By performing a high-resolution spatial-genetic analysis of a partially clonal Salvia brachyodon ... more By performing a high-resolution spatial-genetic analysis of a partially clonal Salvia brachyodon population, we elucidated its clonal architecture and seedling recruitment strategy. The sampling of the entire population was based on a 1 × 1 m grid and each sampled individual was genotyped. Population-genetic statistics were combined with geospatial analyses. On the population level, the presence of both sexual and clonal reproduction and repeated seedling recruitment as the prevailing strategy of new genets establishment were confirmed. On the patch level, a phalanx clonal architecture was detected. A significant negative correlation between patches’ sizes and genotypic richness was observed as young plants were not identified within existing patches of large genets but almost exclusively in surrounding areas. The erosion of the genetic variability of older patches is likely caused by the inter-genet competition and resulting selection or by a random die-off of individual genets acc...
Leaves play a central role in plant fitness, allowing efficient light capture, gas exchange and t... more Leaves play a central role in plant fitness, allowing efficient light capture, gas exchange and thermoregulation, ensuring optimal growing conditions for the plant. Phenotypic variability in leaf shape and size has been linked to environmental heterogeneity and habitat characteristics. Therefore, the study of foliar morphology in plant populations can help us to identify the environmental factors that may have influenced the process of species diversification. In this study, we used European wild pear (Pyrus pyraster (L.) Burgsd., Rosaceae) as a model species to investigate the phenotypic variability of leaves under different environmental conditions. Using leaf morphometric data from 19 natural populations from the north-western part of the Balkan Peninsula, a high level of variability among and within populations were found. Leaf traits related to leaf size were more variable compared to leaf shape traits, with both influenced by geographic and environmental factors. Consequently,...
With the development of next-generation sequencing technology and bioinformatics tools, the proce... more With the development of next-generation sequencing technology and bioinformatics tools, the process of assembling DNA sequences has become cheaper and easier, especially in the case of much shorter organelle genomes. The number of available DNA sequences of complete chloroplast genomes in public genetic databases is constantly increasing and the data are widely used in plant phylogenetic and biotechnological research. In this work, we investigated possible inconsistencies in the stored form of publicly available chloroplast genome sequence data. The impact of these inconsistencies on the results of the phylogenetic analysis was investigated and the bioinformatic solution to identify and correct inconsistencies was implemented. The whole procedure was demonstrated using five plant families (Apiaceae, Asteraceae, Campanulaceae, Lamiaceae and Rosaceae) as examples.
Landraces represent valuable genetic resources for breeding programmes to produce high-yielding v... more Landraces represent valuable genetic resources for breeding programmes to produce high-yielding varieties adapted to stressful environmental conditions. Although the common bean (Phaseolus vulgaris L.) is an economically important food legume for direct human consumption worldwide, common bean production in Croatia is based almost exclusively on landraces and there is no common bean breeding program. Information on phaseolin type and results of population structure and genetic diversity obtained by analysis of SSR and SNP markers, in combination with the morphological characterization of 174 accessions of 10 common bean landraces (morphotypes), enabled thorough classification of accessions. The accessions were classified into phaseolin type H1 (“S”) of Mesoamerican origin and phaseolin types H2 (“H” or “C”) and H3 (“T”) of Andean origin. By applying distance- and model-based clustering methods to SSR markers, the accessions were classified into two clusters at K = 2 separating the a...
Ramanova spektroskopija je vibracijska spektroskopska metoda koja omogucava analizu spektra razli... more Ramanova spektroskopija je vibracijska spektroskopska metoda koja omogucava analizu spektra razlicitih materijala, ukljucujuci i sekundarne biljne metabolite, ovisno o molekularnoj strukturi i vrsti kemijskih veza. Ova je tehnika prikladna za utvrđivanje i lokalizaciju razlicitih bioaktivnih spojeva kao sto su terpeni, alkaloidi, fenolni spojevi i dr. Kod dalmatinskog buhaca (Tanacetum cinerariifolium /Trevir./ Sch. Bip. ; Asteraceae) aktivna insekticidna tvar je piretrin kojeg cini sest sastavnica (piretrin I i II, cinerin I i II te jasmolin I i II). Utvrđivanje nazocnosti piretrina u ekstraktima cvjetnih glavica dalmatinskog buhaca provodeno je pomocu Ramanove spektroskopije. Prosjecne vrijednosti Ramanovih spektara analiziranih uzoraka ukazuju na nazocnost karotenoida u Ramanovom opsegu od 1000 do 1530 cm-1. Karakteristicni signali visokog intenziteta koji ukazuju na nazocnost piretrina I i II uoceni su u Ramanovim spektrima svih uzoraka na pozicijama 1438, 1600 i 1634 cm-1, dok ...
This study, conducted in Croatia, encompassed for the first time ever populations of the field el... more This study, conducted in Croatia, encompassed for the first time ever populations of the field elm that are solely natural. We studied the genetic diversity and assessed recent bottleneck events and the population structure of 5 natural field elm populations from continental and Mediterranean Croatia, using 5 microsatellite markers. The study results suggest that the observed populations are characterized by a satisfactory amount of heterozygosity, and that the impact of the Dutch elm disease on the amount of genetic diversity in sampled populations is currently negligible. As genetic diversity in forest tree species is slowly diminishing, in the long term there is a danger of the depletion of genetic resources. The danger of inbreeding within populations is low, and the majority of the populations are in Hardy-Weinberg equilibrium. The appearance of bottleneck, initiated by anthropogenic habitat destruction, has been established in only one population. The values of Nei’s standard ...
The systematic status of Dalmatian black pine is still unexplained. This black pine is usually co... more The systematic status of Dalmatian black pine is still unexplained. This black pine is usually considered as subspecies but sometimes also as a separate species. Austrian black pine from Botanical Garden Zagreb, Dalmatian black pine from the mount of Biokovo and Dalmatian black pine from the island of Hvar were used in this study. Two methods of plant molecular systematics have been applied in this work: restriction fragment length polymorphisms of chloroplast DNA (RFLP cpDNA) and random amplified polymorphic DNA (RAPD). The results of RFLP analysis of chloroplast DNA have been showed that there is no differences in restriction fragments among all investigated plants. The RAPD method which is the most appropriate for plant systematic at the species level and below, has been shown DNA polymorphisms between and within investigated populations, distinctly separated Austrian and Dalmatian black pine. The subspecies level for Dalmatian black pine has been strongly supported by these meth...
Jos u davnoj proslosti covjeku su sume pitomoga kestena predstavljale važan izvor razlicitih siro... more Jos u davnoj proslosti covjeku su sume pitomoga kestena predstavljale važan izvor razlicitih sirovina. Intenzivnim koristenjem kestenovih suma i nasada te pojavom raka kestenove kore, sredinom proslog stoljeca doslo je do njihovog propadanja i susenja. Iz tih je razloga u mnogim europskim zemljama pokrenut niz multidisciplinarnih projekata kojima je svrha ocuvanje genskih resursa pitomoga kestena i njegovog povrataka na nekadasnje znacajno mjesto u sumskim ekosustavima. Cilj ovog rada bio je utvrditi geneticku raznolikost populacija europskoga pitomoga kestena u sredisnjoj Europi i zapadnom dijelu Balkanskoga poluotoka. Ukupno je sakupljen 301 uzorak iz 15 populacija, a analiza geneticke raznolikosti provedena je pomocu deset mikrosatelitnih biljega. Ukupno je umnoženo 125 alela, s prosjecnom vrijednosti od 12, 5 alela po lokusu. Aleleno bogatstvo se kretalo od 4, 0 do 6, 1, s prosjecnom vrijednosti od 4, 9. Ni u jednoj populaciji nije utvrđeno statisticki znacajno odstupanje od Har...
The long tradition of green bean (Phaseolus vulgaris L.) cultivation in Croatia has resulted in t... more The long tradition of green bean (Phaseolus vulgaris L.) cultivation in Croatia has resulted in the development of a landraces adapted to specific areas and chosen to suit different dietary habits. The hypothesis of our study was that nutritional quality (content of proteins, sugars, fibres, calcium, potassium and phosphorus in fresh pods) of 14 widely grown Croatian green bean accessions is affected by origin and morphotype. The analysed accessions were grown in non-replicated field plots where morphotypes were classified. The gene pool of adscription (Andean vs. Mesoamerican) of each accession was determined with the phaseolin marker, whereas nutritional quality traits were analysed according to standard analytical methods. The relationships among nutritional quality traits of fresh pods were assessed by Pearson’s correlation coefficient. The univariate ANOVA and a post hoc Tukey test (p0.05) were carried out for each trait to compare the values among accessions. Accessions were ...
Summary Dalmatian or common sage (Salvia offi cinalis L.) is an outcrossing plant species native ... more Summary Dalmatian or common sage (Salvia offi cinalis L.) is an outcrossing plant species native to East Adriatic coast. Random Amplifi ed Polymorphic DNA markers (RAPD) were used to analyze genetic diversity and structure of ten natural populations from the East-Adriatic coastal region. Th e highest genetic diversity was found in populations from the central and south Dalmatia, while the highest frequency down-weighted marker values were found in the northernmost populations and the southern most inland population. Although analysis of molecular variance (AMOVA) revealed that most of the genetic diversity was attributable to diff erences among individuals within populations, highly signifi cant ST values suggested the existence of genetic diff erentiation among populations. By assuming Hardy-Weinberg equilibrium within populations, the calculated FST value among population was moderate. Bayesian model-based clustering method revealed that at K = 2 all individuals belonging to two ...
Dalmatian sage (Salvia officinalis L.) and Greek sage (S. fruticosa Mill.) are two closely relate... more Dalmatian sage (Salvia officinalis L.) and Greek sage (S. fruticosa Mill.) are two closely related species. While Dalmatian sage is naturally widespread on the Apennines and eastern Adriatic coast, Greek sage is naturally widespread in the eastern Mediterranean. The island of Vis is the only place in Croatia where S. fruticosa has been noticed. The presence of this species in Croatia, far away from its natural range, may be related to ancient Greek colonisation of the island in the fourth century BCE. From that time Greek sage probably escaped from gardens and vineyards and successfully naturalised western part of the island. Besides sharing the same habitat on the island of Vis, Dalmatian and Greek sage have the same chromosome number (2n = 14) and their flowering times overlap. The fact that the artificial hybrids between these two species are known and commercially exploited encouraged us to study spontaneous hybridization on the island of Vis. A total of 79 plant samples represe...
Summary Dalmatian sage (Salvia offi cinalis L.) represents one of the most signifi cant medicinal... more Summary Dalmatian sage (Salvia offi cinalis L.) represents one of the most signifi cant medicinal autochthonous species in fl ora of eastern Adriatic coast and islands. It is evergreen outcrossing perennial subshrub with short woody stems that branch extensively and violet fl owers. Apart from being native to Mediterranean karst of west Balkan and Apenine peninsula it is cultivated in numerous countries worldwide with Mediterranean and temperate continental climate. From the earliest times it has been used in traditional medicine in healing gingiva, mouth cavity and the sore throat, against bacterial and fungal infections, for wound treatment, memory enhancement, for treating common cold, against sweating, stomach infl ammation, ulcer formation, etc. Its essential oil has also been used in preservation of food and as spice as it gives both specifi c aroma and promotes digestion of food. Th e essential oil is extremely complex mixture of diff erent active ingredients; however, the th...
Almond-leaved pear (Pyrus spinosa Forssk., Rosaceae) is a scientifically poorly researched and of... more Almond-leaved pear (Pyrus spinosa Forssk., Rosaceae) is a scientifically poorly researched and often overlooked Mediterranean species. It is an insect-pollinated and animal-dispersed spiny, deciduous shrub or a small tree, with high-quality wood and edible fruits. The aim of the study was to assess the phenotypic diversity of almond-leaved pear in the eastern Adriatic region. The examination of phenotypic diversity was based on a morphometric analysis of 17 populations using ten phenotypic traits of leaves. Varieties of multivariate statistical analyses were conducted to evaluate the within- and among-population diversity. In addition, the Mantel tests were used to test the correlations between geographic, environmental, and phenotypic differences among populations. High phenotypic variability was determined both among and within the studied populations. Leaf-size-related traits proved to be the most variable ones, in contrast to more uniform leaf shape traits. Furthermore, three gr...
Short-tooth sage (Salvia brachyodon Vandas) is an endemic and one of the rarest plant species of ... more Short-tooth sage (Salvia brachyodon Vandas) is an endemic and one of the rarest plant species of the Dinaric karst. Although older literature indicates its presence in more localities; only two have been confirmed at the present time; Mt. Orjen (border of Bosnia and Herzegovina and Montenegro) and Peljesac peninsula (Croatia). Due to its very narrow distribution and economically very interesting essential oil composition short-tooth sage might become more endangered in the future. The aim of this research was to determine and compare inter-and intra- population morphological diversity of the only two noted natural populations of short-tooth sage by analyzing 16 quantitative morphological traits. Peljesac population of short-tooth sage showed considerably higher values for traits related to habitus and plant height; while the Mt. Orjen population showed higher values for traits related to the calyx and higher occurrence of sessile glandular hairs on the flower pedicels. When interpre...
Sa??etak: Croatian olive cultivar Lastovka is mainly used for oil production, Its fruity yield is... more Sa??etak: Croatian olive cultivar Lastovka is mainly used for oil production, Its fruity yield is high and regular but due to sensitivity to low temperatures its growing area is limited, with production concentrated on the island of Korcula. Different morphological types have ...
By performing a high-resolution spatial-genetic analysis of a partially clonal Salvia brachyodon ... more By performing a high-resolution spatial-genetic analysis of a partially clonal Salvia brachyodon population, we elucidated its clonal architecture and seedling recruitment strategy. The sampling of the entire population was based on a 1 × 1 m grid and each sampled individual was genotyped. Population-genetic statistics were combined with geospatial analyses. On the population level, the presence of both sexual and clonal reproduction and repeated seedling recruitment as the prevailing strategy of new genets establishment were confirmed. On the patch level, a phalanx clonal architecture was detected. A significant negative correlation between patches’ sizes and genotypic richness was observed as young plants were not identified within existing patches of large genets but almost exclusively in surrounding areas. The erosion of the genetic variability of older patches is likely caused by the inter-genet competition and resulting selection or by a random die-off of individual genets acc...
Leaves play a central role in plant fitness, allowing efficient light capture, gas exchange and t... more Leaves play a central role in plant fitness, allowing efficient light capture, gas exchange and thermoregulation, ensuring optimal growing conditions for the plant. Phenotypic variability in leaf shape and size has been linked to environmental heterogeneity and habitat characteristics. Therefore, the study of foliar morphology in plant populations can help us to identify the environmental factors that may have influenced the process of species diversification. In this study, we used European wild pear (Pyrus pyraster (L.) Burgsd., Rosaceae) as a model species to investigate the phenotypic variability of leaves under different environmental conditions. Using leaf morphometric data from 19 natural populations from the north-western part of the Balkan Peninsula, a high level of variability among and within populations were found. Leaf traits related to leaf size were more variable compared to leaf shape traits, with both influenced by geographic and environmental factors. Consequently,...
With the development of next-generation sequencing technology and bioinformatics tools, the proce... more With the development of next-generation sequencing technology and bioinformatics tools, the process of assembling DNA sequences has become cheaper and easier, especially in the case of much shorter organelle genomes. The number of available DNA sequences of complete chloroplast genomes in public genetic databases is constantly increasing and the data are widely used in plant phylogenetic and biotechnological research. In this work, we investigated possible inconsistencies in the stored form of publicly available chloroplast genome sequence data. The impact of these inconsistencies on the results of the phylogenetic analysis was investigated and the bioinformatic solution to identify and correct inconsistencies was implemented. The whole procedure was demonstrated using five plant families (Apiaceae, Asteraceae, Campanulaceae, Lamiaceae and Rosaceae) as examples.
Landraces represent valuable genetic resources for breeding programmes to produce high-yielding v... more Landraces represent valuable genetic resources for breeding programmes to produce high-yielding varieties adapted to stressful environmental conditions. Although the common bean (Phaseolus vulgaris L.) is an economically important food legume for direct human consumption worldwide, common bean production in Croatia is based almost exclusively on landraces and there is no common bean breeding program. Information on phaseolin type and results of population structure and genetic diversity obtained by analysis of SSR and SNP markers, in combination with the morphological characterization of 174 accessions of 10 common bean landraces (morphotypes), enabled thorough classification of accessions. The accessions were classified into phaseolin type H1 (“S”) of Mesoamerican origin and phaseolin types H2 (“H” or “C”) and H3 (“T”) of Andean origin. By applying distance- and model-based clustering methods to SSR markers, the accessions were classified into two clusters at K = 2 separating the a...
Ramanova spektroskopija je vibracijska spektroskopska metoda koja omogucava analizu spektra razli... more Ramanova spektroskopija je vibracijska spektroskopska metoda koja omogucava analizu spektra razlicitih materijala, ukljucujuci i sekundarne biljne metabolite, ovisno o molekularnoj strukturi i vrsti kemijskih veza. Ova je tehnika prikladna za utvrđivanje i lokalizaciju razlicitih bioaktivnih spojeva kao sto su terpeni, alkaloidi, fenolni spojevi i dr. Kod dalmatinskog buhaca (Tanacetum cinerariifolium /Trevir./ Sch. Bip. ; Asteraceae) aktivna insekticidna tvar je piretrin kojeg cini sest sastavnica (piretrin I i II, cinerin I i II te jasmolin I i II). Utvrđivanje nazocnosti piretrina u ekstraktima cvjetnih glavica dalmatinskog buhaca provodeno je pomocu Ramanove spektroskopije. Prosjecne vrijednosti Ramanovih spektara analiziranih uzoraka ukazuju na nazocnost karotenoida u Ramanovom opsegu od 1000 do 1530 cm-1. Karakteristicni signali visokog intenziteta koji ukazuju na nazocnost piretrina I i II uoceni su u Ramanovim spektrima svih uzoraka na pozicijama 1438, 1600 i 1634 cm-1, dok ...
This study, conducted in Croatia, encompassed for the first time ever populations of the field el... more This study, conducted in Croatia, encompassed for the first time ever populations of the field elm that are solely natural. We studied the genetic diversity and assessed recent bottleneck events and the population structure of 5 natural field elm populations from continental and Mediterranean Croatia, using 5 microsatellite markers. The study results suggest that the observed populations are characterized by a satisfactory amount of heterozygosity, and that the impact of the Dutch elm disease on the amount of genetic diversity in sampled populations is currently negligible. As genetic diversity in forest tree species is slowly diminishing, in the long term there is a danger of the depletion of genetic resources. The danger of inbreeding within populations is low, and the majority of the populations are in Hardy-Weinberg equilibrium. The appearance of bottleneck, initiated by anthropogenic habitat destruction, has been established in only one population. The values of Nei’s standard ...
The systematic status of Dalmatian black pine is still unexplained. This black pine is usually co... more The systematic status of Dalmatian black pine is still unexplained. This black pine is usually considered as subspecies but sometimes also as a separate species. Austrian black pine from Botanical Garden Zagreb, Dalmatian black pine from the mount of Biokovo and Dalmatian black pine from the island of Hvar were used in this study. Two methods of plant molecular systematics have been applied in this work: restriction fragment length polymorphisms of chloroplast DNA (RFLP cpDNA) and random amplified polymorphic DNA (RAPD). The results of RFLP analysis of chloroplast DNA have been showed that there is no differences in restriction fragments among all investigated plants. The RAPD method which is the most appropriate for plant systematic at the species level and below, has been shown DNA polymorphisms between and within investigated populations, distinctly separated Austrian and Dalmatian black pine. The subspecies level for Dalmatian black pine has been strongly supported by these meth...
Jos u davnoj proslosti covjeku su sume pitomoga kestena predstavljale važan izvor razlicitih siro... more Jos u davnoj proslosti covjeku su sume pitomoga kestena predstavljale važan izvor razlicitih sirovina. Intenzivnim koristenjem kestenovih suma i nasada te pojavom raka kestenove kore, sredinom proslog stoljeca doslo je do njihovog propadanja i susenja. Iz tih je razloga u mnogim europskim zemljama pokrenut niz multidisciplinarnih projekata kojima je svrha ocuvanje genskih resursa pitomoga kestena i njegovog povrataka na nekadasnje znacajno mjesto u sumskim ekosustavima. Cilj ovog rada bio je utvrditi geneticku raznolikost populacija europskoga pitomoga kestena u sredisnjoj Europi i zapadnom dijelu Balkanskoga poluotoka. Ukupno je sakupljen 301 uzorak iz 15 populacija, a analiza geneticke raznolikosti provedena je pomocu deset mikrosatelitnih biljega. Ukupno je umnoženo 125 alela, s prosjecnom vrijednosti od 12, 5 alela po lokusu. Aleleno bogatstvo se kretalo od 4, 0 do 6, 1, s prosjecnom vrijednosti od 4, 9. Ni u jednoj populaciji nije utvrđeno statisticki znacajno odstupanje od Har...
The long tradition of green bean (Phaseolus vulgaris L.) cultivation in Croatia has resulted in t... more The long tradition of green bean (Phaseolus vulgaris L.) cultivation in Croatia has resulted in the development of a landraces adapted to specific areas and chosen to suit different dietary habits. The hypothesis of our study was that nutritional quality (content of proteins, sugars, fibres, calcium, potassium and phosphorus in fresh pods) of 14 widely grown Croatian green bean accessions is affected by origin and morphotype. The analysed accessions were grown in non-replicated field plots where morphotypes were classified. The gene pool of adscription (Andean vs. Mesoamerican) of each accession was determined with the phaseolin marker, whereas nutritional quality traits were analysed according to standard analytical methods. The relationships among nutritional quality traits of fresh pods were assessed by Pearson’s correlation coefficient. The univariate ANOVA and a post hoc Tukey test (p0.05) were carried out for each trait to compare the values among accessions. Accessions were ...
Summary Dalmatian or common sage (Salvia offi cinalis L.) is an outcrossing plant species native ... more Summary Dalmatian or common sage (Salvia offi cinalis L.) is an outcrossing plant species native to East Adriatic coast. Random Amplifi ed Polymorphic DNA markers (RAPD) were used to analyze genetic diversity and structure of ten natural populations from the East-Adriatic coastal region. Th e highest genetic diversity was found in populations from the central and south Dalmatia, while the highest frequency down-weighted marker values were found in the northernmost populations and the southern most inland population. Although analysis of molecular variance (AMOVA) revealed that most of the genetic diversity was attributable to diff erences among individuals within populations, highly signifi cant ST values suggested the existence of genetic diff erentiation among populations. By assuming Hardy-Weinberg equilibrium within populations, the calculated FST value among population was moderate. Bayesian model-based clustering method revealed that at K = 2 all individuals belonging to two ...
Dalmatian sage (Salvia officinalis L.) and Greek sage (S. fruticosa Mill.) are two closely relate... more Dalmatian sage (Salvia officinalis L.) and Greek sage (S. fruticosa Mill.) are two closely related species. While Dalmatian sage is naturally widespread on the Apennines and eastern Adriatic coast, Greek sage is naturally widespread in the eastern Mediterranean. The island of Vis is the only place in Croatia where S. fruticosa has been noticed. The presence of this species in Croatia, far away from its natural range, may be related to ancient Greek colonisation of the island in the fourth century BCE. From that time Greek sage probably escaped from gardens and vineyards and successfully naturalised western part of the island. Besides sharing the same habitat on the island of Vis, Dalmatian and Greek sage have the same chromosome number (2n = 14) and their flowering times overlap. The fact that the artificial hybrids between these two species are known and commercially exploited encouraged us to study spontaneous hybridization on the island of Vis. A total of 79 plant samples represe...
Summary Dalmatian sage (Salvia offi cinalis L.) represents one of the most signifi cant medicinal... more Summary Dalmatian sage (Salvia offi cinalis L.) represents one of the most signifi cant medicinal autochthonous species in fl ora of eastern Adriatic coast and islands. It is evergreen outcrossing perennial subshrub with short woody stems that branch extensively and violet fl owers. Apart from being native to Mediterranean karst of west Balkan and Apenine peninsula it is cultivated in numerous countries worldwide with Mediterranean and temperate continental climate. From the earliest times it has been used in traditional medicine in healing gingiva, mouth cavity and the sore throat, against bacterial and fungal infections, for wound treatment, memory enhancement, for treating common cold, against sweating, stomach infl ammation, ulcer formation, etc. Its essential oil has also been used in preservation of food and as spice as it gives both specifi c aroma and promotes digestion of food. Th e essential oil is extremely complex mixture of diff erent active ingredients; however, the th...
Almond-leaved pear (Pyrus spinosa Forssk., Rosaceae) is a scientifically poorly researched and of... more Almond-leaved pear (Pyrus spinosa Forssk., Rosaceae) is a scientifically poorly researched and often overlooked Mediterranean species. It is an insect-pollinated and animal-dispersed spiny, deciduous shrub or a small tree, with high-quality wood and edible fruits. The aim of the study was to assess the phenotypic diversity of almond-leaved pear in the eastern Adriatic region. The examination of phenotypic diversity was based on a morphometric analysis of 17 populations using ten phenotypic traits of leaves. Varieties of multivariate statistical analyses were conducted to evaluate the within- and among-population diversity. In addition, the Mantel tests were used to test the correlations between geographic, environmental, and phenotypic differences among populations. High phenotypic variability was determined both among and within the studied populations. Leaf-size-related traits proved to be the most variable ones, in contrast to more uniform leaf shape traits. Furthermore, three gr...
Short-tooth sage (Salvia brachyodon Vandas) is an endemic and one of the rarest plant species of ... more Short-tooth sage (Salvia brachyodon Vandas) is an endemic and one of the rarest plant species of the Dinaric karst. Although older literature indicates its presence in more localities; only two have been confirmed at the present time; Mt. Orjen (border of Bosnia and Herzegovina and Montenegro) and Peljesac peninsula (Croatia). Due to its very narrow distribution and economically very interesting essential oil composition short-tooth sage might become more endangered in the future. The aim of this research was to determine and compare inter-and intra- population morphological diversity of the only two noted natural populations of short-tooth sage by analyzing 16 quantitative morphological traits. Peljesac population of short-tooth sage showed considerably higher values for traits related to habitus and plant height; while the Mt. Orjen population showed higher values for traits related to the calyx and higher occurrence of sessile glandular hairs on the flower pedicels. When interpre...
Sa??etak: Croatian olive cultivar Lastovka is mainly used for oil production, Its fruity yield is... more Sa??etak: Croatian olive cultivar Lastovka is mainly used for oil production, Its fruity yield is high and regular but due to sensitivity to low temperatures its growing area is limited, with production concentrated on the island of Korcula. Different morphological types have ...
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Papers by Zlatko Liber