Dr. Münir AktaşDepartment of Parasitology, Veterinary Medicine, Firat University, 23119, Elazig, Turkey Munir Aktaş earned his undergraduate and MSci degrees at the University of Firat in Turkey. Subsequently he served on the faculty of Veterinary Medicine, first as an assistant of Parasitology until 1997. In 1997, Munir Aktaş received his PhD degree from the University of Firat. Currently, Dr Aktas is an Professor in Department of Parasitology, Faculty of Veterinary Medicine, Firat University, Turkey. In the course of his PhD thesis research, Dr. Aktaş conducted fields studies in cattle vaccinated against tropical theileriosis. In parallel he worked in ticks and tick-borne diseases (babesiosis, theileriosis, anaplasmosis). Dr. Munir Aktaş is a member of the Turkish Society of Parasitology. At national and international levels, he has served as a member of Turkish Research Grant Review panel.
Eurasian Journal of Veterinary Sciences, Apr 4, 2004
This study was carried oul in eats in Elazig. A total of 36 eals were examined for the presence o... more This study was carried oul in eats in Elazig. A total of 36 eals were examined for the presence of anti-Toxoplasma gondii antibodies using Sabin-Felclman dye test (SF). Anti-Toxoplasma gondii antibodies were detected in 20 (55.5%) of the eats. Titers ol 1/16, 1164 were found in 9 (45%) and 11 (55%) ol the seropozitive ea ts res-pectively, Toxoplasma gondii oocyst was not found in the faeces examination.
Türkiye parazitolojii dergisi / Türkiye Parazitoloji Derneği = Acta parasitologica Turcica / Turkish Society for Parasitology, 2007
Tams1 is a merozoite surface antigen of Theileria annulata. Genetical variations of Tams1 make st... more Tams1 is a merozoite surface antigen of Theileria annulata. Genetical variations of Tams1 make studies of vaccine and diagnostic tests like ELISA difficult. In this study, Tams1 genes of 89 T. annulata isolates obtained from natural infected cattle in Elazig and Bingöl regions were tested with PCR-RFLP. Six different restriction profiles (a, b, c, d, e, f) were detected. The number of restriction profiles of 89 samples was found to be as follows: 78(a), 2(b), 2(c), 5(d), 1(e), and 1(f).
Türkiye parazitolojii dergisi / Türkiye Parazitoloji Derneği = Acta parasitologica Turcica / Turkish Society for Parasitology, 2007
This study was carried out to investigate Theileria annulata and T. buffeli/orientalis in cattle ... more This study was carried out to investigate Theileria annulata and T. buffeli/orientalis in cattle in the region of Erzincan using reverse line blotting (RLB) and microscopical examination. A total of 123 blood samples and thin blood smears were collected from cattle in distinct locations. Thin blood smears were microscopically examined for Theileria piroplasms. The 18S SSU rRNA gene in the DNA of Theileria spp extracted from blood was amplified and used in RLB. For this purpose, PCR products were hybridized with specific probes for over-all Theileria spp., T. annulata and T. buffeli/orientalis as well as Babesia spp. While Theileria spp. were observed in 14 out of 123 cattle, (11.38 %) during microscopical examination, T. annulata was detected in 19 (15.45%) cattle and T. buffeli/orientalis, in 12 (9.76%) by RLB, respectively. Mixed infection was also detected in three samples.
Türkiye parazitolojii dergisi / Türkiye Parazitoloji Derneği = Acta parasitologica Turcica / Turkish Society for Parasitology, 2005
This study was carried out in order to investigate the presence of Theileria ovis in small rumina... more This study was carried out in order to investigate the presence of Theileria ovis in small ruminants in the Elazig region between April-October 2004. A total of 164 whole blood and thin blood smears (from 103 sheep and 61 goats) were collected from 15 flocks in different locations. T. ovis piroplasm DNA extracted from sheep and goats' blood was amplified by the polymerase chain reaction (PCR) using specific primers. Thin blood smears were examined for Theileria piroplasms by microscopic examination. In the examination of DNA extracted from 103 sheep and 61 goats, amplification with the molecular length 520 base pair was obtained in 67.96% (70/103) and 1.63% (1/61), respectively. In the microscopic examination of thin blood smears, Theileria spp. were observed in 40 out of 103 sheep (38.83%), but Theileria spp. were not seen in goats. The difference between microscopic examination and the PCR results was statistically significant (p < 0.01).
Türkiye parazitolojii dergisi / Türkiye Parazitoloji Derneği = Acta parasitologica Turcica / Turkish Society for Parasitology, 2008
The species causing theileriosis in cattle in Turkey are Theileria annulata and T. buffeli. While... more The species causing theileriosis in cattle in Turkey are Theileria annulata and T. buffeli. While T. buffeli is low in pathogenicity or non-pathogenic , T. annulata is very pathogenic and causes tropical theileriosis with high morbidity and mortality in cattle. In this study, a multiplex PCR was used for a simultaneous diagnosis of these species. Genes for the merozoite surface antigen (Tams 1) and the major piroplasm surface protein (MPSP) were amplified with PCR for T. annulata and T. buffeli, respectively. It was found that both single and mixed infection with T. annulata and T. buffeli could be diagnosed with multiplex PCR.
References AYERS, E., CAMERON, E., KEMP, R., LEITCH, H., MOLLISON, A., MUIR, I., REID, H., SMITH,... more References AYERS, E., CAMERON, E., KEMP, R., LEITCH, H., MOLLISON, A., MUIR, I., REID, H., SMITH, D. & SPROAT, J. (2001) Oral lesions in sheep and cattle in Dumfries and Galloway. Veterinary Record 148, 720-723 DE LA RUA, R., WATKINS, G. H. & WATSON, P. J. (2001) Idiopathic mouth ulcers in sheep. Veterinary Record 149, 30-31 DONALDSON, A. I.,ALEXANDERSEN, S., SORENSEN, J. H. & MIKKELSEN, T. (2001) Relative risks of the uncontrollable (airborne) spread of FMD by different species. Veterinary Record 148, 602-604 DONALDSON, A. I. & SELLERS, R. F. (2000) Foot-and-mouth disease. In Diseases of Sheep. 3rd edn. Eds W. B. Martin, I. D. Aitken. Oxford, Blackwell Science. pp 254-258 SANSON, R. L. (1994) The epidemiology of foot-and-mouth disease: implications for New Zealand. New Zealand Veterinary Journal 42, 41-53 Number Of samples Thin bld smear IFAT PCR
ABSTRACT This study provides the first molecular data on infection Theileria of sheep in Turkey. ... more ABSTRACT This study provides the first molecular data on infection Theileria of sheep in Turkey. A total of 218 blood samples were collected from sheep in five distinct geographical locations of eastern Turkey. Theileria piroplasm DNAs, extracted from sheep blood, were subjected to the polymerase chain reaction (PCR) procedures, using specific primers for Theileria spp. and Theileria lestoquardi. Blood smears were examined for Theileria piroplasms by microscopic observation. In PCR analysis, the expected amplicons were obtained from 90 (41.2%) blood samples with a molecular size of 1098 bp for Theileria spp. whereas none were amplified by Theileria lestoquardi-specific primers. Piroplasms of Theileria spp. were detected in 34 (15.5%) of the samples.
Turkish Journal of Veterinary & Animal Sciences, 2001
This study was carried out to determine Hyalommaticks and the natural infections of Hyalommaspeci... more This study was carried out to determine Hyalommaticks and the natural infections of Hyalommaspecies with Theileria annulata in cattle and their shelters in Malatya province between May 1998 and January 1999. Selected areas in Malatya province were visited to collect ticks from cows and their shelters during the study. Salivary glands of Hyalommaspecies were dissected and thin films were prepared. The nymphs collected from cattle and their shelters were kept at 26 °C and 85% relative humudity. Unfed adults of Hyalom- maspecies taken from engorged nymphs and collected from shelters were kept at 37°C and 85% relative humudity for at least five days,and then salivary glands were dissected. All films prepared from salivary glands were stained with Methylegreen/Pyronin stain and examined for T.annulata infection under microscope. A total of 1633 Hyalommaticks were collected from cattle,and their species were determined and the numbers were as follows: Hyalomma anatolicum anatolicum 990 (6...
Turkish Journal of Veterinary & Animal Sciences, 1996
... veterinary@tubitak.gov.tr. Scientific Journals Home Page. Turkish Journal of Veterinary and A... more ... veterinary@tubitak.gov.tr. Scientific Journals Home Page. Turkish Journal of Veterinary and Animal Sciences. Pneumocystis carinii (Delanoe and Delanoe, 1912) Infections in Some Bird Species. Edip ÖZER, Cem Ecmel ŞAKİ, Murat SEVGİLİ, Nursel AKSIN, Münir AKTAŞ. ...
Eurasian Journal of Veterinary Sciences, Apr 4, 2004
This study was carried oul in eats in Elazig. A total of 36 eals were examined for the presence o... more This study was carried oul in eats in Elazig. A total of 36 eals were examined for the presence of anti-Toxoplasma gondii antibodies using Sabin-Felclman dye test (SF). Anti-Toxoplasma gondii antibodies were detected in 20 (55.5%) of the eats. Titers ol 1/16, 1164 were found in 9 (45%) and 11 (55%) ol the seropozitive ea ts res-pectively, Toxoplasma gondii oocyst was not found in the faeces examination.
Türkiye parazitolojii dergisi / Türkiye Parazitoloji Derneği = Acta parasitologica Turcica / Turkish Society for Parasitology, 2007
Tams1 is a merozoite surface antigen of Theileria annulata. Genetical variations of Tams1 make st... more Tams1 is a merozoite surface antigen of Theileria annulata. Genetical variations of Tams1 make studies of vaccine and diagnostic tests like ELISA difficult. In this study, Tams1 genes of 89 T. annulata isolates obtained from natural infected cattle in Elazig and Bingöl regions were tested with PCR-RFLP. Six different restriction profiles (a, b, c, d, e, f) were detected. The number of restriction profiles of 89 samples was found to be as follows: 78(a), 2(b), 2(c), 5(d), 1(e), and 1(f).
Türkiye parazitolojii dergisi / Türkiye Parazitoloji Derneği = Acta parasitologica Turcica / Turkish Society for Parasitology, 2007
This study was carried out to investigate Theileria annulata and T. buffeli/orientalis in cattle ... more This study was carried out to investigate Theileria annulata and T. buffeli/orientalis in cattle in the region of Erzincan using reverse line blotting (RLB) and microscopical examination. A total of 123 blood samples and thin blood smears were collected from cattle in distinct locations. Thin blood smears were microscopically examined for Theileria piroplasms. The 18S SSU rRNA gene in the DNA of Theileria spp extracted from blood was amplified and used in RLB. For this purpose, PCR products were hybridized with specific probes for over-all Theileria spp., T. annulata and T. buffeli/orientalis as well as Babesia spp. While Theileria spp. were observed in 14 out of 123 cattle, (11.38 %) during microscopical examination, T. annulata was detected in 19 (15.45%) cattle and T. buffeli/orientalis, in 12 (9.76%) by RLB, respectively. Mixed infection was also detected in three samples.
Türkiye parazitolojii dergisi / Türkiye Parazitoloji Derneği = Acta parasitologica Turcica / Turkish Society for Parasitology, 2005
This study was carried out in order to investigate the presence of Theileria ovis in small rumina... more This study was carried out in order to investigate the presence of Theileria ovis in small ruminants in the Elazig region between April-October 2004. A total of 164 whole blood and thin blood smears (from 103 sheep and 61 goats) were collected from 15 flocks in different locations. T. ovis piroplasm DNA extracted from sheep and goats' blood was amplified by the polymerase chain reaction (PCR) using specific primers. Thin blood smears were examined for Theileria piroplasms by microscopic examination. In the examination of DNA extracted from 103 sheep and 61 goats, amplification with the molecular length 520 base pair was obtained in 67.96% (70/103) and 1.63% (1/61), respectively. In the microscopic examination of thin blood smears, Theileria spp. were observed in 40 out of 103 sheep (38.83%), but Theileria spp. were not seen in goats. The difference between microscopic examination and the PCR results was statistically significant (p < 0.01).
Türkiye parazitolojii dergisi / Türkiye Parazitoloji Derneği = Acta parasitologica Turcica / Turkish Society for Parasitology, 2008
The species causing theileriosis in cattle in Turkey are Theileria annulata and T. buffeli. While... more The species causing theileriosis in cattle in Turkey are Theileria annulata and T. buffeli. While T. buffeli is low in pathogenicity or non-pathogenic , T. annulata is very pathogenic and causes tropical theileriosis with high morbidity and mortality in cattle. In this study, a multiplex PCR was used for a simultaneous diagnosis of these species. Genes for the merozoite surface antigen (Tams 1) and the major piroplasm surface protein (MPSP) were amplified with PCR for T. annulata and T. buffeli, respectively. It was found that both single and mixed infection with T. annulata and T. buffeli could be diagnosed with multiplex PCR.
References AYERS, E., CAMERON, E., KEMP, R., LEITCH, H., MOLLISON, A., MUIR, I., REID, H., SMITH,... more References AYERS, E., CAMERON, E., KEMP, R., LEITCH, H., MOLLISON, A., MUIR, I., REID, H., SMITH, D. & SPROAT, J. (2001) Oral lesions in sheep and cattle in Dumfries and Galloway. Veterinary Record 148, 720-723 DE LA RUA, R., WATKINS, G. H. & WATSON, P. J. (2001) Idiopathic mouth ulcers in sheep. Veterinary Record 149, 30-31 DONALDSON, A. I.,ALEXANDERSEN, S., SORENSEN, J. H. & MIKKELSEN, T. (2001) Relative risks of the uncontrollable (airborne) spread of FMD by different species. Veterinary Record 148, 602-604 DONALDSON, A. I. & SELLERS, R. F. (2000) Foot-and-mouth disease. In Diseases of Sheep. 3rd edn. Eds W. B. Martin, I. D. Aitken. Oxford, Blackwell Science. pp 254-258 SANSON, R. L. (1994) The epidemiology of foot-and-mouth disease: implications for New Zealand. New Zealand Veterinary Journal 42, 41-53 Number Of samples Thin bld smear IFAT PCR
ABSTRACT This study provides the first molecular data on infection Theileria of sheep in Turkey. ... more ABSTRACT This study provides the first molecular data on infection Theileria of sheep in Turkey. A total of 218 blood samples were collected from sheep in five distinct geographical locations of eastern Turkey. Theileria piroplasm DNAs, extracted from sheep blood, were subjected to the polymerase chain reaction (PCR) procedures, using specific primers for Theileria spp. and Theileria lestoquardi. Blood smears were examined for Theileria piroplasms by microscopic observation. In PCR analysis, the expected amplicons were obtained from 90 (41.2%) blood samples with a molecular size of 1098 bp for Theileria spp. whereas none were amplified by Theileria lestoquardi-specific primers. Piroplasms of Theileria spp. were detected in 34 (15.5%) of the samples.
Turkish Journal of Veterinary & Animal Sciences, 2001
This study was carried out to determine Hyalommaticks and the natural infections of Hyalommaspeci... more This study was carried out to determine Hyalommaticks and the natural infections of Hyalommaspecies with Theileria annulata in cattle and their shelters in Malatya province between May 1998 and January 1999. Selected areas in Malatya province were visited to collect ticks from cows and their shelters during the study. Salivary glands of Hyalommaspecies were dissected and thin films were prepared. The nymphs collected from cattle and their shelters were kept at 26 °C and 85% relative humudity. Unfed adults of Hyalom- maspecies taken from engorged nymphs and collected from shelters were kept at 37°C and 85% relative humudity for at least five days,and then salivary glands were dissected. All films prepared from salivary glands were stained with Methylegreen/Pyronin stain and examined for T.annulata infection under microscope. A total of 1633 Hyalommaticks were collected from cattle,and their species were determined and the numbers were as follows: Hyalomma anatolicum anatolicum 990 (6...
Turkish Journal of Veterinary & Animal Sciences, 1996
... veterinary@tubitak.gov.tr. Scientific Journals Home Page. Turkish Journal of Veterinary and A... more ... veterinary@tubitak.gov.tr. Scientific Journals Home Page. Turkish Journal of Veterinary and Animal Sciences. Pneumocystis carinii (Delanoe and Delanoe, 1912) Infections in Some Bird Species. Edip ÖZER, Cem Ecmel ŞAKİ, Murat SEVGİLİ, Nursel AKSIN, Münir AKTAŞ. ...
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