Revista De Obstetricia Y Ginecologia De Venezuela, Dec 1, 2013
Objetivo: Deteccion subclinica del virus de papiloma humano en muestras de pacientes diagnosticad... more Objetivo: Deteccion subclinica del virus de papiloma humano en muestras de pacientes diagnosticadas con ectropion cervical, para evaluar la prevalencia de la infeccion viral asociada a dicha condicion. Metodos: El ADN fue extraido utilizando solventes organicos (fenol/cloroformo-alcohol isoamilico). La deteccion del virus de papiloma humano se realizo mediante PCR con iniciadores genericos MY09/MY11 y para la tipificacion de las muestras positivas se utilizo un kit comercial de PCR multiple. Ambiente: Laboratorio de Genetica Molecular-Instituto de Oncologia y Hematologia. Resultados: Se obtuvo una positividad de 26 % (13/50 muestras analizadas) para la presencia de ADN del virus de papiloma humano. De las muestras positivas, 38,45 % resulto virus de papiloma humano de alto riesgo oncogenico (tipo 16 o 18), mientras que otro 38,45 % correspondio a virus de papiloma humano de bajo riesgo (tipos 6, 11 o infeccion mixta 6/11) y 23,07 % no pudo ser tipificado con la metodologia utilizada. Conclusion: Aunque este resultado no fue estadisticamente significativo, senala la necesidad de mayor seguimiento clinico de las pacientes positivas, especialmente aquellos casos correspondientes a virus de papiloma humano de alto riesgo oncogenico, ya que presentan mayor probabilidad de desarrollar cancer cervical. Objective: In this study was conducted subclinical human papillomavirus detection in samples from patients diagnosed with cervical ectropion to assess the prevalence of human papillomavirus infection associated with this condition. Methods: DNA was extracted with organic solvents (phenol /chloroform- isoamylic). Human papillomavirus detection was performed by PCR with generic primers MY09 and MY11 and the viral typing was performed using a commercial MPCR kit. Setting: Laboratorio de Genetica Molecular-Instituto de Oncologia y Hematologia; Results: The results showed that 26 % of the evaluated sample (13/50) was positive for the presence of human papillomavirus genome. Viral typing test identified high- oncogenic risk human papillomavirus (types 16 or 18) in 38.45.% of the positives cases. Likewise, 38.45 % was low oncogenic risk (types 6, 11 or mixed infection with 6/11 human papillomavirus) and 23.07 % could not be typified with the used methodology. Conclusions: Although this result is not statistically significant, the virus latent presence highlights the need for greater medical surveillance for positive patients, especially in cases where detected 16 and 18 high-risk oncogenic human papillomavirus types, because they may have increased risk of cervical cancer.
rEsumEN OBJETIVO: Numerosos trabajos han demostrado que la infección persistente con algunos tipo... more rEsumEN OBJETIVO: Numerosos trabajos han demostrado que la infección persistente con algunos tipos de virus papiloma humano de alto riesgo incrementa el riesgo para desarrollar neoplasias intra-epiteliales cervicales de alto grado y progresión hacia el carcinoma de cérvix. En nuestro país esta patología constituye una de las principales causas de muerte por cáncer, el objetivo del presente estudio es realizar la tipificación del virus en 37 muestras de mujeres con lesiones intra-epiteliales de bajo y alto grado, que acudieron a consulta de cuello uterino del servicio de ginecología de nuestro hospital. MÉTODO: Se utilizó el sistema de hibridación reversa de INNO-LiPA HPV EXTRA Amp. RESULTADOS: En el estudio, el virus se detectó en todas las muestras. La infección con virus de alto riesgo en las lesiones intra epiteliales de bajo grado se observó en 69,57 %, siendo los tipos 16 y 51 los más prevalentes con 34,85 % cada uno, seguido de los tipos 31 y 52 con un 13,04 % cada uno. En cua...
Objective: The objective of this study was to analyze the expression of proliferative markers and... more Objective: The objective of this study was to analyze the expression of proliferative markers and p53 in keratocystic odontogenic tumor (KCOT) sporadic type and KCOT associated with nevoid basal cell carcinoma syndrome (NBCCS). Study Design and Setting: We performed a cross-sectional study. A total of 19 patients with KCOT were selected from the Oral Pathology Laboratory archives, Central University of Venezuela, from 1995 to 2005. Subjects and Methods: Twelve cases corresponded to sporadic KCOT, and seven cases were associated with NBCCS. Immunohistochemical analysis for p53, proliferating cell nuclear antigen (PCNA), and Ki-67 was performed in all 19 cases. Results: Of the seven cases associated with NBCCS, six (86%) were positive for PCNA. From the 12 sporadic cases, nine (75%) were positive for PCNA. Only one case of sporadic KCOT showed Ki-67 positivity. Five of 12 (42%) cases of sporadic KCOT were positive for p53, and only one (14%) case associated with NBCCS was positive for...
Helicobacter pylori has been implicated as the major acquired factor in the pathogenesis of chron... more Helicobacter pylori has been implicated as the major acquired factor in the pathogenesis of chronic gastritis, peptic ulcer disease, and gastric neoplasia in humans. The microorganism has been detected in dental plaque, saliva and feces. In this study, we investigated the salivary anti H. pylori immunoglubulin A (IgA) immune response in 39 dyspeptic patients and 20 healthy volunteers. Gastric antrum biopsies were taken for histological examination, urease test, culture and polymerase chain reaction (PCR). Five mililiters of unstimulated saliva were obtained from all subjects before the endoscopy. The levels of specific antibodies in saliva were determined by ELISA test. The soluble antigen material for immunoenzymatic assay was prepared by sonication of five H. pylori strains isolated from duodenal ulcer patients. H. pylori was detected in antral samples from 24/39 (62%) patients, all of whom had chronic gastritis. Symptomatic patients infected with H. pylori showed levels significa...
Revista De Obstetricia Y Ginecologia De Venezuela, Dec 1, 2013
Objetivo: Deteccion subclinica del virus de papiloma humano en muestras de pacientes diagnosticad... more Objetivo: Deteccion subclinica del virus de papiloma humano en muestras de pacientes diagnosticadas con ectropion cervical, para evaluar la prevalencia de la infeccion viral asociada a dicha condicion. Metodos: El ADN fue extraido utilizando solventes organicos (fenol/cloroformo-alcohol isoamilico). La deteccion del virus de papiloma humano se realizo mediante PCR con iniciadores genericos MY09/MY11 y para la tipificacion de las muestras positivas se utilizo un kit comercial de PCR multiple. Ambiente: Laboratorio de Genetica Molecular-Instituto de Oncologia y Hematologia. Resultados: Se obtuvo una positividad de 26 % (13/50 muestras analizadas) para la presencia de ADN del virus de papiloma humano. De las muestras positivas, 38,45 % resulto virus de papiloma humano de alto riesgo oncogenico (tipo 16 o 18), mientras que otro 38,45 % correspondio a virus de papiloma humano de bajo riesgo (tipos 6, 11 o infeccion mixta 6/11) y 23,07 % no pudo ser tipificado con la metodologia utilizada. Conclusion: Aunque este resultado no fue estadisticamente significativo, senala la necesidad de mayor seguimiento clinico de las pacientes positivas, especialmente aquellos casos correspondientes a virus de papiloma humano de alto riesgo oncogenico, ya que presentan mayor probabilidad de desarrollar cancer cervical. Objective: In this study was conducted subclinical human papillomavirus detection in samples from patients diagnosed with cervical ectropion to assess the prevalence of human papillomavirus infection associated with this condition. Methods: DNA was extracted with organic solvents (phenol /chloroform- isoamylic). Human papillomavirus detection was performed by PCR with generic primers MY09 and MY11 and the viral typing was performed using a commercial MPCR kit. Setting: Laboratorio de Genetica Molecular-Instituto de Oncologia y Hematologia; Results: The results showed that 26 % of the evaluated sample (13/50) was positive for the presence of human papillomavirus genome. Viral typing test identified high- oncogenic risk human papillomavirus (types 16 or 18) in 38.45.% of the positives cases. Likewise, 38.45 % was low oncogenic risk (types 6, 11 or mixed infection with 6/11 human papillomavirus) and 23.07 % could not be typified with the used methodology. Conclusions: Although this result is not statistically significant, the virus latent presence highlights the need for greater medical surveillance for positive patients, especially in cases where detected 16 and 18 high-risk oncogenic human papillomavirus types, because they may have increased risk of cervical cancer.
rEsumEN OBJETIVO: Numerosos trabajos han demostrado que la infección persistente con algunos tipo... more rEsumEN OBJETIVO: Numerosos trabajos han demostrado que la infección persistente con algunos tipos de virus papiloma humano de alto riesgo incrementa el riesgo para desarrollar neoplasias intra-epiteliales cervicales de alto grado y progresión hacia el carcinoma de cérvix. En nuestro país esta patología constituye una de las principales causas de muerte por cáncer, el objetivo del presente estudio es realizar la tipificación del virus en 37 muestras de mujeres con lesiones intra-epiteliales de bajo y alto grado, que acudieron a consulta de cuello uterino del servicio de ginecología de nuestro hospital. MÉTODO: Se utilizó el sistema de hibridación reversa de INNO-LiPA HPV EXTRA Amp. RESULTADOS: En el estudio, el virus se detectó en todas las muestras. La infección con virus de alto riesgo en las lesiones intra epiteliales de bajo grado se observó en 69,57 %, siendo los tipos 16 y 51 los más prevalentes con 34,85 % cada uno, seguido de los tipos 31 y 52 con un 13,04 % cada uno. En cua...
Objective: The objective of this study was to analyze the expression of proliferative markers and... more Objective: The objective of this study was to analyze the expression of proliferative markers and p53 in keratocystic odontogenic tumor (KCOT) sporadic type and KCOT associated with nevoid basal cell carcinoma syndrome (NBCCS). Study Design and Setting: We performed a cross-sectional study. A total of 19 patients with KCOT were selected from the Oral Pathology Laboratory archives, Central University of Venezuela, from 1995 to 2005. Subjects and Methods: Twelve cases corresponded to sporadic KCOT, and seven cases were associated with NBCCS. Immunohistochemical analysis for p53, proliferating cell nuclear antigen (PCNA), and Ki-67 was performed in all 19 cases. Results: Of the seven cases associated with NBCCS, six (86%) were positive for PCNA. From the 12 sporadic cases, nine (75%) were positive for PCNA. Only one case of sporadic KCOT showed Ki-67 positivity. Five of 12 (42%) cases of sporadic KCOT were positive for p53, and only one (14%) case associated with NBCCS was positive for...
Helicobacter pylori has been implicated as the major acquired factor in the pathogenesis of chron... more Helicobacter pylori has been implicated as the major acquired factor in the pathogenesis of chronic gastritis, peptic ulcer disease, and gastric neoplasia in humans. The microorganism has been detected in dental plaque, saliva and feces. In this study, we investigated the salivary anti H. pylori immunoglubulin A (IgA) immune response in 39 dyspeptic patients and 20 healthy volunteers. Gastric antrum biopsies were taken for histological examination, urease test, culture and polymerase chain reaction (PCR). Five mililiters of unstimulated saliva were obtained from all subjects before the endoscopy. The levels of specific antibodies in saliva were determined by ELISA test. The soluble antigen material for immunoenzymatic assay was prepared by sonication of five H. pylori strains isolated from duodenal ulcer patients. H. pylori was detected in antral samples from 24/39 (62%) patients, all of whom had chronic gastritis. Symptomatic patients infected with H. pylori showed levels significa...
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