Based on our hypothesis for existing microbiota of wall-deficient variants (L-forms) in human blo... more Based on our hypothesis for existing microbiota of wall-deficient variants (L-forms) in human blood, we created an innovative methodology, which allowed for the development of L-form populations from blood of all investigated people. In contrast to healthy controls, blood L-forms from autistic children and their mothers converted under appropriate conditions of cultivation into detectable opportunistic bacteria and fungi, а process demonstrated by light and transmission electron microscopy. It can be distinguished into two types of states – “eubiotic” blood microbiota in healthy individuals, and “dysbiotic” in autistic children and their mothers. Remarkably, the unifying finding for autistic children and their mothers was the presence in blood of wall-free variants from life-cycle of filamentous fungi. Increased specific IgG, IgM and IgA, together with typical mold growth were a decisive argument for proven presence of Aspergillus fumigatus in almost all of the autistic children. As...
Cigarette smoking alters the presence and function of alveolar macrophages. It has been speculate... more Cigarette smoking alters the presence and function of alveolar macrophages. It has been speculated that these cigarette smoke-induced alterations contribute to the depressed pulmonary defense mechanisms commonly demonstrated in smokers. Studies of the phagocytic and bactericidal activities of alveolar macrophages from smokers and nonsmokers have yielded conflicting results. We tested whether alveolar macrophages from normal nonsmokers versus normal smokers differed in their ability to phagocytose and to kill the facultative intracellular bacterium Listeria monocytogenes. No significant differences in phagocytosis between nonsmokers and smokers were found. The alveolar macrophages from nonsmokers, however, killed Listeria, whereas those from smokers had no bactericidal or bacteriostatic activity. Thus, these data demonstrate that alveolar macrophages from normal smokers are able to phagocytose Listeria but express a selective functional deficiency in their ability to kill this facultative intracellular bacteria and, therefore, imply a defect in the immunoregulation of alveolar macrophages in smokers.
Composite fibrous materials are prepared from poly(ethylene oxide) (PEO) and beeswax (BW) by sing... more Composite fibrous materials are prepared from poly(ethylene oxide) (PEO) and beeswax (BW) by single-spinneret electrospinning using chloroform as a common solvent. The obtained fibers have core-sheath-like structure, as evidenced by the water contact angle values and corroborated by the results on the elemental composition of the fibers surface determined by X-ray photoelectron spectroscopy (XPS) and by analyses with scanning electron microscopy (SEM) of fibers before and after selective extraction of PEO or of BW. Furthermore, the core-sheath-like structure is proven by transmission electron microcopy (TEM). This is attributed to self-assembly of BW molecules on the surface of the formed fibers driven by the incompatibility between PEO and BW. 5-Nitro-8-hydroxyquinoline (NQ) is embedded as a model drug with antibacterial, antifungal, and anticancer properties in the PEO/BW fibrous materials. XPS analyses reveal that NQ is present on the surface of the PEO/BW/NQ materials. Using a purposely designed cell for fixation of the fibrous materials the NQ release in phosphate buffer solution with рН 7.4 is followed. The new PEO/BW/NQ fibrous materials exhibit antibacterial activity against S. aureus and E. coli, antifungal effect against C. albicans, and selective anticancer activity against HeLa (human cervical adenocarcinoma cells) and SH-4 (human melanoma cells) cell lines. This article is protected by copyright. All rights reserved.
Novel eco-friendly fibrous materials with complex activities from cellulose acetate and cellulose... more Novel eco-friendly fibrous materials with complex activities from cellulose acetate and cellulose acetate/polyethylene glycol (CA,PEG) containing 5-chloro-8-hydroxyquinoline as a model drug were obtained by electrospinning. Several methods, including scanning electron microscopy, X-ray diffraction analysis, ultraviolet-visible spectroscopy, water contact angle measurements, and mechanical tests, were utilized to characterize the obtained materials. The incorporation of PEG into the fibers facilitated the drug release. The amounts of the released drug from CA/5-Cl8Q and CA,PEG/5-Cl8Q were 78 ± 3.38% and 86 ± 3.02%, respectively (for 175 min). The antibacterial and antifungal activities of the obtained materials were studied. The measured zones of inhibition of CA/5-Cl8Q and CA,PEG/5-Cl8Q mats were 4.0 ± 0.18 and 4.5 ± 0.2 cm against S. aureus and around 4.0 ± 0.15 and 4.1 ± 0.22 cm against E. coli, respectively. The complete inhibition of the C. albicans growth was detected. The cyto...
Novel poly(vinyl alcohol) (PVA)/chitosan (Ch)-based fibrous materials containing an ionizable mod... more Novel poly(vinyl alcohol) (PVA)/chitosan (Ch)-based fibrous materials containing an ionizable model drug, 8-hydroxyquinoline-5-sulfonic acid (SQ), were successfully fabricated by electrospinning. Complexes between the components of the crosslinked PVA/Ch/SQ mats and Cu2+ and Fe3+ ions were formed. The coordination of these ions in the mats was examined by electron paramagnetic resonance spectroscopy (EPR). The microbiological screening against S. aureus and C. albicans revealed that both the incorporation of SQ in the mats and the complexation with Cu2+ and Fe3+ imparted to these materials antibacterial and antifungal activities. Moreover, the SQ-containing mats and their complexes displayed good cytotoxicity against human cervical HeLa tumor cells. The most prominent was the cytotoxicity of the Cu2+ complex of the mats. The combined antibacterial, antifungal and in vitro antitumor activities render these novel materials promising candidates for wound dressing applications and for a...
Journal of materials science. Materials in medicine, Jan 23, 2017
Curcumin (Curc) exhibits anti-inflammatory, antibacterial and antitumor activity. However, its cl... more Curcumin (Curc) exhibits anti-inflammatory, antibacterial and antitumor activity. However, its clinical application is limited by its poor bioavailability related to its extremely low water solubility. Novel materials allowing enhanced release of Curc in aqueous medium were obtained. The new materials consisted of electrospun fibers from cellulose acetate (CA) (mean fiber diameter ca. 780 nm ± 110 nm) with electrosprayed Curc/polyvinylpyrrolidone (Curc/PVP) particles. Scanning electron microscopy (SEM) showed that separated and evenly distributed particles of Curc/PVP were deposited on the surface of the mats and on the inner layers of the mat. X-ray diffraction studies showed that Curc was in amorphous state. In vitro studies demonstrated that Curc release was facilitated from Curc/PVP-on-CA mats (ca. 78% for 24 h) compared with the materials in which Curc was incorporated in CA fibers (17% for 24 h). Moreover, the curcumin-containing materials exhibited antibacterial activity agai...
Tuberculosis (TB) infects a significant proportion of the world population and constitutes a majo... more Tuberculosis (TB) infects a significant proportion of the world population and constitutes a major public health problem, particularly, in the developing regions. A reemergence of TB accompanied by an increasing number of drug resistant Mycobacterium tuberculosis strains, including many resistant to multiple drugs, has been noted since the mid-1980s. In Bulgaria, the rate of multidrug-resistant tuberculosis (MDR-TB) was estimated to be 10.7% among newly diagnosed TB patients in 2006 that is much higher as compared to the neighboring countries. An increasing rate of MDR-TB implies importance of surveillance of resistance and its fast detection. Characterization of molecular basis of drug resistance in a survey area is a first step prior to implementation of such methods. Here, we present data on molecular basis of drug-resistance in M. tuberculosis strains currently circulating in Bulgaria. We also compared distribution of drug resistance mutations within the main genotypic clusters ...
ABSTRACT Polyelectrolyte complexes (PECs) were prepared from N,N,N-trimethylchitosan iodide (TMCh... more ABSTRACT Polyelectrolyte complexes (PECs) were prepared from N,N,N-trimethylchitosan iodide (TMCh) of different molar mass and a weak polyacid-poly(acrylic acid) (PAA) or a strong polyacid-poly(2-acrylamido-2-methylpropanesulfonic acid) (PAMPS). The quaternization of the amino groups of chitosan enabled the formation of water-insoluble PECs in a broad pH range—from 3 to 12 and from 1 to 12 for TMCh/PAA and TMCh/PAMPS, respectively. Whereas the stoichiometry of the TMCh/PAA complex was pH dependant, the stoichiometry of the TMCh/PAMPS complex did not depend on pH. The stoichiometry and the yield of the complexes were influenced by the molar mass of TMCh. PEC nanoparticles were produced by mixing dilute solutions of the oppositely charged polyelectrolytes as revealed by dynamic light scattering analyses. The size of the particles was in the range of 135–924 nm and depended on the polyelectrolyte molar mass, the initial polyelectrolyte concentration, and the molar fraction of the TMCh units. Microbiological screening against Staphylococcus aureus and Escherichia coli revealed that PECs between TMCh and PAA or PAMPS have a good antibacterial effect, which is more slowly pronounced than that of the starting TMCh of different molar mass.
Based on our hypothesis for existing microbiota of wall-deficient variants (L-forms) in human blo... more Based on our hypothesis for existing microbiota of wall-deficient variants (L-forms) in human blood, we created an innovative methodology, which allowed for the development of L-form populations from blood of all investigated people. In contrast to healthy controls, blood L-forms from autistic children and their mothers converted under appropriate conditions of cultivation into detectable opportunistic bacteria and fungi, а process demonstrated by light and transmission electron microscopy. It can be distinguished into two types of states – “eubiotic” blood microbiota in healthy individuals, and “dysbiotic” in autistic children and their mothers. Remarkably, the unifying finding for autistic children and their mothers was the presence in blood of wall-free variants from life-cycle of filamentous fungi. Increased specific IgG, IgM and IgA, together with typical mold growth were a decisive argument for proven presence of Aspergillus fumigatus in almost all of the autistic children. As...
Cigarette smoking alters the presence and function of alveolar macrophages. It has been speculate... more Cigarette smoking alters the presence and function of alveolar macrophages. It has been speculated that these cigarette smoke-induced alterations contribute to the depressed pulmonary defense mechanisms commonly demonstrated in smokers. Studies of the phagocytic and bactericidal activities of alveolar macrophages from smokers and nonsmokers have yielded conflicting results. We tested whether alveolar macrophages from normal nonsmokers versus normal smokers differed in their ability to phagocytose and to kill the facultative intracellular bacterium Listeria monocytogenes. No significant differences in phagocytosis between nonsmokers and smokers were found. The alveolar macrophages from nonsmokers, however, killed Listeria, whereas those from smokers had no bactericidal or bacteriostatic activity. Thus, these data demonstrate that alveolar macrophages from normal smokers are able to phagocytose Listeria but express a selective functional deficiency in their ability to kill this facultative intracellular bacteria and, therefore, imply a defect in the immunoregulation of alveolar macrophages in smokers.
Composite fibrous materials are prepared from poly(ethylene oxide) (PEO) and beeswax (BW) by sing... more Composite fibrous materials are prepared from poly(ethylene oxide) (PEO) and beeswax (BW) by single-spinneret electrospinning using chloroform as a common solvent. The obtained fibers have core-sheath-like structure, as evidenced by the water contact angle values and corroborated by the results on the elemental composition of the fibers surface determined by X-ray photoelectron spectroscopy (XPS) and by analyses with scanning electron microscopy (SEM) of fibers before and after selective extraction of PEO or of BW. Furthermore, the core-sheath-like structure is proven by transmission electron microcopy (TEM). This is attributed to self-assembly of BW molecules on the surface of the formed fibers driven by the incompatibility between PEO and BW. 5-Nitro-8-hydroxyquinoline (NQ) is embedded as a model drug with antibacterial, antifungal, and anticancer properties in the PEO/BW fibrous materials. XPS analyses reveal that NQ is present on the surface of the PEO/BW/NQ materials. Using a purposely designed cell for fixation of the fibrous materials the NQ release in phosphate buffer solution with рН 7.4 is followed. The new PEO/BW/NQ fibrous materials exhibit antibacterial activity against S. aureus and E. coli, antifungal effect against C. albicans, and selective anticancer activity against HeLa (human cervical adenocarcinoma cells) and SH-4 (human melanoma cells) cell lines. This article is protected by copyright. All rights reserved.
Novel eco-friendly fibrous materials with complex activities from cellulose acetate and cellulose... more Novel eco-friendly fibrous materials with complex activities from cellulose acetate and cellulose acetate/polyethylene glycol (CA,PEG) containing 5-chloro-8-hydroxyquinoline as a model drug were obtained by electrospinning. Several methods, including scanning electron microscopy, X-ray diffraction analysis, ultraviolet-visible spectroscopy, water contact angle measurements, and mechanical tests, were utilized to characterize the obtained materials. The incorporation of PEG into the fibers facilitated the drug release. The amounts of the released drug from CA/5-Cl8Q and CA,PEG/5-Cl8Q were 78 ± 3.38% and 86 ± 3.02%, respectively (for 175 min). The antibacterial and antifungal activities of the obtained materials were studied. The measured zones of inhibition of CA/5-Cl8Q and CA,PEG/5-Cl8Q mats were 4.0 ± 0.18 and 4.5 ± 0.2 cm against S. aureus and around 4.0 ± 0.15 and 4.1 ± 0.22 cm against E. coli, respectively. The complete inhibition of the C. albicans growth was detected. The cyto...
Novel poly(vinyl alcohol) (PVA)/chitosan (Ch)-based fibrous materials containing an ionizable mod... more Novel poly(vinyl alcohol) (PVA)/chitosan (Ch)-based fibrous materials containing an ionizable model drug, 8-hydroxyquinoline-5-sulfonic acid (SQ), were successfully fabricated by electrospinning. Complexes between the components of the crosslinked PVA/Ch/SQ mats and Cu2+ and Fe3+ ions were formed. The coordination of these ions in the mats was examined by electron paramagnetic resonance spectroscopy (EPR). The microbiological screening against S. aureus and C. albicans revealed that both the incorporation of SQ in the mats and the complexation with Cu2+ and Fe3+ imparted to these materials antibacterial and antifungal activities. Moreover, the SQ-containing mats and their complexes displayed good cytotoxicity against human cervical HeLa tumor cells. The most prominent was the cytotoxicity of the Cu2+ complex of the mats. The combined antibacterial, antifungal and in vitro antitumor activities render these novel materials promising candidates for wound dressing applications and for a...
Journal of materials science. Materials in medicine, Jan 23, 2017
Curcumin (Curc) exhibits anti-inflammatory, antibacterial and antitumor activity. However, its cl... more Curcumin (Curc) exhibits anti-inflammatory, antibacterial and antitumor activity. However, its clinical application is limited by its poor bioavailability related to its extremely low water solubility. Novel materials allowing enhanced release of Curc in aqueous medium were obtained. The new materials consisted of electrospun fibers from cellulose acetate (CA) (mean fiber diameter ca. 780 nm ± 110 nm) with electrosprayed Curc/polyvinylpyrrolidone (Curc/PVP) particles. Scanning electron microscopy (SEM) showed that separated and evenly distributed particles of Curc/PVP were deposited on the surface of the mats and on the inner layers of the mat. X-ray diffraction studies showed that Curc was in amorphous state. In vitro studies demonstrated that Curc release was facilitated from Curc/PVP-on-CA mats (ca. 78% for 24 h) compared with the materials in which Curc was incorporated in CA fibers (17% for 24 h). Moreover, the curcumin-containing materials exhibited antibacterial activity agai...
Tuberculosis (TB) infects a significant proportion of the world population and constitutes a majo... more Tuberculosis (TB) infects a significant proportion of the world population and constitutes a major public health problem, particularly, in the developing regions. A reemergence of TB accompanied by an increasing number of drug resistant Mycobacterium tuberculosis strains, including many resistant to multiple drugs, has been noted since the mid-1980s. In Bulgaria, the rate of multidrug-resistant tuberculosis (MDR-TB) was estimated to be 10.7% among newly diagnosed TB patients in 2006 that is much higher as compared to the neighboring countries. An increasing rate of MDR-TB implies importance of surveillance of resistance and its fast detection. Characterization of molecular basis of drug resistance in a survey area is a first step prior to implementation of such methods. Here, we present data on molecular basis of drug-resistance in M. tuberculosis strains currently circulating in Bulgaria. We also compared distribution of drug resistance mutations within the main genotypic clusters ...
ABSTRACT Polyelectrolyte complexes (PECs) were prepared from N,N,N-trimethylchitosan iodide (TMCh... more ABSTRACT Polyelectrolyte complexes (PECs) were prepared from N,N,N-trimethylchitosan iodide (TMCh) of different molar mass and a weak polyacid-poly(acrylic acid) (PAA) or a strong polyacid-poly(2-acrylamido-2-methylpropanesulfonic acid) (PAMPS). The quaternization of the amino groups of chitosan enabled the formation of water-insoluble PECs in a broad pH range—from 3 to 12 and from 1 to 12 for TMCh/PAA and TMCh/PAMPS, respectively. Whereas the stoichiometry of the TMCh/PAA complex was pH dependant, the stoichiometry of the TMCh/PAMPS complex did not depend on pH. The stoichiometry and the yield of the complexes were influenced by the molar mass of TMCh. PEC nanoparticles were produced by mixing dilute solutions of the oppositely charged polyelectrolytes as revealed by dynamic light scattering analyses. The size of the particles was in the range of 135–924 nm and depended on the polyelectrolyte molar mass, the initial polyelectrolyte concentration, and the molar fraction of the TMCh units. Microbiological screening against Staphylococcus aureus and Escherichia coli revealed that PECs between TMCh and PAA or PAMPS have a good antibacterial effect, which is more slowly pronounced than that of the starting TMCh of different molar mass.
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