Conditional and inducible gene targeting using Cre/loxP‐mediated recombination is a powerful reve... more Conditional and inducible gene targeting using Cre/loxP‐mediated recombination is a powerful reverse genetics approach used to study spatiotemporal gene functions in specified cell types. To enable temporal gene manipulation in the melanocyte lineage, we established a novel inducible Cre‐driver mouse line by targeting an all‐in‐one tetracycline/doxycycline (Dox)‐inducible Cre expression cassette into the Pmel locus (PmelP2A‐TetON3G‐TRE3G‐iCre), a gene locus preferentially expressed in pigment cells. By crossing these Cre‐driver mice with a strong Cre‐reporter mouse line, Gt(ROSA)26Sortm9(CAG‐tdTomato)Hze, we show the effectiveness of the PmelP2A‐TetON3G‐TRE3G‐iCre mouse line in facilitating Dox‐inducible Cre/loxP recombination in a wide variety of pigment cell lineages including hair follicle melanocytes and their stem cells. Furthermore, to demonstrate proof of concept, we ablated Notch signaling postnatally in the PmelP2A‐TetON3G‐TRE3G‐iCre mice. In agreement with the previously reported phenotype, induced ablation of Notch signaling in the melanocyte lineage resulted in premature hair graying, demonstrating the utility of the PmelP2A‐TetON3G‐TRE3G‐iCre allele. Therefore, the PmelP2A‐TetON3G‐TRE3G‐iCre mouse line is suitable for assessing gene functions in melanocytes using an in vivo inducible reverse genetics approach. Furthermore, we unexpectedly identified previously unrecognized PMEL‐expressing cells in non‐pigmentary organs in the mice, suggesting unanticipated functions of PMEL other than melanosome formation.
[Histology was performed in the central or primary lymphoid tisues (thymus and bursa of Fabricius... more [Histology was performed in the central or primary lymphoid tisues (thymus and bursa of Fabricius) of the twelve 25-days-old “Kasila” broilers by observation of H & E stained sections in the Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh during the period from March to October 201. Study revealed that the thymus was covered by a thin conective tisue capsule. Numerous fine septa of conective tisue originated from the capsule and divided the organ into many incompletely separated lobules. Each lobule was divided into a peripheral cortex and a central medula. The population of the immunocompetent cels (lymphocytes and immunoglobulin containing plasma cels) in the cortex were denser rather than that of medula of the thymic lobule. The bursa of Fabricius was consisting of long thick mucosal folds (plicae). Numerous folicles filed the lamina propria of each fold and each bursal folicle was composed a peripheral cortex and a central medula. The population of the immunocompetent cels in the cortex of the bursal folicle were denser rather than that of medula of the bursal folicle. The length and width of the thymic lobules were 391.63 ± 25.874 μm and 202.50 ± 15.580 μm, bursal folicles were 594.38 ± 41.205 μm and 464.75 ± 17.721 μm respectively. This study helps to understand the distribution of immunocompetent cels in the central lymphoid organ of broiler.
Conditional and inducible gene targeting using Cre/loxP‐mediated recombination is a powerful reve... more Conditional and inducible gene targeting using Cre/loxP‐mediated recombination is a powerful reverse genetics approach used to study spatiotemporal gene functions in specified cell types. To enable temporal gene manipulation in the melanocyte lineage, we established a novel inducible Cre‐driver mouse line by targeting an all‐in‐one tetracycline/doxycycline (Dox)‐inducible Cre expression cassette into the Pmel locus (PmelP2A‐TetON3G‐TRE3G‐iCre), a gene locus preferentially expressed in pigment cells. By crossing these Cre‐driver mice with a strong Cre‐reporter mouse line, Gt(ROSA)26Sortm9(CAG‐tdTomato)Hze, we show the effectiveness of the PmelP2A‐TetON3G‐TRE3G‐iCre mouse line in facilitating Dox‐inducible Cre/loxP recombination in a wide variety of pigment cell lineages including hair follicle melanocytes and their stem cells. Furthermore, to demonstrate proof of concept, we ablated Notch signaling postnatally in the PmelP2A‐TetON3G‐TRE3G‐iCre mice. In agreement with the previously r...
[Histology was performed in the central or primary lymphoid tisues (thymus and bursa of Fabricius... more [Histology was performed in the central or primary lymphoid tisues (thymus and bursa of Fabricius) of the twelve 25-days-old “Kasila” broilers by observation of H & E stained sections in the Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh during the period from March to October 201. Study revealed that the thymus was covered by a thin conective tisue capsule. Numerous fine septa of conective tisue originated from the capsule and divided the organ into many incompletely separated lobules. Each lobule was divided into a peripheral cortex and a central medula. The population of the immunocompetent cels (lymphocytes and immunoglobulin containing plasma cels) in the cortex were denser rather than that of medula of the thymic lobule. The bursa of Fabricius was consisting of long thick mucosal folds (plicae). Numerous folicles filed the lamina propria of each fold and each bursal folicle was composed a peripheral cortex and a central medula. The population of the immunocompetent cels in the cortex of the bursal folicle were denser rather than that of medula of the bursal folicle. The length and width of the thymic lobules were 391.63 ± 25.874 μm and 202.50 ± 15.580 μm, bursal folicles were 594.38 ± 41.205 μm and 464.75 ± 17.721 μm respectively. This study helps to understand the distribution of immunocompetent cels in the central lymphoid organ of broiler.
Immunohistochemical studies of Harderian gland, cecal tonsil and trachea of various groups of bro... more Immunohistochemical studies of Harderian gland, cecal tonsil and trachea of various groups of broiler chickens and the response of Baby Chick Ranikhet Disease Vaccines (BCRDV) on the mobilization of Igs-cells during postnatal development of organs was investigated in the Dept. of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh. In this study twelve chickens were grouped into vaccinated broilers (D14 and D28) which had received vaccines first at D3 of age and a booster dose given at D13; and non-vaccinated broilers (D1) which had not been vaccinated. In this study, it was observed that the frequency and distribution of Igs-positive cells were higher at D14 and at D28 rather than D1. Among Igs-positive cells, the IgG-positive cells were significantly higher than IgM and IgA-positive cells in the Harderian gland of D14 and D28 groups of chickens, however, in day-old chickens, the frequency of IgM-positive cells in this gland were greater. In the cecal tonsil, the frequency and distribution of IgG-positive cells were significantly higher than IgA- and IgM-positive cells both at D14 and D28 ages of chicken. On the other hand, in day-old chickens, the frequency and distribution of IgA-positive cells were insignificantly greater, followed by IgM and IgG-positive cells. In the trachea, few immunoglobulin-containing plasma cells were distributed in the subepithelial layer. IgM-positive cells were higher followed by IgG and IgA-positive cells in the trachea in D14 and D28 groups of chickens. In the same organ, IgG-positive plasma cells were greater than IgA and IgM-positive cells at one-day old. When the data for Harderian gland, cecal tonsil and trachea were compared statistically, it was observed that Igs-positive cells were statistically more common in cecal tonsils in day old chickens, and with the advancement of age, Igs-positive cells were found more in the Harderian gland. In conclusion, with the advancement of age in chickens the Harderian gland uptake is a function of the cecal tonsil due to its functional atrophy.
Journal of the Bangladesh Agricultural University, 2014
Histomorphological changes of epididymis and ductus deferens in male Black Bengal goat due to ars... more Histomorphological changes of epididymis and ductus deferens in male Black Bengal goat due to arsenic were studied. A total of 12 male Black Bengal goats, in which 6 were collected from arsenic affected areas of Mymensingh district and another 6 were collected from hill tracts of Chittagong which were arsenic free.The goats were sacrificed by piercing carotid artery and the samples (Epididymis and Ductus deferens) were collected immediately. Samples were prepared and stained with haematoxylin and eosin stain technique to study the histology under light microscope. In the morphological study, measurement of length, width, breadth and weight of epididymis and ductus deferens were observed. The gross study revealed that there were slight variations in the gross morphology of epididymis and ductus deferens of arsenic affected Black Bengal goat, but this variation was statistically insignificant. In the histological study, arsenic affected goat showing increased thickness of epididymal c...
Histological studies and development of immunoglobulin containing plasma cells in ileum represent... more Histological studies and development of immunoglobulin containing plasma cells in ileum representing gut-associated-lymphatic tissue of various group of broiler chickens vaccinated with Baby Chick Ranikhet Disease Vaccine (BCRDV) was carried out in the Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh during January to October, 2011. A total of 24 day-old broiler chickens of both sexes were divided into two groups such as vaccinated and control. Vaccinated group of broiler chickens were vaccinated at third day through ocular route. It was observed that the villi of ileum were long and slender, lined by simple columnar cells with numerous goblet cells. Scattered lymphocytes and unorganized lymphatic nodules were present in the lamina propria and core of the villi. The frequency and distribution of lymphocytes in the lamina propria and intraepithelial lymphocytes were increased with concentration in vaccinated group. The length and width of mucosa wer...
Conditional and inducible gene targeting using Cre/loxP‐mediated recombination is a powerful reve... more Conditional and inducible gene targeting using Cre/loxP‐mediated recombination is a powerful reverse genetics approach used to study spatiotemporal gene functions in specified cell types. To enable temporal gene manipulation in the melanocyte lineage, we established a novel inducible Cre‐driver mouse line by targeting an all‐in‐one tetracycline/doxycycline (Dox)‐inducible Cre expression cassette into the Pmel locus (PmelP2A‐TetON3G‐TRE3G‐iCre), a gene locus preferentially expressed in pigment cells. By crossing these Cre‐driver mice with a strong Cre‐reporter mouse line, Gt(ROSA)26Sortm9(CAG‐tdTomato)Hze, we show the effectiveness of the PmelP2A‐TetON3G‐TRE3G‐iCre mouse line in facilitating Dox‐inducible Cre/loxP recombination in a wide variety of pigment cell lineages including hair follicle melanocytes and their stem cells. Furthermore, to demonstrate proof of concept, we ablated Notch signaling postnatally in the PmelP2A‐TetON3G‐TRE3G‐iCre mice. In agreement with the previously reported phenotype, induced ablation of Notch signaling in the melanocyte lineage resulted in premature hair graying, demonstrating the utility of the PmelP2A‐TetON3G‐TRE3G‐iCre allele. Therefore, the PmelP2A‐TetON3G‐TRE3G‐iCre mouse line is suitable for assessing gene functions in melanocytes using an in vivo inducible reverse genetics approach. Furthermore, we unexpectedly identified previously unrecognized PMEL‐expressing cells in non‐pigmentary organs in the mice, suggesting unanticipated functions of PMEL other than melanosome formation.
[Histology was performed in the central or primary lymphoid tisues (thymus and bursa of Fabricius... more [Histology was performed in the central or primary lymphoid tisues (thymus and bursa of Fabricius) of the twelve 25-days-old “Kasila” broilers by observation of H & E stained sections in the Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh during the period from March to October 201. Study revealed that the thymus was covered by a thin conective tisue capsule. Numerous fine septa of conective tisue originated from the capsule and divided the organ into many incompletely separated lobules. Each lobule was divided into a peripheral cortex and a central medula. The population of the immunocompetent cels (lymphocytes and immunoglobulin containing plasma cels) in the cortex were denser rather than that of medula of the thymic lobule. The bursa of Fabricius was consisting of long thick mucosal folds (plicae). Numerous folicles filed the lamina propria of each fold and each bursal folicle was composed a peripheral cortex and a central medula. The population of the immunocompetent cels in the cortex of the bursal folicle were denser rather than that of medula of the bursal folicle. The length and width of the thymic lobules were 391.63 ± 25.874 μm and 202.50 ± 15.580 μm, bursal folicles were 594.38 ± 41.205 μm and 464.75 ± 17.721 μm respectively. This study helps to understand the distribution of immunocompetent cels in the central lymphoid organ of broiler.
Conditional and inducible gene targeting using Cre/loxP‐mediated recombination is a powerful reve... more Conditional and inducible gene targeting using Cre/loxP‐mediated recombination is a powerful reverse genetics approach used to study spatiotemporal gene functions in specified cell types. To enable temporal gene manipulation in the melanocyte lineage, we established a novel inducible Cre‐driver mouse line by targeting an all‐in‐one tetracycline/doxycycline (Dox)‐inducible Cre expression cassette into the Pmel locus (PmelP2A‐TetON3G‐TRE3G‐iCre), a gene locus preferentially expressed in pigment cells. By crossing these Cre‐driver mice with a strong Cre‐reporter mouse line, Gt(ROSA)26Sortm9(CAG‐tdTomato)Hze, we show the effectiveness of the PmelP2A‐TetON3G‐TRE3G‐iCre mouse line in facilitating Dox‐inducible Cre/loxP recombination in a wide variety of pigment cell lineages including hair follicle melanocytes and their stem cells. Furthermore, to demonstrate proof of concept, we ablated Notch signaling postnatally in the PmelP2A‐TetON3G‐TRE3G‐iCre mice. In agreement with the previously r...
[Histology was performed in the central or primary lymphoid tisues (thymus and bursa of Fabricius... more [Histology was performed in the central or primary lymphoid tisues (thymus and bursa of Fabricius) of the twelve 25-days-old “Kasila” broilers by observation of H & E stained sections in the Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh during the period from March to October 201. Study revealed that the thymus was covered by a thin conective tisue capsule. Numerous fine septa of conective tisue originated from the capsule and divided the organ into many incompletely separated lobules. Each lobule was divided into a peripheral cortex and a central medula. The population of the immunocompetent cels (lymphocytes and immunoglobulin containing plasma cels) in the cortex were denser rather than that of medula of the thymic lobule. The bursa of Fabricius was consisting of long thick mucosal folds (plicae). Numerous folicles filed the lamina propria of each fold and each bursal folicle was composed a peripheral cortex and a central medula. The population of the immunocompetent cels in the cortex of the bursal folicle were denser rather than that of medula of the bursal folicle. The length and width of the thymic lobules were 391.63 ± 25.874 μm and 202.50 ± 15.580 μm, bursal folicles were 594.38 ± 41.205 μm and 464.75 ± 17.721 μm respectively. This study helps to understand the distribution of immunocompetent cels in the central lymphoid organ of broiler.
Immunohistochemical studies of Harderian gland, cecal tonsil and trachea of various groups of bro... more Immunohistochemical studies of Harderian gland, cecal tonsil and trachea of various groups of broiler chickens and the response of Baby Chick Ranikhet Disease Vaccines (BCRDV) on the mobilization of Igs-cells during postnatal development of organs was investigated in the Dept. of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh. In this study twelve chickens were grouped into vaccinated broilers (D14 and D28) which had received vaccines first at D3 of age and a booster dose given at D13; and non-vaccinated broilers (D1) which had not been vaccinated. In this study, it was observed that the frequency and distribution of Igs-positive cells were higher at D14 and at D28 rather than D1. Among Igs-positive cells, the IgG-positive cells were significantly higher than IgM and IgA-positive cells in the Harderian gland of D14 and D28 groups of chickens, however, in day-old chickens, the frequency of IgM-positive cells in this gland were greater. In the cecal tonsil, the frequency and distribution of IgG-positive cells were significantly higher than IgA- and IgM-positive cells both at D14 and D28 ages of chicken. On the other hand, in day-old chickens, the frequency and distribution of IgA-positive cells were insignificantly greater, followed by IgM and IgG-positive cells. In the trachea, few immunoglobulin-containing plasma cells were distributed in the subepithelial layer. IgM-positive cells were higher followed by IgG and IgA-positive cells in the trachea in D14 and D28 groups of chickens. In the same organ, IgG-positive plasma cells were greater than IgA and IgM-positive cells at one-day old. When the data for Harderian gland, cecal tonsil and trachea were compared statistically, it was observed that Igs-positive cells were statistically more common in cecal tonsils in day old chickens, and with the advancement of age, Igs-positive cells were found more in the Harderian gland. In conclusion, with the advancement of age in chickens the Harderian gland uptake is a function of the cecal tonsil due to its functional atrophy.
Journal of the Bangladesh Agricultural University, 2014
Histomorphological changes of epididymis and ductus deferens in male Black Bengal goat due to ars... more Histomorphological changes of epididymis and ductus deferens in male Black Bengal goat due to arsenic were studied. A total of 12 male Black Bengal goats, in which 6 were collected from arsenic affected areas of Mymensingh district and another 6 were collected from hill tracts of Chittagong which were arsenic free.The goats were sacrificed by piercing carotid artery and the samples (Epididymis and Ductus deferens) were collected immediately. Samples were prepared and stained with haematoxylin and eosin stain technique to study the histology under light microscope. In the morphological study, measurement of length, width, breadth and weight of epididymis and ductus deferens were observed. The gross study revealed that there were slight variations in the gross morphology of epididymis and ductus deferens of arsenic affected Black Bengal goat, but this variation was statistically insignificant. In the histological study, arsenic affected goat showing increased thickness of epididymal c...
Histological studies and development of immunoglobulin containing plasma cells in ileum represent... more Histological studies and development of immunoglobulin containing plasma cells in ileum representing gut-associated-lymphatic tissue of various group of broiler chickens vaccinated with Baby Chick Ranikhet Disease Vaccine (BCRDV) was carried out in the Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh during January to October, 2011. A total of 24 day-old broiler chickens of both sexes were divided into two groups such as vaccinated and control. Vaccinated group of broiler chickens were vaccinated at third day through ocular route. It was observed that the villi of ileum were long and slender, lined by simple columnar cells with numerous goblet cells. Scattered lymphocytes and unorganized lymphatic nodules were present in the lamina propria and core of the villi. The frequency and distribution of lymphocytes in the lamina propria and intraepithelial lymphocytes were increased with concentration in vaccinated group. The length and width of mucosa wer...
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