Salt Stress, Microbes, and Plant Interactions: Mechanisms and Molecular Approaches, 2019
Soil salinity is one of the main factors restricting crop production throughout the world. Variou... more Soil salinity is one of the main factors restricting crop production throughout the world. Various salt tolerance traits and the genes controlling these traits are responsible for coping with salinity stress in plants. These coping mechanisms include osmotic tolerance, ion exclusion, and tissue tolerance. Plants exposed to salinity stress sense the stress conditions, convey specific stimuli signals, and initiate responses against stress through the activation of tolerance mechanisms that include multiple genes and pathways. Advances in our understanding of the genetic responses of plants to salinity and their connections with yield improvement are essential for attaining sustainable agriculture. Although a wide range of studies have been conducted that demonstrate genetic variations in response to salinity stress, numerous questions need to be answered to fully understand plant tolerance to salt stress. This chapter provides an overview of previous studies on the genetic control of ...
Magnaporthe oryzae is a rice blast fungus and plant pathogen that causes a serious rice disease a... more Magnaporthe oryzae is a rice blast fungus and plant pathogen that causes a serious rice disease and, therefore, poses a threat to the world’s second most important food security crop. Plant transformation technology has become an adaptable system for cultivar improvement and to functionally analyze genes in plants. The objective of this study was to determine the effects (through over-expressing and using the CaMV 35S promoter) of Pikh on MR219 resistance because it is a rice variety that is susceptible to the blast fungus pathotype P7.2. Thus, a full coding DNA sequence (CDS) of the Pikh gene, 1206 bp in length, was obtained through amplifying the cDNA template from a PH9-resistant rice variety using a specific primer. Agrobacterium-mediated transformation technology was also used to introduce the Pikh gene into the MR219 callus. Subsequently, transgenic plants were evaluated from the DNA to protein stages using polymerase chain reaction (PCR), semi-quantitative RT-PCR, real-time q...
Rice blast is the most destructive disease to rice production globally. The objective of this rev... more Rice blast is the most destructive disease to rice production globally. The objective of this review is to know the fundamentals of rice blast disease and to know the different methods for controlling blast disease. Rice blast disease has been recognised in more than 85 rice-producing countries worldwide. Currently, more than 100 R genes for blast resistance have been identified in rice. These resistance genes can be introgressed into a susceptible variety through marker-assisted backcrossing. Infested residues and seeds are the primary inoculum sources to spread the disease. Considering the importance of this disease, various management approaches have been practiced to control blast disease. The use of resistant varieties is an important measure to manage the disease. This review will provide use fulfacts about the pathogen and its epidemiology, assessment of resistance genes and effective control measure of rice blast disease through breeding and management. This update informati...
Molecular marker advancement and utilization to exploit and identify genetic diversity are the mo... more Molecular marker advancement and utilization to exploit and identify genetic diversity are the most key developments in molecular genetic studies. Molecular markers are the most common tool for exploring plant genetic diversity and area pre- requisite for gene mapping, segregation and genetic analysis, forensic assessment, phylogenetic analysis, and the achievement of many other biological goals. Despite the development of several molecular marker types that are regularly applied in plant breeding, majority of these markers are confined in their functions due to their limited accessibility and the cost of analyses carried out on a large scale. However, the advent of sequencing technologies, including next-generation sequencing (NGS) and genotyping by sequencing (GBS), has transformed plant breeding via SSR and SNP development. To date, different types of sequencing technologies have been generated and reviewed. This chapter provides insight into the spectrum of molecular marker-base...
Salt Stress, Microbes, and Plant Interactions: Mechanisms and Molecular Approaches, 2019
Soil salinity is one of the main factors restricting crop production throughout the world. Variou... more Soil salinity is one of the main factors restricting crop production throughout the world. Various salt tolerance traits and the genes controlling these traits are responsible for coping with salinity stress in plants. These coping mechanisms include osmotic tolerance, ion exclusion, and tissue tolerance. Plants exposed to salinity stress sense the stress conditions, convey specific stimuli signals, and initiate responses against stress through the activation of tolerance mechanisms that include multiple genes and pathways. Advances in our understanding of the genetic responses of plants to salinity and their connections with yield improvement are essential for attaining sustainable agriculture. Although a wide range of studies have been conducted that demonstrate genetic variations in response to salinity stress, numerous questions need to be answered to fully understand plant tolerance to salt stress. This chapter provides an overview of previous studies on the genetic control of ...
Magnaporthe oryzae is a rice blast fungus and plant pathogen that causes a serious rice disease a... more Magnaporthe oryzae is a rice blast fungus and plant pathogen that causes a serious rice disease and, therefore, poses a threat to the world’s second most important food security crop. Plant transformation technology has become an adaptable system for cultivar improvement and to functionally analyze genes in plants. The objective of this study was to determine the effects (through over-expressing and using the CaMV 35S promoter) of Pikh on MR219 resistance because it is a rice variety that is susceptible to the blast fungus pathotype P7.2. Thus, a full coding DNA sequence (CDS) of the Pikh gene, 1206 bp in length, was obtained through amplifying the cDNA template from a PH9-resistant rice variety using a specific primer. Agrobacterium-mediated transformation technology was also used to introduce the Pikh gene into the MR219 callus. Subsequently, transgenic plants were evaluated from the DNA to protein stages using polymerase chain reaction (PCR), semi-quantitative RT-PCR, real-time q...
Rice blast is the most destructive disease to rice production globally. The objective of this rev... more Rice blast is the most destructive disease to rice production globally. The objective of this review is to know the fundamentals of rice blast disease and to know the different methods for controlling blast disease. Rice blast disease has been recognised in more than 85 rice-producing countries worldwide. Currently, more than 100 R genes for blast resistance have been identified in rice. These resistance genes can be introgressed into a susceptible variety through marker-assisted backcrossing. Infested residues and seeds are the primary inoculum sources to spread the disease. Considering the importance of this disease, various management approaches have been practiced to control blast disease. The use of resistant varieties is an important measure to manage the disease. This review will provide use fulfacts about the pathogen and its epidemiology, assessment of resistance genes and effective control measure of rice blast disease through breeding and management. This update informati...
Molecular marker advancement and utilization to exploit and identify genetic diversity are the mo... more Molecular marker advancement and utilization to exploit and identify genetic diversity are the most key developments in molecular genetic studies. Molecular markers are the most common tool for exploring plant genetic diversity and area pre- requisite for gene mapping, segregation and genetic analysis, forensic assessment, phylogenetic analysis, and the achievement of many other biological goals. Despite the development of several molecular marker types that are regularly applied in plant breeding, majority of these markers are confined in their functions due to their limited accessibility and the cost of analyses carried out on a large scale. However, the advent of sequencing technologies, including next-generation sequencing (NGS) and genotyping by sequencing (GBS), has transformed plant breeding via SSR and SNP development. To date, different types of sequencing technologies have been generated and reviewed. This chapter provides insight into the spectrum of molecular marker-base...
Uploads
Papers by Mahbod Sahebi