Journal of Oral Pathology and Medicine, Jul 31, 1997
... Page 3. 336 LUOMANEN et al. binding to Tn, as characterized earlier in detail by HOWEEDY et a... more ... Page 3. 336 LUOMANEN et al. binding to Tn, as characterized earlier in detail by HOWEEDY et al (40), in phosphate buffered saline (PBS) at dilu-tions of 1:100 at room temperature for 30 min. ... VAIDYA MM. BORGES AM. PRADHAN SA. RAJPAL RM, BHISEY AN. ...
Nimiösivulla myös: Department of Cariology and Department of Pathology, University of Helsinki, F... more Nimiösivulla myös: Department of Cariology and Department of Pathology, University of Helsinki, Finland. - Väitösk. lisäksi 1 irtol. - Also publ. in Proceedings of the Finnish Dental Society, 1987, vol. 83, suppl. XII. - Tiivistelmä ja 5 erip. Diss. -- Helsingin yliopisto.
Journal of Oral Pathology and Medicine, Jul 31, 1997
... Page 3. 336 LUOMANEN et al. binding to Tn, as characterized earlier in detail by HOWEEDY et a... more ... Page 3. 336 LUOMANEN et al. binding to Tn, as characterized earlier in detail by HOWEEDY et al (40), in phosphate buffered saline (PBS) at dilu-tions of 1:100 at room temperature for 30 min. ... VAIDYA MM. BORGES AM. PRADHAN SA. RAJPAL RM, BHISEY AN. ...
Nimiösivulla myös: Department of Cariology and Department of Pathology, University of Helsinki, F... more Nimiösivulla myös: Department of Cariology and Department of Pathology, University of Helsinki, Finland. - Väitösk. lisäksi 1 irtol. - Also publ. in Proceedings of the Finnish Dental Society, 1987, vol. 83, suppl. XII. - Tiivistelmä ja 5 erip. Diss. -- Helsingin yliopisto.
Proceedings of the Finnish Dental Society. Suomen Hammaslääkäriseuran toimituksia
Fifty-six patients with 86 benign oral soft-tissue lesions were treated with CO2 laser surgery du... more Fifty-six patients with 86 benign oral soft-tissue lesions were treated with CO2 laser surgery during a period of 48 months. The lesions removed included hyperplasias, papillomas, haemangiomas, mucoceles, lichen planus, fibromas, condylomas and lingual frenula. All clinical diagnoses except that of lingual frenulum were verified histopathologically. The lesions were excised using the laser to obtain biopsy specimens. However, in some cases it was felt more appropriate to excise biopsy material from the most characteristic part of the lesion first, then to destroy the rest of the lesion. Treatment was undertaken in one session under local anaesthesia without premedication. No patient received antibiotics or anti-inflammatory drugs after operation. The lesions were operated on freehand using a handpiece and laser power ranging from 6 to 10 W in continuous mode. The beam in focus was 0.2 mm in diameter, which allowed fine dissection. There was no bleeding from the laser wounds, and no ...
Proceedings of the Finnish Dental Society. Suomen Hammaslääkäriseuran toimituksia
Fifty-six patients with 86 benign oral soft-tissue lesions were treated with CO2 laser surgery du... more Fifty-six patients with 86 benign oral soft-tissue lesions were treated with CO2 laser surgery during a period of 48 months. The lesions removed included hyperplasias, papillomas, haemangiomas, mucoceles, lichen planus, fibromas, condylomas and lingual frenula. All clinical diagnoses except that of lingual frenulum were verified histopathologically. The lesions were excised using the laser to obtain biopsy specimens. However, in some cases it was felt more appropriate to excise biopsy material from the most characteristic part of the lesion first, then to destroy the rest of the lesion. Treatment was undertaken in one session under local anaesthesia without premedication. No patient received antibiotics or anti-inflammatory drugs after operation. The lesions were operated on freehand using a handpiece and laser power ranging from 6 to 10 W in continuous mode. The beam in focus was 0.2 mm in diameter, which allowed fine dissection. There was no bleeding from the laser wounds, and no ...
Differences in cytokeratin expression of clinically normal buccal mucosa were studied in 50 healt... more Differences in cytokeratin expression of clinically normal buccal mucosa were studied in 50 healthy women by indirect immunofluorescence staining with monoclonal antibodies. The subjects were divided into four groups: control group (N = 18), smokers (N = 8), oral contraceptive users (N = 8) and smokers receiving oral contraceptives (N = 16). Our findings indicate that cytokeratin expression in noncornified stratified epithelium is not influenced by smoking or oestradiol/progesterone treatment. Only cytokeratin No. 19 showed variable patterns of expression but the differences could not be ascribed to smoking or contraceptives. Cytokeratin No. 19 gave a positive reaction in the basal and suprabasal layers in 34 subjects (68%). In 9 (18%) specimens, the staining was positive in the basal cells and showed a positive heterogeneous cytoplasmic reaction in the suprabasal cells. Interestingly, cytokeratin No. 7 was recognized in all epithelial cells except the basal cells. Our results suggest that changes in the serum oestradiol levels do not affect the cytokeratin pattern in buccal mucosa.
Extracellular matrix components laminin, Type IV collagen, Type III collagen, and fibronectin, of... more Extracellular matrix components laminin, Type IV collagen, Type III collagen, and fibronectin, of laser-treated rat tongue mucosa were studied over a healing period of 28 days by using immunohistochemical and electron microscopic techniques. Their distribution and amount in laser wounds was compared with that in scalpel incisions and normal tongue mucosa. Laser treatment caused an extensive destruction of both epithelial and stromal cells but left much of the connective tissue matrix intact. Basement membranes, in particular, appeared to resist laser irradiation. During the study period the laser-induced lesions healed through reparative synthesis of matrix proteins which led to filling of the tissue defects. The regenerative processes with concomitant re-epithelialization took place more slowly in laser-treated than in scalpel incision wounds. The relative resistance of the matrix proteins against laser irradiation and the slow removal and replacement of the residual matrix is suggested to account at least partially for the lack of scarring and contraction frequently observed in laser-treated areas.
The distribution of the extracellular matrix glycoprotein, tenascin, was studied in normal mucosa... more The distribution of the extracellular matrix glycoprotein, tenascin, was studied in normal mucosa and during healing of scalpel incised or excised and CO2 laser-wounded rat tongue dorsal mucosa in 51 male Sprague-Dawley rats over a period of 21 days. A polyclonal antibody specific for tenascin was applied in indirect immunofluorescence microscopy. In normal mucosa tenascin was sparsely distributed in a discontinuous manner at the tips of the connective tissue papillae in association with the basement membrane (BM) and in the walls of the capillaries. In all the healing wounds there was a marked increase in the distribution of tenascin, particularly close to the BMs at the wound edges beneath the proliferating and migrating epithelium, and later on during healing in the regenerating connective tissue (CT) area. This expression subsided later on during healing. Laser surgery did not alter the ability of fibroblasts to synthesize tenascin. The transient expression of tenascin in the BMs and CT of the healing wounds suggests that this protein could play an important role in providing ideal conditions for cell movement, and in the deposition and organization of other extracellular matrix (ECM) glycoproteins during tissue repair.
The healing process of laser- and scalpel-incision wounds of rat tongue mucosa was investigated b... more The healing process of laser- and scalpel-incision wounds of rat tongue mucosa was investigated by the indirect immunofluorescence technique using a monoclonal cytokeratin antibody (PKK2) binding to low molecular weight keratins of basal keratinocytes, two antibodies reacting with suprabasal keratins and a monoclonal antibody reacting with desmosomes. The wounds were made parallel on both sides of the tongue in thirty animals. In normal tongue only the basal cell layer and the cells at the base of the rete ridges showed PKK2-positivity. In both types of wounds at the same healing phase the whole proliferative cell layer showed a bright PKK2-positivity. The cell proliferation appeared to be confined to all cells of the mucosa. The disturbance in the keratin expression of the healing mucosa could also be revealed with another monoclonal (58CH8) antibody, which reacted only with the suprabasal cells in normal tongue mucosa but lacked reactivity with the healing keratinocytes, whereas polyclonal antibodies to callus keratins showed a bright reactivity also in the healing keratinocytes. Monoclonal antibodies to desmoplakins indicated that the keratinocytes in both types of wounds had normal desmosomal cell-to-cell junctions. As the healing process advanced, the PKK2-positivity regressed to that seen in normal tongue mucosa.
Differences in the expression of cytokeratins (CK) in specimens obtained from snuff-affected oral... more Differences in the expression of cytokeratins (CK) in specimens obtained from snuff-affected oral epithelium of the maxillary vestibular sulcus and clinically normal sulcular epithelium were studied by indirect immunofluorescence staining with a panel of monoclonal antibodies (MAbs). CK 14, a marker of stratified squamous epithelium was not seen expressed in 3/11 of the snuff user's specimens. Terminal differentiation markers, typical of cornified epithelia (CK 1, 9, 10 and 11), were detected suprabasally in the snuff user's keratosis but not in the normal control epithelium. The use of snuff seemed to change the CK staining pattern of the mucosa so that it resembles more that of a cornified type of epithelium. Simple epithelial-type CK were included in the study in order to establish the CK profile of the snuff-induced keratosis, for comparison with normal and dysplastic lesions. MAb to CK 7 and 19 showed reactivity in the basal cells and suprabasally whereas the monospecific MAb anti-CK 7 showed suprabasal staining both in the control and affected epithelia. By using MAbs, we found no immunoreactivity against CK 18 either in normal or affected epithelia, whereas we found suprabasal reaction (5/11) against CK 8 in the snuff user's epithelia. The two MAbs demonstrating the expression of CK 19, normally confined to the basal cells of the stratified squamous epithelium, showed variable patterns of expression both in basal cells and suprabasally in the snuff lesions. The results show that use of oral snuff causes some alterations in the CK expression pattern of the affected epithelium. Whether the alterations are indicative of a premalignant change is, however, uncertain. The results encourage further studies on the subject.
... Page 3. 336 LUOMANEN et al. binding to Tn, as characterized earlier in detail by HOWEEDY et a... more ... Page 3. 336 LUOMANEN et al. binding to Tn, as characterized earlier in detail by HOWEEDY et al (40), in phosphate buffered saline (PBS) at dilu-tions of 1:100 at room temperature for 30 min. ... VAIDYA MM. BORGES AM. PRADHAN SA. RAJPAL RM, BHISEY AN. ...
Journal of Oral Pathology and Medicine, Jul 31, 1997
... Page 3. 336 LUOMANEN et al. binding to Tn, as characterized earlier in detail by HOWEEDY et a... more ... Page 3. 336 LUOMANEN et al. binding to Tn, as characterized earlier in detail by HOWEEDY et al (40), in phosphate buffered saline (PBS) at dilu-tions of 1:100 at room temperature for 30 min. ... VAIDYA MM. BORGES AM. PRADHAN SA. RAJPAL RM, BHISEY AN. ...
Nimiösivulla myös: Department of Cariology and Department of Pathology, University of Helsinki, F... more Nimiösivulla myös: Department of Cariology and Department of Pathology, University of Helsinki, Finland. - Väitösk. lisäksi 1 irtol. - Also publ. in Proceedings of the Finnish Dental Society, 1987, vol. 83, suppl. XII. - Tiivistelmä ja 5 erip. Diss. -- Helsingin yliopisto.
Journal of Oral Pathology and Medicine, Jul 31, 1997
... Page 3. 336 LUOMANEN et al. binding to Tn, as characterized earlier in detail by HOWEEDY et a... more ... Page 3. 336 LUOMANEN et al. binding to Tn, as characterized earlier in detail by HOWEEDY et al (40), in phosphate buffered saline (PBS) at dilu-tions of 1:100 at room temperature for 30 min. ... VAIDYA MM. BORGES AM. PRADHAN SA. RAJPAL RM, BHISEY AN. ...
Nimiösivulla myös: Department of Cariology and Department of Pathology, University of Helsinki, F... more Nimiösivulla myös: Department of Cariology and Department of Pathology, University of Helsinki, Finland. - Väitösk. lisäksi 1 irtol. - Also publ. in Proceedings of the Finnish Dental Society, 1987, vol. 83, suppl. XII. - Tiivistelmä ja 5 erip. Diss. -- Helsingin yliopisto.
Proceedings of the Finnish Dental Society. Suomen Hammaslääkäriseuran toimituksia
Fifty-six patients with 86 benign oral soft-tissue lesions were treated with CO2 laser surgery du... more Fifty-six patients with 86 benign oral soft-tissue lesions were treated with CO2 laser surgery during a period of 48 months. The lesions removed included hyperplasias, papillomas, haemangiomas, mucoceles, lichen planus, fibromas, condylomas and lingual frenula. All clinical diagnoses except that of lingual frenulum were verified histopathologically. The lesions were excised using the laser to obtain biopsy specimens. However, in some cases it was felt more appropriate to excise biopsy material from the most characteristic part of the lesion first, then to destroy the rest of the lesion. Treatment was undertaken in one session under local anaesthesia without premedication. No patient received antibiotics or anti-inflammatory drugs after operation. The lesions were operated on freehand using a handpiece and laser power ranging from 6 to 10 W in continuous mode. The beam in focus was 0.2 mm in diameter, which allowed fine dissection. There was no bleeding from the laser wounds, and no ...
Proceedings of the Finnish Dental Society. Suomen Hammaslääkäriseuran toimituksia
Fifty-six patients with 86 benign oral soft-tissue lesions were treated with CO2 laser surgery du... more Fifty-six patients with 86 benign oral soft-tissue lesions were treated with CO2 laser surgery during a period of 48 months. The lesions removed included hyperplasias, papillomas, haemangiomas, mucoceles, lichen planus, fibromas, condylomas and lingual frenula. All clinical diagnoses except that of lingual frenulum were verified histopathologically. The lesions were excised using the laser to obtain biopsy specimens. However, in some cases it was felt more appropriate to excise biopsy material from the most characteristic part of the lesion first, then to destroy the rest of the lesion. Treatment was undertaken in one session under local anaesthesia without premedication. No patient received antibiotics or anti-inflammatory drugs after operation. The lesions were operated on freehand using a handpiece and laser power ranging from 6 to 10 W in continuous mode. The beam in focus was 0.2 mm in diameter, which allowed fine dissection. There was no bleeding from the laser wounds, and no ...
Differences in cytokeratin expression of clinically normal buccal mucosa were studied in 50 healt... more Differences in cytokeratin expression of clinically normal buccal mucosa were studied in 50 healthy women by indirect immunofluorescence staining with monoclonal antibodies. The subjects were divided into four groups: control group (N = 18), smokers (N = 8), oral contraceptive users (N = 8) and smokers receiving oral contraceptives (N = 16). Our findings indicate that cytokeratin expression in noncornified stratified epithelium is not influenced by smoking or oestradiol/progesterone treatment. Only cytokeratin No. 19 showed variable patterns of expression but the differences could not be ascribed to smoking or contraceptives. Cytokeratin No. 19 gave a positive reaction in the basal and suprabasal layers in 34 subjects (68%). In 9 (18%) specimens, the staining was positive in the basal cells and showed a positive heterogeneous cytoplasmic reaction in the suprabasal cells. Interestingly, cytokeratin No. 7 was recognized in all epithelial cells except the basal cells. Our results suggest that changes in the serum oestradiol levels do not affect the cytokeratin pattern in buccal mucosa.
Extracellular matrix components laminin, Type IV collagen, Type III collagen, and fibronectin, of... more Extracellular matrix components laminin, Type IV collagen, Type III collagen, and fibronectin, of laser-treated rat tongue mucosa were studied over a healing period of 28 days by using immunohistochemical and electron microscopic techniques. Their distribution and amount in laser wounds was compared with that in scalpel incisions and normal tongue mucosa. Laser treatment caused an extensive destruction of both epithelial and stromal cells but left much of the connective tissue matrix intact. Basement membranes, in particular, appeared to resist laser irradiation. During the study period the laser-induced lesions healed through reparative synthesis of matrix proteins which led to filling of the tissue defects. The regenerative processes with concomitant re-epithelialization took place more slowly in laser-treated than in scalpel incision wounds. The relative resistance of the matrix proteins against laser irradiation and the slow removal and replacement of the residual matrix is suggested to account at least partially for the lack of scarring and contraction frequently observed in laser-treated areas.
The distribution of the extracellular matrix glycoprotein, tenascin, was studied in normal mucosa... more The distribution of the extracellular matrix glycoprotein, tenascin, was studied in normal mucosa and during healing of scalpel incised or excised and CO2 laser-wounded rat tongue dorsal mucosa in 51 male Sprague-Dawley rats over a period of 21 days. A polyclonal antibody specific for tenascin was applied in indirect immunofluorescence microscopy. In normal mucosa tenascin was sparsely distributed in a discontinuous manner at the tips of the connective tissue papillae in association with the basement membrane (BM) and in the walls of the capillaries. In all the healing wounds there was a marked increase in the distribution of tenascin, particularly close to the BMs at the wound edges beneath the proliferating and migrating epithelium, and later on during healing in the regenerating connective tissue (CT) area. This expression subsided later on during healing. Laser surgery did not alter the ability of fibroblasts to synthesize tenascin. The transient expression of tenascin in the BMs and CT of the healing wounds suggests that this protein could play an important role in providing ideal conditions for cell movement, and in the deposition and organization of other extracellular matrix (ECM) glycoproteins during tissue repair.
The healing process of laser- and scalpel-incision wounds of rat tongue mucosa was investigated b... more The healing process of laser- and scalpel-incision wounds of rat tongue mucosa was investigated by the indirect immunofluorescence technique using a monoclonal cytokeratin antibody (PKK2) binding to low molecular weight keratins of basal keratinocytes, two antibodies reacting with suprabasal keratins and a monoclonal antibody reacting with desmosomes. The wounds were made parallel on both sides of the tongue in thirty animals. In normal tongue only the basal cell layer and the cells at the base of the rete ridges showed PKK2-positivity. In both types of wounds at the same healing phase the whole proliferative cell layer showed a bright PKK2-positivity. The cell proliferation appeared to be confined to all cells of the mucosa. The disturbance in the keratin expression of the healing mucosa could also be revealed with another monoclonal (58CH8) antibody, which reacted only with the suprabasal cells in normal tongue mucosa but lacked reactivity with the healing keratinocytes, whereas polyclonal antibodies to callus keratins showed a bright reactivity also in the healing keratinocytes. Monoclonal antibodies to desmoplakins indicated that the keratinocytes in both types of wounds had normal desmosomal cell-to-cell junctions. As the healing process advanced, the PKK2-positivity regressed to that seen in normal tongue mucosa.
Differences in the expression of cytokeratins (CK) in specimens obtained from snuff-affected oral... more Differences in the expression of cytokeratins (CK) in specimens obtained from snuff-affected oral epithelium of the maxillary vestibular sulcus and clinically normal sulcular epithelium were studied by indirect immunofluorescence staining with a panel of monoclonal antibodies (MAbs). CK 14, a marker of stratified squamous epithelium was not seen expressed in 3/11 of the snuff user's specimens. Terminal differentiation markers, typical of cornified epithelia (CK 1, 9, 10 and 11), were detected suprabasally in the snuff user's keratosis but not in the normal control epithelium. The use of snuff seemed to change the CK staining pattern of the mucosa so that it resembles more that of a cornified type of epithelium. Simple epithelial-type CK were included in the study in order to establish the CK profile of the snuff-induced keratosis, for comparison with normal and dysplastic lesions. MAb to CK 7 and 19 showed reactivity in the basal cells and suprabasally whereas the monospecific MAb anti-CK 7 showed suprabasal staining both in the control and affected epithelia. By using MAbs, we found no immunoreactivity against CK 18 either in normal or affected epithelia, whereas we found suprabasal reaction (5/11) against CK 8 in the snuff user's epithelia. The two MAbs demonstrating the expression of CK 19, normally confined to the basal cells of the stratified squamous epithelium, showed variable patterns of expression both in basal cells and suprabasally in the snuff lesions. The results show that use of oral snuff causes some alterations in the CK expression pattern of the affected epithelium. Whether the alterations are indicative of a premalignant change is, however, uncertain. The results encourage further studies on the subject.
... Page 3. 336 LUOMANEN et al. binding to Tn, as characterized earlier in detail by HOWEEDY et a... more ... Page 3. 336 LUOMANEN et al. binding to Tn, as characterized earlier in detail by HOWEEDY et al (40), in phosphate buffered saline (PBS) at dilu-tions of 1:100 at room temperature for 30 min. ... VAIDYA MM. BORGES AM. PRADHAN SA. RAJPAL RM, BHISEY AN. ...
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