher concentrations (>7 mg/dl) in gout. Less is known about its antioxidant property and the beneficial effects in various diseases. It is thought that high concentration of uric acid in humans is an evolutionary advantage and it is also hypothesized that high concentration of uric acid is to compensate the antioxidant capacity of ascorbic acid which is lost in humans during the course of evolution. In the extracelluar environment, uric acid can scavenge free radicals like hydroxyl radical, singlet oxygen and peroxynitrite radical therefore, it is considered as a powerful antioxidant. On the other hand uric acid depending upon the chemical milieu, changing its property and at times it acts as pro oxidant and is associated with the pathobiochemistry in developing various diseases like hypertension, cardio vascular diseases, ischemia reperfusion injury, diabetes mellitus, non alco...
ABSTRACT: Osteoporosis is the term used for diseases that cause a reduction in the mass of bone p... more ABSTRACT: Osteoporosis is the term used for diseases that cause a reduction in the mass of bone perunit volume and is one of the dreaded afflictions of ageing. Osteoporosis increases bone fragility and susceptibility to fractures. This silently progressing metabolic bone disease is widely prevealent in India. This study was conducted to evaluate the role of biochemical bone turnover markers in postmenopausal women to assess the risk of osteoporosis. Study includes 70 women (40 postmenopausal, 30 premenopausal women) serum estradiol, serum alkaline phosphatase, urinary hydroxyproline were estimated in both cases and controls. There is decrease in serum estrodiaol levels in postmenopausal women when compared to premenopausal women estrogen deficiency induces bone loss similarly ALP is highly significant P<0.001 in prostmenopausal women suggesting that there is high alkaline phosphate activity in postmenopausal women as a result of the inhibitory effects of estrogen on bone turnover...
ABSTRACT: Menopause is the permanent cessation of mensturation due to loss of ovarian follicular ... more ABSTRACT: Menopause is the permanent cessation of mensturation due to loss of ovarian follicular function, which results in decreased production of estradiol and other hormones. Decreased levels of estrogen leads to variations in lipid profile leading to atherosclerosis thus by increasing risk of coronary artery disease in post menopausal women. The present study was conducted to evaluate the relationship between serum estradiol and lipid profile status in postmenopausal women to assess risk for coronary artery disease. Study includes 70 subjects, 30 healthy premenopausal women (25-40yrs) and the healthy postmenopausal women in the age group of (55 – 65yrs).Serum estradiol and lipid profile status were estimated in both cases and controls. Reduced estradiol (P0.0001) in post menopausal women and p value is highly significant for total cholesterol, triglycerides, low density lipoproteins. Conclusion: Thus the study outlines that the low levels of estrogen exerts a significant effect ...
International journal of pharma and bio sciences, 2015
The Incidence of Prostate Cancer is increasing day by day all over the world. At present the Inci... more The Incidence of Prostate Cancer is increasing day by day all over the world. At present the Incidence is about 5 per 1, 00,000 in India. There is strong evidence that genetic and environmental factors play in important role in pathophyology, of prostatic cancer. In this study HOMA-IR, serum Calcium, Phosphate, Lipid profile, PSA and antioxidant vitamins were estimated. Age matched 50 Prostate cancer cases and 50 controls included in the study. The insulin resistance in all cases and control groups evaluated by estimating fasting blood glucose and insulin levels, by using HOMA IR Formula. Antioxidant vitamins estimated by HPLC, Serum Calcium, Phosphate, lipid profile and Prostate specific antigen by enzymatic kits using Autoanlyzers. In the present study, the values of HOMA-IR, serum Calcium and PSA are (p<0.05) significantly increased than in controls. The Serum alfa tocopherol and ascorbic acid values in cases significantly reduced (p<0.05) than in controls. The growing of p...
Hypertensive disorders during pregnancy increase maternal and fetal risk. Preeclampsia is charact... more Hypertensive disorders during pregnancy increase maternal and fetal risk. Preeclampsia is characterised by hypertension and proteinuria. Increased uric acid is a key clinical feature of preeclampsia; higher levels correlate with significant maternal and fetal morbidity and mortality. The present study was undertaken to estimate serum uric acid , the end product of purine catabolism, in clinically diagnosed preeclamptic women (n=30) and the values were compared with that of normotensive pregnant women (n=30) ,aged 18-30yrs. All of them were in their third trimester and primigravida. Serum uric acid levels were measured by spectrophotometry. We observed that serum uric acid levels were increased significantly when compared with that of normotensive pregnant women (p value < 0.000).
Uric acid, the end product of purine catabolism in humans and is known for its crystal deposition... more Uric acid, the end product of purine catabolism in humans and is known for its crystal deposition at higher concentrations (>7 mg/dl) in gout. Less is known about its antioxidant property and the beneficial effects in various diseases. It is thought that high concentration of uric acid in humans is an evolutionary advantage and it is also hypothesized that high concentration of uric acid is to compensate the antioxidant capacity of ascorbic acid which is lost in humans during the course of evolution. In the extracelluar environment, uric acid can scavenge free radicals like hydroxyl radical, singlet oxygen and peroxynitrite radical therefore, it is considered as a powerful antioxidant. On the other hand uric acid depending upon the chemical milieu, changing its property and at times it acts as pro oxidant and is associated with the pathobiochemistry in developing various diseases like hypertension, cardio vascular diseases, ischemia reperfusion injury, diabetes mellitus, non alco...
International Journal of Clinical Biochemistry and Research, 2021
Essential hypertensionis a typical example of complex, multifactorial trait and a well studied ri... more Essential hypertensionis a typical example of complex, multifactorial trait and a well studied risk factor for cardiovascular disease. hs-CRP is well known marker of inflammation resulting in atherosclerosis and cardiovascular disease. Recent studies showed that 25-OH-vitamin-D has ananti-inflammatory role and decreased levels may increase the cardiovascular risk in subjects with essential hypertension. The study included 30 adults both male and female with essential hypertension (Systolic Blood Pressure ? 140 mm Hg and Diastolic Blood Pressure ? 90 mm Hg) as cases. 30 age and sex matched healthy individuals both male & female between the age group of 24-60 years attending General OPD, General Medicine Department, NMCH, were taken as controls. The inverse relationship of Vitamin D with hs-CRP levels leads to cardiovascular risk in subjects with essential hypertension. Keywords: Essential hypertension, hs-CRP, Vitamin D and Inflammation
Background: The interplay between inflammation and lipids has recently been the focus of research... more Background: The interplay between inflammation and lipids has recently been the focus of research aimed at understanding the development of Type 2 diabetes mellitus and the process of atherogenesis. Inflammatory markers particularly Tumor necrosis factor-α and high sensitive C-reactive protein interfere with lipid homeostasis and activates proinflammatory mechanisms in type-2 Diabetes mellitus. Our aim of the study was to analyze and correlate the inflammatory markers with lipids in Type2 Diabetes mellitus. 50 diabetic subjects and 50 normal healthy controls were recruited from the outpatient department of Narayana Medical college and hospital, Nellore, A.P). Serum TNF-alpha concentration was measured using Sandwich ELISA kit method. commercial enzymatic methods were used in the determination of serum total.cholesterol, HDL cholesterol and Triacylglycerides. LDL cholesterol was estimated using friedewald equation. Turbidimetric immunoassay (QUANTIA CRPUS) is used for ultrasensitive ...
Osteoporosis is the term used for diseases that cause a reduction in the mass of bone perunit vol... more Osteoporosis is the term used for diseases that cause a reduction in the mass of bone perunit volume and is one of the dreaded afflictions of ageing. Osteoporosis increases bone fragility and susceptibility to fractures. This silently progressing metabolic bone disease is widely prevealent in India. This study was conducted to evaluate the role of biochemical bone turnover markers in postmenopausal women to assess the risk of osteoporosis. Study includes 70 women (40 postmenopausal, 30 premenopausal women) serum estradiol, serum alkaline phosphatase, urinary hydroxyproline were estimated in both cases and controls. There is decrease in serum estrodiaol levels in postmenopausal women when compared to premenopausal women estrogen deficiency induces bone loss similarly ALP is highly significant P<0.001 in prostmenopausal women suggesting that there is high alkaline phosphate activity in postmenopausal women as a result of the inhibitory effects of estrogen on bone turnover rate whic...
International Journal of Applied Biology and P, 2011
Obesity and Insulin resistance are very frequent disorders and are described as the dominant risk... more Obesity and Insulin resistance are very frequent disorders and are described as the dominant risk factors for cardiovascular diseases. Recent research suggests that TNF-alpha, an adipocytokine plays a key role in the development of insulin resistance. Aim of the study was to estimate TNF-alpha levels and to investigate the association of TNF-alpha with the various factors associated with insulin resistance like Body mass index, Waist Hip Ratio, fasting insulin etc. A total of 100 subjects attending out patient department of General Medicine , Narayana Medical College , Nellore were selected for the study.50 patients were diagnosed as type-2 diabetics and 50 subjects were taken as normal healthy controls who attended for a general check-up. Anthropometric and biochemical measurements were analyzed. Serum Insulin and TNF-α were measured using chemiluminescence method and ELISA method respectively. Homeostasis model assessment score was used to gauge the level of insulin resistance. Ou...
The incidence of prostate cancer is 5 per 100000 in southern and eastern Asia. Both genetic and e... more The incidence of prostate cancer is 5 per 100000 in southern and eastern Asia. Both genetic and environmental factors have been implicated in its etiology. The mitogenic and growth stimulatory effects of Insulin growth factor may be involved in prostate carcinogenesis. To evaluate serum insulin and insulin resistance was passed by HOMA- IR. Prostatic specific antigen passed by immune-enzymatic assay. Vitamins were estimated by high performance liquid chromatography. In our study 30 prostate cancer patients aged 60-80years were taken as cases. 30 normal age matched disease free person were taken as controls in both groups, Insulin resistance and antioxidant vitamin status was studied. In the present study, the value of HOMA-IR was (P <0.05) is significantly higher compare to controls. Serum vitamin E and vitamin C values for cases was reduced (P <0.05) significantly lower than controls. The development of prostate cancer is a multistep process. Hyperinsulinemia associated with ...
Cigarette smoking is one of the major health hazards of the world population as it is contributin... more Cigarette smoking is one of the major health hazards of the world population as it is contributing to the cardiovascular diseases and to the increasing proportion of the sudden deaths. hsCRP and Fibrinogen are independent & signifigant markers of cardiovascular mortality. The aim of the study is to identify the earlier markers of atherogenesis in healthy adult male smokers for better risk prediction and screening purpose. 60 adult healthy men of age group 19-49 years were enrolled. 30 smokers & 30 nonsmoker. Smokers were compared with the non-smokers by doing the blood tests which included blood glucose, lipidprofile, hsCRP & Fibrinogen. Smokers had significantly higher levels of total serum cholesterol [245.03±72.82 vs160.07±21.39 p<0.000], LDL levels[149.17±48.87vs.98.67±14.93p<0.000] and triglyceride levels [152.43± 52.40vs. 94.83±17.75p<0.000]. HDL levels were low in smokers. hsCRP & Fibrinogen levels were higher in smokers [6.8±3.04vs1.38±0.67p<0.00&644.20±231.38vs3...
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