Objective(s): This paper reports on the toxicity of CuO NPs on hepatic enzymes and liver and lung... more Objective(s): This paper reports on the toxicity of CuO NPs on hepatic enzymes and liver and lung histology. Materials and Methods: To assess the toxicity of copper nanoparticles (10-15 nm) in vivo, pathological examinations and blood biochemical indexes including serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) at various time points (2, 7 and 14 days) were studied. Thirty two Wistar rats were randomly divided into four groups. Treatment groups (group 1, 2, 3) received CuO NP solution containing 5, 10 and 100 mg/kg, respectively. Control group received 0.5 mL of normal saline via ip injection for 7 consecutive days. After 14 days, the tissue of liver and lung were collected and investigated for their histological problems. Results: The histology of the hepatic tissues showed vasculature in central veins and portal triad vessels in all three treatment groups. Histology of lungs showed air sac wall thickening and increased fibrous tiss...
International Journal of Medical Research and Health Sciences, 2016
Food waste has been identified as a considerable problem and bread is the most wasted food. This ... more Food waste has been identified as a considerable problem and bread is the most wasted food. This study aimed to evaluate In-vitro anti-fungal activity of cinnamon extract on bread spoilage fungi and to determine its anti-fungal effect in the bread slices. At first, the MIC and MFC values of the extract were determined against Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus, Penicillium chrysogenum, Penicillium notatum and Rhizopus oryzae. Then, Aspergillus sp was selected to assess antifungal activities of different doses of cinnamon extract in bread slices. Cinnamon extract at a dose of 64 mg/ml completely inhibited all standard and bread isolated fungi. This concentration of extract also inhibited Aspergillus growth on bread slices and delayed colony formation but adversely affected the sensory characteristics of bread. Cinnamon extract at 32 mg/ml not only delayed fungal growth, but also improved bread shelf life and delayed its staling. Moreover, 32mg/ml of extract ...
Introduction: Diabetes is one of the most important metabolic diseases worldwide. Wound infection... more Introduction: Diabetes is one of the most important metabolic diseases worldwide. Wound infections due to antibiotic resistant bacteria can cause lower limbs ulceration and amputation in diabetic patients.The present study was performed with the aim of the evaluation of antibacterial effects of cellulose disc from kombuchaon bacteria isolated from diabetic foot ulcers. Materials and Methods: In this descriptive-analytical study,bacterial were isolated from diabetic wounds and identified based on biochemical and molecular characterization. Then the antibacterial effect of Kombucha cellulose layer wasevaluated on the isolates using disc diffusion (qualitative) and agar dilution (quantitative) methods, and the data was statisticaly analyzed. Results: The most frequency of pathogenic bacteria that isolated in the present study from diabetic wounds were included 56% Escherichia coli (E.coli), 22% Enterobacter cloacae (E. cloacae), 6% Citrobacter diversus (C. diversus), 4% for each of Ent...
Introduction Staphylococcus aureus and Staphylococcus epidermidis are widely recognized as ubiqui... more Introduction Staphylococcus aureus and Staphylococcus epidermidis are widely recognized as ubiquitous and pathogenic bacteria implicated in hospital-acquired infections on a global scale (1-3). S. aureus has been observed to colonize on the skin, particularly when damaged, as well as in various other areas including the perineum, vagina, armpit, navel of neonates, and oropharynx (4, 5). This gram-positive bacterium represents a significant causative agent in the domain of healthcare-associated infections (6). Methicillin-resistant S. aureus (MRSA) is a strain of S. aureus that is resistant to methicillin and other beta-lactam antibiotics. Methicillinresistant S. aureus (MRSA) constitutes a grave concern in the context of hospital-acquired infections, owing to its opportunistic nature, and exacerbates the complexity of treating infections caused by this bacterium (7,8). One of the contributory factors for the resistance of the bacteria to methicillin is the indiscriminate administration of antibiotics, particularly beta-lactam antibiotics. This may be attributed to the inadequate and uninformed
International Journal of Molecular and Clinical Microbiology, 2014
ARTICLE INFO ABSTRACT Article history: Received 3 February 2014 Accepted 27 March 2014 Available ... more ARTICLE INFO ABSTRACT Article history: Received 3 February 2014 Accepted 27 March 2014 Available online 1 June 2014 The use of herbal products in the prevention of the growth of pathogens have been widely studied. Problems in the treatment of infections caused by resistant strains of fungi are indicator of the need for a more in-detailed study of herbal remedies. Artemisia aucheri is used as a medicinal plant with anti-microbial properties, and as an astringent. Plant tissue culture is an important technique for the production of secondary metabolites. The aqueous and methanol extracts (AE and ME) were prepared from the resulting callus. In the agar well diffusion method in a concentration of 2500 mg/ml of AE, the growth inhibition zone diameter (IZD) of clinical isolates of C.albicans was greater than that of fluconazole and both standard strains of C.albicans (PTCC 1167 , 5027) was greater than the positive control. The results of the effect of AE showed that has the greatest effe...
Journal of Herbal Drugs: International Journal on Medicinal Herbs, 2016
Background & Aim: Vaginal disease has always been a great concern of women. Medicinal plants as n... more Background & Aim: Vaginal disease has always been a great concern of women. Medicinal plants as natural resources with fewer side effects can effectively treat these infections given the diversity of these infections, resistance to antibiotics and side effects of existing drugs. The present study aimed to compare antimicrobial effects of methanol extract of Francoeuria Undulata on growth of several gram-positive and gram-negative bacteria causing vaginal infections. Experimental: Francoeuria Undulata as an indigenous Iranian plant was collected in two harvest times (T1 and T2). Methanol extract of aerial parts of the plant was prepared. The effects of various concentrations (62.5, 125, 250 and 500 mg/ml)of methanol extracts were studied onKlebsiella and Enterobacter as gram-negative pathogens. Minimum Inhibitory Concentration (MIC) of bacterial growth and Minimum Bactericidal Concentration (MBC) were determined using macro-dilution method. Data analysis was performed using ANOVA. Re...
Journal of Research in Medical and Dental Science, 2018
In recent years, due to the indiscriminate use of synthetic drugs are irrational drug resistance ... more In recent years, due to the indiscriminate use of synthetic drugs are irrational drug resistance of pathogenic microorganisms is increasing the need for new compounds. The purpose of this study was to investigate the effect of ethanolic and aqueous extracts of Plantago psyllium seed on Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Citrobacter freundii, Enterobacter aerugenes, Pseudomonas aeruginosa and Acinetobacter baumannii, from hospitals (Shariati, Sadoughi, Garazi) and Vahid Medical Laboratory in zarrin shahr were isolated. Also, on the standard of bacteria. Plantago psyllium seed of Agriculture and Natural Resources Research Center of Isfahan was prepared. aqueous and ethanolic extracts were prepared by maceration method. After identification of bacteria, antibiotic resistance was determined by disk diffusion method. Antibacterial effect of ethanolic and aqueous extracts of P. psyllium seed on the growth of multi-drug resistant bacteria by plate diffusion in four...
International journal of Advanced Biological and Biomedical Research, 2013
The objective of this study was to isolation and characterization of Listeria species and determi... more The objective of this study was to isolation and characterization of Listeria species and determines Listeria monocytogenes serotypes in fresh fish, shrimp, crab and lobster in Isfahan and Shahrekord, Iran. From September 2010 to April 2011, a total of 300 samples of fresh fish, shrimp, crab and lobster were obtained from randomly selected retail stores in Isfahan and Shahrekord, Iran. The samples were tested for the presence of Listeria species using a ISO 11290 protocol and then only Listeria monocytogenes isolates identified by bacteriological methods were tested by polymerase chain reaction (PCR) for serotypes detection and presences of virulence associated genes. From the total 300 samples, 23 (10.45%) fresh fish and 1 (2.5%) shrimp samples were positive for Listeria spp., but none of the lobster and crab samples were positive for Listeria species. Listeria monocytogenes only isolated from 17 fish (7.25%) and 1 shrimp (2.5%) samples while L. innocua, L. ivanovii and L. seeliger...
Background: Using smoke from burning donkey dung has been popular in the treatment of many diseas... more Background: Using smoke from burning donkey dung has been popular in the treatment of many diseases in Iran. Objective: This study aimed to investigating the antimicrobial properties of donkey dung smoke on multi-drug resistant (MDR) bacteria isolated from urinary infection. Materials and Methods: First, 300 and 200 urine samples were collected from pregnant and non-pregnant women in Isfahan, Iran. Then in each group, 100 bacterial isolates including Escherichia coli, Klebsiella pneumonia, Proteus vulgaris, Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa, and Staphylococcus saprophyticus were isolated. Antibiotic resistant protocol was determined by antibiogram test. Donkey dung was sterilized, disintegrated, and heated. The smokes were concentrated in n-hexane solvent (65%) and were collected after evaporation of the solvent. Finally, the antibacterial activities of the concentrations of 0.25, 0.5 and 1 mg/mL of the smokes were detected using disk diffusio...
Journal of Gorgan University of Medical Sciences, 2013
Background and Objective: Pollution due to the heavy metals is a problem that may have negative c... more Background and Objective: Pollution due to the heavy metals is a problem that may have negative consequences on the hydrosphere. Identification of microorganisms resistant to heavy metals plays an important role in relation to environmental pollution bioremediation. This study was done to assess the antibiotic resistance in isolated bacteria from urban sewage and copper smeltery industrial wastewater. Materials and Methods: This laboratory study was done on the wastewater samples from urban sewage and copper smeltery in Isfahan-Iran, during 2011-12. Heavy metal resistant microorganisms were isolated and enumerated after serial dilution and culturing on PHG agar plates supplemented with 0.5 mM of each heavy metal. The pattern of resistance was assigned by Minimum Inhibitory Concentration (MIC). Antibiotic resistance toward following medicines Ofloxacine, Penicilline, Sulfometoxasole, Lincomycin, Kanamycin, Streptomycin, Clindamycin, Vancomycin, Cefradin and Neomycin were subsequently...
Antibacterial agents derived from plants are known as suitable alternatives for synthetic pestici... more Antibacterial agents derived from plants are known as suitable alternatives for synthetic pesticides because of their health and the lack of any side effects on plants. This study aims to assess in vitro effect of water, ethanol and methanol extracts of tobacco (Nicotiana plumbaginifolia) leaves on plant pathogenic bacteria and their effect on the control of bacterial infection in tomato plant in the farm conditions. Also, effective compounds of the selected extract were characterized. Water, ethanol and methanol extracts were prepared from the leaves of Nicotiana plumbaginifolia by soaking method. Antibacterial effects of three extracts were determined by agar well diffusion method in 100, 400 and 800 mg/ml concentrations on plant pathogenic bacteria including Xantomonas campestris PTCC1473, Pseudomonas aeruginosa PTCC1181, Pseudomonas syringae PTCC1290, Enterobacter aerogenes PTCC1221, Clavibacter michiganensis PTCC1399, Ralstonia solanacearum PTCC1600 and Gluconacetobacter PTCC17...
Objective(s): This paper reports on the toxicity of CuO NPs on hepatic enzymes and liver and lung... more Objective(s): This paper reports on the toxicity of CuO NPs on hepatic enzymes and liver and lung histology. Materials and Methods: To assess the toxicity of copper nanoparticles (10-15 nm) in vivo, pathological examinations and blood biochemical indexes including serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) at various time points (2, 7 and 14 days) were studied. Thirty two Wistar rats were randomly divided into four groups. Treatment groups (group 1, 2, 3) received CuO NP solution containing 5, 10 and 100 mg/kg, respectively. Control group received 0.5 mL of normal saline via ip injection for 7 consecutive days. After 14 days, the tissue of liver and lung were collected and investigated for their histological problems. Results: The histology of the hepatic tissues showed vasculature in central veins and portal triad vessels in all three treatment groups. Histology of lungs showed air sac wall thickening and increased fibrous tiss...
International Journal of Medical Research and Health Sciences, 2016
Food waste has been identified as a considerable problem and bread is the most wasted food. This ... more Food waste has been identified as a considerable problem and bread is the most wasted food. This study aimed to evaluate In-vitro anti-fungal activity of cinnamon extract on bread spoilage fungi and to determine its anti-fungal effect in the bread slices. At first, the MIC and MFC values of the extract were determined against Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus, Penicillium chrysogenum, Penicillium notatum and Rhizopus oryzae. Then, Aspergillus sp was selected to assess antifungal activities of different doses of cinnamon extract in bread slices. Cinnamon extract at a dose of 64 mg/ml completely inhibited all standard and bread isolated fungi. This concentration of extract also inhibited Aspergillus growth on bread slices and delayed colony formation but adversely affected the sensory characteristics of bread. Cinnamon extract at 32 mg/ml not only delayed fungal growth, but also improved bread shelf life and delayed its staling. Moreover, 32mg/ml of extract ...
Introduction: Diabetes is one of the most important metabolic diseases worldwide. Wound infection... more Introduction: Diabetes is one of the most important metabolic diseases worldwide. Wound infections due to antibiotic resistant bacteria can cause lower limbs ulceration and amputation in diabetic patients.The present study was performed with the aim of the evaluation of antibacterial effects of cellulose disc from kombuchaon bacteria isolated from diabetic foot ulcers. Materials and Methods: In this descriptive-analytical study,bacterial were isolated from diabetic wounds and identified based on biochemical and molecular characterization. Then the antibacterial effect of Kombucha cellulose layer wasevaluated on the isolates using disc diffusion (qualitative) and agar dilution (quantitative) methods, and the data was statisticaly analyzed. Results: The most frequency of pathogenic bacteria that isolated in the present study from diabetic wounds were included 56% Escherichia coli (E.coli), 22% Enterobacter cloacae (E. cloacae), 6% Citrobacter diversus (C. diversus), 4% for each of Ent...
Introduction Staphylococcus aureus and Staphylococcus epidermidis are widely recognized as ubiqui... more Introduction Staphylococcus aureus and Staphylococcus epidermidis are widely recognized as ubiquitous and pathogenic bacteria implicated in hospital-acquired infections on a global scale (1-3). S. aureus has been observed to colonize on the skin, particularly when damaged, as well as in various other areas including the perineum, vagina, armpit, navel of neonates, and oropharynx (4, 5). This gram-positive bacterium represents a significant causative agent in the domain of healthcare-associated infections (6). Methicillin-resistant S. aureus (MRSA) is a strain of S. aureus that is resistant to methicillin and other beta-lactam antibiotics. Methicillinresistant S. aureus (MRSA) constitutes a grave concern in the context of hospital-acquired infections, owing to its opportunistic nature, and exacerbates the complexity of treating infections caused by this bacterium (7,8). One of the contributory factors for the resistance of the bacteria to methicillin is the indiscriminate administration of antibiotics, particularly beta-lactam antibiotics. This may be attributed to the inadequate and uninformed
International Journal of Molecular and Clinical Microbiology, 2014
ARTICLE INFO ABSTRACT Article history: Received 3 February 2014 Accepted 27 March 2014 Available ... more ARTICLE INFO ABSTRACT Article history: Received 3 February 2014 Accepted 27 March 2014 Available online 1 June 2014 The use of herbal products in the prevention of the growth of pathogens have been widely studied. Problems in the treatment of infections caused by resistant strains of fungi are indicator of the need for a more in-detailed study of herbal remedies. Artemisia aucheri is used as a medicinal plant with anti-microbial properties, and as an astringent. Plant tissue culture is an important technique for the production of secondary metabolites. The aqueous and methanol extracts (AE and ME) were prepared from the resulting callus. In the agar well diffusion method in a concentration of 2500 mg/ml of AE, the growth inhibition zone diameter (IZD) of clinical isolates of C.albicans was greater than that of fluconazole and both standard strains of C.albicans (PTCC 1167 , 5027) was greater than the positive control. The results of the effect of AE showed that has the greatest effe...
Journal of Herbal Drugs: International Journal on Medicinal Herbs, 2016
Background & Aim: Vaginal disease has always been a great concern of women. Medicinal plants as n... more Background & Aim: Vaginal disease has always been a great concern of women. Medicinal plants as natural resources with fewer side effects can effectively treat these infections given the diversity of these infections, resistance to antibiotics and side effects of existing drugs. The present study aimed to compare antimicrobial effects of methanol extract of Francoeuria Undulata on growth of several gram-positive and gram-negative bacteria causing vaginal infections. Experimental: Francoeuria Undulata as an indigenous Iranian plant was collected in two harvest times (T1 and T2). Methanol extract of aerial parts of the plant was prepared. The effects of various concentrations (62.5, 125, 250 and 500 mg/ml)of methanol extracts were studied onKlebsiella and Enterobacter as gram-negative pathogens. Minimum Inhibitory Concentration (MIC) of bacterial growth and Minimum Bactericidal Concentration (MBC) were determined using macro-dilution method. Data analysis was performed using ANOVA. Re...
Journal of Research in Medical and Dental Science, 2018
In recent years, due to the indiscriminate use of synthetic drugs are irrational drug resistance ... more In recent years, due to the indiscriminate use of synthetic drugs are irrational drug resistance of pathogenic microorganisms is increasing the need for new compounds. The purpose of this study was to investigate the effect of ethanolic and aqueous extracts of Plantago psyllium seed on Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Citrobacter freundii, Enterobacter aerugenes, Pseudomonas aeruginosa and Acinetobacter baumannii, from hospitals (Shariati, Sadoughi, Garazi) and Vahid Medical Laboratory in zarrin shahr were isolated. Also, on the standard of bacteria. Plantago psyllium seed of Agriculture and Natural Resources Research Center of Isfahan was prepared. aqueous and ethanolic extracts were prepared by maceration method. After identification of bacteria, antibiotic resistance was determined by disk diffusion method. Antibacterial effect of ethanolic and aqueous extracts of P. psyllium seed on the growth of multi-drug resistant bacteria by plate diffusion in four...
International journal of Advanced Biological and Biomedical Research, 2013
The objective of this study was to isolation and characterization of Listeria species and determi... more The objective of this study was to isolation and characterization of Listeria species and determines Listeria monocytogenes serotypes in fresh fish, shrimp, crab and lobster in Isfahan and Shahrekord, Iran. From September 2010 to April 2011, a total of 300 samples of fresh fish, shrimp, crab and lobster were obtained from randomly selected retail stores in Isfahan and Shahrekord, Iran. The samples were tested for the presence of Listeria species using a ISO 11290 protocol and then only Listeria monocytogenes isolates identified by bacteriological methods were tested by polymerase chain reaction (PCR) for serotypes detection and presences of virulence associated genes. From the total 300 samples, 23 (10.45%) fresh fish and 1 (2.5%) shrimp samples were positive for Listeria spp., but none of the lobster and crab samples were positive for Listeria species. Listeria monocytogenes only isolated from 17 fish (7.25%) and 1 shrimp (2.5%) samples while L. innocua, L. ivanovii and L. seeliger...
Background: Using smoke from burning donkey dung has been popular in the treatment of many diseas... more Background: Using smoke from burning donkey dung has been popular in the treatment of many diseases in Iran. Objective: This study aimed to investigating the antimicrobial properties of donkey dung smoke on multi-drug resistant (MDR) bacteria isolated from urinary infection. Materials and Methods: First, 300 and 200 urine samples were collected from pregnant and non-pregnant women in Isfahan, Iran. Then in each group, 100 bacterial isolates including Escherichia coli, Klebsiella pneumonia, Proteus vulgaris, Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa, and Staphylococcus saprophyticus were isolated. Antibiotic resistant protocol was determined by antibiogram test. Donkey dung was sterilized, disintegrated, and heated. The smokes were concentrated in n-hexane solvent (65%) and were collected after evaporation of the solvent. Finally, the antibacterial activities of the concentrations of 0.25, 0.5 and 1 mg/mL of the smokes were detected using disk diffusio...
Journal of Gorgan University of Medical Sciences, 2013
Background and Objective: Pollution due to the heavy metals is a problem that may have negative c... more Background and Objective: Pollution due to the heavy metals is a problem that may have negative consequences on the hydrosphere. Identification of microorganisms resistant to heavy metals plays an important role in relation to environmental pollution bioremediation. This study was done to assess the antibiotic resistance in isolated bacteria from urban sewage and copper smeltery industrial wastewater. Materials and Methods: This laboratory study was done on the wastewater samples from urban sewage and copper smeltery in Isfahan-Iran, during 2011-12. Heavy metal resistant microorganisms were isolated and enumerated after serial dilution and culturing on PHG agar plates supplemented with 0.5 mM of each heavy metal. The pattern of resistance was assigned by Minimum Inhibitory Concentration (MIC). Antibiotic resistance toward following medicines Ofloxacine, Penicilline, Sulfometoxasole, Lincomycin, Kanamycin, Streptomycin, Clindamycin, Vancomycin, Cefradin and Neomycin were subsequently...
Antibacterial agents derived from plants are known as suitable alternatives for synthetic pestici... more Antibacterial agents derived from plants are known as suitable alternatives for synthetic pesticides because of their health and the lack of any side effects on plants. This study aims to assess in vitro effect of water, ethanol and methanol extracts of tobacco (Nicotiana plumbaginifolia) leaves on plant pathogenic bacteria and their effect on the control of bacterial infection in tomato plant in the farm conditions. Also, effective compounds of the selected extract were characterized. Water, ethanol and methanol extracts were prepared from the leaves of Nicotiana plumbaginifolia by soaking method. Antibacterial effects of three extracts were determined by agar well diffusion method in 100, 400 and 800 mg/ml concentrations on plant pathogenic bacteria including Xantomonas campestris PTCC1473, Pseudomonas aeruginosa PTCC1181, Pseudomonas syringae PTCC1290, Enterobacter aerogenes PTCC1221, Clavibacter michiganensis PTCC1399, Ralstonia solanacearum PTCC1600 and Gluconacetobacter PTCC17...
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