Introduction: Diabetes is a group of metabolic disorders characterized by high blood sugar levels... more Introduction: Diabetes is a group of metabolic disorders characterized by high blood sugar levels. The etiology is represented by a complex interaction between genetic and environmental factors. Metabolic disorders contribute to the pathogenesis of insulin resistance and of diabetes mellitus development. The insulin`s action in adipocytes is associated with an increase of lipolysis and concentration of free fatty acids. Stress plays a significant role in diabetes`s etiology and affects the disease`s evolution (debut, progression, and chronicity) through behavior changes. An analysis was performed based on smoker status and includes other information about patients' comorbidities. The study is descriptive and retrospective, analyzing 47 patients based on inclusion criteria (patients with type 2 diabetes, people with determinations of lipid profile and blood glucose, females and males, patients with/without BMI values, patients with/without other existing pathologies, patients wit...
Introduction: Diagnosis of diabetes mainly involves laboratory tests, which implies a high accura... more Introduction: Diagnosis of diabetes mainly involves laboratory tests, which implies a high accuracy in accordance with good laboratory practices. There is a wide range of tests that shows valuable information about the patient’s glycemic curve, nutrition, insulin resistance, metabolic products, but also about the lipid status of patients. Objective: The present paper aims to highlight the importance of laboratory tests in non-insulin dependent (type II) Diabetes mellitus, but also to determine the correlations between these parameters (glycated hemoglobin, blood sugar, triglycerides, total cholesterol, LDL and HDL cholesterol, lipids) byexploring a clinical case. Discussions: For most correlations, were obtained positive values, grater than 0.5 (direct correlations the value of one parameter decreases in time and manner with another). Following the analysis of thelaboratory parameters, resulted the direct relationship between the persistence of high values of glycated hemoglobin, bl...
Pumpkin (Cucurbita maxima) seeds are a rich source of ingredients such as aminoacids, fatty acids... more Pumpkin (Cucurbita maxima) seeds are a rich source of ingredients such as aminoacids, fatty acids, minerals, and phytochemicals exhibiting nutraceutical effects on human health. In this work, partially defatted pumpkin seeds flour, a by-product obtained during the manufacture of pumpkin seeds oil, was studied as an additive for common wheat flour. We explored the physico-chemical properties as well the content in amino acids of the partially defatted pumpkin seeds. The obtained results revealed that partially defatted pumpkin seeds are a good source of protein (42.75% d.m.), lipids (12.28% d.m.), total carbohydrates (37.4% d.m.), from which crude fiber (26.64% d.m.). This by-product presents a high mineral content (mg/100g): potassium (1290), magnesium (693), iron (87.8), zinc (11.5) and copper (2.49).The partially defatted pumpkin seeds proteins contain significant amounts of essential amino acids such as valine, histidine, isoleucine, leucine, threonine and methionine.
Grape skin is considered a valuable by-product for antioxidant and antibacterial agent preparatio... more Grape skin is considered a valuable by-product for antioxidant and antibacterial agent preparation. The grape skin contains some active compounds, such as, dietary fiber, polyphenols, flavonols, and resveratrol; it is commonly used as a nutritional supplement. The main aim of this study was to establish the optimum dose of grape skin flour, to be used as a functional ingredient in the bakery products industry, from both chemical and rheological point of view. The laboratory experiments evaluated the functional potential of wheat flour enriched with grape skin flour, in different proportions, by examining the chemical composition and rheological behaviour of the doughs. Protein, crude fibre, fat, ash and mineral contents were determined. Using �H-NMR spectral technique, the fatty acids composition was determined, especially the concentrations of short-chain saturated fatty acids (C4-C8), di-unsaturated fatty acids, mono-unsaturated fatty acids and long-chain saturated fatty acids (]C...
The object of this chapter is a short review of appropriate methods for the identification and au... more The object of this chapter is a short review of appropriate methods for the identification and authentication of plant materials and the quantification of bioactive compounds having physiological (or toxicological) relevance. The quality control of a botanical can be applied to raw material, a derivative (extract) or an ingredient of a commercial product (Plant Food Supplement or Traditional Medicine). Using the most suitable assays, each batch of botanical should be identified using taxonomic classification, morphological examination and/or biochemical/chemical characterization. An example of the quality control, which can be performed on botanicals, is here presented taking into consideration Camellia sinensis (tea)
The European PlantLIBRA research project, aiming to foster the safe use of food supplements conta... more The European PlantLIBRA research project, aiming to foster the safe use of food supplements containing plants or botanical preparation, along its four years of activity has achieved the following main objectives: - the creation of a metadata base containing data on: consumption, risks and benefits, biological activity of constituents, contaminants, methods to identify and control PFS; - the organization of a Network of laboratories with expertise in the analysis of plant/extract/plant food supplements. For the ultimate the protection of the consumers the quality and safety of herbal ingredients must be guaranteed emphasizing the need for stricter regulations and controls of botanical products by specialized laboratories. For this purpose PlantLIBRA has created an international network of laboratories with the aim of providing an integrated resource made available for decision-makers, both in the public and private sectors. The main goals of the network are: \u2022 provide reliable scientific support regarding methods for the identification of plant material, analysis of bioactive constituents that have positive or negative health effects, detection of environmental contaminants or residues from agriculture activities, analysis of biological markers useful to evaluate exposure, \u2022 support the work of international organizations involved in the food safety together with food manufacturers in achieving high quality products; \u2022 provide analytical tools to European Poison Centers for rapid identification of plant ingredients involved in the adverse effects. In the poster the results related to the comprehensive information that demonstrated the competencies for each laboratory in different analytical fields, the tools and the way in which the network was organized are described
INHIBITORY ACTIVITY OF PYRETHROID PESTICIDES ON GLUTATHIONE S-TRANSFERASE. Glutathione S-transfer... more INHIBITORY ACTIVITY OF PYRETHROID PESTICIDES ON GLUTATHIONE S-TRANSFERASE. Glutathione S-transferase (GST) is one of the enzymes used to evaluate the effects caused by environmental contaminants. The aim of this work was to study the effects of four pyrethroid compounds (allethrin, cypermethrin, deltamethrin and fenpropathrin) on the catalytic activity of GST and to estimate the potential for environmental contamination in silico of these insecticides. Spectrophotometric analyses showed that the activity of the GST enzyme was inhibited by the four pesticides, with the values for the inhibition constant (Ki) varying from 0.12 to 20 µmol L-1, with the following order of inhibition from the highest to the lowest Ki value: deltamethrin > cypermethrin > allethrin > fenpropathrin. They presented a competitive and non-competitive inhibition mechanism depending on the compound. The IC50 values (concentration that inhibits 50% of the enzyme activity) showed the insecticide deltameth...
Introduction: Diabetes is a group of metabolic disorders characterized by high blood sugar levels... more Introduction: Diabetes is a group of metabolic disorders characterized by high blood sugar levels. The etiology is represented by a complex interaction between genetic and environmental factors. Metabolic disorders contribute to the pathogenesis of insulin resistance and of diabetes mellitus development. The insulin`s action in adipocytes is associated with an increase of lipolysis and concentration of free fatty acids. Stress plays a significant role in diabetes`s etiology and affects the disease`s evolution (debut, progression, and chronicity) through behavior changes. An analysis was performed based on smoker status and includes other information about patients' comorbidities. The study is descriptive and retrospective, analyzing 47 patients based on inclusion criteria (patients with type 2 diabetes, people with determinations of lipid profile and blood glucose, females and males, patients with/without BMI values, patients with/without other existing pathologies, patients wit...
Introduction: Diagnosis of diabetes mainly involves laboratory tests, which implies a high accura... more Introduction: Diagnosis of diabetes mainly involves laboratory tests, which implies a high accuracy in accordance with good laboratory practices. There is a wide range of tests that shows valuable information about the patient’s glycemic curve, nutrition, insulin resistance, metabolic products, but also about the lipid status of patients. Objective: The present paper aims to highlight the importance of laboratory tests in non-insulin dependent (type II) Diabetes mellitus, but also to determine the correlations between these parameters (glycated hemoglobin, blood sugar, triglycerides, total cholesterol, LDL and HDL cholesterol, lipids) byexploring a clinical case. Discussions: For most correlations, were obtained positive values, grater than 0.5 (direct correlations the value of one parameter decreases in time and manner with another). Following the analysis of thelaboratory parameters, resulted the direct relationship between the persistence of high values of glycated hemoglobin, bl...
Pumpkin (Cucurbita maxima) seeds are a rich source of ingredients such as aminoacids, fatty acids... more Pumpkin (Cucurbita maxima) seeds are a rich source of ingredients such as aminoacids, fatty acids, minerals, and phytochemicals exhibiting nutraceutical effects on human health. In this work, partially defatted pumpkin seeds flour, a by-product obtained during the manufacture of pumpkin seeds oil, was studied as an additive for common wheat flour. We explored the physico-chemical properties as well the content in amino acids of the partially defatted pumpkin seeds. The obtained results revealed that partially defatted pumpkin seeds are a good source of protein (42.75% d.m.), lipids (12.28% d.m.), total carbohydrates (37.4% d.m.), from which crude fiber (26.64% d.m.). This by-product presents a high mineral content (mg/100g): potassium (1290), magnesium (693), iron (87.8), zinc (11.5) and copper (2.49).The partially defatted pumpkin seeds proteins contain significant amounts of essential amino acids such as valine, histidine, isoleucine, leucine, threonine and methionine.
Grape skin is considered a valuable by-product for antioxidant and antibacterial agent preparatio... more Grape skin is considered a valuable by-product for antioxidant and antibacterial agent preparation. The grape skin contains some active compounds, such as, dietary fiber, polyphenols, flavonols, and resveratrol; it is commonly used as a nutritional supplement. The main aim of this study was to establish the optimum dose of grape skin flour, to be used as a functional ingredient in the bakery products industry, from both chemical and rheological point of view. The laboratory experiments evaluated the functional potential of wheat flour enriched with grape skin flour, in different proportions, by examining the chemical composition and rheological behaviour of the doughs. Protein, crude fibre, fat, ash and mineral contents were determined. Using �H-NMR spectral technique, the fatty acids composition was determined, especially the concentrations of short-chain saturated fatty acids (C4-C8), di-unsaturated fatty acids, mono-unsaturated fatty acids and long-chain saturated fatty acids (]C...
The object of this chapter is a short review of appropriate methods for the identification and au... more The object of this chapter is a short review of appropriate methods for the identification and authentication of plant materials and the quantification of bioactive compounds having physiological (or toxicological) relevance. The quality control of a botanical can be applied to raw material, a derivative (extract) or an ingredient of a commercial product (Plant Food Supplement or Traditional Medicine). Using the most suitable assays, each batch of botanical should be identified using taxonomic classification, morphological examination and/or biochemical/chemical characterization. An example of the quality control, which can be performed on botanicals, is here presented taking into consideration Camellia sinensis (tea)
The European PlantLIBRA research project, aiming to foster the safe use of food supplements conta... more The European PlantLIBRA research project, aiming to foster the safe use of food supplements containing plants or botanical preparation, along its four years of activity has achieved the following main objectives: - the creation of a metadata base containing data on: consumption, risks and benefits, biological activity of constituents, contaminants, methods to identify and control PFS; - the organization of a Network of laboratories with expertise in the analysis of plant/extract/plant food supplements. For the ultimate the protection of the consumers the quality and safety of herbal ingredients must be guaranteed emphasizing the need for stricter regulations and controls of botanical products by specialized laboratories. For this purpose PlantLIBRA has created an international network of laboratories with the aim of providing an integrated resource made available for decision-makers, both in the public and private sectors. The main goals of the network are: \u2022 provide reliable scientific support regarding methods for the identification of plant material, analysis of bioactive constituents that have positive or negative health effects, detection of environmental contaminants or residues from agriculture activities, analysis of biological markers useful to evaluate exposure, \u2022 support the work of international organizations involved in the food safety together with food manufacturers in achieving high quality products; \u2022 provide analytical tools to European Poison Centers for rapid identification of plant ingredients involved in the adverse effects. In the poster the results related to the comprehensive information that demonstrated the competencies for each laboratory in different analytical fields, the tools and the way in which the network was organized are described
INHIBITORY ACTIVITY OF PYRETHROID PESTICIDES ON GLUTATHIONE S-TRANSFERASE. Glutathione S-transfer... more INHIBITORY ACTIVITY OF PYRETHROID PESTICIDES ON GLUTATHIONE S-TRANSFERASE. Glutathione S-transferase (GST) is one of the enzymes used to evaluate the effects caused by environmental contaminants. The aim of this work was to study the effects of four pyrethroid compounds (allethrin, cypermethrin, deltamethrin and fenpropathrin) on the catalytic activity of GST and to estimate the potential for environmental contamination in silico of these insecticides. Spectrophotometric analyses showed that the activity of the GST enzyme was inhibited by the four pesticides, with the values for the inhibition constant (Ki) varying from 0.12 to 20 µmol L-1, with the following order of inhibition from the highest to the lowest Ki value: deltamethrin > cypermethrin > allethrin > fenpropathrin. They presented a competitive and non-competitive inhibition mechanism depending on the compound. The IC50 values (concentration that inhibits 50% of the enzyme activity) showed the insecticide deltameth...
Uploads
Papers by Mihaela Badea