We describe updated information on the various vaccines available over the last two decades, alon... more We describe updated information on the various vaccines available over the last two decades, along with recent progress in developing 63 diverse vaccines against SARS-CoV-2.
Coliforms and Escherichia coli represent the fecal contamination in drinking water and hence its ... more Coliforms and Escherichia coli represent the fecal contamination in drinking water and hence its potability. The present study estimates uidA and lacZ genes as distinctive biomarkers for rapid and efficient detection of contaminated water resources. In total, 39 environmental water samples were collected in and around the metropolitan city of Chennai, India. The results were compared with culture-based method using selective medium. The study shows that the performance and amplification efficiency of uidA and lacZ are 99.6% and 103.4% respectively. The sensitivity of the qPCR method in selectively identifying E. coli was 71.4% in a total of 92.3% coliform contamination. The conventional method showed that 10 samples were positive for E. coli and 12 samples positive for coliforms from a total of 39 samples. A lower positive predictive value of the biomarkers observed is due to the insignificant association between the two methods and is determined to be 59.0% and 66.6% for E. coli an...
An efficient approach for the synthesis of pyrazoline/pyrazole-tethered pyridinyl methanones is d... more An efficient approach for the synthesis of pyrazoline/pyrazole-tethered pyridinyl methanones is described via a one-pot, stepwise, sequential methodology using chalcones and pyridine-4-carbohydrazide as substrates through a Michael addition followed by cyclization. The reaction proceeds via a catalyst-, solvent-, work-up-, and column-chromatography-free method under melt conditions to provide the pyrazolines in short reaction times with high atom efficiency.
Stem and bark of the tree Terminalia arjuna Wight &Arn. (Combretaceae) has been documented to exh... more Stem and bark of the tree Terminalia arjuna Wight &Arn. (Combretaceae) has been documented to exhibit therapeutic properties like cardiotonic, anticancer, antiviral, antibacterial, antifungal, hypocholsteremic, hypolipidemic and anti-coagulant. In previous studies, ethanolic extract of T.arjunabark effectively inhibited catalase activity along with demonstration of DPPH radical scavenging activity. Further,four known oleananetriterpenoids type compounds viz., oleanolic acid, arjunolic acid, arjunolitin, arjunetin were isolated from ethaolic bark extract and the structures of which were elucidated using 1 H, 13C NMR, HR-ESIMS, IR and compared with literature data. Of the various compounds, Arjunetin showed significant inhibition of catalase activity as compared to the other compounds and most probably conferring antibacterial and antiviral property of the extract. In the present study, considering the currently on going viral pandemic of SARS-CoV-2 and the need for an effective antiv...
ABSTRACTDifferent types of α-synuclein and non-β-amyloid component (NAC) peptides have been shown... more ABSTRACTDifferent types of α-synuclein and non-β-amyloid component (NAC) peptides have been shown to induce cell death, with varying degree of toxicity, in various in vitro experiments. Oxidative stress has also been associated and proved to be involved in the pathogenesis of neuronal cell death in Parkinson’s disease. Oxidative stress has been shown to accelerate the aggregation of α-synuclein in vitro and in resent studies α-synuclein has been shown to increase oxidative stress. Thus it seems like a vicious cycle, one promoting the other.In this present work we have modeled the α-synuclein pathway to increase cytoplasmic Dopamine concentration, and thereby increasing the Reactive Oxygen Species (ROS) level of the cell, which consequently results in cell death. This model relates the α-synuclein concentration with the fractional cell survival and provides insight of crucial reaction(s) of α-synuclein which promote cell death. It predicts the toxicity of the type of α-synuclein and ...
Leukotriene and prostaglandin pathways are controlled by the enzymes, LOX and COX/mPGES1 respecti... more Leukotriene and prostaglandin pathways are controlled by the enzymes, LOX and COX/mPGES1 respectively and are responsible for inflammatory responses.
We describe updated information on the various vaccines available over the last two decades, alon... more We describe updated information on the various vaccines available over the last two decades, along with recent progress in developing 63 diverse vaccines against SARS-CoV-2.
Coliforms and Escherichia coli represent the fecal contamination in drinking water and hence its ... more Coliforms and Escherichia coli represent the fecal contamination in drinking water and hence its potability. The present study estimates uidA and lacZ genes as distinctive biomarkers for rapid and efficient detection of contaminated water resources. In total, 39 environmental water samples were collected in and around the metropolitan city of Chennai, India. The results were compared with culture-based method using selective medium. The study shows that the performance and amplification efficiency of uidA and lacZ are 99.6% and 103.4% respectively. The sensitivity of the qPCR method in selectively identifying E. coli was 71.4% in a total of 92.3% coliform contamination. The conventional method showed that 10 samples were positive for E. coli and 12 samples positive for coliforms from a total of 39 samples. A lower positive predictive value of the biomarkers observed is due to the insignificant association between the two methods and is determined to be 59.0% and 66.6% for E. coli an...
An efficient approach for the synthesis of pyrazoline/pyrazole-tethered pyridinyl methanones is d... more An efficient approach for the synthesis of pyrazoline/pyrazole-tethered pyridinyl methanones is described via a one-pot, stepwise, sequential methodology using chalcones and pyridine-4-carbohydrazide as substrates through a Michael addition followed by cyclization. The reaction proceeds via a catalyst-, solvent-, work-up-, and column-chromatography-free method under melt conditions to provide the pyrazolines in short reaction times with high atom efficiency.
Stem and bark of the tree Terminalia arjuna Wight &Arn. (Combretaceae) has been documented to exh... more Stem and bark of the tree Terminalia arjuna Wight &Arn. (Combretaceae) has been documented to exhibit therapeutic properties like cardiotonic, anticancer, antiviral, antibacterial, antifungal, hypocholsteremic, hypolipidemic and anti-coagulant. In previous studies, ethanolic extract of T.arjunabark effectively inhibited catalase activity along with demonstration of DPPH radical scavenging activity. Further,four known oleananetriterpenoids type compounds viz., oleanolic acid, arjunolic acid, arjunolitin, arjunetin were isolated from ethaolic bark extract and the structures of which were elucidated using 1 H, 13C NMR, HR-ESIMS, IR and compared with literature data. Of the various compounds, Arjunetin showed significant inhibition of catalase activity as compared to the other compounds and most probably conferring antibacterial and antiviral property of the extract. In the present study, considering the currently on going viral pandemic of SARS-CoV-2 and the need for an effective antiv...
ABSTRACTDifferent types of α-synuclein and non-β-amyloid component (NAC) peptides have been shown... more ABSTRACTDifferent types of α-synuclein and non-β-amyloid component (NAC) peptides have been shown to induce cell death, with varying degree of toxicity, in various in vitro experiments. Oxidative stress has also been associated and proved to be involved in the pathogenesis of neuronal cell death in Parkinson’s disease. Oxidative stress has been shown to accelerate the aggregation of α-synuclein in vitro and in resent studies α-synuclein has been shown to increase oxidative stress. Thus it seems like a vicious cycle, one promoting the other.In this present work we have modeled the α-synuclein pathway to increase cytoplasmic Dopamine concentration, and thereby increasing the Reactive Oxygen Species (ROS) level of the cell, which consequently results in cell death. This model relates the α-synuclein concentration with the fractional cell survival and provides insight of crucial reaction(s) of α-synuclein which promote cell death. It predicts the toxicity of the type of α-synuclein and ...
Leukotriene and prostaglandin pathways are controlled by the enzymes, LOX and COX/mPGES1 respecti... more Leukotriene and prostaglandin pathways are controlled by the enzymes, LOX and COX/mPGES1 respectively and are responsible for inflammatory responses.
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Papers by Mukesh Doble