Recent studies have shown that propolis has appreciable antibacterial, antifungal and antiviral a... more Recent studies have shown that propolis has appreciable antibacterial, antifungal and antiviral actions, as well as cytostatic and antitumoral activity. In light of these studies, the antimicrobial activity of a new adhesive formulation containing propolis was evaluated in this in vitro study. Susceptibility of the oral strains tested (Candida albicans, Candida tropicalis, Streptococcus mutans, Staphylococcus aureus, Enterococcus faecalis, Actinomyces israelli, Actinobacillus actinomycetemcomitans) was evaluated using the agar diffusion method with different concentrations of propolis (5, 10, 15 and 20%). All of the assayed bacteria and fungi species were susceptible to propolis, with minimum inhibitory concentrations (MIC) ranging from 1.75 to 14.0 µg/ml. The positive results suggest that propolis in this sustained release formulation should be further tested as an alternative therapy of infectious conditions of the oral cavity, such as denture stomatitis and periodontitis. However...
Revista de Ciencias Farmaceuticas Basica e Aplicada
An ethanolic extract of leaves from the tree Casearia sylvestris, known as guaçatonga in Brazil, ... more An ethanolic extract of leaves from the tree Casearia sylvestris, known as guaçatonga in Brazil, was tested for in vitro activity against oral pathogenic bacteria and fungi. The results showed susceptibility of all the microorganisms tested. This study suggests a potential use of ethanolic extract of C. sylvestris as a novel treatment of oral infectious conditions, such as denture stomatitis, periodontitis and dental caries. Keywords: Casearia sylvestris; guaçatonga; oral microorganisms; antimicrobial activity.
The purpose of this in vitro study was to evaluate the antimicrobial effect of photodynamic thera... more The purpose of this in vitro study was to evaluate the antimicrobial effect of photodynamic therapy on Streptococcus mutans (A TCC 25175) suspensions, using a red laser for one minute in combination with toluidine blue O (TBO) or methylene blue (MB). Both photosensitizers were used in three concentrations (25, 10 and 5 mg/L). The activity ofphotosensitizers and laser irradiation were tested separately on the bacteria, as well as the irradiation of this light source in the presence of the TBO or MB. These groups were compared to a control group, in which the microorganism did not receive any treatment. The activity of both TBO and MB or laser irradiation, alone, were not able to reduce the number of S. mutans. In the groups of lethal photosensitization, a bacterial reduction of 70% for TBO and 73% for MB was observed when these photosensitizers were used at 25 mg/L and a reduction of 48% was observed for MB at 5mg/L. In other concentrations there were no significant differences in co...
... A. Gala-García, KIR Teixeira, LL Mendes, APR Sobrinho, VR Santos and ME Cortes ... In the cur... more ... A. Gala-García, KIR Teixeira, LL Mendes, APR Sobrinho, VR Santos and ME Cortes ... In the current study, the pulpal response after capping at the first day corroborates the results obtained with Ca(OH) 2 by other authors (Holland et al., 1978; Estrela & Pesce, 1996). ...
Journal of Inclusion Phenomena and Macrocyclic Chemistry, 2009
Page 1. ORIGINAL ARTICLE Inclusion complex of usnic acid with b-cyclodextrin: characterization an... more Page 1. ORIGINAL ARTICLE Inclusion complex of usnic acid with b-cyclodextrin: characterization and nanoencapsulation into liposomes Mariane CB Lira Æ Milena S. Ferraz Æ Dáfila GVC da Silva Æ Maria E. Cortes Æ Karina ...
Chemical hosts bind their guests by the same physical mechanisms as biomolecules and often displa... more Chemical hosts bind their guests by the same physical mechanisms as biomolecules and often display similarly subtle structure activity relationships. The cyclodextrins have found increasing application as inert, nontoxic carriers of active compounds in drug formulations. The present study was conducted to prepare inclusion complexes of chlorhexidine:β-cyclodextrin (Cx:β-cd), and evaluate their interactions with bacterial membrane through: scanning electron microscopy (SEM) and transmission electron microscopy (TEM); and measuring morphology alterations, roughness values, and cell weights by atomic force microscopy (AFM). It was found that the antimicrobial activity was significantly enhanced by cyclodextrin encapsulation. SEM analysis images demonstrated recognizable cell membrane structural changes and ultrastructural membrane swelling. By TEM, cellular alterations such as vacuolization, cellular leakage, and membrane defects were observed; these effects were enhanced at 1:3 and 1:...
The supramolecular assembly between chlorhexidine and cyclomaltoheptaose (beta-cyclodextrin, beta... more The supramolecular assembly between chlorhexidine and cyclomaltoheptaose (beta-cyclodextrin, betaCD) was characterized using NMR spectroscopy ((1)H, T(1), and ROESY), ESIMS and ITC. NMR data suggest the formation of high ordered complexes. ESIMS and ITC allowed the confirmation of the average stoichiometry as 1:4 and the thermodynamic data, also obtained by ITC, showed that the assembly is strongly stabilized by short distance interactions, but suffers a strong, opposite effect of entropy reduction. The antimicrobial activity of 1:1, 1:2, 1:3, and 1:4 Clx/betaCD molar ratio mixtures was investigated in aqueous solution and after incorporation into mucoadhesive gels. These were used to determine the initial and the long-term antimicrobial activity, respectively, toward Actinobacillus actinomycetemcomitans (A.a.) (Y4-FDC) and Enterococcus faecalis (E.f.) (ATCC 14508) strains. The results showed that A.a. and E.f. were more susceptible to the 1:4 molar ratio mixture in either solution or gel (p<0.05).
A presença do biofilme na cavidade oral é o principal fator etiológico da cárie dental. Diversas ... more A presença do biofilme na cavidade oral é o principal fator etiológico da cárie dental. Diversas teorias têm sido levantadas sobre a participação específica das bactérias na produção do ácido e na relação causal destas com essa doença. Contudo, existe consenso que além da ...
Recent studies have shown that propolis has appreciable antibacterial, antifungal and antiviral a... more Recent studies have shown that propolis has appreciable antibacterial, antifungal and antiviral actions, as well as cytostatic and antitumoral activity. In light of these studies, the antimicrobial activity of a new adhesive formulation containing propolis was evaluated in this in vitro study. Susceptibility of the oral strains tested (Candida albicans, Candida tropicalis, Streptococcus mutans, Staphylococcus aureus, Enterococcus faecalis, Actinomyces israelli, Actinobacillus actinomycetemcomitans) was evaluated using the agar diffusion method with different concentrations of propolis (5, 10, 15 and 20%). All of the assayed bacteria and fungi species were susceptible to propolis, with minimum inhibitory concentrations (MIC) ranging from 1.75 to 14.0 µg/ml. The positive results suggest that propolis in this sustained release formulation should be further tested as an alternative therapy of infectious conditions of the oral cavity, such as denture stomatitis and periodontitis. However...
Revista de Ciencias Farmaceuticas Basica e Aplicada
An ethanolic extract of leaves from the tree Casearia sylvestris, known as guaçatonga in Brazil, ... more An ethanolic extract of leaves from the tree Casearia sylvestris, known as guaçatonga in Brazil, was tested for in vitro activity against oral pathogenic bacteria and fungi. The results showed susceptibility of all the microorganisms tested. This study suggests a potential use of ethanolic extract of C. sylvestris as a novel treatment of oral infectious conditions, such as denture stomatitis, periodontitis and dental caries. Keywords: Casearia sylvestris; guaçatonga; oral microorganisms; antimicrobial activity.
The purpose of this in vitro study was to evaluate the antimicrobial effect of photodynamic thera... more The purpose of this in vitro study was to evaluate the antimicrobial effect of photodynamic therapy on Streptococcus mutans (A TCC 25175) suspensions, using a red laser for one minute in combination with toluidine blue O (TBO) or methylene blue (MB). Both photosensitizers were used in three concentrations (25, 10 and 5 mg/L). The activity ofphotosensitizers and laser irradiation were tested separately on the bacteria, as well as the irradiation of this light source in the presence of the TBO or MB. These groups were compared to a control group, in which the microorganism did not receive any treatment. The activity of both TBO and MB or laser irradiation, alone, were not able to reduce the number of S. mutans. In the groups of lethal photosensitization, a bacterial reduction of 70% for TBO and 73% for MB was observed when these photosensitizers were used at 25 mg/L and a reduction of 48% was observed for MB at 5mg/L. In other concentrations there were no significant differences in co...
... A. Gala-García, KIR Teixeira, LL Mendes, APR Sobrinho, VR Santos and ME Cortes ... In the cur... more ... A. Gala-García, KIR Teixeira, LL Mendes, APR Sobrinho, VR Santos and ME Cortes ... In the current study, the pulpal response after capping at the first day corroborates the results obtained with Ca(OH) 2 by other authors (Holland et al., 1978; Estrela & Pesce, 1996). ...
Journal of Inclusion Phenomena and Macrocyclic Chemistry, 2009
Page 1. ORIGINAL ARTICLE Inclusion complex of usnic acid with b-cyclodextrin: characterization an... more Page 1. ORIGINAL ARTICLE Inclusion complex of usnic acid with b-cyclodextrin: characterization and nanoencapsulation into liposomes Mariane CB Lira Æ Milena S. Ferraz Æ Dáfila GVC da Silva Æ Maria E. Cortes Æ Karina ...
Chemical hosts bind their guests by the same physical mechanisms as biomolecules and often displa... more Chemical hosts bind their guests by the same physical mechanisms as biomolecules and often display similarly subtle structure activity relationships. The cyclodextrins have found increasing application as inert, nontoxic carriers of active compounds in drug formulations. The present study was conducted to prepare inclusion complexes of chlorhexidine:β-cyclodextrin (Cx:β-cd), and evaluate their interactions with bacterial membrane through: scanning electron microscopy (SEM) and transmission electron microscopy (TEM); and measuring morphology alterations, roughness values, and cell weights by atomic force microscopy (AFM). It was found that the antimicrobial activity was significantly enhanced by cyclodextrin encapsulation. SEM analysis images demonstrated recognizable cell membrane structural changes and ultrastructural membrane swelling. By TEM, cellular alterations such as vacuolization, cellular leakage, and membrane defects were observed; these effects were enhanced at 1:3 and 1:...
The supramolecular assembly between chlorhexidine and cyclomaltoheptaose (beta-cyclodextrin, beta... more The supramolecular assembly between chlorhexidine and cyclomaltoheptaose (beta-cyclodextrin, betaCD) was characterized using NMR spectroscopy ((1)H, T(1), and ROESY), ESIMS and ITC. NMR data suggest the formation of high ordered complexes. ESIMS and ITC allowed the confirmation of the average stoichiometry as 1:4 and the thermodynamic data, also obtained by ITC, showed that the assembly is strongly stabilized by short distance interactions, but suffers a strong, opposite effect of entropy reduction. The antimicrobial activity of 1:1, 1:2, 1:3, and 1:4 Clx/betaCD molar ratio mixtures was investigated in aqueous solution and after incorporation into mucoadhesive gels. These were used to determine the initial and the long-term antimicrobial activity, respectively, toward Actinobacillus actinomycetemcomitans (A.a.) (Y4-FDC) and Enterococcus faecalis (E.f.) (ATCC 14508) strains. The results showed that A.a. and E.f. were more susceptible to the 1:4 molar ratio mixture in either solution or gel (p<0.05).
A presença do biofilme na cavidade oral é o principal fator etiológico da cárie dental. Diversas ... more A presença do biofilme na cavidade oral é o principal fator etiológico da cárie dental. Diversas teorias têm sido levantadas sobre a participação específica das bactérias na produção do ácido e na relação causal destas com essa doença. Contudo, existe consenso que além da ...
Uploads