The haemagglutination-inhibiting activity of human serum towards Sindbis virus is linked to very ... more The haemagglutination-inhibiting activity of human serum towards Sindbis virus is linked to very low and low density lipoprotein classes, in particular to their lipid component. To investigate the specific role played in this inhibition by carbohydrates contained in the molecule, inhibitor separation was carried out by Concanavalin A-Sepharose affinity chromatography. A different behaviour among the inhibiting molecules in relation to the carbohydrate content was observed. The importance of mannose for the biological activity of the inhibitor is discussed.
Research was carried out in order to verify if some components of human serum, which are capable ... more Research was carried out in order to verify if some components of human serum, which are capable of inhibiting the hemagglutination by Sendai virus, are active even towards the hemolysis by the same virus. It was ascertained that both orosomucoid and low-density lipoproteins obtained by floatation had no action on the hemolysis by Sendai virus while lipoproteins precipitated with dextrane sulphate showed a certain inhibiting effect. This action however is not due to lipoproteins but is probably related to some substances used in the method of precipitation.
A study has been made to clarify the importance of some carbohydrate components of goose red bloo... more A study has been made to clarify the importance of some carbohydrate components of goose red blood cell and BHK 21 cell membrane receptors for vesicular stomatitis virus (VSV). Experiments have been carried out by inducing enzymatic modifications on the surface of intact cells and following the interaction between VSV and treated cells. Results obtained indicate that the removal of mannose, galactose and fucose from cell membranes reduces the attachment of the virus to cell receptors.
Abstract: Chlamydia pneumoniae, a pathogenic bacteria responsible for respiratory tract infection... more Abstract: Chlamydia pneumoniae, a pathogenic bacteria responsible for respiratory tract infections, is known as the most implicated infectious agent in atherosclerotic cardiovascular diseases (CVDs). Accumulating evidence suggests that C. pneumoniae-induced oxidative stress may play a critical role in the pathogenesis of CVDs. Indeed, the overproduction of reactive oxygen species (ROS) within macrophages, endothelial cells, platelets and vascular smooth muscle cells (VSMCs) after C. pneumoniae exposure, has been shown to cause low density lipoprotein oxidation, foam cell formation, endothelial dysfunction, platelet adhesion and aggregation, and VSMC proliferation and migration, all responsible for the typical pathological changes of atherosclerotic plaque. The aim of this review is to improve our insight into C. pneumoniae-induced oxidative stress in order to suggest potential strategies for CVD prevention. Several antioxidants, acting on multi-enzymatic targets related to ROS produ...
The relationship between Con A-receptor mobility and Con A-induced agglutination of Novikoff hepa... more The relationship between Con A-receptor mobility and Con A-induced agglutination of Novikoff hepatoma and normal rat liver cells was investigated. Novikoff cells, incubated with fluorescein-labelled Con A at 3 °C displayed uniform, ring-like surface fluorescence. Increasing the temperature of the cells to 37 °C caused capping of Con A receptors in approximately 65% of the cells, a phenomenon that could be prevented by prefixing the cells with glutaraldehyde. In spite of these variations in Con A-receptor distribution, Con A-induced agglutination was remarkably constant over a temperature range from 3 to 37 °C. In contrast to Novikoff cells, normal hepatocytes displayed a uniform, ringlike surface fluorescence at both 3 and 37 °C. No capping was observed. However, hepatocytes, similar to Novikoff cells, were agglutinable by low concentrations of Con A. These findings indicate that, in this model system, Con A-induced cytoagglutination is not dependent upon long-range lateral mobility...
The haemagglutination-inhibiting activity of human serum towards Sindbis virus is linked to very ... more The haemagglutination-inhibiting activity of human serum towards Sindbis virus is linked to very low and low density lipoprotein classes, in particular to their lipid component. To investigate the specific role played in this inhibition by carbohydrates contained in the molecule, inhibitor separation was carried out by Concanavalin A-Sepharose affinity chromatography. A different behaviour among the inhibiting molecules in relation to the carbohydrate content was observed. The importance of mannose for the biological activity of the inhibitor is discussed.
Research was carried out in order to verify if some components of human serum, which are capable ... more Research was carried out in order to verify if some components of human serum, which are capable of inhibiting the hemagglutination by Sendai virus, are active even towards the hemolysis by the same virus. It was ascertained that both orosomucoid and low-density lipoproteins obtained by floatation had no action on the hemolysis by Sendai virus while lipoproteins precipitated with dextrane sulphate showed a certain inhibiting effect. This action however is not due to lipoproteins but is probably related to some substances used in the method of precipitation.
A study has been made to clarify the importance of some carbohydrate components of goose red bloo... more A study has been made to clarify the importance of some carbohydrate components of goose red blood cell and BHK 21 cell membrane receptors for vesicular stomatitis virus (VSV). Experiments have been carried out by inducing enzymatic modifications on the surface of intact cells and following the interaction between VSV and treated cells. Results obtained indicate that the removal of mannose, galactose and fucose from cell membranes reduces the attachment of the virus to cell receptors.
Abstract: Chlamydia pneumoniae, a pathogenic bacteria responsible for respiratory tract infection... more Abstract: Chlamydia pneumoniae, a pathogenic bacteria responsible for respiratory tract infections, is known as the most implicated infectious agent in atherosclerotic cardiovascular diseases (CVDs). Accumulating evidence suggests that C. pneumoniae-induced oxidative stress may play a critical role in the pathogenesis of CVDs. Indeed, the overproduction of reactive oxygen species (ROS) within macrophages, endothelial cells, platelets and vascular smooth muscle cells (VSMCs) after C. pneumoniae exposure, has been shown to cause low density lipoprotein oxidation, foam cell formation, endothelial dysfunction, platelet adhesion and aggregation, and VSMC proliferation and migration, all responsible for the typical pathological changes of atherosclerotic plaque. The aim of this review is to improve our insight into C. pneumoniae-induced oxidative stress in order to suggest potential strategies for CVD prevention. Several antioxidants, acting on multi-enzymatic targets related to ROS produ...
The relationship between Con A-receptor mobility and Con A-induced agglutination of Novikoff hepa... more The relationship between Con A-receptor mobility and Con A-induced agglutination of Novikoff hepatoma and normal rat liver cells was investigated. Novikoff cells, incubated with fluorescein-labelled Con A at 3 °C displayed uniform, ring-like surface fluorescence. Increasing the temperature of the cells to 37 °C caused capping of Con A receptors in approximately 65% of the cells, a phenomenon that could be prevented by prefixing the cells with glutaraldehyde. In spite of these variations in Con A-receptor distribution, Con A-induced agglutination was remarkably constant over a temperature range from 3 to 37 °C. In contrast to Novikoff cells, normal hepatocytes displayed a uniform, ringlike surface fluorescence at both 3 and 37 °C. No capping was observed. However, hepatocytes, similar to Novikoff cells, were agglutinable by low concentrations of Con A. These findings indicate that, in this model system, Con A-induced cytoagglutination is not dependent upon long-range lateral mobility...
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Papers by Paola Mastromarino