Most shrimp disease challenge models have been developed and standardized for the study of diseas... more Most shrimp disease challenge models have been developed and standardized for the study of diseases caused by such specific viruses as White Spot Syndrome Virus and Taura Syndrome Virus. These challenge models are an important component of shrimp-breeding programs as an aid in disease-resistance selection. They are also used to evaluate the effects of environmental factors on the manifestation of the diseases and in the evaluation of possible therapeutic and preventative agents. Bacterial challenge models, in comparison, are less developed. Although very important, the economic impacts of bacterial diseases on the shrimp-farming industry are apparently not as severe as those of some viral diseases, so less interest and research funding are channeled in that direction. Additionally, bacterial challenge models are very difficult to standardize because shrimp's responses to infection are highly dependent on their physiological condition, the source of the shrimp, bacterial species ...
The most important diseases of farmed penaeid shrimp have infectious aetiologies. Among these are... more The most important diseases of farmed penaeid shrimp have infectious aetiologies. Among these are diseases with viral, rickettsial, bacterial, fungal and parasitic aetiologies. Diagnostic methods for these pathogens include the traditional methods of gross pathology, histopathology, classical microbiology, animal bioassay, antibody-based methods, and molecular methods using DNA probes and DNA amplification. While methods using clinical chemistry and tissue culture are standard methods in veterinary and human diagnostic laboratories, the former has not been routinely applied to the diagnosis of penaeid shrimp diseases and the latter has yet to be developed, despite considerable research and development efforts that have spanned the past 40 years. No continuous shrimp cell lines, or lines from other crustaceans, have been developed. Hence, when molecular methods began to be routinely applied to the diagnosis of infectious diseases in humans and domestic animals in the mid- to late 198...
Representative archived Litopenaeus vannamei samples (117 total), originating from 13 different c... more Representative archived Litopenaeus vannamei samples (117 total), originating from 13 different countries and collected between 1992 to 1996, were analyzed by in situ hybridization to verify the presence of Taura Syndrome Virus (TSV) within pathodiagnostic acute phase TS histological lesions. The in situ assay results showed that TSV was present in one or more representative samples analyzed from each country
ABSTRACT: Lesion development was documented in Penaeus vannamei juveniles with experimentally ind... more ABSTRACT: Lesion development was documented in Penaeus vannamei juveniles with experimentally induced, chronic phase Taura syndrome virus (TSV) infections, by both routine histology and in situ hybridization, during a 48 wk time course study. Histologically, the defining ...
... Re-cently, Mohney et al. ... Acknowledgments This work was primarily supported by funding fro... more ... Re-cently, Mohney et al. ... Acknowledgments This work was primarily supported by funding from the Center for Tropical and Subtropical Aquaculture (grant 91-38500-7262) and Abbott Laboratories. The technical advice and support of Brenda White are highly appreciated. ...
... He-matoxylin and eosin staining of paraffin-embedded fixed material from infected shrimp also... more ... He-matoxylin and eosin staining of paraffin-embedded fixed material from infected shrimp also showed multifocal granulomatous lesions within the lym-phoid organ, heart, and intertubular connective tis-sue of the hepatopancreas. ... 25 m). Page 4. 86 MOHNEY ET AL. ...
White spot syndrome virus (WSSV) is highly virulent to penaeid shrimp and has been responsible fo... more White spot syndrome virus (WSSV) is highly virulent to penaeid shrimp and has been responsible for serious economic losses on shrimp farms throughout the world. Infectious hypodermal and hematopoietic necrosis virus (IHHNV) is a small DNA virus that also ...
Most shrimp disease challenge models have been developed and standardized for the study of diseas... more Most shrimp disease challenge models have been developed and standardized for the study of diseases caused by such specific viruses as White Spot Syndrome Virus and Taura Syndrome Virus. These challenge models are an important component of shrimp-breeding programs as an aid in disease-resistance selection. They are also used to evaluate the effects of environmental factors on the manifestation of the diseases and in the evaluation of possible therapeutic and preventative agents. Bacterial challenge models, in comparison, are less developed. Although very important, the economic impacts of bacterial diseases on the shrimp-farming industry are apparently not as severe as those of some viral diseases, so less interest and research funding are channeled in that direction. Additionally, bacterial challenge models are very difficult to standardize because shrimp's responses to infection are highly dependent on their physiological condition, the source of the shrimp, bacterial species ...
The most important diseases of farmed penaeid shrimp have infectious aetiologies. Among these are... more The most important diseases of farmed penaeid shrimp have infectious aetiologies. Among these are diseases with viral, rickettsial, bacterial, fungal and parasitic aetiologies. Diagnostic methods for these pathogens include the traditional methods of gross pathology, histopathology, classical microbiology, animal bioassay, antibody-based methods, and molecular methods using DNA probes and DNA amplification. While methods using clinical chemistry and tissue culture are standard methods in veterinary and human diagnostic laboratories, the former has not been routinely applied to the diagnosis of penaeid shrimp diseases and the latter has yet to be developed, despite considerable research and development efforts that have spanned the past 40 years. No continuous shrimp cell lines, or lines from other crustaceans, have been developed. Hence, when molecular methods began to be routinely applied to the diagnosis of infectious diseases in humans and domestic animals in the mid- to late 198...
Representative archived Litopenaeus vannamei samples (117 total), originating from 13 different c... more Representative archived Litopenaeus vannamei samples (117 total), originating from 13 different countries and collected between 1992 to 1996, were analyzed by in situ hybridization to verify the presence of Taura Syndrome Virus (TSV) within pathodiagnostic acute phase TS histological lesions. The in situ assay results showed that TSV was present in one or more representative samples analyzed from each country
ABSTRACT: Lesion development was documented in Penaeus vannamei juveniles with experimentally ind... more ABSTRACT: Lesion development was documented in Penaeus vannamei juveniles with experimentally induced, chronic phase Taura syndrome virus (TSV) infections, by both routine histology and in situ hybridization, during a 48 wk time course study. Histologically, the defining ...
... Re-cently, Mohney et al. ... Acknowledgments This work was primarily supported by funding fro... more ... Re-cently, Mohney et al. ... Acknowledgments This work was primarily supported by funding from the Center for Tropical and Subtropical Aquaculture (grant 91-38500-7262) and Abbott Laboratories. The technical advice and support of Brenda White are highly appreciated. ...
... He-matoxylin and eosin staining of paraffin-embedded fixed material from infected shrimp also... more ... He-matoxylin and eosin staining of paraffin-embedded fixed material from infected shrimp also showed multifocal granulomatous lesions within the lym-phoid organ, heart, and intertubular connective tis-sue of the hepatopancreas. ... 25 m). Page 4. 86 MOHNEY ET AL. ...
White spot syndrome virus (WSSV) is highly virulent to penaeid shrimp and has been responsible fo... more White spot syndrome virus (WSSV) is highly virulent to penaeid shrimp and has been responsible for serious economic losses on shrimp farms throughout the world. Infectious hypodermal and hematopoietic necrosis virus (IHHNV) is a small DNA virus that also ...
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