Adsorption of ØX174 to cell wall fragments fromE. coli labeled with3H-diaminopimel ic acid result... more Adsorption of ØX174 to cell wall fragments fromE. coli labeled with3H-diaminopimel ic acid results in limited degradation of murein due to stimulation of autolysis. Pure murein was not degraded by ØX174.
Bacterial ghosts are empty cell envelopes originating from Gram-negative bacteria. They have a na... more Bacterial ghosts are empty cell envelopes originating from Gram-negative bacteria. They have a natural outer surface make-up which provides them with the original targeting functions of the bacteria they are derived from and are thus able to bind to and/or are taken up by specific cells or tissues of animal, human or plant origin. The extended bacterial ghost system represents a platform technology for creating new qualities in non-living carriers which can be used for the specific targeting of drugs, DNA or other compounds to overcome toxic or non-desired obstacles. Freeze dried bacterial ghosts are stable without the requirement of a cold chain and can be effectively administered orally and aerogenically as drug carriers. The new system is an alternative to liposomes and may have an advantage due to its higher specificity for targeting specific tissues, its easy method of production and its versatility in entrapping and packaging various compounds in different compartments of the carriers.
A comparison was made of the cell surface glycoproteins of four human cell lines, namely a giant ... more A comparison was made of the cell surface glycoproteins of four human cell lines, namely a giant tumor of bone cell line, an osteosarcoma line, a fibrosarcoma line and a human fibroblast line. The cells were labeled by lactoperoxidase catalyzed iodination and the glycoproteins extracted by 0.5% Triton X-100 were bound to lentil-lectin and subsequently analyzed by SDS gel electrophoresis. While the cell lines examined shared a series of common glycoproteins, it was found that the giant cell tumor line and the fibrosarcoma lines exhibited a higher degree of homology than the other cell lines.
B. stearothermophilus PV72 is a thermophilic gram-positive bacterium, which is surrounded by a he... more B. stearothermophilus PV72 is a thermophilic gram-positive bacterium, which is surrounded by a hexagonally-arranged (p6) S-layer (Sleytr et al., 1986). As estimated by SDS-PAGE, the S-layer protein of B. stearothermophilus has a Mr = 130 kDa (Messner et al., 1984). The S-layer can be dissociated into subunits which have the ability to self-assemble into lattices identical to those observed on intact cells (Sleytr, 1976).
One feature common to many bacteria, regardless of their phylogenetic origin within the kingdoms ... more One feature common to many bacteria, regardless of their phylogenetic origin within the kingdoms Eucarya or Archaea, is the presence of a regularly ordered (glyco)protein border as the outermost macromolecular layer of the cell envelope. A recent list of organisms with such crystalline surface layers (S-layers) cites approximately 300 different prokaryotic species (Messner and Sleytr, 1992). However, most of the S-layers have only been described by electron microscopical or biochemical investigations and DNA sequence data of the corresponding genes are available for very few species (approximately 20). Here we report on the structural and functional properties of the S-layer of Bacillus stearothermophilus strain PV72 which have been deduced from computer analysis of DNA-sequence data (for sequencing details of the gene see the contribution by Kuen, Sara, Sleytr, and Lubitz in this book).
ABSTRACT InEscherichia coli C infected with bacteriophage X174, the cytoplasmic and outer membran... more ABSTRACT InEscherichia coli C infected with bacteriophage X174, the cytoplasmic and outer membranes of the host bacterium exhibit various alterations in their protein composition as revealed by sodium dodecyl sulfate gel electrophoresis of purified membranes. These alterations result mainly from the action of the lysis gene product of the phage. One effect of the changes occurring in the membranes results in different rates of release of wild-type phage and its lysis-negative mutant from glycine spheroplasts. The activity of phospho-MurNAc-pentapeptide translocase, an enzyme involved in murein synthesis and located in the cytoplasmic membrane, is unimpaired by these alterations.
Adsorption of ØX174 to cell wall fragments fromE. coli labeled with3H-diaminopimel ic acid result... more Adsorption of ØX174 to cell wall fragments fromE. coli labeled with3H-diaminopimel ic acid results in limited degradation of murein due to stimulation of autolysis. Pure murein was not degraded by ØX174.
Bacterial ghosts are empty cell envelopes originating from Gram-negative bacteria. They have a na... more Bacterial ghosts are empty cell envelopes originating from Gram-negative bacteria. They have a natural outer surface make-up which provides them with the original targeting functions of the bacteria they are derived from and are thus able to bind to and/or are taken up by specific cells or tissues of animal, human or plant origin. The extended bacterial ghost system represents a platform technology for creating new qualities in non-living carriers which can be used for the specific targeting of drugs, DNA or other compounds to overcome toxic or non-desired obstacles. Freeze dried bacterial ghosts are stable without the requirement of a cold chain and can be effectively administered orally and aerogenically as drug carriers. The new system is an alternative to liposomes and may have an advantage due to its higher specificity for targeting specific tissues, its easy method of production and its versatility in entrapping and packaging various compounds in different compartments of the carriers.
A comparison was made of the cell surface glycoproteins of four human cell lines, namely a giant ... more A comparison was made of the cell surface glycoproteins of four human cell lines, namely a giant tumor of bone cell line, an osteosarcoma line, a fibrosarcoma line and a human fibroblast line. The cells were labeled by lactoperoxidase catalyzed iodination and the glycoproteins extracted by 0.5% Triton X-100 were bound to lentil-lectin and subsequently analyzed by SDS gel electrophoresis. While the cell lines examined shared a series of common glycoproteins, it was found that the giant cell tumor line and the fibrosarcoma lines exhibited a higher degree of homology than the other cell lines.
B. stearothermophilus PV72 is a thermophilic gram-positive bacterium, which is surrounded by a he... more B. stearothermophilus PV72 is a thermophilic gram-positive bacterium, which is surrounded by a hexagonally-arranged (p6) S-layer (Sleytr et al., 1986). As estimated by SDS-PAGE, the S-layer protein of B. stearothermophilus has a Mr = 130 kDa (Messner et al., 1984). The S-layer can be dissociated into subunits which have the ability to self-assemble into lattices identical to those observed on intact cells (Sleytr, 1976).
One feature common to many bacteria, regardless of their phylogenetic origin within the kingdoms ... more One feature common to many bacteria, regardless of their phylogenetic origin within the kingdoms Eucarya or Archaea, is the presence of a regularly ordered (glyco)protein border as the outermost macromolecular layer of the cell envelope. A recent list of organisms with such crystalline surface layers (S-layers) cites approximately 300 different prokaryotic species (Messner and Sleytr, 1992). However, most of the S-layers have only been described by electron microscopical or biochemical investigations and DNA sequence data of the corresponding genes are available for very few species (approximately 20). Here we report on the structural and functional properties of the S-layer of Bacillus stearothermophilus strain PV72 which have been deduced from computer analysis of DNA-sequence data (for sequencing details of the gene see the contribution by Kuen, Sara, Sleytr, and Lubitz in this book).
ABSTRACT InEscherichia coli C infected with bacteriophage X174, the cytoplasmic and outer membran... more ABSTRACT InEscherichia coli C infected with bacteriophage X174, the cytoplasmic and outer membranes of the host bacterium exhibit various alterations in their protein composition as revealed by sodium dodecyl sulfate gel electrophoresis of purified membranes. These alterations result mainly from the action of the lysis gene product of the phage. One effect of the changes occurring in the membranes results in different rates of release of wild-type phage and its lysis-negative mutant from glycine spheroplasts. The activity of phospho-MurNAc-pentapeptide translocase, an enzyme involved in murein synthesis and located in the cytoplasmic membrane, is unimpaired by these alterations.
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Papers by Werner Lubitz