Different metabolic steps comprise the pyridine nucleotide cycles in Escherichia coli and in the ... more Different metabolic steps comprise the pyridine nucleotide cycles in Escherichia coli and in the human cell line HeLa D98/AH2. An analysis of the 32P-labeling patterns in vivo reveals that in E. coli, pyrophosphate bond cleavage of intracellular NAD predominates, while in the human cell line, cleavage of the nicotinamide ribose bond predominates. In E. coli, intracellular NAD is processed differently from extracellular NAD. Conversion of intracellular NAD to nicotinic acid mononucleotide (NaMN) can be demonstrated in intact cells. We have also assayed and purified an enzyme, NMN deamidase, which converts NMN to NaMN. These data suggest that in E. coli, the predominant intracellular pyridine nucleotide cycle operative under our experimental conditions is: NAD leads to NMN leads to NaMN leads to NaAD leads to NAD Thus, a metabolic event requiring pyrophosphate bond cleavage of NAD, such as DNA ligation, initiates most NAD turnover. In the human cell line, the data are consistent with the following NAD turnover cycle: (formula, see text) Whereas in E. coli, ADP-ribosylation does not make a quantitatively important contribution, we suggest that in HeLa cells, ADP-ribosylation events initiate NAD turnover.
ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was e... more ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
The marginal seas of East Asia (MSEA hereafter) along the western Pacific, geologically as the in... more The marginal seas of East Asia (MSEA hereafter) along the western Pacific, geologically as the interface between the Pacific Ocean and the Asian land mass, have islands spread from the Bering Sea down to the Indonesian seas consisting mainly of the Japanese, Philippine, and Indonesian Archipelagos. The MSEA is very important to international commerce and global security as linkage of heavily populated megacities with active societal, economical and industrial activities.
The paper discusses the potential of developing a forestry carbon project for the Magbukun ... more The paper discusses the potential of developing a forestry carbon project for the Magbukun Ayta, an indigenous cultural community in the Philippines. The feasibilit y of the forestry carbon project was evaluated based on technical, financial and social aspects. The results of thefeasibility analysis reveal that developing a forestry carbon project for the Magbukun Ay ta of Kanawan, Morang, Bataan is technically and financially feasible. The projected carbon loss in the ancestral domain due to possible deforestation in the old growth forest is a lot higher than expanding second growth forests and brush/ands, thus creating an excellent opportunity for the community to be engaged in a forestry carbon project . The NPV and IRR at the most conservative conditions (i.e., high scenario, price of USD 5t C02-1 and 20% discount rate) are PhP70 .728 million (about USD 1.61 million) and 27%, respectively, which show that the forestry carbon project isfinancially viable. The Magbukun Ayta have also expressed in a resolution that they are amenable to the possibility of being involved in a PES project, specifically on the carbon sequestration potential of their forests, making the forestry carbon project socially acceptable. Likewise, the community's experience with implementing projects and contracts and benefit-sharing and conflict resolution mechanisms place the community in an advantageous position to implement this project . Thus, the project is socially feasible and acceptable. It is therefore recommended that the Magbukun Ayta engage in a forestry carbon project under the voluntary carbon market mechanism.
Three low-cost drying methods (sun, solar, and draft oven) were optimized to produce Spirulina po... more Three low-cost drying methods (sun, solar, and draft oven) were optimized to produce Spirulina powder of optimal quality. Optimization in the pre-dehydration stage included the use of two antioxidants, α-tocopherol and tertiary-butyl hydroquinone (TBHQ), and two blanching methods, microwave and water bath, to inactivate enzymes. The efficiency of the pre-dehydration treatments at minimizing lipid peroxidation were evaluated in terms of
L'invention concerne des peptides de conotoxine, des derives ou des sels de ceux-ci pharmaceu... more L'invention concerne des peptides de conotoxine, des derives ou des sels de ceux-ci pharmaceutiquement acceptables. La presente invention porte egalement sur l'utilisation de ce peptide, de ses derives et des sels de ceux-ci pharmaceutiquement acceptables pour le traitement de maladies associees a des canaux ioniques sensibles au voltage, a des canaux ioniques sensibles a un ligand et/ou a des recepteurs. Cette invention concerne aussi des sequences d'acide nucleique codant les peptides de conotoxine et codant des propeptides, ainsi que ces propeptides.
Different metabolic steps comprise the pyridine nucleotide cycles in Escherichia coli and in the ... more Different metabolic steps comprise the pyridine nucleotide cycles in Escherichia coli and in the human cell line HeLa D98/AH2. An analysis of the 32P-labeling patterns in vivo reveals that in E. coli, pyrophosphate bond cleavage of intracellular NAD predominates, while in the human cell line, cleavage of the nicotinamide ribose bond predominates. In E. coli, intracellular NAD is processed differently from extracellular NAD. Conversion of intracellular NAD to nicotinic acid mononucleotide (NaMN) can be demonstrated in intact cells. We have also assayed and purified an enzyme, NMN deamidase, which converts NMN to NaMN. These data suggest that in E. coli, the predominant intracellular pyridine nucleotide cycle operative under our experimental conditions is: NAD leads to NMN leads to NaMN leads to NaAD leads to NAD Thus, a metabolic event requiring pyrophosphate bond cleavage of NAD, such as DNA ligation, initiates most NAD turnover. In the human cell line, the data are consistent with the following NAD turnover cycle: (formula, see text) Whereas in E. coli, ADP-ribosylation does not make a quantitatively important contribution, we suggest that in HeLa cells, ADP-ribosylation events initiate NAD turnover.
ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was e... more ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
The marginal seas of East Asia (MSEA hereafter) along the western Pacific, geologically as the in... more The marginal seas of East Asia (MSEA hereafter) along the western Pacific, geologically as the interface between the Pacific Ocean and the Asian land mass, have islands spread from the Bering Sea down to the Indonesian seas consisting mainly of the Japanese, Philippine, and Indonesian Archipelagos. The MSEA is very important to international commerce and global security as linkage of heavily populated megacities with active societal, economical and industrial activities.
The paper discusses the potential of developing a forestry carbon project for the Magbukun ... more The paper discusses the potential of developing a forestry carbon project for the Magbukun Ayta, an indigenous cultural community in the Philippines. The feasibilit y of the forestry carbon project was evaluated based on technical, financial and social aspects. The results of thefeasibility analysis reveal that developing a forestry carbon project for the Magbukun Ay ta of Kanawan, Morang, Bataan is technically and financially feasible. The projected carbon loss in the ancestral domain due to possible deforestation in the old growth forest is a lot higher than expanding second growth forests and brush/ands, thus creating an excellent opportunity for the community to be engaged in a forestry carbon project . The NPV and IRR at the most conservative conditions (i.e., high scenario, price of USD 5t C02-1 and 20% discount rate) are PhP70 .728 million (about USD 1.61 million) and 27%, respectively, which show that the forestry carbon project isfinancially viable. The Magbukun Ayta have also expressed in a resolution that they are amenable to the possibility of being involved in a PES project, specifically on the carbon sequestration potential of their forests, making the forestry carbon project socially acceptable. Likewise, the community's experience with implementing projects and contracts and benefit-sharing and conflict resolution mechanisms place the community in an advantageous position to implement this project . Thus, the project is socially feasible and acceptable. It is therefore recommended that the Magbukun Ayta engage in a forestry carbon project under the voluntary carbon market mechanism.
Three low-cost drying methods (sun, solar, and draft oven) were optimized to produce Spirulina po... more Three low-cost drying methods (sun, solar, and draft oven) were optimized to produce Spirulina powder of optimal quality. Optimization in the pre-dehydration stage included the use of two antioxidants, α-tocopherol and tertiary-butyl hydroquinone (TBHQ), and two blanching methods, microwave and water bath, to inactivate enzymes. The efficiency of the pre-dehydration treatments at minimizing lipid peroxidation were evaluated in terms of
L'invention concerne des peptides de conotoxine, des derives ou des sels de ceux-ci pharmaceu... more L'invention concerne des peptides de conotoxine, des derives ou des sels de ceux-ci pharmaceutiquement acceptables. La presente invention porte egalement sur l'utilisation de ce peptide, de ses derives et des sels de ceux-ci pharmaceutiquement acceptables pour le traitement de maladies associees a des canaux ioniques sensibles au voltage, a des canaux ioniques sensibles a un ligand et/ou a des recepteurs. Cette invention concerne aussi des sequences d'acide nucleique codant les peptides de conotoxine et codant des propeptides, ainsi que ces propeptides.
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