Gating strategies to analyze lymphoid and myeloid subpopulations – To analyze T lymphocytes a tri... more Gating strategies to analyze lymphoid and myeloid subpopulations – To analyze T lymphocytes a triple staining strategy was used with CD5, CD4 and CD8 antibodies. First, all CD5+ cells were gated, and then, CD4 and CD8 expression was analyzed in this R1 population, characterizing CD5+CD4+ and CD5+CD8+ T lymphocytes (A). To quantify peritoneal B cells, a double staining strategy was used with B220 and CD11b antibodies, where B220+CD11b− cells were considered B2 lymphocytes and B220+CD11b+ cells were considered B1 lymphocytes (B). And, finally, to characterize myeloid cells, a triple staining strategy was used with CD11b, Gr-1 and Ly-6G antibodies. All CD11bhigh Gr-1high cells are neutrophils, as observed by Ly-6G expression, and cells which expressed low levels of Gr-1 (CD11bhigh Gr-1low cells) were considered myeloid progenitors (C). (PDF 53 kb)
In this paper, we introduce a geographic similarity operator that computes the relatedness betwee... more In this paper, we introduce a geographic similarity operator that computes the relatedness between two geographic places and describe how it is combined with textual ranking. The effectiveness of the geographic ranking is evaluated on the GeoCLEF 2005 collection. We considered various strategies for query formulation and for combining textual ant geographical ranking. For some queries, geographic ranking significantly improves results, while for other queries it does not have a positive impact.
2020 IEEE 32nd Conference on Software Engineering Education and Training (CSEE&T), 2020
Software Inspection is a review technique that aims to improve the quality of software artifacts ... more Software Inspection is a review technique that aims to improve the quality of software artifacts through defect detection, thus reducing the cost of rework. Being a human-driven process, it depends directly on the professional's individual expertise level in the field. However, it is challenging to teach software inspection in the classroom without proper support, leading students to lose motivation and hampering their learning. To circumvent that, the InspectorX serious game was proposed as a means to support Software Inspection Education and Training. Since then, students provided feedback on what could make it more interesting. In this sense, this paper introduces InspectorX 2.0, an evolution that migrate old issues and present novel features. A preliminary assessment performed with students helped us understand the pros and cons of this version.
O proposito do artigo e analisar as condicoes sociais e economicas da populacao da Regiao Adminis... more O proposito do artigo e analisar as condicoes sociais e economicas da populacao da Regiao Administrativa de Franca - SP, sintetizadas atraves do Indice de Exclusao Social (IES). O desenvolvimento, como um sistema, esta conectado a outros subsistemas: o politico, o economico, o social e o ambiental, o que nos leva a compreende-lo, dada sua interdependencia, complexidade e abrangencia, senao por meio de novas abordagens. Por esta razao, o artigo assenta-se na visao sistemica e na abordagem interdisciplinar. Trata-se de uma pesquisa bibliografica acrescida de uma pesquisa quantitativa baseada em indicadores sociais e economicos. O resultado da pesquisa revelou-nos que nos municipios que integram a Regiao Administrativa de Franca, os indicadores apontam boas condicoes sociais da populacao, contudo avancos em algumas dimensoes sao ofuscados por retrocessos em outras. Ainda que, inconclusiva e provisoriamente, acreditamos que em alguma medida, muito ainda precisamos avancar no campo da in...
Anais do 15. Congresso Brasileiro de Inteligência Computacional, 2021
A SEFAZ-RJ possui um canal de atendimento pelo Sistema “Fale Conosco” usado para esclarecer dúvid... more A SEFAZ-RJ possui um canal de atendimento pelo Sistema “Fale Conosco” usado para esclarecer dúvidas de contribuintes enviadas por e-mail. Com o distanciamento social, ocasionado pela COVID-19, a automatização das respostas para um maior volume de mensagens recebidas, tornou-se fundamental. Dessa forma, esse trabalho apresenta investigação de técnicas de Mineração de Texto, visando, em um primeiro momento, a classificação das mensagens de contribuintes a partir de técnicas de Machine Learning/Deep Learning para a automatização do processo de respostas aos contribuintes. Os resultados obtidos contribuíram para uma proposta de reformulação do formulário de dúvidas dos contribuintes, além de indicarem técnicas mais promissoras para se iniciar o processo de automatização do ``Fale Conosco'' da SEFAZ-RJ.
Investigations using preclinical models of preterm birth have much contributed, together with hum... more Investigations using preclinical models of preterm birth have much contributed, together with human neuropathological studies, for advances in our understanding of preterm brain injury. Here, we evaluated whether the neurodevelopmental and behavioral consequences of preterm birth induced by a non-inflammatory model of preterm birth using mifepristone would differ from those after inflammatory prenatal transient hypoxia-ischemia (TSHI) model. Pregnant Wistar rats were either injected with mifepristone, and pups were delivered on embryonic day 21 (ED21 group), or laparotomized on the 18th day of gestation for 60 minutes of uterine arteries occlusion. Rat pups were tested postnatally for characterization of developmental milestones and, after weaning, they were behaviorally tested for anxiety and for spatial learning and memory. One month later, brains were processed for quantification of doublecortin (DCX)- and neuropeptide Y (NPY)-immunoreactive cells, and cholinergic varicosities in the hippocampus. ED21 rats did not differ from controls with respect to neonatal developmental milestones, anxiety, learning and memory functions, and neurochemical parameters. Conversely, in TSHI rats the development of neonatal reflexes was delayed, the levels of anxiety were reduced, and spatial learning and memory was impaired; in the hippocampus, the total number of DCX and NPY cells was increased, and the density of cholinergic varicosities was reduced. With these results we suggest that a preterm birth, in a non-inflammatory prenatal environment, does not significantly change neonatal development and adult neurologic outcome. On other hand, prenatal hypoxia and ischemia (inflammation) modifies developmental trajectory, learning and memory, neurogenesis, and NPY GABAergic and cholinergic brain systems.
Sobre desertos, transmutações, ditirambos e olhares. Um encontro. Duas pessoas. Universos. Compon... more Sobre desertos, transmutações, ditirambos e olhares. Um encontro. Duas pessoas. Universos. Compondo em meio a fragmentos, criando uma singularidade.
Na América Latina, a escalada da violência, notadamente a taxa de homicídios entre jovens, é sufi... more Na América Latina, a escalada da violência, notadamente a taxa de homicídios entre jovens, é suficientemente elevada para classifica-la como epidêmica. No Brasil, a situação não é tão diferente, entretanto, o fenômeno que outrora era mais pronunciado nas áreas metropolitanas, deslocou-se para o interior, redesenhando desta maneira, uma nova geografia da violência. Além disto, a pesquisa baseou-se em indicadores demográficos e sociais cujo propósito foi verificar se existe alguma relação causal deles com este fenômeno. A pesquisa conclui que bons indicadores sociais podem contribuir positivamente para a redução dos homicídios, contudo não são suficientes para explicar suas causas.
To analyze the level of apoptosis in different mucosal compartments and the differential expressi... more To analyze the level of apoptosis in different mucosal compartments and the differential expression of Fas/Fas-ligand and perforin in H pylori-associated gastric ulcer. Antral specimens from patients with H pylori-related active gastric ulcer (GU), H pylori-related gastritis, and non-infected controls were analysed for densities and distribution of apoptotic cells determined by the TdT-mediated dUDP-biotin nick-end-labelling method. GU patients were submitted to eradication therapy with follow-up biopsy after 60 d. Fas, FasL, and perforin-expressing cells were assessed by immunoperoxidase, and with anti-CD3, anti-CD20 and anti-CD68 by double immunofluorescence and confocal microscopy. Quantitative analysis was performed using a computer-assisted image analyser. H pylori-infected antrum showed greater surface epithelial apoptosis which decreased after eradication therapy. In the lamina propria, higher rates of mononuclear cell apoptosis were observed in H pylori-gastritis. Co-expression of Fas with T-cell and macrophage markers was reduced in GU. FasL- and perforin-expressing cells were increased in H pylori-infection and correlated with epithelial apoptosis. Perforin-expressing cells were also increased in GU compared with H pylori-gastritis. Epithelial apoptosis is increased in H pylori-infection and correlates to FasL- and perforin-expression by T cells. Expression of perforin is correlated with the tissue damage, and may represent the enhancement of a distinct cytotoxic pathway in GU. Increased expression of FasL not paralleled by Fas on T-cells and macrophages may indicate a reduced susceptibility to the Fas/FasL-mediated apoptosis of lymphoid cells in H pylori-infection.
Extended-spectrum beta-lactamase-producing Escherichia coli isolated from poultry in Brazil showe... more Extended-spectrum beta-lactamase-producing Escherichia coli isolated from poultry in Brazil showed bla(CTX-M-8) gene. IS10 was found upstream of bla(CTX-M-8), harbored on plasmids Inch, ST113/ST114 subtypes. Genomic relationship revealed a heterogeneous E. coli population. The gene bla(CTX-M-8) is established in South America in food-producing animals, which represent risk of dissemination for other countries.
Ninth IEEE International Symposium on Distributed Simulation and Real-Time Applications
A Novel Solution for the Development of Collaborative Virtual Environment Simulations in Large Sc... more A Novel Solution for the Development of Collaborative Virtual Environment Simulations in Large Scale Azzedine Boukerche 1 , Diego Duarte 2 , Regina Araujo 2 , Leonardo Andrade 2 , Anis Zarrad 1 1 SITE University of Ottawa Ottawa, Canada boukerch@site.uottawa.ca ...
Background: The pathological differential diagnosis between primary and metastatic ovarian malign... more Background: The pathological differential diagnosis between primary and metastatic ovarian malignant tumors is usually difficult, specially for tumors originating in the gastrointestinal system or breast. Aim: To define an immunohistochemical flow chart to discriminate these tumor types. Material and methods: We performed a immunostaining analysis using a panel of six antibodies (CK 7, CA 125, CEA, CK20, BRST-2 and CA 15-3) in 3 tumor groups: 11 ovarian, 14 breast and 12 colonic primary tumors and in a study group of 38 ovarian tumors whose primary origin was unknown. Results: We defined an ovarian immunohistochemical pattern (CEA-/CK20-, 45% in ovarian tumors, 0% in breast or colonic tumors, p <0.001); an ovary/breast pattern (CEA+/CK20-, 0% in colonic tumors, p <0.001): a breast pattern (CEA+/CK20-/BKST-2+, 64% in breast tumors and 0% in colonic and ovarian tumors, p <0.001) and a colonic pattern (CEA+/ CK20+/CK7-, 67% in colonic tumors and 0% in breast and ovarian tumors, p <0.001). Conclusions: Employing a simple panel of antibodies, characteristic immunohistochemical patterns were defined for ovarian, breast and colonic tumors. These patterns allowed the identification of the origin of most tumors of the study group (Rev Med Chile 2002; 130: 1232-40)
Dois experimentos foram conduzidos para avaliar o desempenho, o balanço e a retenção de MS, EE e ... more Dois experimentos foram conduzidos para avaliar o desempenho, o balanço e a retenção de MS, EE e PB e os parâmetros biométricos do trato gastrintestinal de frangos de corte recebendo diferentes níveis de proteína na ração pré-inicial (1 a 7 dias). No experimento 1, 560 pintos Avian Farms foram distribuídos em baterias segundo um delineamento inteiramente casualizado, em esquema fatorial 5 x 2 (cinco níveis de proteína, 18, 20, 22, 24 e 26%, e dois sexos), com dez tratamentos, cada um com quatro repetições de 14 aves. No experimento 2, 1.860 pintos machos e fêmeas Ross foram distribuídos em boxes de 2,0 x 2,5 m, em um delineamento em blocos casualizados, com cinco tratamentos (níveis de proteína, 18, 20, 22, 24 e 26%), cada um com seis repetições de 62 aves. No experimento 1, os níveis crescentes de PB da dieta não afetaram significativamente os dados de desempenho, mas o balanço e a quantidade retida do EE promoveram efeito linear positivo. O balanço de proteína não foi afetado, mas...
Gating strategies to analyze lymphoid and myeloid subpopulations – To analyze T lymphocytes a tri... more Gating strategies to analyze lymphoid and myeloid subpopulations – To analyze T lymphocytes a triple staining strategy was used with CD5, CD4 and CD8 antibodies. First, all CD5+ cells were gated, and then, CD4 and CD8 expression was analyzed in this R1 population, characterizing CD5+CD4+ and CD5+CD8+ T lymphocytes (A). To quantify peritoneal B cells, a double staining strategy was used with B220 and CD11b antibodies, where B220+CD11b− cells were considered B2 lymphocytes and B220+CD11b+ cells were considered B1 lymphocytes (B). And, finally, to characterize myeloid cells, a triple staining strategy was used with CD11b, Gr-1 and Ly-6G antibodies. All CD11bhigh Gr-1high cells are neutrophils, as observed by Ly-6G expression, and cells which expressed low levels of Gr-1 (CD11bhigh Gr-1low cells) were considered myeloid progenitors (C). (PDF 53 kb)
In this paper, we introduce a geographic similarity operator that computes the relatedness betwee... more In this paper, we introduce a geographic similarity operator that computes the relatedness between two geographic places and describe how it is combined with textual ranking. The effectiveness of the geographic ranking is evaluated on the GeoCLEF 2005 collection. We considered various strategies for query formulation and for combining textual ant geographical ranking. For some queries, geographic ranking significantly improves results, while for other queries it does not have a positive impact.
2020 IEEE 32nd Conference on Software Engineering Education and Training (CSEE&T), 2020
Software Inspection is a review technique that aims to improve the quality of software artifacts ... more Software Inspection is a review technique that aims to improve the quality of software artifacts through defect detection, thus reducing the cost of rework. Being a human-driven process, it depends directly on the professional's individual expertise level in the field. However, it is challenging to teach software inspection in the classroom without proper support, leading students to lose motivation and hampering their learning. To circumvent that, the InspectorX serious game was proposed as a means to support Software Inspection Education and Training. Since then, students provided feedback on what could make it more interesting. In this sense, this paper introduces InspectorX 2.0, an evolution that migrate old issues and present novel features. A preliminary assessment performed with students helped us understand the pros and cons of this version.
O proposito do artigo e analisar as condicoes sociais e economicas da populacao da Regiao Adminis... more O proposito do artigo e analisar as condicoes sociais e economicas da populacao da Regiao Administrativa de Franca - SP, sintetizadas atraves do Indice de Exclusao Social (IES). O desenvolvimento, como um sistema, esta conectado a outros subsistemas: o politico, o economico, o social e o ambiental, o que nos leva a compreende-lo, dada sua interdependencia, complexidade e abrangencia, senao por meio de novas abordagens. Por esta razao, o artigo assenta-se na visao sistemica e na abordagem interdisciplinar. Trata-se de uma pesquisa bibliografica acrescida de uma pesquisa quantitativa baseada em indicadores sociais e economicos. O resultado da pesquisa revelou-nos que nos municipios que integram a Regiao Administrativa de Franca, os indicadores apontam boas condicoes sociais da populacao, contudo avancos em algumas dimensoes sao ofuscados por retrocessos em outras. Ainda que, inconclusiva e provisoriamente, acreditamos que em alguma medida, muito ainda precisamos avancar no campo da in...
Anais do 15. Congresso Brasileiro de Inteligência Computacional, 2021
A SEFAZ-RJ possui um canal de atendimento pelo Sistema “Fale Conosco” usado para esclarecer dúvid... more A SEFAZ-RJ possui um canal de atendimento pelo Sistema “Fale Conosco” usado para esclarecer dúvidas de contribuintes enviadas por e-mail. Com o distanciamento social, ocasionado pela COVID-19, a automatização das respostas para um maior volume de mensagens recebidas, tornou-se fundamental. Dessa forma, esse trabalho apresenta investigação de técnicas de Mineração de Texto, visando, em um primeiro momento, a classificação das mensagens de contribuintes a partir de técnicas de Machine Learning/Deep Learning para a automatização do processo de respostas aos contribuintes. Os resultados obtidos contribuíram para uma proposta de reformulação do formulário de dúvidas dos contribuintes, além de indicarem técnicas mais promissoras para se iniciar o processo de automatização do ``Fale Conosco'' da SEFAZ-RJ.
Investigations using preclinical models of preterm birth have much contributed, together with hum... more Investigations using preclinical models of preterm birth have much contributed, together with human neuropathological studies, for advances in our understanding of preterm brain injury. Here, we evaluated whether the neurodevelopmental and behavioral consequences of preterm birth induced by a non-inflammatory model of preterm birth using mifepristone would differ from those after inflammatory prenatal transient hypoxia-ischemia (TSHI) model. Pregnant Wistar rats were either injected with mifepristone, and pups were delivered on embryonic day 21 (ED21 group), or laparotomized on the 18th day of gestation for 60 minutes of uterine arteries occlusion. Rat pups were tested postnatally for characterization of developmental milestones and, after weaning, they were behaviorally tested for anxiety and for spatial learning and memory. One month later, brains were processed for quantification of doublecortin (DCX)- and neuropeptide Y (NPY)-immunoreactive cells, and cholinergic varicosities in the hippocampus. ED21 rats did not differ from controls with respect to neonatal developmental milestones, anxiety, learning and memory functions, and neurochemical parameters. Conversely, in TSHI rats the development of neonatal reflexes was delayed, the levels of anxiety were reduced, and spatial learning and memory was impaired; in the hippocampus, the total number of DCX and NPY cells was increased, and the density of cholinergic varicosities was reduced. With these results we suggest that a preterm birth, in a non-inflammatory prenatal environment, does not significantly change neonatal development and adult neurologic outcome. On other hand, prenatal hypoxia and ischemia (inflammation) modifies developmental trajectory, learning and memory, neurogenesis, and NPY GABAergic and cholinergic brain systems.
Sobre desertos, transmutações, ditirambos e olhares. Um encontro. Duas pessoas. Universos. Compon... more Sobre desertos, transmutações, ditirambos e olhares. Um encontro. Duas pessoas. Universos. Compondo em meio a fragmentos, criando uma singularidade.
Na América Latina, a escalada da violência, notadamente a taxa de homicídios entre jovens, é sufi... more Na América Latina, a escalada da violência, notadamente a taxa de homicídios entre jovens, é suficientemente elevada para classifica-la como epidêmica. No Brasil, a situação não é tão diferente, entretanto, o fenômeno que outrora era mais pronunciado nas áreas metropolitanas, deslocou-se para o interior, redesenhando desta maneira, uma nova geografia da violência. Além disto, a pesquisa baseou-se em indicadores demográficos e sociais cujo propósito foi verificar se existe alguma relação causal deles com este fenômeno. A pesquisa conclui que bons indicadores sociais podem contribuir positivamente para a redução dos homicídios, contudo não são suficientes para explicar suas causas.
To analyze the level of apoptosis in different mucosal compartments and the differential expressi... more To analyze the level of apoptosis in different mucosal compartments and the differential expression of Fas/Fas-ligand and perforin in H pylori-associated gastric ulcer. Antral specimens from patients with H pylori-related active gastric ulcer (GU), H pylori-related gastritis, and non-infected controls were analysed for densities and distribution of apoptotic cells determined by the TdT-mediated dUDP-biotin nick-end-labelling method. GU patients were submitted to eradication therapy with follow-up biopsy after 60 d. Fas, FasL, and perforin-expressing cells were assessed by immunoperoxidase, and with anti-CD3, anti-CD20 and anti-CD68 by double immunofluorescence and confocal microscopy. Quantitative analysis was performed using a computer-assisted image analyser. H pylori-infected antrum showed greater surface epithelial apoptosis which decreased after eradication therapy. In the lamina propria, higher rates of mononuclear cell apoptosis were observed in H pylori-gastritis. Co-expression of Fas with T-cell and macrophage markers was reduced in GU. FasL- and perforin-expressing cells were increased in H pylori-infection and correlated with epithelial apoptosis. Perforin-expressing cells were also increased in GU compared with H pylori-gastritis. Epithelial apoptosis is increased in H pylori-infection and correlates to FasL- and perforin-expression by T cells. Expression of perforin is correlated with the tissue damage, and may represent the enhancement of a distinct cytotoxic pathway in GU. Increased expression of FasL not paralleled by Fas on T-cells and macrophages may indicate a reduced susceptibility to the Fas/FasL-mediated apoptosis of lymphoid cells in H pylori-infection.
Extended-spectrum beta-lactamase-producing Escherichia coli isolated from poultry in Brazil showe... more Extended-spectrum beta-lactamase-producing Escherichia coli isolated from poultry in Brazil showed bla(CTX-M-8) gene. IS10 was found upstream of bla(CTX-M-8), harbored on plasmids Inch, ST113/ST114 subtypes. Genomic relationship revealed a heterogeneous E. coli population. The gene bla(CTX-M-8) is established in South America in food-producing animals, which represent risk of dissemination for other countries.
Ninth IEEE International Symposium on Distributed Simulation and Real-Time Applications
A Novel Solution for the Development of Collaborative Virtual Environment Simulations in Large Sc... more A Novel Solution for the Development of Collaborative Virtual Environment Simulations in Large Scale Azzedine Boukerche 1 , Diego Duarte 2 , Regina Araujo 2 , Leonardo Andrade 2 , Anis Zarrad 1 1 SITE University of Ottawa Ottawa, Canada boukerch@site.uottawa.ca ...
Background: The pathological differential diagnosis between primary and metastatic ovarian malign... more Background: The pathological differential diagnosis between primary and metastatic ovarian malignant tumors is usually difficult, specially for tumors originating in the gastrointestinal system or breast. Aim: To define an immunohistochemical flow chart to discriminate these tumor types. Material and methods: We performed a immunostaining analysis using a panel of six antibodies (CK 7, CA 125, CEA, CK20, BRST-2 and CA 15-3) in 3 tumor groups: 11 ovarian, 14 breast and 12 colonic primary tumors and in a study group of 38 ovarian tumors whose primary origin was unknown. Results: We defined an ovarian immunohistochemical pattern (CEA-/CK20-, 45% in ovarian tumors, 0% in breast or colonic tumors, p <0.001); an ovary/breast pattern (CEA+/CK20-, 0% in colonic tumors, p <0.001): a breast pattern (CEA+/CK20-/BKST-2+, 64% in breast tumors and 0% in colonic and ovarian tumors, p <0.001) and a colonic pattern (CEA+/ CK20+/CK7-, 67% in colonic tumors and 0% in breast and ovarian tumors, p <0.001). Conclusions: Employing a simple panel of antibodies, characteristic immunohistochemical patterns were defined for ovarian, breast and colonic tumors. These patterns allowed the identification of the origin of most tumors of the study group (Rev Med Chile 2002; 130: 1232-40)
Dois experimentos foram conduzidos para avaliar o desempenho, o balanço e a retenção de MS, EE e ... more Dois experimentos foram conduzidos para avaliar o desempenho, o balanço e a retenção de MS, EE e PB e os parâmetros biométricos do trato gastrintestinal de frangos de corte recebendo diferentes níveis de proteína na ração pré-inicial (1 a 7 dias). No experimento 1, 560 pintos Avian Farms foram distribuídos em baterias segundo um delineamento inteiramente casualizado, em esquema fatorial 5 x 2 (cinco níveis de proteína, 18, 20, 22, 24 e 26%, e dois sexos), com dez tratamentos, cada um com quatro repetições de 14 aves. No experimento 2, 1.860 pintos machos e fêmeas Ross foram distribuídos em boxes de 2,0 x 2,5 m, em um delineamento em blocos casualizados, com cinco tratamentos (níveis de proteína, 18, 20, 22, 24 e 26%), cada um com seis repetições de 62 aves. No experimento 1, os níveis crescentes de PB da dieta não afetaram significativamente os dados de desempenho, mas o balanço e a quantidade retida do EE promoveram efeito linear positivo. O balanço de proteína não foi afetado, mas...
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