<p>*p<0.05 RS218 vs. DH5−α; † p<0.05 SCB34 vs. DH5−α; ‡ p<0.05 SCB34 vs. RS218. Mo... more <p>*p<0.05 RS218 vs. DH5−α; † p<0.05 SCB34 vs. DH5−α; ‡ p<0.05 SCB34 vs. RS218. Model−calculated mean of cumulative log<sub>e</sub> (CFU+1) values over time are shown. Error bars reflect 95% Bayesian confidence intervals. Points were jittered to improve clarity (combined data from three separate experiments).</p
<p><b>1A.</b> Animals were inoculated orally and survival post-inoculation is s... more <p><b>1A.</b> Animals were inoculated orally and survival post-inoculation is shown (data from three separate experiments, n = 10 animals per group, per experiment). <i>*p<0</i>.<i>05 SCB34 vs</i>. <i>DH5α</i>, <i>**p<0</i>.<i>03 RS218 vs</i>. <i>DH5α</i>. <b>1B.</b> Survival after intraperitoneal inoculation (data from two separate experiments, n = 10 animals per group, per experiment). <i>‡p <</i> .<i>001 RS218 vs</i>. <i>SCB34 or RS218 vs</i>. <i>DH5α</i>.</p
<p>The nonpathogenic laboratory strain DH5α was used as control. SCB34 demonstrated the hig... more <p>The nonpathogenic laboratory strain DH5α was used as control. SCB34 demonstrated the highest invasion ability among the three strains. *SCB34 <i>vs</i>. RS218 (p≤0.03); **SCB34 <i>vs</i>. and DH5α (p<0.001); and ***RS218 <i>vs</i>. DH5α (p<0.01).</p
<p>The Venn diagram shows genes that are common or unique genes in each isolate. The unique... more <p>The Venn diagram shows genes that are common or unique genes in each isolate. The unique proteins have been determined by comparison between the 3 strains.</p
<p>SCB34 and RS218 are highlighted by the arrows, among 33 additional representative <i&... more <p>SCB34 and RS218 are highlighted by the arrows, among 33 additional representative <i>E</i>. <i>coli</i> strains. The dotted-line boxes highlight the clade that includes SCB34, and the names of the two additional <i>E</i>. <i>coli</i> strains with the most similar protein-coding sequences within the clade. A hierarchical clustering of the genes found in all genomes is found at the left of the heat map, while a tree based on hierarchical clustering of genomes is shown at the top of the heat map.</p
<p>Growth curves performed in (4A) LB media and (4B) tissue culture media (TCM) by plate co... more <p>Growth curves performed in (4A) LB media and (4B) tissue culture media (TCM) by plate counts. Each plot shows mean CFU values in triplicate samples per time point of each individual clinical isolates. Error bars represent one standard deviation.</p
Escherichia coli is the leading cause of Gram-negative neonatal septicemia in the United States. ... more Escherichia coli is the leading cause of Gram-negative neonatal septicemia in the United States. Invasion and passage across the neonatal gut after ingestion of maternal E. coli strains produce bacteremia. In this study, we compared the virulence properties of the neonatal E. coli bacteremia clinical isolate SCB34 with the archetypal neonatal E. coli meningitis strain RS218. Whole-genome sequencing data was used to compare the protein coding sequences among these clinical isolates and 33 other representative E. coli strains. Oral inoculation of newborn animals with either strain produced septicemia, whereas intraperitoneal injection caused septicemia only in pups infected with RS218 but not in those injected with SCB34. In addition to being virulent only through the oral route, SCB34 demonstrated significantly greater invasion and transcytosis of polarized intestinal epithelial cells in vitro as compared to RS218. Protein coding sequences comparisons highlighted the presence of know...
<p>*p<0.05 RS218 vs. DH5−α; † p<0.05 SCB34 vs. DH5−α; ‡ p<0.05 SCB34 vs. RS218. Mo... more <p>*p<0.05 RS218 vs. DH5−α; † p<0.05 SCB34 vs. DH5−α; ‡ p<0.05 SCB34 vs. RS218. Model−calculated mean of cumulative log<sub>e</sub> (CFU+1) values over time are shown. Error bars reflect 95% Bayesian confidence intervals. Points were jittered to improve clarity (combined data from three separate experiments).</p
<p><b>1A.</b> Animals were inoculated orally and survival post-inoculation is s... more <p><b>1A.</b> Animals were inoculated orally and survival post-inoculation is shown (data from three separate experiments, n = 10 animals per group, per experiment). <i>*p<0</i>.<i>05 SCB34 vs</i>. <i>DH5α</i>, <i>**p<0</i>.<i>03 RS218 vs</i>. <i>DH5α</i>. <b>1B.</b> Survival after intraperitoneal inoculation (data from two separate experiments, n = 10 animals per group, per experiment). <i>‡p <</i> .<i>001 RS218 vs</i>. <i>SCB34 or RS218 vs</i>. <i>DH5α</i>.</p
<p>The nonpathogenic laboratory strain DH5α was used as control. SCB34 demonstrated the hig... more <p>The nonpathogenic laboratory strain DH5α was used as control. SCB34 demonstrated the highest invasion ability among the three strains. *SCB34 <i>vs</i>. RS218 (p≤0.03); **SCB34 <i>vs</i>. and DH5α (p<0.001); and ***RS218 <i>vs</i>. DH5α (p<0.01).</p
<p>The Venn diagram shows genes that are common or unique genes in each isolate. The unique... more <p>The Venn diagram shows genes that are common or unique genes in each isolate. The unique proteins have been determined by comparison between the 3 strains.</p
<p>SCB34 and RS218 are highlighted by the arrows, among 33 additional representative <i&... more <p>SCB34 and RS218 are highlighted by the arrows, among 33 additional representative <i>E</i>. <i>coli</i> strains. The dotted-line boxes highlight the clade that includes SCB34, and the names of the two additional <i>E</i>. <i>coli</i> strains with the most similar protein-coding sequences within the clade. A hierarchical clustering of the genes found in all genomes is found at the left of the heat map, while a tree based on hierarchical clustering of genomes is shown at the top of the heat map.</p
<p>Growth curves performed in (4A) LB media and (4B) tissue culture media (TCM) by plate co... more <p>Growth curves performed in (4A) LB media and (4B) tissue culture media (TCM) by plate counts. Each plot shows mean CFU values in triplicate samples per time point of each individual clinical isolates. Error bars represent one standard deviation.</p
Escherichia coli is the leading cause of Gram-negative neonatal septicemia in the United States. ... more Escherichia coli is the leading cause of Gram-negative neonatal septicemia in the United States. Invasion and passage across the neonatal gut after ingestion of maternal E. coli strains produce bacteremia. In this study, we compared the virulence properties of the neonatal E. coli bacteremia clinical isolate SCB34 with the archetypal neonatal E. coli meningitis strain RS218. Whole-genome sequencing data was used to compare the protein coding sequences among these clinical isolates and 33 other representative E. coli strains. Oral inoculation of newborn animals with either strain produced septicemia, whereas intraperitoneal injection caused septicemia only in pups infected with RS218 but not in those injected with SCB34. In addition to being virulent only through the oral route, SCB34 demonstrated significantly greater invasion and transcytosis of polarized intestinal epithelial cells in vitro as compared to RS218. Protein coding sequences comparisons highlighted the presence of know...
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