Ceftriaxone sodium is a third-generation cephalosporin antibiotic. Like other third-generation ce... more Ceftriaxone sodium is a third-generation cephalosporin antibiotic. Like other third-generation cephalosporins, it has broad spectrum activity against Gram positive and Gram-negative bacteria. In most cases, it is considered to be equivalent to cefotaximein in terms of safety and efficacy. In the present study an HPLC method was developed and validated for the assay of ceftriaxone sodium injection. The chromatographic system was equipped with Xterra C18 (4.6 x 150mm, 5 m, Make:Waters) column and detector set at 242 nm, in conjunction with a mobile phase of Disodium hydrogen phosphate buffer and Acetonitrile in the ratio of 65:35 (pH 4.3, adjusted with orthophosphoric acid) at a flow rate of 1.0ml/min and the injection volume set at 20 μl with 9 minutes of runtime. The described method was linear over a concentration range of 10.0 -100.0 μg/ml for the assay of ceftriaxone sodium with a with good linearity response of 0.999. The retention time of ceftriaxone sodium was 2.6 min ± 0.02. ...
The present work is a method development and validation for the determination of Ledipasvir by us... more The present work is a method development and validation for the determination of Ledipasvir by using reverse phase High performance liquid chromatography (HPLC). The procedure involved use of isocratic elution where the stationary phase was a BDS column (250 mm, 4.6 mm, 5 µm), mobile phase 0.05% triflouroacetic acid in methanol and 0.05% triflouroacetic acid in acetonitrile (55:45). pH of the chromatographic system was maintained at 3.0, flow rate 1 ml/minute, eluent was monitored by PDA detector wavelength at 270 nm. Retention time was found to be 2.749 minutes, regression analysis shown the value of correlation coefficient 0.999. Value for limit of detection (LOD) was 1.064µgm/ml and limit of quantification (LOQ) was 3.224 µgm/ml. Linearity range was designed 15µg/ml to 300µg/ml for Ledipasvir. Accuracy study revealed percentage recovery 99.81%-100.10% and precision result in terms of standard deviation and percentage relative standard deviation was 1986.515 and 0.08511%. Test for intermediate results also were well within the limit for Ledipasvir. Robustness study results proved the suitability of the method under different chromatographic condition. The developed method was validated as per ICH guideline and was found to be an ideal one for regular analysis in the laboratory.
Ceftriaxone sodium is a third-generation cephalosporin antibiotic. Like other third-generation ce... more Ceftriaxone sodium is a third-generation cephalosporin antibiotic. Like other third-generation cephalosporins, it has broad spectrum activity against Gram positive and Gram-negative bacteria. In most cases, it is considered to be equivalent to cefotaximein in terms of safety and efficacy. In the present study an HPLC method was developed and validated for the assay of ceftriaxone sodium injection. The chromatographic system was equipped with Xterra C18 (4.6 x 150mm, 5 m, Make:Waters) column and detector set at 242 nm, in conjunction with a mobile phase of Disodium hydrogen phosphate buffer and Acetonitrile in the ratio of 65:35 (pH 4.3, adjusted with orthophosphoric acid) at a flow rate of 1.0ml/min and the injection volume set at 20 μl with 9 minutes of runtime. The described method was linear over a concentration range of 10.0 -100.0 μg/ml for the assay of ceftriaxone sodium with a with good linearity response of 0.999. The retention time of ceftriaxone sodium was 2.6 min ± 0.02. ...
The present work is a method development and validation for the determination of Ledipasvir by us... more The present work is a method development and validation for the determination of Ledipasvir by using reverse phase High performance liquid chromatography (HPLC). The procedure involved use of isocratic elution where the stationary phase was a BDS column (250 mm, 4.6 mm, 5 µm), mobile phase 0.05% triflouroacetic acid in methanol and 0.05% triflouroacetic acid in acetonitrile (55:45). pH of the chromatographic system was maintained at 3.0, flow rate 1 ml/minute, eluent was monitored by PDA detector wavelength at 270 nm. Retention time was found to be 2.749 minutes, regression analysis shown the value of correlation coefficient 0.999. Value for limit of detection (LOD) was 1.064µgm/ml and limit of quantification (LOQ) was 3.224 µgm/ml. Linearity range was designed 15µg/ml to 300µg/ml for Ledipasvir. Accuracy study revealed percentage recovery 99.81%-100.10% and precision result in terms of standard deviation and percentage relative standard deviation was 1986.515 and 0.08511%. Test for intermediate results also were well within the limit for Ledipasvir. Robustness study results proved the suitability of the method under different chromatographic condition. The developed method was validated as per ICH guideline and was found to be an ideal one for regular analysis in the laboratory.
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