Fortyfour years of teaching and research in Virology. Holding position of Professor for 33 yrs. Head of Institution for 11 yrs. Area of interest Molecular Virology, Vaccinology, Genetic Engineering Address: Uttar Pradesh
Wilson's disease is a hereditary defect in hepatic copper metabolism, causing hepatic, ne... more Wilson's disease is a hereditary defect in hepatic copper metabolism, causing hepatic, neurological and/or psychiatric manifestations. For patients with severe disease, liver transplantation is the treatment of choice. The aim of this study was to report the long-term outcome of patients who underwent liver transplantation for Wilson's disease. Thirteen patients with Wilson's disease, transplanted in Lyon France between January 1987 and May 2006, were including in this study: eight women and five men, aged eight to 53 years (median 20 years, seven children and six adults). The diagnosis of Wilson's disease was established before liver transplantation. The indication for liver transplantation was chronic (69%) or fulminant liver failure (31%). The median follow-up after liver transplantation was 10 years with 100% patient survival. Copper metabolism returned to normal in all patients. None of the patients with exclusive liver disease required chelation treatment after liver transplantation and none developed neurological symptoms of Wilson's disease. Liver transplantation totally reverses the abnormalities of copper metabolism and subsequent hepatic failure, but the course of neurological symptoms remains unpredictable. Long-term patient survival can be excellent without occurrence of neurological complications.
Summary Visceral leishmaniasis (VL) is a disseminated and lethal disease of reticulo-endothelial ... more Summary Visceral leishmaniasis (VL) is a disseminated and lethal disease of reticulo-endothelial system caused by protozoan parasites Leishmania donovani and L. infantum, which are known to induce host T cell suppression. To understand the impact of parasite load on T cell function, the present was focused on parasite load with T cell function in bone marrow of 26 VL patients. We observed significant enrichment of forkhead box protein 3 (FoxP3)+ (P = 0·0003) and interleukin (IL)-10+ FoxP3+ regulatory T cells (Treg) (P = 0·004) in the bone marrow (BM) of patients with high parasite load (HPL) compared with low parasite load (LPL). Concordantly, T effector cells producing interferon (IFN)-γ (P = 0·005) and IL-17A (P = 0·002) were reduced in the BM of HPL. Blocking of Treg-cell derived suppressive cytokines [(IL-10 and transforming growth factor (TGF)-β] rescued the effector T cells and their functions. However, it was observed that TGF-β levels were dominant, favouring Treg cell diffe...
Management of viral diseases relies on definite and sensitive detection methods. Citrus yellow mo... more Management of viral diseases relies on definite and sensitive detection methods. Citrus yellow mosaic virus (CYMV), a double stranded DNA virus of the genus Badnavirus, causes yellow mosaic disease in citrus plants. CYMV is transmitted through budwood and requires a robust and simplified indexing protocol for budwood certification programme. The present study reports development and standardization of an isothermal based recombinase polymerase amplification (RPA) assay for a sensitive, rapid, easy, and cost-effective method for detection and diagnosis of CYMV. Two different oligonucleotide primer sets were designed from ORF III (coding for polyprotein) and ORF II (coding for virion associated protein) regions of CYMV to perform amplification assays. Comparative evaluation of RPA, PCR and immuno-capture recombinase polymerase amplification (IC-RPA) based assays were done using purified DNA and plant crude sap. CYMV infection was efficiently detected from the crude sap in RPA and IC-R...
... Auteur(s) / Author(s). KALRA A. ; RISHI N. ; GROVER RK ; KHURANA SMP ; Affiliation(s) du ou d... more ... Auteur(s) / Author(s). KALRA A. ; RISHI N. ; GROVER RK ; KHURANA SMP ; Affiliation(s) du ou des auteurs / Author(s) Affiliation(s). Haryana agricultural univ., Hisar 125004, INDE Revue / Journal Title. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz ISSN 0340-8159 ...
Cotton leaf curl disease (CLCuD), a major threat to the cotton industry, has a complex etiology. ... more Cotton leaf curl disease (CLCuD), a major threat to the cotton industry, has a complex etiology. The prominent symptoms of CLCuD are general stunting, upward or downward curling of leaves with thickening of veins which turn dark green. Enations of ...
(English) An attempt was made to study the different factors influencing PVY-induced resistance t... more (English) An attempt was made to study the different factors influencing PVY-induced resistance to Phytophthora infestans in potato leaves. Maximum inhibition of fungal infection was observed when the interval between virus and fungus inoculation was 72 h ...
... Please recommend this service to your librarian. Title Viruses as predisposing or resistance ... more ... Please recommend this service to your librarian. Title Viruses as predisposing or resistance inducing factors in fungal pathogens. Authors Kalra, A.; Narayan Rishi Editors Prasad, D.;Puri, SN Book Crop pest and disease management: challanges for the millennium 2002 pp. ...
... RD; NARAYAN RISHI, N. Corporativo: Congress of the International Society of Sugar Cane Techno... more ... RD; NARAYAN RISHI, N. Corporativo: Congress of the International Society of Sugar Cane Technologists (14, New Orleans, 1971). Título: Occurrence of strains A and F of sugarcane mosaic virus in Uttar Pradesh (India). Proceedings. P.imprenta: Estados Unidos . Franklin. ...
There continues to be an urgent need for cost-effective prophylaxis for HPV-associated cancers in... more There continues to be an urgent need for cost-effective prophylaxis for HPV-associated cancers in socio-economically underdeveloped nations. Presently HPV vaccines, which are commercially available, are adjuvanted virus-like particles (VLPs) expressed from various recombinant expression systems. They have been characterized by different methods as safe, pure, and potent HPV vaccine antigens. We cloned and expressed L1 proteins of HPV16 & 18 in Pichia pastoris and tested their immunogenicity. We observed that HPVL1 proteins (16L1 and 18L1) are expressed in Pichia pastoris at high levels. Critical physicochemical parameters of these HPV recombinant L1 proteins were characterized by SDS PAGE, western blotting, peptide mapping, glycosylation pattern, mass spectrometry, host cell DNA and protein analysis, electron microscopy, and immunogenicity analysis. These data establish a blueprint of HPV recombinant protein antigens for standardizing & developing an alternative high-quality, cost-effective vaccine for HPV as well as similar recombinant protein-based vaccines.
Wilson's disease is a hereditary defect in hepatic copper metabolism, causing hepatic, ne... more Wilson's disease is a hereditary defect in hepatic copper metabolism, causing hepatic, neurological and/or psychiatric manifestations. For patients with severe disease, liver transplantation is the treatment of choice. The aim of this study was to report the long-term outcome of patients who underwent liver transplantation for Wilson's disease. Thirteen patients with Wilson's disease, transplanted in Lyon France between January 1987 and May 2006, were including in this study: eight women and five men, aged eight to 53 years (median 20 years, seven children and six adults). The diagnosis of Wilson's disease was established before liver transplantation. The indication for liver transplantation was chronic (69%) or fulminant liver failure (31%). The median follow-up after liver transplantation was 10 years with 100% patient survival. Copper metabolism returned to normal in all patients. None of the patients with exclusive liver disease required chelation treatment after liver transplantation and none developed neurological symptoms of Wilson's disease. Liver transplantation totally reverses the abnormalities of copper metabolism and subsequent hepatic failure, but the course of neurological symptoms remains unpredictable. Long-term patient survival can be excellent without occurrence of neurological complications.
Summary Visceral leishmaniasis (VL) is a disseminated and lethal disease of reticulo-endothelial ... more Summary Visceral leishmaniasis (VL) is a disseminated and lethal disease of reticulo-endothelial system caused by protozoan parasites Leishmania donovani and L. infantum, which are known to induce host T cell suppression. To understand the impact of parasite load on T cell function, the present was focused on parasite load with T cell function in bone marrow of 26 VL patients. We observed significant enrichment of forkhead box protein 3 (FoxP3)+ (P = 0·0003) and interleukin (IL)-10+ FoxP3+ regulatory T cells (Treg) (P = 0·004) in the bone marrow (BM) of patients with high parasite load (HPL) compared with low parasite load (LPL). Concordantly, T effector cells producing interferon (IFN)-γ (P = 0·005) and IL-17A (P = 0·002) were reduced in the BM of HPL. Blocking of Treg-cell derived suppressive cytokines [(IL-10 and transforming growth factor (TGF)-β] rescued the effector T cells and their functions. However, it was observed that TGF-β levels were dominant, favouring Treg cell diffe...
Management of viral diseases relies on definite and sensitive detection methods. Citrus yellow mo... more Management of viral diseases relies on definite and sensitive detection methods. Citrus yellow mosaic virus (CYMV), a double stranded DNA virus of the genus Badnavirus, causes yellow mosaic disease in citrus plants. CYMV is transmitted through budwood and requires a robust and simplified indexing protocol for budwood certification programme. The present study reports development and standardization of an isothermal based recombinase polymerase amplification (RPA) assay for a sensitive, rapid, easy, and cost-effective method for detection and diagnosis of CYMV. Two different oligonucleotide primer sets were designed from ORF III (coding for polyprotein) and ORF II (coding for virion associated protein) regions of CYMV to perform amplification assays. Comparative evaluation of RPA, PCR and immuno-capture recombinase polymerase amplification (IC-RPA) based assays were done using purified DNA and plant crude sap. CYMV infection was efficiently detected from the crude sap in RPA and IC-R...
... Auteur(s) / Author(s). KALRA A. ; RISHI N. ; GROVER RK ; KHURANA SMP ; Affiliation(s) du ou d... more ... Auteur(s) / Author(s). KALRA A. ; RISHI N. ; GROVER RK ; KHURANA SMP ; Affiliation(s) du ou des auteurs / Author(s) Affiliation(s). Haryana agricultural univ., Hisar 125004, INDE Revue / Journal Title. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz ISSN 0340-8159 ...
Cotton leaf curl disease (CLCuD), a major threat to the cotton industry, has a complex etiology. ... more Cotton leaf curl disease (CLCuD), a major threat to the cotton industry, has a complex etiology. The prominent symptoms of CLCuD are general stunting, upward or downward curling of leaves with thickening of veins which turn dark green. Enations of ...
(English) An attempt was made to study the different factors influencing PVY-induced resistance t... more (English) An attempt was made to study the different factors influencing PVY-induced resistance to Phytophthora infestans in potato leaves. Maximum inhibition of fungal infection was observed when the interval between virus and fungus inoculation was 72 h ...
... Please recommend this service to your librarian. Title Viruses as predisposing or resistance ... more ... Please recommend this service to your librarian. Title Viruses as predisposing or resistance inducing factors in fungal pathogens. Authors Kalra, A.; Narayan Rishi Editors Prasad, D.;Puri, SN Book Crop pest and disease management: challanges for the millennium 2002 pp. ...
... RD; NARAYAN RISHI, N. Corporativo: Congress of the International Society of Sugar Cane Techno... more ... RD; NARAYAN RISHI, N. Corporativo: Congress of the International Society of Sugar Cane Technologists (14, New Orleans, 1971). Título: Occurrence of strains A and F of sugarcane mosaic virus in Uttar Pradesh (India). Proceedings. P.imprenta: Estados Unidos . Franklin. ...
There continues to be an urgent need for cost-effective prophylaxis for HPV-associated cancers in... more There continues to be an urgent need for cost-effective prophylaxis for HPV-associated cancers in socio-economically underdeveloped nations. Presently HPV vaccines, which are commercially available, are adjuvanted virus-like particles (VLPs) expressed from various recombinant expression systems. They have been characterized by different methods as safe, pure, and potent HPV vaccine antigens. We cloned and expressed L1 proteins of HPV16 & 18 in Pichia pastoris and tested their immunogenicity. We observed that HPVL1 proteins (16L1 and 18L1) are expressed in Pichia pastoris at high levels. Critical physicochemical parameters of these HPV recombinant L1 proteins were characterized by SDS PAGE, western blotting, peptide mapping, glycosylation pattern, mass spectrometry, host cell DNA and protein analysis, electron microscopy, and immunogenicity analysis. These data establish a blueprint of HPV recombinant protein antigens for standardizing & developing an alternative high-quality, cost-effective vaccine for HPV as well as similar recombinant protein-based vaccines.
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