Different periodontopathogenic microbiota have been associated with periodontal diseases in sever... more Different periodontopathogenic microbiota have been associated with periodontal diseases in several populations. The present investigation determined the subgingival microbiota of untreated chronic periodontitis Brazilians using the checkerboard DNA-DNA hybridization technique. Twenty-five periodontitis patients (mean age, 41 +/- 2; mean probing depth [PD], 3.3 +/- 0.2; mean attachment level [AL], 3.6 +/- 0.2) with no history of previous periodontal therapy and a control group of 14 healthy subjects (mean age, 34 +/- 0.6; mean PD, 1.8 +/- 0.2; mean AL, 1.7 +/- 0.1) were selected. Measurements of PD, AL, bleeding on probing, plaque accumulation, and suppuration were recorded at 6 sites/tooth. Subgingival plaque samples were obtained from 4 sites in each tooth/subject in both groups. The presence and levels of 41 subgingival species were determined in 4,032 plaque samples using whole genomic DNA probes and the checkerboard method. Periodontal pathogens, as well as some unusual species (E. faecalis, E. coli and Bartonella sp.), were detected significantly more often and/or in higher levels in the periodontitis group (P < 0.05). Most species were more frequently detected in interproximal sites. B. forsythus, P. gingivalis, E. nodatum, and F. nucleatum ss vincentii showed a significant positive correlation with mean PD and AL (P < 0.05). The subgingival microbiota of Brazilians with untreated chronic periodontitis were complex, including high proportions of periodontopathogens commonly found in other populations, as well as some unusual species.
Infective endocarditis (IE) is still associated with high mortality, and antibiotic prophylaxis s... more Infective endocarditis (IE) is still associated with high mortality, and antibiotic prophylaxis strategies are under intense debate. We evaluated the incidence of bacteremia after root canal preparation in teeth with necrotic pulps and apical periodontitis. Blood samples were taken before and 5 and 30 minutes after endodontic treatment in teeth with apical periodontitis from individuals at high (n = 21) or no risk (n = 11) for IE. The former received prophylactic antibiotic therapy. Bacteriologic samples were taken from root canals before chemomechanical preparation to confirm pulp infection. Samples were subjected to aerobic and anaerobic culture and quantitative real-time polymerase chain reaction (qPCR), the latter to determine the total bacterial and streptococcal levels. Culture revealed no bacteremia in all individuals. Analysis by qPCR showed that bacterial DNA occurred in all root canal samples. qPCR showed a similar incidence of bacteremia between patients who received or d...
The antiseptic and/or soap use for hand washing is a currently action to any clinician of health ... more The antiseptic and/or soap use for hand washing is a currently action to any clinician of health areas. Despite, it is not consensual which substance would be better for this purpose. The aim of this study was to evaluate, comparatively, the immediate action of some products, which are: Hibitaneä (chlorhexidine 4%); Soapexä (irgasan DP-300); coco-nut soap Cariocaä (oil of coconut of babaçu, sodium hydroxide, sodium carbonate, calcium carbonate, sodium chloride and water); Antibacä (propylene glycol, EDTA, chloro xylenol 0,3%, triclosan); Povidineä (polyvinilpirolidine active iodine 10%) and 70% isopropyl alcohol. So, it was made a count of the number of colony forming units of hands of ten volunteers for the baseline and just after the use of these products. The result had been analyzed by Anova and Tukey Test. Only Hibitaneä, Povidineä and 70% isopropyl alcohol showed to be effective, among all the products in the immediate reduction of the hand microbiota.
... Renata Souto I, * ; Arnaldo Feitosa B. de Andrade II ; Milton Uzeda I ; Ana Paula Vieira Colo... more ... Renata Souto I, * ; Arnaldo Feitosa B. de Andrade II ; Milton Uzeda I ; Ana Paula Vieira Colombo I. ... Immunol., 7, 14-18, 1992. [ Links ]. 11. Haffajee, AD; Socransky, SS; Lindhe, J.; Kent, RL; Okamoto, H.; Yoneyama, T. Clinical risk indicators for periodontal attachment loss. J. Clin. ...
... Com base nos resultados obtidos e analisados estatisticamente pelo ANOVA e pelo teste de Tuke... more ... Com base nos resultados obtidos e analisados estatisticamente pelo ANOVA e pelo teste de Tukey, verificou-se que o dentifrício contendo xilitol, lactoperoxidase, glicose oxidase e lactoferrina eo com xilitol e extrato de Calendula officinalis näo apresentaram efeito ...
Resumo: Os autores fizeram um experimento com 65 meninas institucionalizadas divididas entre quat... more Resumo: Os autores fizeram um experimento com 65 meninas institucionalizadas divididas entre quatro grupos que durante 3 meses escovaram os dentes utilizando dentifrício contendo xilitol e fluoreto, outro contendo fluoreto, o terceiro um dentifrício sem xilitol e ...
This study investigates the association between detectable plasmatic human immunodeficiency virus... more This study investigates the association between detectable plasmatic human immunodeficiency virus (HIV) viral load (HVL) and high levels of periodontal- and non-periodontal-related microorganisms in the subgingival microbiota of individuals with HIV. Thirty-seven individuals with HIV were divided into two groups: 1) detectable HVL (n = 15); and 2) undetectable HVL (n = 22). Subgingival biofilm samples were obtained, and the levels of 35 microbial species were determined by the checkerboard DNA-DNA hybridization method. Periodontal clinical measures and laboratory and sociodemographic data were also registered. χ(2) test, Fisher exact test, and Mann-Whitney U test were used to compare groups. Multilevel ordinal regression models were used to test the association between HVL and the levels of 35 microbial species in subgingival biofilm, adjusted for confounders. Of the 35 species studied, 11 (31.4%) showed higher mean levels in the detectable HVL group than undetectable HVL group (P <0.001). These species included Actinomyces naeslundii II, Actinomyces israelii, Actinomyces odontolyticus, Veillonella parvula, Capnocytophaga gingivalis, Eikenella corrodens, Campylobacter concisus, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, and Candida albicans. Significant associations between detectable HVL and high levels of microorganisms, adjusted for confounders, were observed for A. naeslundii I, Actinomyces gerencseriae, C. gingivalis, E. corrodens, C. concisus, Prevotella nigrescens, T. forsythia, and Dialister pneumosintes. Detectable plasmatic HVL in individuals with HIV was associated with elevated levels of known periodontal pathogens, such as P. nigrescens, T. forsythia, and E. corrodens, as well as C. concisus, C. gingivalis, and D. pneumosintes in the subgingival biofilm.
Different periodontopathogenic microbiota have been associated with periodontal diseases in sever... more Different periodontopathogenic microbiota have been associated with periodontal diseases in several populations. The present investigation determined the subgingival microbiota of untreated chronic periodontitis Brazilians using the checkerboard DNA-DNA hybridization technique. Twenty-five periodontitis patients (mean age, 41 +/- 2; mean probing depth [PD], 3.3 +/- 0.2; mean attachment level [AL], 3.6 +/- 0.2) with no history of previous periodontal therapy and a control group of 14 healthy subjects (mean age, 34 +/- 0.6; mean PD, 1.8 +/- 0.2; mean AL, 1.7 +/- 0.1) were selected. Measurements of PD, AL, bleeding on probing, plaque accumulation, and suppuration were recorded at 6 sites/tooth. Subgingival plaque samples were obtained from 4 sites in each tooth/subject in both groups. The presence and levels of 41 subgingival species were determined in 4,032 plaque samples using whole genomic DNA probes and the checkerboard method. Periodontal pathogens, as well as some unusual species (E. faecalis, E. coli and Bartonella sp.), were detected significantly more often and/or in higher levels in the periodontitis group (P < 0.05). Most species were more frequently detected in interproximal sites. B. forsythus, P. gingivalis, E. nodatum, and F. nucleatum ss vincentii showed a significant positive correlation with mean PD and AL (P < 0.05). The subgingival microbiota of Brazilians with untreated chronic periodontitis were complex, including high proportions of periodontopathogens commonly found in other populations, as well as some unusual species.
Infective endocarditis (IE) is still associated with high mortality, and antibiotic prophylaxis s... more Infective endocarditis (IE) is still associated with high mortality, and antibiotic prophylaxis strategies are under intense debate. We evaluated the incidence of bacteremia after root canal preparation in teeth with necrotic pulps and apical periodontitis. Blood samples were taken before and 5 and 30 minutes after endodontic treatment in teeth with apical periodontitis from individuals at high (n = 21) or no risk (n = 11) for IE. The former received prophylactic antibiotic therapy. Bacteriologic samples were taken from root canals before chemomechanical preparation to confirm pulp infection. Samples were subjected to aerobic and anaerobic culture and quantitative real-time polymerase chain reaction (qPCR), the latter to determine the total bacterial and streptococcal levels. Culture revealed no bacteremia in all individuals. Analysis by qPCR showed that bacterial DNA occurred in all root canal samples. qPCR showed a similar incidence of bacteremia between patients who received or d...
The antiseptic and/or soap use for hand washing is a currently action to any clinician of health ... more The antiseptic and/or soap use for hand washing is a currently action to any clinician of health areas. Despite, it is not consensual which substance would be better for this purpose. The aim of this study was to evaluate, comparatively, the immediate action of some products, which are: Hibitaneä (chlorhexidine 4%); Soapexä (irgasan DP-300); coco-nut soap Cariocaä (oil of coconut of babaçu, sodium hydroxide, sodium carbonate, calcium carbonate, sodium chloride and water); Antibacä (propylene glycol, EDTA, chloro xylenol 0,3%, triclosan); Povidineä (polyvinilpirolidine active iodine 10%) and 70% isopropyl alcohol. So, it was made a count of the number of colony forming units of hands of ten volunteers for the baseline and just after the use of these products. The result had been analyzed by Anova and Tukey Test. Only Hibitaneä, Povidineä and 70% isopropyl alcohol showed to be effective, among all the products in the immediate reduction of the hand microbiota.
... Renata Souto I, * ; Arnaldo Feitosa B. de Andrade II ; Milton Uzeda I ; Ana Paula Vieira Colo... more ... Renata Souto I, * ; Arnaldo Feitosa B. de Andrade II ; Milton Uzeda I ; Ana Paula Vieira Colombo I. ... Immunol., 7, 14-18, 1992. [ Links ]. 11. Haffajee, AD; Socransky, SS; Lindhe, J.; Kent, RL; Okamoto, H.; Yoneyama, T. Clinical risk indicators for periodontal attachment loss. J. Clin. ...
... Com base nos resultados obtidos e analisados estatisticamente pelo ANOVA e pelo teste de Tuke... more ... Com base nos resultados obtidos e analisados estatisticamente pelo ANOVA e pelo teste de Tukey, verificou-se que o dentifrício contendo xilitol, lactoperoxidase, glicose oxidase e lactoferrina eo com xilitol e extrato de Calendula officinalis näo apresentaram efeito ...
Resumo: Os autores fizeram um experimento com 65 meninas institucionalizadas divididas entre quat... more Resumo: Os autores fizeram um experimento com 65 meninas institucionalizadas divididas entre quatro grupos que durante 3 meses escovaram os dentes utilizando dentifrício contendo xilitol e fluoreto, outro contendo fluoreto, o terceiro um dentifrício sem xilitol e ...
This study investigates the association between detectable plasmatic human immunodeficiency virus... more This study investigates the association between detectable plasmatic human immunodeficiency virus (HIV) viral load (HVL) and high levels of periodontal- and non-periodontal-related microorganisms in the subgingival microbiota of individuals with HIV. Thirty-seven individuals with HIV were divided into two groups: 1) detectable HVL (n = 15); and 2) undetectable HVL (n = 22). Subgingival biofilm samples were obtained, and the levels of 35 microbial species were determined by the checkerboard DNA-DNA hybridization method. Periodontal clinical measures and laboratory and sociodemographic data were also registered. χ(2) test, Fisher exact test, and Mann-Whitney U test were used to compare groups. Multilevel ordinal regression models were used to test the association between HVL and the levels of 35 microbial species in subgingival biofilm, adjusted for confounders. Of the 35 species studied, 11 (31.4%) showed higher mean levels in the detectable HVL group than undetectable HVL group (P <0.001). These species included Actinomyces naeslundii II, Actinomyces israelii, Actinomyces odontolyticus, Veillonella parvula, Capnocytophaga gingivalis, Eikenella corrodens, Campylobacter concisus, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, and Candida albicans. Significant associations between detectable HVL and high levels of microorganisms, adjusted for confounders, were observed for A. naeslundii I, Actinomyces gerencseriae, C. gingivalis, E. corrodens, C. concisus, Prevotella nigrescens, T. forsythia, and Dialister pneumosintes. Detectable plasmatic HVL in individuals with HIV was associated with elevated levels of known periodontal pathogens, such as P. nigrescens, T. forsythia, and E. corrodens, as well as C. concisus, C. gingivalis, and D. pneumosintes in the subgingival biofilm.
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