Archives of Pediatrics & Adolescent Medicine, 1985
We studied a group of 22 children with an acute upper intestinal obstruction associated with segm... more We studied a group of 22 children with an acute upper intestinal obstruction associated with segmental necrotizing enteritis, usually involving the jejunum. No similar cases were found in the literature.
A method for in vitro regeneration of Maerua oblongifolia (Capparaceae) from nodal shoot explants... more A method for in vitro regeneration of Maerua oblongifolia (Capparaceae) from nodal shoot explants is outlined. Percent shoot response with multiplication rate (21.1± 2.33) shoots per explant (30 mm length) was achieved when cultured on semisolid Murashige and Skoog (MS) medium containing 3% sucrose and supplemented with 2.0 mgl-1 of (Benzylaminopurine) BAP + additives (25.0 mgl-1adenine sulphate + 25.0 mgl-1 citric acid + 50.0 mgl-1 ascorbic acid). Further amplification of shoots was achieved when concentration of BAP was lowered (0.25 mgl -1) and Kinetin (0.25 mgl -1) along with 0.1 mgl -1 IAA was incorporated in the MS medium. A maximum of 58.1 ± 3.88 shoots of length 4-5 cm were obtained. The in vitro regenerated shoots rooted in vitro on half-strength MS medium containing 3.0 mgl-1 of IBA. About 85% of shoot rooted (4.04 ± 0.96 roots per shoot) on this medium. Other auxins such as NOA also promoted rooting but, the response in terms of percentage of rooting (75%) and shoot numbe...
Rauvolfia serpentina Benth. ex Kurz called sarpagandha(the snake root) in Hindi, belongs to famil... more Rauvolfia serpentina Benth. ex Kurz called sarpagandha(the snake root) in Hindi, belongs to family Apocynaceae. This plant is listed in earliest Ayurvedic medicinal text the Charaka Samhita(c. 700 B.C.) and has been used since at least that time to treat mental illness and insomnia. The roots of R. serpentina contain numerous alkaloids. Its indiscriminate use and poor method of conventional
Rauvolfia serpentina Benth. ex Kurz called sarpagandha(the snake root) in Hindi, belongs to famil... more Rauvolfia serpentina Benth. ex Kurz called sarpagandha(the snake root) in Hindi, belongs to family Apocynaceae. This plant is listed in earliest Ayurvedic medicinal text the Charaka Samhita(c. 700 B.C.) and has been used since at least that time to treat mental illness and insomnia. The roots of R. serpentina contain numerous alkaloids. Its indiscriminate use and poor method of conventional propagation have led this species to be included in the list of endangered plants. Thus, a need arises to generate an efficient protocol for cloning of R. serpentina in order to regenerate propagules to replenish depleting forests and meeting the demand of commercial cultivation. We report here on development of an efficient and simple process for large-scale clonal micropropagation of selected plants of R. serpentina and their successful field establishment. Nodal shoot explants produced multiple shoots (3-5 shoots per node) by axillary bud proliferation on Murashige and Skoog (MS) medium + 10 μ...
ABSTRACT Prosopis cineraria (L.) Druce, family Fabaceae, is an important multipurpose tree specie... more ABSTRACT Prosopis cineraria (L.) Druce, family Fabaceae, is an important multipurpose tree species of the Indian Thar Desert. It is an extremely drought and heat tolerant plant and has ability to survive in highly alkaline and saline environments. Considering the commercial, horticultural and ecological importance of P. cineraria, there is a great need to develop genomic resources in the form of microsatellite markers for the genetic enhancement of this crop. We identified and characterized 10 microsatellite markers in P. cineraria by cross species amplification. Total 18 Simple Sequence Repeat (SSR) primer pairs developed in P. chilensis, P. alba, and P. flexuosa were used to amplify SSR loci in P. cineraria. Out of eighteen SSR markers tested, ten (55.5%) amplified recognizable amplicons. The number of alleles detected at each locus ranged from one to four, a total of 24 with an average of 2.4 alleles. Observed heterozygosity (Ho) and expected heterozygosity (He) values varied from 0.14 to 0.85 and 0.21 to 0.56 with an average of 0.47 and 0.37, respectively. The polymorphic information content (PIC) values ranged from 0.49 to 0.78 with an average of 0.66. Of the nine polymorphic markers, seven were highly informative and polymorphic (PIC > 0.5). These microsatellite markers are characterized for the first time in P. cineraria. All microsatellite markers identified in this study may be useful in comparative genomics and population genetics studies of P. cineraria.
Momordica dioica Roxb. ex Willd., is a perennial and dioecious (2n = 28) plant of family Cucurbit... more Momordica dioica Roxb. ex Willd., is a perennial and dioecious (2n = 28) plant of family Cucurbitaceae. Conventional methods of propagation through seeds, stem cuttings and rhizomatous/tuberous roots are inadequate for its mass cultivation as a vegetable crop. This paper reports an improved and efficient micropropagation method for wild female M. dioica using nodal explants. Shoot amplification was achieved using subculturing of in vitro raised shoots on MS medium supplemented with various concentrations of 6-benzylaminopurine (BAP) alone or in combination with indole-3-acetic acid (IAA). The maximum number of shoots (45.30 ± 3.83) with an average length 6.52 ± 0.89 cm were differentiated on MS medium containing 0.5 mg L-1 BAP, 0.1 mg L-1 IAA and additives (50 mg L-1 ascorbic acid, 25 mg L-1 each of adenine sulphate, citric acid and l-arginine). The cloned shoots were rooted ex vitro. Each shoot treated with 250 mg L-1 IBA for 5 min produced 12.3 ± 1.33 with a mean length 5.4 ± 0.73 cm. More than 85% (46 plants) of ex vitro rooted plantlets were successfully hardened in a greenhouse with normal growth characteristics. In order to evaluate the genetic stability of micropropagated plants, the two PCR-based techniques, Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) were used. The amplification patterns of the micropropagated and mother plant were monomorphic thus depicting genetic stability of the micropropagation system. This protocol could be effectively employed for the mass multiplication of wild female M. dioica, a popular summer vegetable crop.
Present study demonstrated the cross-genera transferability of 23 simple sequence repeat (SSR) pr... more Present study demonstrated the cross-genera transferability of 23 simple sequence repeat (SSR) primer pairs developed for guava (Psidium guajava L.) to four new targets, two species of eucalypts (Eucalyptus citriodora, Eucalyptus camaldulensis), bottlebrush (Callistemon lanceolatus) and clove (Syzygium aromaticum), belonging to the family Myrtaceae and subfamily Myrtoideae. Off the 23 SSR loci assayed, 18 (78.2%) gave cross-amplification in E. citriodora, 14 (60.8%) in E. camaldulensis and 17-17 (73.9%) in C. lanceolatus and S. aromaticum. Eight primer pairs were found to be transferable to all four species. The number of alleles detected at each locus ranged from one to nine, with an average of 4.8, 2.6, 4.5 and 4.6 alleles in E. citriodora, E. camaldulensis, C. lanceolatus and S. aromaticum, respectively. The high levels of cross-genera transferability of guava SSRs may be applicable for the analysis of intra- and inter specific genetic diversity of target species, especially in E. citriodora, C. lanceolatus and S. aromaticum, for which till date no information about EST-derived as well as genomic SSR is available.
The present study described an improved and reproducible in vitro regeneration system for Termina... more The present study described an improved and reproducible in vitro regeneration system for Terminalia arjuna using nodal segment explants obtained from a mature plant. Shoot tips excised from in vitro proliferated shoots were encapsulated in 3 % sodium alginate and 100 mM CaCl2[Symbol: see text]2H2O for the development of synthetic seeds which may be applicable in short-term storage and germplasm exchange of elite genotype. Shoot multiplication was significantly influenced by a number of factors, namely types and concentrations of plant growth regulators, medium composition, repeated transfer of mother explants, subculturing of in vitro regenerated shoot clumps, agar concentrations, and temperature. Maximum numbers of shoots (16.50 ± 3.67) were observed on modified Murashige and Skoog (MMS) medium containing 0.5 mg l(-1) of benzylaminopurine (BAP) and 0.1 mg l(-1) of naphthalene acetic acid (NAA). To shortening the regeneration pathway, rooting of micropropagated shoots under in vitro condition was excluded and an experiment on ex vitro rooting was conducted and it was observed that the highest percentage of shoots rooted ex vitro when treated with indole-3-butyric acid (IBA, 250 mg l(-1)) + 2-naphthoxy acetic acid (NOA, 250 mg l(-1)) for 5 min. The well-developed ex vitro rooted shoots were acclimatized successfully in soilrite under greenhouse conditions with 80 % survival of plants. Randomly amplified polymorphic DNA (RAPD) analysis confirmed that all the regenerated plants were genetically identical to the mother plant, suggesting the absence of detectable genetic variation in the regenerated plantlets. To the best of our knowledge, this is the first report on synthetic seed production as well as ex vitro rooting and genetic fidelity assessment of micropropagated shoots of T. arjuna.
Archives of Pediatrics & Adolescent Medicine, 1985
We studied a group of 22 children with an acute upper intestinal obstruction associated with segm... more We studied a group of 22 children with an acute upper intestinal obstruction associated with segmental necrotizing enteritis, usually involving the jejunum. No similar cases were found in the literature.
A method for in vitro regeneration of Maerua oblongifolia (Capparaceae) from nodal shoot explants... more A method for in vitro regeneration of Maerua oblongifolia (Capparaceae) from nodal shoot explants is outlined. Percent shoot response with multiplication rate (21.1± 2.33) shoots per explant (30 mm length) was achieved when cultured on semisolid Murashige and Skoog (MS) medium containing 3% sucrose and supplemented with 2.0 mgl-1 of (Benzylaminopurine) BAP + additives (25.0 mgl-1adenine sulphate + 25.0 mgl-1 citric acid + 50.0 mgl-1 ascorbic acid). Further amplification of shoots was achieved when concentration of BAP was lowered (0.25 mgl -1) and Kinetin (0.25 mgl -1) along with 0.1 mgl -1 IAA was incorporated in the MS medium. A maximum of 58.1 ± 3.88 shoots of length 4-5 cm were obtained. The in vitro regenerated shoots rooted in vitro on half-strength MS medium containing 3.0 mgl-1 of IBA. About 85% of shoot rooted (4.04 ± 0.96 roots per shoot) on this medium. Other auxins such as NOA also promoted rooting but, the response in terms of percentage of rooting (75%) and shoot numbe...
Rauvolfia serpentina Benth. ex Kurz called sarpagandha(the snake root) in Hindi, belongs to famil... more Rauvolfia serpentina Benth. ex Kurz called sarpagandha(the snake root) in Hindi, belongs to family Apocynaceae. This plant is listed in earliest Ayurvedic medicinal text the Charaka Samhita(c. 700 B.C.) and has been used since at least that time to treat mental illness and insomnia. The roots of R. serpentina contain numerous alkaloids. Its indiscriminate use and poor method of conventional
Rauvolfia serpentina Benth. ex Kurz called sarpagandha(the snake root) in Hindi, belongs to famil... more Rauvolfia serpentina Benth. ex Kurz called sarpagandha(the snake root) in Hindi, belongs to family Apocynaceae. This plant is listed in earliest Ayurvedic medicinal text the Charaka Samhita(c. 700 B.C.) and has been used since at least that time to treat mental illness and insomnia. The roots of R. serpentina contain numerous alkaloids. Its indiscriminate use and poor method of conventional propagation have led this species to be included in the list of endangered plants. Thus, a need arises to generate an efficient protocol for cloning of R. serpentina in order to regenerate propagules to replenish depleting forests and meeting the demand of commercial cultivation. We report here on development of an efficient and simple process for large-scale clonal micropropagation of selected plants of R. serpentina and their successful field establishment. Nodal shoot explants produced multiple shoots (3-5 shoots per node) by axillary bud proliferation on Murashige and Skoog (MS) medium + 10 μ...
ABSTRACT Prosopis cineraria (L.) Druce, family Fabaceae, is an important multipurpose tree specie... more ABSTRACT Prosopis cineraria (L.) Druce, family Fabaceae, is an important multipurpose tree species of the Indian Thar Desert. It is an extremely drought and heat tolerant plant and has ability to survive in highly alkaline and saline environments. Considering the commercial, horticultural and ecological importance of P. cineraria, there is a great need to develop genomic resources in the form of microsatellite markers for the genetic enhancement of this crop. We identified and characterized 10 microsatellite markers in P. cineraria by cross species amplification. Total 18 Simple Sequence Repeat (SSR) primer pairs developed in P. chilensis, P. alba, and P. flexuosa were used to amplify SSR loci in P. cineraria. Out of eighteen SSR markers tested, ten (55.5%) amplified recognizable amplicons. The number of alleles detected at each locus ranged from one to four, a total of 24 with an average of 2.4 alleles. Observed heterozygosity (Ho) and expected heterozygosity (He) values varied from 0.14 to 0.85 and 0.21 to 0.56 with an average of 0.47 and 0.37, respectively. The polymorphic information content (PIC) values ranged from 0.49 to 0.78 with an average of 0.66. Of the nine polymorphic markers, seven were highly informative and polymorphic (PIC > 0.5). These microsatellite markers are characterized for the first time in P. cineraria. All microsatellite markers identified in this study may be useful in comparative genomics and population genetics studies of P. cineraria.
Momordica dioica Roxb. ex Willd., is a perennial and dioecious (2n = 28) plant of family Cucurbit... more Momordica dioica Roxb. ex Willd., is a perennial and dioecious (2n = 28) plant of family Cucurbitaceae. Conventional methods of propagation through seeds, stem cuttings and rhizomatous/tuberous roots are inadequate for its mass cultivation as a vegetable crop. This paper reports an improved and efficient micropropagation method for wild female M. dioica using nodal explants. Shoot amplification was achieved using subculturing of in vitro raised shoots on MS medium supplemented with various concentrations of 6-benzylaminopurine (BAP) alone or in combination with indole-3-acetic acid (IAA). The maximum number of shoots (45.30 ± 3.83) with an average length 6.52 ± 0.89 cm were differentiated on MS medium containing 0.5 mg L-1 BAP, 0.1 mg L-1 IAA and additives (50 mg L-1 ascorbic acid, 25 mg L-1 each of adenine sulphate, citric acid and l-arginine). The cloned shoots were rooted ex vitro. Each shoot treated with 250 mg L-1 IBA for 5 min produced 12.3 ± 1.33 with a mean length 5.4 ± 0.73 cm. More than 85% (46 plants) of ex vitro rooted plantlets were successfully hardened in a greenhouse with normal growth characteristics. In order to evaluate the genetic stability of micropropagated plants, the two PCR-based techniques, Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) were used. The amplification patterns of the micropropagated and mother plant were monomorphic thus depicting genetic stability of the micropropagation system. This protocol could be effectively employed for the mass multiplication of wild female M. dioica, a popular summer vegetable crop.
Present study demonstrated the cross-genera transferability of 23 simple sequence repeat (SSR) pr... more Present study demonstrated the cross-genera transferability of 23 simple sequence repeat (SSR) primer pairs developed for guava (Psidium guajava L.) to four new targets, two species of eucalypts (Eucalyptus citriodora, Eucalyptus camaldulensis), bottlebrush (Callistemon lanceolatus) and clove (Syzygium aromaticum), belonging to the family Myrtaceae and subfamily Myrtoideae. Off the 23 SSR loci assayed, 18 (78.2%) gave cross-amplification in E. citriodora, 14 (60.8%) in E. camaldulensis and 17-17 (73.9%) in C. lanceolatus and S. aromaticum. Eight primer pairs were found to be transferable to all four species. The number of alleles detected at each locus ranged from one to nine, with an average of 4.8, 2.6, 4.5 and 4.6 alleles in E. citriodora, E. camaldulensis, C. lanceolatus and S. aromaticum, respectively. The high levels of cross-genera transferability of guava SSRs may be applicable for the analysis of intra- and inter specific genetic diversity of target species, especially in E. citriodora, C. lanceolatus and S. aromaticum, for which till date no information about EST-derived as well as genomic SSR is available.
The present study described an improved and reproducible in vitro regeneration system for Termina... more The present study described an improved and reproducible in vitro regeneration system for Terminalia arjuna using nodal segment explants obtained from a mature plant. Shoot tips excised from in vitro proliferated shoots were encapsulated in 3 % sodium alginate and 100 mM CaCl2[Symbol: see text]2H2O for the development of synthetic seeds which may be applicable in short-term storage and germplasm exchange of elite genotype. Shoot multiplication was significantly influenced by a number of factors, namely types and concentrations of plant growth regulators, medium composition, repeated transfer of mother explants, subculturing of in vitro regenerated shoot clumps, agar concentrations, and temperature. Maximum numbers of shoots (16.50 ± 3.67) were observed on modified Murashige and Skoog (MMS) medium containing 0.5 mg l(-1) of benzylaminopurine (BAP) and 0.1 mg l(-1) of naphthalene acetic acid (NAA). To shortening the regeneration pathway, rooting of micropropagated shoots under in vitro condition was excluded and an experiment on ex vitro rooting was conducted and it was observed that the highest percentage of shoots rooted ex vitro when treated with indole-3-butyric acid (IBA, 250 mg l(-1)) + 2-naphthoxy acetic acid (NOA, 250 mg l(-1)) for 5 min. The well-developed ex vitro rooted shoots were acclimatized successfully in soilrite under greenhouse conditions with 80 % survival of plants. Randomly amplified polymorphic DNA (RAPD) analysis confirmed that all the regenerated plants were genetically identical to the mother plant, suggesting the absence of detectable genetic variation in the regenerated plantlets. To the best of our knowledge, this is the first report on synthetic seed production as well as ex vitro rooting and genetic fidelity assessment of micropropagated shoots of T. arjuna.
Uploads