Naïve Felidae in the wild may harbor infectious viruses of importance due to cross-species transm... more Naïve Felidae in the wild may harbor infectious viruses of importance due to cross-species transmission between the domesticated animals or human–wildlife contact. However, limited information is available on virus shedding or viremia in the captive wild felids, especially in Malaysia. Four infectious viruses of cat, feline herpesvirus (FHV), feline calicivirus (FCV), canine distemper virus (CDV), and canine parvovirus (CPV), were screened in leopards, feral cats, and tigers in Malaysia based on virus isolation in Crandell-Rees feline kidney (CRFK) cells, PCR/RT-PCR, and whole-genome sequencing analysis of the positive isolate. From a total of 36 sera collected, 11 samples showed three consecutive cytopathic effects in the cell culture and were subjected to PCR using specific primers for FHV, FCV, CDV, and CPV. Only one sample from a Malayan tiger was detected positive for CPV. The entire viral genome of CPV (UPM-CPV15/P. tigris jacksoni; GenBank Accession number MW380384) was ampli...
The complete genome sequence of the ALL-03 strain of rat cytomegalovirus (RCMV) has been determin... more The complete genome sequence of the ALL-03 strain of rat cytomegalovirus (RCMV) has been determined. The RCMV genome has a length of 197,958 bp and is arranged as a single unique sequence flanked by 504-bp terminal direct repeats. This strain is closely related to the English strain of RCMV in terms of genetic arrangement but differs slightly in size.
This study probes into the prospect of cross-reactivity of HCMV with RCMV which has not been ackn... more This study probes into the prospect of cross-reactivity of HCMV with RCMV which has not been acknowledged to date. We describe the uncovering of a protein with an estimated size of between 61-68 kDa from local RCMV strains which reacted with HCMV positive sera. Our findings are a first disclosure of a plausible immunological cross-reactivity between RCMV with its human counterpart which grounds substantial interest implying existence of conserved determinants between rat and human CMV polypeptides. The cross-reactive protein most likely represents an enveloped glycoprotein, though the precise identification and its degree of similarity needs to be evidently defined and further elucidated in forthcoming experiments.
Xenotransplantation has generated considerable interest as a potential solution to provide an ind... more Xenotransplantation has generated considerable interest as a potential solution to provide an indefinite source of islet cells for transplantation into diabetic recipients. the present study investigates the prospective use of caprine islet cellsas an alternative source of pancreatic islets for xenotransplantation. the objectives of the study were to evaluate transplanted caprine islets viability and their functionality following transplantation into diabetic mice. in this study,caprine pancreases were collected from a local slaughterhouse and transported to the laboratory by maintaining the cold chain. islets were obtained by a collagenase-based digestion. islet cell purity and viability were evaluated by dithizone and pi staining, respectively. following static incubation, an in vitro insulin secretion assay was carried out and analyzed by elisa. equal volumes of caprine islets (2500 islets equivalent) were transplanted under the kidney capsules of streptozotocin induced diabetic ...
Naïve Felidae in the wild may harbor infectious viruses of importance due to cross-species transm... more Naïve Felidae in the wild may harbor infectious viruses of importance due to cross-species transmission between the domesticated animals or human–wildlife contact. However, limited information is available on virus shedding or viremia in the captive wild felids, especially in Malaysia. Four infectious viruses of cat, feline herpesvirus (FHV), feline calicivirus (FCV), canine distemper virus (CDV), and canine parvovirus (CPV), were screened in leopards, feral cats, and tigers in Malaysia based on virus isolation in Crandell-Rees feline kidney (CRFK) cells, PCR/RT-PCR, and whole-genome sequencing analysis of the positive isolate. From a total of 36 sera collected, 11 samples showed three consecutive cytopathic effects in the cell culture and were subjected to PCR using specific primers for FHV, FCV, CDV, and CPV. Only one sample from a Malayan tiger was detected positive for CPV. The entire viral genome of CPV (UPM-CPV15/P. tigris jacksoni; GenBank Accession number MW380384) was ampli...
The complete genome sequence of the ALL-03 strain of rat cytomegalovirus (RCMV) has been determin... more The complete genome sequence of the ALL-03 strain of rat cytomegalovirus (RCMV) has been determined. The RCMV genome has a length of 197,958 bp and is arranged as a single unique sequence flanked by 504-bp terminal direct repeats. This strain is closely related to the English strain of RCMV in terms of genetic arrangement but differs slightly in size.
This study probes into the prospect of cross-reactivity of HCMV with RCMV which has not been ackn... more This study probes into the prospect of cross-reactivity of HCMV with RCMV which has not been acknowledged to date. We describe the uncovering of a protein with an estimated size of between 61-68 kDa from local RCMV strains which reacted with HCMV positive sera. Our findings are a first disclosure of a plausible immunological cross-reactivity between RCMV with its human counterpart which grounds substantial interest implying existence of conserved determinants between rat and human CMV polypeptides. The cross-reactive protein most likely represents an enveloped glycoprotein, though the precise identification and its degree of similarity needs to be evidently defined and further elucidated in forthcoming experiments.
Xenotransplantation has generated considerable interest as a potential solution to provide an ind... more Xenotransplantation has generated considerable interest as a potential solution to provide an indefinite source of islet cells for transplantation into diabetic recipients. the present study investigates the prospective use of caprine islet cellsas an alternative source of pancreatic islets for xenotransplantation. the objectives of the study were to evaluate transplanted caprine islets viability and their functionality following transplantation into diabetic mice. in this study,caprine pancreases were collected from a local slaughterhouse and transported to the laboratory by maintaining the cold chain. islets were obtained by a collagenase-based digestion. islet cell purity and viability were evaluated by dithizone and pi staining, respectively. following static incubation, an in vitro insulin secretion assay was carried out and analyzed by elisa. equal volumes of caprine islets (2500 islets equivalent) were transplanted under the kidney capsules of streptozotocin induced diabetic ...
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Papers by Nazrina Camalxaman