Taking into account the worrying scenario of water scarcity, it is essential to enable more effic... more Taking into account the worrying scenario of water scarcity, it is essential to enable more efficient technologies for wastewater treatment. Wastewater may be treated by conventional biological processes that remove pathogenic organisms, particulate and soluble organic compounds, and other components. However, secondary effluents from treatment plants may still contain toxic elements or high concentrations of inorganic nutrients (mainly nitrogen and phosphorus), which enable the growth of photosynthetic microorganisms in water bodies, resulting in eutrophication. In this context, cultivation of photosynthetic microorganisms in secondary wastewater from sewage treatment allows the removal of nutrients from such wastewater, reducing the possibility of eutrophication. Moreover, microalgal biomass, produced in this tertiary wastewater treatment, may be harvested by different methods with the potential for different applications, such as fertilizer and biofuel.
A vinhaça é resultante da produção de álcool, após a fermentação do mosto e destilação do vinho. ... more A vinhaça é resultante da produção de álcool, após a fermentação do mosto e destilação do vinho. É um resíduo rico em nutrientes, principalmente matéria orgânica, nitrogênio, fósforo e potássio. Para ser despejado em rios e lagos, esse efluente deve passar por tratamentos para remoção desses nutrientes, pois o excesso desses elementos nos corpos hídricos poderia levar a grandes problemas ambientais, de modo que tem sido utilizado em fertirrigação. Microorganismos fotossintetizantes absorvem nutrientes inorgânicos, podendo absorver nutrientes de águas residuais. Se for removida a parte orgânica da vinhaça, a fração inorgânica ou com baixa carga orgânica pode ser a base ou o próprio meio de cultivo destes micro-organismos, que absorvem gás carbônico e sua biomassa é de interesse industrial. Neste contexto, foi estudado o cultivo de Monoraphidium contortum e Synechocystis salina, oriundos de água de mangue, em frascos de Erlenmeyer e em fotobiorreatores tubulares por processo descontín...
Abstract In this study, secondary effluent from dairy industry was employed for the cultivation o... more Abstract In this study, secondary effluent from dairy industry was employed for the cultivation of Chlorella vulgaris in a bench-scale tubular photobioreactor. We sought to evaluate the biomass production, the consumption of nitrogen in the medium, and the final biomass composition. Considering the need of nitrogen supplementation, we evaluated the nitrogen:phosphorus ratio: the same proportion found in Bold basal medium (N:P = 1.71:1) and Redfield ratio (N:P = 16:1), comparing with no nitrogen supplementation and Bold Basal Medium. The results showed that nitrogen supplementation improves biomass growth (up to 2824.93 mg.L-1), also granting efficient nutrients consumption (up to 98% of nitrogen removal). Nitrogen supplementation following the Bold medium ratio was the most suitable protocol, since it requires less nitrogen addition without impairment in biomass productivity (Px =259.90 mg.L-1.d-1), in comparison with cultures with supplementation following Redfield ratio (Px =296.73 mg.L-1.d-1) or control culture (Px =221.02 mg.L-1.d-1). In addition, the final biomass showed satisfactory amounts of proteins (up to 21.92 %) and lipids (up to 35.75 %), besides presenting a profile with high concentrations of saturated (C16:0) and monounsaturated fatty acids (C18:1).
Applied biochemistry and biotechnology, Jan 5, 2018
Since cultivations of Arthrospira platensis have a high water demand, it is necessary to develop ... more Since cultivations of Arthrospira platensis have a high water demand, it is necessary to develop treatment methods for reusing the exhausted medium that may prevent environmental problems and obtaining useful biomass. The exhausted Schlösser medium obtained from A. platensis batch cultivation in bench-scale mini-tanks was treated by varying concentrations of different coagulants, ferric chloride (6, 10, and 14 mg L) or ferric sulfate (15, 25, and 35 mg L) and powdered activated carbon (PAC, 30 and 50 mg L). Such treated effluent was restored with NaNOand reused in new cultivations of A. platensis performed in Erlenmeyer flasks. Reusing media through the cultivation of A. platensis showed satisfactory results, particularly in the medium treated with ferric chloride and PAC. The maximum cell concentration obtained in the flasks was 1093 mg L, which corresponded to the medium treated with ferric chloride (6 mg L) and PAC (30 mg L). This cellular growth was higher than in the medium tre...
MEJIA, L. C. Evaluation of the growth of photosynthetic microorganisms in tubular photobioreactor... more MEJIA, L. C. Evaluation of the growth of photosynthetic microorganisms in tubular photobioreactor using nutrients from treated vinasse. 2020. 92 p. (Doctoral Thesis) Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil. 2020. The vinasse is the result of the production of alcohol after the fermentation of the mash and the distillation of the wine. It is a waste rich in nutrients, mainly organic matter, nitrogen, phosphorus and potassium. To be discharged into rivers and lakes, this effluent must be treated in order to remove nutrients, because the excess of these elements in water bodies can lead to major environmental problems, so that it has been used in fertigation. Photosynthetic microorganisms absorb inorganic nutrients and they can absorb nutrients from wastewater. If the organic fraction of vinasse is removed, the inorganic or low organic fraction may be the basis or a medium of cultivation of these microorganisms, which absorb carbon dioxide and its...
Taking into account the worrying scenario of water scarcity, it is essential to enable more effic... more Taking into account the worrying scenario of water scarcity, it is essential to enable more efficient technologies for wastewater treatment. Wastewater may be treated by conventional biological processes that remove pathogenic organisms, particulate and soluble organic compounds, and other components. However, secondary effluents from treatment plants may still contain toxic elements or high concentrations of inorganic nutrients (mainly nitrogen and phosphorus), which enable the growth of photosynthetic microorganisms in water bodies, resulting in eutrophication. In this context, cultivation of photosynthetic microorganisms in secondary wastewater from sewage treatment allows the removal of nutrients from such wastewater, reducing the possibility of eutrophication. Moreover, microalgal biomass, produced in this tertiary wastewater treatment, may be harvested by different methods with the potential for different applications, such as fertilizer and biofuel.
A vinhaça é resultante da produção de álcool, após a fermentação do mosto e destilação do vinho. ... more A vinhaça é resultante da produção de álcool, após a fermentação do mosto e destilação do vinho. É um resíduo rico em nutrientes, principalmente matéria orgânica, nitrogênio, fósforo e potássio. Para ser despejado em rios e lagos, esse efluente deve passar por tratamentos para remoção desses nutrientes, pois o excesso desses elementos nos corpos hídricos poderia levar a grandes problemas ambientais, de modo que tem sido utilizado em fertirrigação. Microorganismos fotossintetizantes absorvem nutrientes inorgânicos, podendo absorver nutrientes de águas residuais. Se for removida a parte orgânica da vinhaça, a fração inorgânica ou com baixa carga orgânica pode ser a base ou o próprio meio de cultivo destes micro-organismos, que absorvem gás carbônico e sua biomassa é de interesse industrial. Neste contexto, foi estudado o cultivo de Monoraphidium contortum e Synechocystis salina, oriundos de água de mangue, em frascos de Erlenmeyer e em fotobiorreatores tubulares por processo descontín...
Abstract In this study, secondary effluent from dairy industry was employed for the cultivation o... more Abstract In this study, secondary effluent from dairy industry was employed for the cultivation of Chlorella vulgaris in a bench-scale tubular photobioreactor. We sought to evaluate the biomass production, the consumption of nitrogen in the medium, and the final biomass composition. Considering the need of nitrogen supplementation, we evaluated the nitrogen:phosphorus ratio: the same proportion found in Bold basal medium (N:P = 1.71:1) and Redfield ratio (N:P = 16:1), comparing with no nitrogen supplementation and Bold Basal Medium. The results showed that nitrogen supplementation improves biomass growth (up to 2824.93 mg.L-1), also granting efficient nutrients consumption (up to 98% of nitrogen removal). Nitrogen supplementation following the Bold medium ratio was the most suitable protocol, since it requires less nitrogen addition without impairment in biomass productivity (Px =259.90 mg.L-1.d-1), in comparison with cultures with supplementation following Redfield ratio (Px =296.73 mg.L-1.d-1) or control culture (Px =221.02 mg.L-1.d-1). In addition, the final biomass showed satisfactory amounts of proteins (up to 21.92 %) and lipids (up to 35.75 %), besides presenting a profile with high concentrations of saturated (C16:0) and monounsaturated fatty acids (C18:1).
Applied biochemistry and biotechnology, Jan 5, 2018
Since cultivations of Arthrospira platensis have a high water demand, it is necessary to develop ... more Since cultivations of Arthrospira platensis have a high water demand, it is necessary to develop treatment methods for reusing the exhausted medium that may prevent environmental problems and obtaining useful biomass. The exhausted Schlösser medium obtained from A. platensis batch cultivation in bench-scale mini-tanks was treated by varying concentrations of different coagulants, ferric chloride (6, 10, and 14 mg L) or ferric sulfate (15, 25, and 35 mg L) and powdered activated carbon (PAC, 30 and 50 mg L). Such treated effluent was restored with NaNOand reused in new cultivations of A. platensis performed in Erlenmeyer flasks. Reusing media through the cultivation of A. platensis showed satisfactory results, particularly in the medium treated with ferric chloride and PAC. The maximum cell concentration obtained in the flasks was 1093 mg L, which corresponded to the medium treated with ferric chloride (6 mg L) and PAC (30 mg L). This cellular growth was higher than in the medium tre...
MEJIA, L. C. Evaluation of the growth of photosynthetic microorganisms in tubular photobioreactor... more MEJIA, L. C. Evaluation of the growth of photosynthetic microorganisms in tubular photobioreactor using nutrients from treated vinasse. 2020. 92 p. (Doctoral Thesis) Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil. 2020. The vinasse is the result of the production of alcohol after the fermentation of the mash and the distillation of the wine. It is a waste rich in nutrients, mainly organic matter, nitrogen, phosphorus and potassium. To be discharged into rivers and lakes, this effluent must be treated in order to remove nutrients, because the excess of these elements in water bodies can lead to major environmental problems, so that it has been used in fertigation. Photosynthetic microorganisms absorb inorganic nutrients and they can absorb nutrients from wastewater. If the organic fraction of vinasse is removed, the inorganic or low organic fraction may be the basis or a medium of cultivation of these microorganisms, which absorb carbon dioxide and its...
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