Fibromiyalji sendromu (FMS), diğer semptomlarla ilişkili kronik kas-iskelet sistemi ağrı bozukluğ... more Fibromiyalji sendromu (FMS), diğer semptomlarla ilişkili kronik kas-iskelet sistemi ağrı bozukluğudur. Esas olarak ağrı, yorgunluk ve uyku bozukluğu ile karakterizedir. Fibromiyaljinin etiyolojisinde genetik, immünolojik ve hormonal birçok faktör önemli rol oynamaktadır. Bu patolojik durum popülasyonun yaklaşık %3-10'unu etkilemektedir. Görülme sıklığı açısından, kadınlarda erkeklerden daha fazla görülmektedir. Kadınlar erkeklerden daha şiddetli belirtiler ve daha düşük ağrı eşiği göstermektedir. Bu yüzden araştırmaların çoğu kadın deneklere odaklanmıştır. MikroRNA'lar (miRNA), insan genlerinin en az %30' unu düzenlemektedirler. Son yıllarda miRNA’lar hastalık süreçlerinde ve fizyolojik yolaklarda gen ekspresyonunun önemli modülatörleri olarak tanımlanmıştır. Bununla birlikte her miRNA yüzlerce genin regülasyonundan sorumlu olabilir. miRNA’lar translasyonun engellenmesi veya hedef haberci RNA'nın yıkımı yoluyla transkripsiyon sonrası gen ekspresyonunu inhibe ederler....
AIM End-stage renal disease is a disease in which the kidney is not able to perform its functions... more AIM End-stage renal disease is a disease in which the kidney is not able to perform its functions. Kidney transplantation is the most effective treatment and cost-effective modality of renal replacement therapy for patients with end-stage renal disease. However, the most important problem in end-stage renal disease patients is the unpredictability of immunologic response after transplants. In this study, it was aimed to investigate the possible association between the interleukin 2 (IL-2) expression level and an organ rejection or rejection episode. MATERIALS AND METHODS Lymphocytes were isolated from peripheral blood obtained from 21 end-stage renal disease-diagnosed patients prior to transplant and at the sixth month after transplant. CD4+ T cells were separated from lymphocytes by the magnetic cell-sorting method. The purity of these cells were controlled by a flow cytometer. After total RNA isolation from CD4+ T cells, IL-2 was examined by the real-time polymerase chain reaction (RT-PCR) method. RESULTS Among nonrejection patients (n = 18), the IL-2 expression level decreased in 12 patients in post-transplant time, and 3 of these were statistically significant (P < .05). The level was the same in 1 of 18 patients; it increased in 5 patients, and 1 of them was significant (P < .05). The IL-2 expression level also increased in 3 patients who had a rejection episode, and the increase was statistically significant in 2 samples (P < .05). CONCLUSION When the patients were evaluated individually, it was observed that there might be a relationship between IL-2 expression levels in CD4+ T cells and rejection episodes. The clinical data of the patients, the immunosuppressive therapies, and post-transplant evaluation of cytokines should be considered together.
Aim: Tyrosine kinase inhibitors are a targeted and successful treatment modalities in the treatme... more Aim: Tyrosine kinase inhibitors are a targeted and successful treatment modalities in the treatment of Chronic Myeloid Leukemia (CML). Imatinib mesylate is the most common of these inhibitors, but resistance reactions limit its use. It is believed that regulators in the Wnt signaling pathway play an important role in the treatment of CML. The aim of this study is to investigate possible expression changes of intracellular negative regulator genes after imatinib mesylate treatment in chronic myeloid leukemia cell line. Materials and Methods: Total RNA isolation was performed from K562 cells treated with Imatinib mesylate for 24 hours at 0.5 μM concentration and control native K562 cells. Following cDNA synthesis, expression changes of eight intracellular negative regulator genes were analyzed by Real-Time PCR. Results: When the results were evaluated, it was determined that the expression of 5 genes increased and the expression of 3 genes decreased in K562 cells treated with imatinib...
Chronic myeloid leukemia is a clonal hematopoietic stem cell disease characterized by myeloid exp... more Chronic myeloid leukemia is a clonal hematopoietic stem cell disease characterized by myeloid expansion. The hallmark of the disease is the Philadelphia chromosome, which results in the formation of the BCR-ABL oncogene, a tyrosine kinase that is involved in many signaling pathways including Wnt signaling. The latter has a unique role in chronic myeloid leukemia progression; activated signaling leads to the establishment of an additional leukemic stem cell population derived from granulocyte-macrophage progenitors. sFRP1 is an inhibitor of Wnt signaling and its expression is important for differentiation and maintenance of hematopoietic stem cells. In this study, we aimed to investigate miRNAs that target Wnt signaling by being co-induced along with the expression of sFRP1 in K562 cells. We present a detailed analysis of hsa-mir-221-3p, hsa-mir-222-3p, hsa-mir-106b-5p, hsa-let-7f-5p, hsa-mir-182-5p, hsa-mir-191-5p, and hsa-mir-183-5p and their target genes and their involvement in Wnt signaling.
Objectives There are several hypotheses on the effects of the rs1738074 T/C single nucleotide pol... more Objectives There are several hypotheses on the effects of the rs1738074 T/C single nucleotide polymorphism in the TAGAP gene; however, there has been no study on Turkish pediatric patients. We aimed to investigate the association of celiac disease (CD) and type 1 diabetes mellitus (T1DM) comorbidity with the polymorphism in the TAGAP gene of Turkish pediatric patients. Methods Totally, 127 pediatric CD patients and 100 healthy children were included. We determined the polymorphism by the allele-specific polymerase chain reaction method. We used IBM SPSS Statistics version 25.0 and Arlequin 3.5.2 for the statistical analyses. The authors have no conflict of interest. Results It was determined that 72% (n=154) of only CD patients had C allele, whereas 28% (n=60) had T allele. Of the patients with celiac and T1DM, 42.5% (n=17) and 57.5% (n=23) had T and C alleles, respectively. Of the individuals in control group, 67% (n=134) had C allele, whereas 33% (n=66) had T allele. Conclusions T...
BACKGROUND Wnt signaling cascades play important roles in cell fate decisions and their deregulat... more BACKGROUND Wnt signaling cascades play important roles in cell fate decisions and their deregulation has been documented in many diseases, including malignant tumors and leukemia. One mechanism of aberrant Wnt signaling is the silencing of Wnt inhibitors through epigenetic mechanisms. The sFRPs are one of the most studied Wnt inhibitors; and the sFRP1 loss is known in many hematological malignancies. Therefore, we aimed to compare the expression of Wnt related genes in the presence and absence of sFRP1 in chronic myeloid leukemia (CML) cell line. OBJECTIVE It is important to understand how sFRP1 and sFRP1 perform on CML to design new agents and strategies for resistant and advanced forms of CML. MATERIALS AND METHODS We used K562 cells, which normally do not express sFRP1 and its sFRP1 expressing subclone K562s. Total RNA was isolated from K562 and K562s cell lines end converted cDNA. PCR Array experiments performed using Human Wnt Signaling Pathway Plus RT2 Profiler™ kit. Wnt signa...
Amac: Kronik Miyeloid Losemi (KML), hematopoetik kok hucre (HKH) orijinli klonal miyeloproliferat... more Amac: Kronik Miyeloid Losemi (KML), hematopoetik kok hucre (HKH) orijinli klonal miyeloproliferatif bir hastaliktir. KML’deki Bcr-Abl kimerik geni ile birlikte diger ek mutasyonlar ve epigenetik modifikasyonlar hastaligin blastik faza dogru ilerleyisi icin gereklidir. DNA metiltransferazlar (DNMTler), epigenetik olarak duzenlenmis genlerin ifade edilmesinde ve baskilanmasinda onemli rol oynayan genom metilasyonu icin anahtar proteinlerdir. Hematolojik malignitelerde DNMT3A ve diger DNA metilasyon duzenleyicilerinde mutasyonlar tanimlanmistir. R882H mutasyonu DNMT3a’da en sik gozlenen mutasyondur. Bu calismanin amaci DNMT3A R882H mutasyonunun KML hastalarindaki gorulme oranini arastirmaktir. Gerec ve Yontem: Bcr/Abl kimerik gen analizi amaciyla gonderilmis KML hastalarinin kemik iliklerinden elde edilmis olan cDNA orneklerinde, DNMT3A R882H mutasyonunu taramak icin, AciI restriksiyon enzim kesimi kullanildi. Kesim sonrasi Bcr/Abl+ ve Bcr/Abl- orneklerden random secilerek DNMT3A icin ...
Purpose: In the present study, Glycodelin A mRNA expression levels of ectopic and etopic endometr... more Purpose: In the present study, Glycodelin A mRNA expression levels of ectopic and etopic endometrial tissues were examined in the secretion and proliferation stages of women with endometriosis compared to healthy individuals. Materials and Methods: 19 Patients with endometriosis, which were diagnosed during laparoscopy and laparotomy and confirmed histopathologically, and 7 control groups were included in the study of people between the age of 26-45. It was converted to cDNA after RNA isolation was performed from ectopic and etopic endometrial tissues taken during the secretion and proliferation stages of women with endometriosis and control group endometrial tissues. Glycodelin A expression levels were evaluated using real-time polymerase chain reaction. Results: Glycodelin A expression levels were higher in the euphoric and ectopic tissues of the patients with endometriosis in the secretory and proliferating phases. The Glycodelin A level of patients in the ectopic secretory phase...
Genomic studies have shown that approximately 2% of the human genome encodes protein, and the res... more Genomic studies have shown that approximately 2% of the human genome encodes protein, and the rest consists of non-coding RNAs (ncRNA). ncRNAs are the modifications that can alter gene expression at different levels without making any changes on the DNA sequence. One of the most important function of non-coding RNAs is to modulate host immunity and inflammatory response by regulating chromatin structure and gene expression. The World Health Organization has declared Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) as Coronavirus Disease 2019 (COVID-19). COVID-19 shows symptoms such as fever, cough, shortness of breath and it can lead to severe symptomatic acute respiratory distress syndrome (ARDS), bring along many problems such as cardiovascular complications, kidney damage, stroke and death. Virus host interactions associated with the immune system is also important beside the viral replication mechanism in the development of a successful therapeutic target for SARS-Co...
Fibromiyalji sendromu (FMS), diğer semptomlarla ilişkili kronik kas-iskelet sistemi ağrı bozukluğ... more Fibromiyalji sendromu (FMS), diğer semptomlarla ilişkili kronik kas-iskelet sistemi ağrı bozukluğudur. Esas olarak ağrı, yorgunluk ve uyku bozukluğu ile karakterizedir. Fibromiyaljinin etiyolojisinde genetik, immünolojik ve hormonal birçok faktör önemli rol oynamaktadır. Bu patolojik durum popülasyonun yaklaşık %3-10'unu etkilemektedir. Görülme sıklığı açısından, kadınlarda erkeklerden daha fazla görülmektedir. Kadınlar erkeklerden daha şiddetli belirtiler ve daha düşük ağrı eşiği göstermektedir. Bu yüzden araştırmaların çoğu kadın deneklere odaklanmıştır. MikroRNA'lar (miRNA), insan genlerinin en az %30' unu düzenlemektedirler. Son yıllarda miRNA’lar hastalık süreçlerinde ve fizyolojik yolaklarda gen ekspresyonunun önemli modülatörleri olarak tanımlanmıştır. Bununla birlikte her miRNA yüzlerce genin regülasyonundan sorumlu olabilir. miRNA’lar translasyonun engellenmesi veya hedef haberci RNA'nın yıkımı yoluyla transkripsiyon sonrası gen ekspresyonunu inhibe ederler....
AIM End-stage renal disease is a disease in which the kidney is not able to perform its functions... more AIM End-stage renal disease is a disease in which the kidney is not able to perform its functions. Kidney transplantation is the most effective treatment and cost-effective modality of renal replacement therapy for patients with end-stage renal disease. However, the most important problem in end-stage renal disease patients is the unpredictability of immunologic response after transplants. In this study, it was aimed to investigate the possible association between the interleukin 2 (IL-2) expression level and an organ rejection or rejection episode. MATERIALS AND METHODS Lymphocytes were isolated from peripheral blood obtained from 21 end-stage renal disease-diagnosed patients prior to transplant and at the sixth month after transplant. CD4+ T cells were separated from lymphocytes by the magnetic cell-sorting method. The purity of these cells were controlled by a flow cytometer. After total RNA isolation from CD4+ T cells, IL-2 was examined by the real-time polymerase chain reaction (RT-PCR) method. RESULTS Among nonrejection patients (n = 18), the IL-2 expression level decreased in 12 patients in post-transplant time, and 3 of these were statistically significant (P < .05). The level was the same in 1 of 18 patients; it increased in 5 patients, and 1 of them was significant (P < .05). The IL-2 expression level also increased in 3 patients who had a rejection episode, and the increase was statistically significant in 2 samples (P < .05). CONCLUSION When the patients were evaluated individually, it was observed that there might be a relationship between IL-2 expression levels in CD4+ T cells and rejection episodes. The clinical data of the patients, the immunosuppressive therapies, and post-transplant evaluation of cytokines should be considered together.
Aim: Tyrosine kinase inhibitors are a targeted and successful treatment modalities in the treatme... more Aim: Tyrosine kinase inhibitors are a targeted and successful treatment modalities in the treatment of Chronic Myeloid Leukemia (CML). Imatinib mesylate is the most common of these inhibitors, but resistance reactions limit its use. It is believed that regulators in the Wnt signaling pathway play an important role in the treatment of CML. The aim of this study is to investigate possible expression changes of intracellular negative regulator genes after imatinib mesylate treatment in chronic myeloid leukemia cell line. Materials and Methods: Total RNA isolation was performed from K562 cells treated with Imatinib mesylate for 24 hours at 0.5 μM concentration and control native K562 cells. Following cDNA synthesis, expression changes of eight intracellular negative regulator genes were analyzed by Real-Time PCR. Results: When the results were evaluated, it was determined that the expression of 5 genes increased and the expression of 3 genes decreased in K562 cells treated with imatinib...
Chronic myeloid leukemia is a clonal hematopoietic stem cell disease characterized by myeloid exp... more Chronic myeloid leukemia is a clonal hematopoietic stem cell disease characterized by myeloid expansion. The hallmark of the disease is the Philadelphia chromosome, which results in the formation of the BCR-ABL oncogene, a tyrosine kinase that is involved in many signaling pathways including Wnt signaling. The latter has a unique role in chronic myeloid leukemia progression; activated signaling leads to the establishment of an additional leukemic stem cell population derived from granulocyte-macrophage progenitors. sFRP1 is an inhibitor of Wnt signaling and its expression is important for differentiation and maintenance of hematopoietic stem cells. In this study, we aimed to investigate miRNAs that target Wnt signaling by being co-induced along with the expression of sFRP1 in K562 cells. We present a detailed analysis of hsa-mir-221-3p, hsa-mir-222-3p, hsa-mir-106b-5p, hsa-let-7f-5p, hsa-mir-182-5p, hsa-mir-191-5p, and hsa-mir-183-5p and their target genes and their involvement in Wnt signaling.
Objectives There are several hypotheses on the effects of the rs1738074 T/C single nucleotide pol... more Objectives There are several hypotheses on the effects of the rs1738074 T/C single nucleotide polymorphism in the TAGAP gene; however, there has been no study on Turkish pediatric patients. We aimed to investigate the association of celiac disease (CD) and type 1 diabetes mellitus (T1DM) comorbidity with the polymorphism in the TAGAP gene of Turkish pediatric patients. Methods Totally, 127 pediatric CD patients and 100 healthy children were included. We determined the polymorphism by the allele-specific polymerase chain reaction method. We used IBM SPSS Statistics version 25.0 and Arlequin 3.5.2 for the statistical analyses. The authors have no conflict of interest. Results It was determined that 72% (n=154) of only CD patients had C allele, whereas 28% (n=60) had T allele. Of the patients with celiac and T1DM, 42.5% (n=17) and 57.5% (n=23) had T and C alleles, respectively. Of the individuals in control group, 67% (n=134) had C allele, whereas 33% (n=66) had T allele. Conclusions T...
BACKGROUND Wnt signaling cascades play important roles in cell fate decisions and their deregulat... more BACKGROUND Wnt signaling cascades play important roles in cell fate decisions and their deregulation has been documented in many diseases, including malignant tumors and leukemia. One mechanism of aberrant Wnt signaling is the silencing of Wnt inhibitors through epigenetic mechanisms. The sFRPs are one of the most studied Wnt inhibitors; and the sFRP1 loss is known in many hematological malignancies. Therefore, we aimed to compare the expression of Wnt related genes in the presence and absence of sFRP1 in chronic myeloid leukemia (CML) cell line. OBJECTIVE It is important to understand how sFRP1 and sFRP1 perform on CML to design new agents and strategies for resistant and advanced forms of CML. MATERIALS AND METHODS We used K562 cells, which normally do not express sFRP1 and its sFRP1 expressing subclone K562s. Total RNA was isolated from K562 and K562s cell lines end converted cDNA. PCR Array experiments performed using Human Wnt Signaling Pathway Plus RT2 Profiler™ kit. Wnt signa...
Amac: Kronik Miyeloid Losemi (KML), hematopoetik kok hucre (HKH) orijinli klonal miyeloproliferat... more Amac: Kronik Miyeloid Losemi (KML), hematopoetik kok hucre (HKH) orijinli klonal miyeloproliferatif bir hastaliktir. KML’deki Bcr-Abl kimerik geni ile birlikte diger ek mutasyonlar ve epigenetik modifikasyonlar hastaligin blastik faza dogru ilerleyisi icin gereklidir. DNA metiltransferazlar (DNMTler), epigenetik olarak duzenlenmis genlerin ifade edilmesinde ve baskilanmasinda onemli rol oynayan genom metilasyonu icin anahtar proteinlerdir. Hematolojik malignitelerde DNMT3A ve diger DNA metilasyon duzenleyicilerinde mutasyonlar tanimlanmistir. R882H mutasyonu DNMT3a’da en sik gozlenen mutasyondur. Bu calismanin amaci DNMT3A R882H mutasyonunun KML hastalarindaki gorulme oranini arastirmaktir. Gerec ve Yontem: Bcr/Abl kimerik gen analizi amaciyla gonderilmis KML hastalarinin kemik iliklerinden elde edilmis olan cDNA orneklerinde, DNMT3A R882H mutasyonunu taramak icin, AciI restriksiyon enzim kesimi kullanildi. Kesim sonrasi Bcr/Abl+ ve Bcr/Abl- orneklerden random secilerek DNMT3A icin ...
Purpose: In the present study, Glycodelin A mRNA expression levels of ectopic and etopic endometr... more Purpose: In the present study, Glycodelin A mRNA expression levels of ectopic and etopic endometrial tissues were examined in the secretion and proliferation stages of women with endometriosis compared to healthy individuals. Materials and Methods: 19 Patients with endometriosis, which were diagnosed during laparoscopy and laparotomy and confirmed histopathologically, and 7 control groups were included in the study of people between the age of 26-45. It was converted to cDNA after RNA isolation was performed from ectopic and etopic endometrial tissues taken during the secretion and proliferation stages of women with endometriosis and control group endometrial tissues. Glycodelin A expression levels were evaluated using real-time polymerase chain reaction. Results: Glycodelin A expression levels were higher in the euphoric and ectopic tissues of the patients with endometriosis in the secretory and proliferating phases. The Glycodelin A level of patients in the ectopic secretory phase...
Genomic studies have shown that approximately 2% of the human genome encodes protein, and the res... more Genomic studies have shown that approximately 2% of the human genome encodes protein, and the rest consists of non-coding RNAs (ncRNA). ncRNAs are the modifications that can alter gene expression at different levels without making any changes on the DNA sequence. One of the most important function of non-coding RNAs is to modulate host immunity and inflammatory response by regulating chromatin structure and gene expression. The World Health Organization has declared Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) as Coronavirus Disease 2019 (COVID-19). COVID-19 shows symptoms such as fever, cough, shortness of breath and it can lead to severe symptomatic acute respiratory distress syndrome (ARDS), bring along many problems such as cardiovascular complications, kidney damage, stroke and death. Virus host interactions associated with the immune system is also important beside the viral replication mechanism in the development of a successful therapeutic target for SARS-Co...
Uploads
Papers by Melek Pehlivan