Diarrhea and digestive disorders represent one of the leading causes of mortality in calves durin... more Diarrhea and digestive disorders represent one of the leading causes of mortality in calves during the first days of life. This study aimed to establish the level of antibiotic resistance of the flora identified in calves with digestive problems and also in their environment. Bălțată Românească calves aged up to three weeks, showing an increased morbidity/mortality from a farm in Brașov county, as well as their habitat were sampled. Eight antimicrobials used in ruminants and on this specific farm (penicillin, streptomycin, amoxicillin, gentamicin, oxytetracycline, tulathromycin, cefaclor, trimethoprim/ sulfamethoxazole) were tested for their efficacy against the bacterial isolates. The samples were subjected to standard microbiological examinations; subsequently, strains such as Escherichia fergusoni, Escherichia hermannii, Shigella dysenteriae, Proteus penneri, Morganella morganii ssp. siboni, Escherichia coli, Escherichia vulneris, with potentially high pathogenicity were identified. The highest antibacterial efficacy was observed with gentamicin. The MAR (multiple antibiotic resistance) index exceeded the value of 0.2 for each of the tested strains, indicating a high level of antibiotic resistance of the isolated bacterial population, thus supporting the inducive role of isolated species in the clinical episodes.
Antibiotic resistance of Staphylococcus is increasing worldwide and is becoming a global concern.... more Antibiotic resistance of Staphylococcus is increasing worldwide and is becoming a global concern. The present report exposes the study of the antibiotic susceptibility in isolates of Staphylococcus spp obtained from farm pigs. The samples were collected from healthy pigs, from different farms, in the period between 2019-2020. Forty strains of Staphylococcus were isolated from the anterior nares of individual pigs, and from the skin behind the ears of the animals. These isolates were tested against 12 different antimicrobial agents using the Kirby-Bauer disk-diffusion method. The antibiotic susceptibility profile of different bacteria isolates was studied to detect MAR, MDR. Multiple antibiotic resistance (MAR) indices were determined and it was above 0.2 in 24 of the total of 40 bacteria studied. The highest MAR index was detected in Staphylococcus lentus (0.5) and Staphylococcus xylosus (0.41) and 26 (65%) strains was MDR. In conclusion, the high levels of antibiotic resistance generally correlates with high antibiotic usage and antibiotics used are a risk to humans with occupational contact with livestock antibiotics.
Diarrhea and digestive disorders represent one of the leading causes of mortality in calves durin... more Diarrhea and digestive disorders represent one of the leading causes of mortality in calves during the first days of life. This study aimed to establish the level of antibiotic resistance of the flora identified in calves with digestive problems and also in their environment. Bălțată Românească calves aged up to three weeks, showing an increased morbidity/mortality from a farm in Brașov county, as well as their habitat were sampled. Eight antimicrobials used in ruminants and on this specific farm (penicillin, streptomycin, amoxicillin, gentamicin, oxytetracycline, tulathromycin, cefaclor, trimethoprim/ sulfamethoxazole) were tested for their efficacy against the bacterial isolates. The samples were subjected to standard microbiological examinations; subsequently, strains such as Escherichia fergusoni, Escherichia hermannii, Shigella dysenteriae, Proteus penneri, Morganella morganii ssp. siboni, Escherichia coli, Escherichia vulneris, with potentially high pathogenicity were identified. The highest antibacterial efficacy was observed with gentamicin. The MAR (multiple antibiotic resistance) index exceeded the value of 0.2 for each of the tested strains, indicating a high level of antibiotic resistance of the isolated bacterial population, thus supporting the inducive role of isolated species in the clinical episodes.
Antibiotic resistance of Staphylococcus is increasing worldwide and is becoming a global concern.... more Antibiotic resistance of Staphylococcus is increasing worldwide and is becoming a global concern. The present report exposes the study of the antibiotic susceptibility in isolates of Staphylococcus spp obtained from farm pigs. The samples were collected from healthy pigs, from different farms, in the period between 2019-2020. Forty strains of Staphylococcus were isolated from the anterior nares of individual pigs, and from the skin behind the ears of the animals. These isolates were tested against 12 different antimicrobial agents using the Kirby-Bauer disk-diffusion method. The antibiotic susceptibility profile of different bacteria isolates was studied to detect MAR, MDR. Multiple antibiotic resistance (MAR) indices were determined and it was above 0.2 in 24 of the total of 40 bacteria studied. The highest MAR index was detected in Staphylococcus lentus (0.5) and Staphylococcus xylosus (0.41) and 26 (65%) strains was MDR. In conclusion, the high levels of antibiotic resistance generally correlates with high antibiotic usage and antibiotics used are a risk to humans with occupational contact with livestock antibiotics.
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