The influence of a high-lipid and high-carbohydrate diet, as inducers of the development of metab... more The influence of a high-lipid and high-carbohydrate diet, as inducers of the development of metabolic syndrome on the prooxidant-antioxidant state of rat erythrocytes have been studied. Oxidative damage of cellular components was evaluated by the content of thiobarbituric acid reactive substances (TBARS) as well as products of oxidative modification of proteins of a neutral and basic nature. The antioxidant potential of erythrocytes was determined by the activity of superoxide dismutase, catalase, and glutathione peroxidase. Manipulations with diets, especially a high-lipid diet in which an additional source of lipids was olive oil and high-carbohydrate diet, involving the consumption of 10% fructose solution by animals, promoted the development of oxidative stress in rats' erythrocytes. It has been shown that a 28-day high-lipid diet was not accompanied by significant variation in TBARS and oxidized protein modification products but was accompanied by significantly reduced catalase and glutathione peroxidase activity in erythrocyte hemolysate. The longer duration of exposure to the high-lipid diet (42 days) was accompanied by an increase in the content of TBARS against the background of reduced superoxide dismutase and catalase activity. When using fructose for 28 days for the induction of metabolic syndrome, no significant changes in the content of products of oxidative modification of lipids and protein were shown. Instead, a longer effect of a high-carbohydrate diet (42 days) is accompanied by an increase in the content of TBARS. The use of fructose for 28 days caused a decrease in the activity of superoxide dismutase, catalase, and glutathione peroxidase. A similar tendency to decreased activity of the studied enzymes was demonstrated when animals consumed fructose for 42 days. The obtained results allowed us to conclude that the assessment of redox balance of erythrocytes in metabolic syndrome is a rational and convenient method of studying the prooxidant-antioxidant state of the body.
Introduction. Metabolic syndrome is a cluster of metabolic abnormalities that includes hypertensi... more Introduction. Metabolic syndrome is a cluster of metabolic abnormalities that includes hypertension, central obesity, insulin resistance and atherogenic dyslipidemia. Given the wide geographical distribution and growing number of people suffering from this disease, there is an urgent need in developing animal models that would accurately reproduce the development of all symptoms of human metabolic syndrome (insulin resistance, dyslipidemia, obesity and hypertension). The most cost-effective method related to the real causes of metabolic syndrome is the use of different types of diets. Materials and Methods. The study was performed on white outbred male rats about 6 months old and weighing 300–400 g. The metabolic syndrome was induced by high-fat and high-carbohydrate diets. The lipid-enriched diet involved the consumption of regular chow diet for laboratory animals with additional fat content (40 % by weight of chow). The source of additional lipids was olive oil, which is rich in m...
Wstep. Scharakteryzowanie aktywności granulocytow obojetnochlonnych wielojądrzastych w cukrzycy m... more Wstep. Scharakteryzowanie aktywności granulocytow obojetnochlonnych wielojądrzastych w cukrzycy ma zasadnicze znaczenie dla zrozumienia procesow immunologicznych uczestniczących w patogenezie tej choroby. Poszukiwanie skutecznych lekow, ktore moglyby wyciszyc i/lub zmienic odpowiedź immunologiczną, najlepiej bez niekorzystnych efektow, jest obiecującym obszarem wspolczesnych badan. Jako obiekt badan nad mozliwościami korygowania dysfunkcji ukladu immunologicznego wybrano Smallanthus sonchifolius (Poepp. et Endl), czyli yacon — rośline od dawna stosowaną w medycynie ludowej do leczenia cukrzycy. Badanie wykonano w celu poszukiwania skutecznego leku regulującego czynnośc neutrofili w warunkach wywolanej doświadczalnie cukrzycy typu 1. Material i metody. Eksperyment przeprowadzono na szczurach Wistar plci meskiej. Cukrzyce wywolano przez dootrzewnową iniekcje streptozotocyny. W celu oceny aktywności fagocytarnej oraz zawartości bialek kationowych i mieloperoksydazy w neutrofilach wykon...
We obtained red wine concentrate, which was enriched with natural polyphenolic compounds (PC conc... more We obtained red wine concentrate, which was enriched with natural polyphenolic compounds (PC concentrate). The main purpose was to study the hypoglycemic and antioxidant effects of the red wine concentrate, and its impact on key hematological parameters of rats with experimental diabetes mellitus. While administrating the red wine concentrate to rats with diabetes, partial recovering of glucose tolerance was promoted, as well as normalization of glycated hemoglobin level, an increase in the quantity of erythrocytes and hemoglobin concentration. PC concentrate had anti-radical effect, which was determined using 2,2-diphenyl-1-picrylhydrazylradical (DPPH) method and effectively inhibited oxidation of phosphatidylcholine liposomes, induced by 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH) as a free radical generator. It was also confirmed that PC concentrate had antioxidant properties in vivo. The contents of lipid peroxidation and protein oxidation products, the activity of cata...
Hypoglycemic and antioxidant properties of extracts of medicinal plants Galega officinalis L. (ab... more Hypoglycemic and antioxidant properties of extracts of medicinal plants Galega officinalis L. (aboveground part) and yacon (Smallanthus sonchifolius Poepp. & Endl.) (leaves) as potential sources of biologically active substances with antidiabetic action have been studied. The pronounced hypoglycemic effect of Galega officinalis extract, devoid of alkaloids, at a dose of 600 mg/kg in experimental diabetes mellitus (DM) has been proven. The established effect is evidenced by a decrease in the concentration of glucose and glycosylated hemoglobin in the blood, increase glucose tolerance of cells, increase C-peptide and insulin content in the plasma of rats’ blood. The effective hypoglycemic effect of the extract in the studied pathology was confirmed by histological examination of the pancreas. The cytoprotective effect of the studied extract on pancreatic cells at a dose of 1200 mg/kg was experimentally confirmed. In the standard cut area, an increase was found in the number of Langerh...
The influence of a high-lipid and high-carbohydrate diet, as inducers of the development of metab... more The influence of a high-lipid and high-carbohydrate diet, as inducers of the development of metabolic syndrome on the prooxidant-antioxidant state of rat erythrocytes have been studied. Oxidative damage of cellular components was evaluated by the content of thiobarbituric acid reactive substances (TBARS) as well as products of oxidative modification of proteins of a neutral and basic nature. The antioxidant potential of erythrocytes was determined by the activity of superoxide dismutase, catalase, and glutathione peroxidase. Manipulations with diets, especially a high-lipid diet in which an additional source of lipids was olive oil and high-carbohydrate diet, involving the consumption of 10% fructose solution by animals, promoted the development of oxidative stress in rats' erythrocytes. It has been shown that a 28-day high-lipid diet was not accompanied by significant variation in TBARS and oxidized protein modification products but was accompanied by significantly reduced catalase and glutathione peroxidase activity in erythrocyte hemolysate. The longer duration of exposure to the high-lipid diet (42 days) was accompanied by an increase in the content of TBARS against the background of reduced superoxide dismutase and catalase activity. When using fructose for 28 days for the induction of metabolic syndrome, no significant changes in the content of products of oxidative modification of lipids and protein were shown. Instead, a longer effect of a high-carbohydrate diet (42 days) is accompanied by an increase in the content of TBARS. The use of fructose for 28 days caused a decrease in the activity of superoxide dismutase, catalase, and glutathione peroxidase. A similar tendency to decreased activity of the studied enzymes was demonstrated when animals consumed fructose for 42 days. The obtained results allowed us to conclude that the assessment of redox balance of erythrocytes in metabolic syndrome is a rational and convenient method of studying the prooxidant-antioxidant state of the body.
Introduction. Metabolic syndrome is a cluster of metabolic abnormalities that includes hypertensi... more Introduction. Metabolic syndrome is a cluster of metabolic abnormalities that includes hypertension, central obesity, insulin resistance and atherogenic dyslipidemia. Given the wide geographical distribution and growing number of people suffering from this disease, there is an urgent need in developing animal models that would accurately reproduce the development of all symptoms of human metabolic syndrome (insulin resistance, dyslipidemia, obesity and hypertension). The most cost-effective method related to the real causes of metabolic syndrome is the use of different types of diets. Materials and Methods. The study was performed on white outbred male rats about 6 months old and weighing 300–400 g. The metabolic syndrome was induced by high-fat and high-carbohydrate diets. The lipid-enriched diet involved the consumption of regular chow diet for laboratory animals with additional fat content (40 % by weight of chow). The source of additional lipids was olive oil, which is rich in m...
Wstep. Scharakteryzowanie aktywności granulocytow obojetnochlonnych wielojądrzastych w cukrzycy m... more Wstep. Scharakteryzowanie aktywności granulocytow obojetnochlonnych wielojądrzastych w cukrzycy ma zasadnicze znaczenie dla zrozumienia procesow immunologicznych uczestniczących w patogenezie tej choroby. Poszukiwanie skutecznych lekow, ktore moglyby wyciszyc i/lub zmienic odpowiedź immunologiczną, najlepiej bez niekorzystnych efektow, jest obiecującym obszarem wspolczesnych badan. Jako obiekt badan nad mozliwościami korygowania dysfunkcji ukladu immunologicznego wybrano Smallanthus sonchifolius (Poepp. et Endl), czyli yacon — rośline od dawna stosowaną w medycynie ludowej do leczenia cukrzycy. Badanie wykonano w celu poszukiwania skutecznego leku regulującego czynnośc neutrofili w warunkach wywolanej doświadczalnie cukrzycy typu 1. Material i metody. Eksperyment przeprowadzono na szczurach Wistar plci meskiej. Cukrzyce wywolano przez dootrzewnową iniekcje streptozotocyny. W celu oceny aktywności fagocytarnej oraz zawartości bialek kationowych i mieloperoksydazy w neutrofilach wykon...
We obtained red wine concentrate, which was enriched with natural polyphenolic compounds (PC conc... more We obtained red wine concentrate, which was enriched with natural polyphenolic compounds (PC concentrate). The main purpose was to study the hypoglycemic and antioxidant effects of the red wine concentrate, and its impact on key hematological parameters of rats with experimental diabetes mellitus. While administrating the red wine concentrate to rats with diabetes, partial recovering of glucose tolerance was promoted, as well as normalization of glycated hemoglobin level, an increase in the quantity of erythrocytes and hemoglobin concentration. PC concentrate had anti-radical effect, which was determined using 2,2-diphenyl-1-picrylhydrazylradical (DPPH) method and effectively inhibited oxidation of phosphatidylcholine liposomes, induced by 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH) as a free radical generator. It was also confirmed that PC concentrate had antioxidant properties in vivo. The contents of lipid peroxidation and protein oxidation products, the activity of cata...
Hypoglycemic and antioxidant properties of extracts of medicinal plants Galega officinalis L. (ab... more Hypoglycemic and antioxidant properties of extracts of medicinal plants Galega officinalis L. (aboveground part) and yacon (Smallanthus sonchifolius Poepp. & Endl.) (leaves) as potential sources of biologically active substances with antidiabetic action have been studied. The pronounced hypoglycemic effect of Galega officinalis extract, devoid of alkaloids, at a dose of 600 mg/kg in experimental diabetes mellitus (DM) has been proven. The established effect is evidenced by a decrease in the concentration of glucose and glycosylated hemoglobin in the blood, increase glucose tolerance of cells, increase C-peptide and insulin content in the plasma of rats’ blood. The effective hypoglycemic effect of the extract in the studied pathology was confirmed by histological examination of the pancreas. The cytoprotective effect of the studied extract on pancreatic cells at a dose of 1200 mg/kg was experimentally confirmed. In the standard cut area, an increase was found in the number of Langerh...
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